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www.iita.org
Biotechnology for Agriculture in Nigeria
www.iita.org
Fermentation: Production of alcohol from sorghum or sugar
cane, akanmu from corn etc.
Tissue Culture: Small plant parts placed on nutrient media
containing hormones.
Biotechnology:- Manipulation of biological elements (plant or
animal) to develop new products
www.iita.org
Modern Biotechnology;
Genetic engineering;
R-DNA technology;
Transformation:- A gene is taken from one organism and inserted in
another to give the latter the desired trait.
Transgenic is produced. :-
Genomics: Sequences of nucleotides that make up DNA.
www.iita.org
www.iita.org
According to International Service for the Acquisition of Agric-
biotech Applications – ISAAA in 2011
GM adoption was by 16.7 million farmers
On 160 million hectares
In 29 countries: 10 developed
: 19 developing
In Africa on 2.5 million hectares
Crops:
Corn
Soybean
Cotton
Canola
www.iita.org
In Nigeria Agriculture is characterized by:
Low Productivity
– Small Holdings, subsistence
– Poor soils
– Mercy of the rains
– Limited access to irrigation facilities
– Unimproved planting materials
– Diseases, Pests, Drought, Heat, Weeds
– Storage and Transportation
– Inadequate funds
– Limited mechanisation (Inadequate tools)
– Ageing farming population
www.iita.org
Major Food Crops
Tubers:
• Cassava, Yams, Potatoes, Cocoyam
Grains:
Maize, Rice, Sorghum, Millet, Cowpea, Soybean,
Bambara, Pigeonpea, Phaseolus beans
Plantains/Bananas
Fruits and vegetables
Oil Palm/ Coconut/ Groundnut
www.iita.org
Planting yam in mounds
Yam production and
marketing
www.iita.org
At IITA tissue culture is being applied to facilitate crop improvement
In plantain/banana:- Black sigatoka disease
www.iita.org
Plantain/Banana
Constraints:- Black sigatoka - 40% yld loss;
- Banana streak virus – 60% yld loss;
- Weevils
- Nematodes
- Fusarium wilt/ Bacterial wilt
- Banana die back virus
B l a c k S i g a t o k a B a c t e r i a l w i l t F u s a r i u m w i l t
D i s e a s e s o f b a n a n a a n d p l a n t a i n
www.iita.org
Embryo rescue of hybrid seeds
Embryo rescue for hybrid seed
www.iita.org
- Mostly grown and consumed in SSA
- High protein content in grains up to 25%;
- Hence cheap source of protein;
- Approx 3.3 m tonnes on 9.8 m ha;
- Average grain yield is 600 kg/ha;
- Potential grain yield is upto 2.0 t/ha.
Cowpea – called beans in Nigeria
www.iita.org
Biotic contsraints:– Aphids;
Flower bud thrips;
Legume pod borer;
Pod sucking bugs;
Grain Weevil;
Bacterial blight;
Fusarium wilt;
Striga gesnerioides.
Cowpea
www.iita.org
An intractable pest is Maruca vitrata
www.iita.org
Maruca larva
Screened over 10,000
accessions for resistance;
Led to efforts at
developing GM cowpea
with Bt gene;
Cowpea with Bt gene
now available.
www.iita.org
Bt cowpea has been evaluated under confined field trials
Bt gene efficacious against Maruca vitrata
Further evaluations in B. Faso and Ghana
Plans to transfer Bt gene to good genetic background
Marker assisted selection to facilitate
www.iita.org
Anoplocnemis curvipes
Damaged pods
Pod sucking bugs
www.iita.org
Benefits of Biotechnology
• Rapid multiplication of new clones/varieties
• Prolonging Shelf Life of Fruits, Vegetables and Flowers
• Extend Crop Area and Season
– Stress Tolerance - Drought, Acidity, Salinity, Heat,
Water logging
• More Nutritious Food
• Healthy Produce, Low Toxins
• Pharmaceutical Proteins
• Clean Up of Environment
• Industrial Products
• Value-Added Products
www.iita.org
Improved food safety
Improving Cassava for Nutrition, Health and Sustainable Development - BioCassava Plus, a
multi-disciplinary team of scientist- Ohio State University, USA and DDPSC - Acyanogenic
cassava
www.iita.org
Food fortification
• Rice for High Beta Carotene, Vitamin E and
Enhanced Iron and Zinc bioavailability -
ProVitaMinRice Consortium, Albert-Ludwigs-
Universität Freiburg, Germany
www.iita.org
Both are derived from same ancestor, Brassica oleracea. They were
devoped over several years and generations through breeding and
selection. With bioengineering can be made more precisely over a
short period of time. (Bradford et al. 2004)
Cauliflower
Broccoli
www.iita.org
Recombinant technology
Conventional technology
Gene source
Unlimited
Usually limited to relatives within
species; could also between genera
Usually one or a few known
genes
Usually many blocks of genes of
unknown identity
Gene flow can be monitored
during subsequent generations
Individual gene flow can be
monitored
Location of genes
Random into recipient genome Normally – but not always – genes
remain in sites in which they evolved -
Source: Boulter, 1995
www.iita.org
Marker assisted breeding using DNA in cassava and cowpea
Cowpea: Aphid resistance
Bacterial blight resistance
Striga resistance
Drought tolerance
www.iita.org
Is Nigeria prepared for GMOs?
Some challenges
Research infrastructure:-
Few trained scientists in molecular biology
Only few laboratories exist with facilities for biotech
research – SHESTCO was to be a flagship
Virtually all needed reagents have to be imported
Good news is possibility for outsourcing some services
www.iita.org
Is Nigeria prepared for GMOs?
Some challenges
Complex crop combinations in farmers’ fields make certain
GMO crops unattractive
Only a few GM crops are available for planting
Likely increase in cost of procuring seeds for planting
Limited access to inputs for more productive farming
Insufficient information on GMOs
Fear of consumers attitude to produce
IPR issues
Biosafety regulations
www.iita.org
Thank You for Listening

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B4FA 2012 Nigeria: Biotechnology for Agriculture in Nigeria - Christian Fatokun

  • 2. www.iita.org Fermentation: Production of alcohol from sorghum or sugar cane, akanmu from corn etc. Tissue Culture: Small plant parts placed on nutrient media containing hormones. Biotechnology:- Manipulation of biological elements (plant or animal) to develop new products
  • 3. www.iita.org Modern Biotechnology; Genetic engineering; R-DNA technology; Transformation:- A gene is taken from one organism and inserted in another to give the latter the desired trait. Transgenic is produced. :- Genomics: Sequences of nucleotides that make up DNA.
  • 5. www.iita.org According to International Service for the Acquisition of Agric- biotech Applications – ISAAA in 2011 GM adoption was by 16.7 million farmers On 160 million hectares In 29 countries: 10 developed : 19 developing In Africa on 2.5 million hectares Crops: Corn Soybean Cotton Canola
  • 6. www.iita.org In Nigeria Agriculture is characterized by: Low Productivity – Small Holdings, subsistence – Poor soils – Mercy of the rains – Limited access to irrigation facilities – Unimproved planting materials – Diseases, Pests, Drought, Heat, Weeds – Storage and Transportation – Inadequate funds – Limited mechanisation (Inadequate tools) – Ageing farming population
  • 7. www.iita.org Major Food Crops Tubers: • Cassava, Yams, Potatoes, Cocoyam Grains: Maize, Rice, Sorghum, Millet, Cowpea, Soybean, Bambara, Pigeonpea, Phaseolus beans Plantains/Bananas Fruits and vegetables Oil Palm/ Coconut/ Groundnut
  • 8. www.iita.org Planting yam in mounds Yam production and marketing
  • 9. www.iita.org At IITA tissue culture is being applied to facilitate crop improvement In plantain/banana:- Black sigatoka disease
  • 10. www.iita.org Plantain/Banana Constraints:- Black sigatoka - 40% yld loss; - Banana streak virus – 60% yld loss; - Weevils - Nematodes - Fusarium wilt/ Bacterial wilt - Banana die back virus B l a c k S i g a t o k a B a c t e r i a l w i l t F u s a r i u m w i l t D i s e a s e s o f b a n a n a a n d p l a n t a i n
  • 11. www.iita.org Embryo rescue of hybrid seeds Embryo rescue for hybrid seed
  • 12. www.iita.org - Mostly grown and consumed in SSA - High protein content in grains up to 25%; - Hence cheap source of protein; - Approx 3.3 m tonnes on 9.8 m ha; - Average grain yield is 600 kg/ha; - Potential grain yield is upto 2.0 t/ha. Cowpea – called beans in Nigeria
  • 13. www.iita.org Biotic contsraints:– Aphids; Flower bud thrips; Legume pod borer; Pod sucking bugs; Grain Weevil; Bacterial blight; Fusarium wilt; Striga gesnerioides. Cowpea
  • 14. www.iita.org An intractable pest is Maruca vitrata
  • 15. www.iita.org Maruca larva Screened over 10,000 accessions for resistance; Led to efforts at developing GM cowpea with Bt gene; Cowpea with Bt gene now available.
  • 16. www.iita.org Bt cowpea has been evaluated under confined field trials Bt gene efficacious against Maruca vitrata Further evaluations in B. Faso and Ghana Plans to transfer Bt gene to good genetic background Marker assisted selection to facilitate
  • 18. www.iita.org Benefits of Biotechnology • Rapid multiplication of new clones/varieties • Prolonging Shelf Life of Fruits, Vegetables and Flowers • Extend Crop Area and Season – Stress Tolerance - Drought, Acidity, Salinity, Heat, Water logging • More Nutritious Food • Healthy Produce, Low Toxins • Pharmaceutical Proteins • Clean Up of Environment • Industrial Products • Value-Added Products
  • 19. www.iita.org Improved food safety Improving Cassava for Nutrition, Health and Sustainable Development - BioCassava Plus, a multi-disciplinary team of scientist- Ohio State University, USA and DDPSC - Acyanogenic cassava
  • 20. www.iita.org Food fortification • Rice for High Beta Carotene, Vitamin E and Enhanced Iron and Zinc bioavailability - ProVitaMinRice Consortium, Albert-Ludwigs- Universität Freiburg, Germany
  • 21. www.iita.org Both are derived from same ancestor, Brassica oleracea. They were devoped over several years and generations through breeding and selection. With bioengineering can be made more precisely over a short period of time. (Bradford et al. 2004) Cauliflower Broccoli
  • 22. www.iita.org Recombinant technology Conventional technology Gene source Unlimited Usually limited to relatives within species; could also between genera Usually one or a few known genes Usually many blocks of genes of unknown identity Gene flow can be monitored during subsequent generations Individual gene flow can be monitored Location of genes Random into recipient genome Normally – but not always – genes remain in sites in which they evolved - Source: Boulter, 1995
  • 23. www.iita.org Marker assisted breeding using DNA in cassava and cowpea Cowpea: Aphid resistance Bacterial blight resistance Striga resistance Drought tolerance
  • 24. www.iita.org Is Nigeria prepared for GMOs? Some challenges Research infrastructure:- Few trained scientists in molecular biology Only few laboratories exist with facilities for biotech research – SHESTCO was to be a flagship Virtually all needed reagents have to be imported Good news is possibility for outsourcing some services
  • 25. www.iita.org Is Nigeria prepared for GMOs? Some challenges Complex crop combinations in farmers’ fields make certain GMO crops unattractive Only a few GM crops are available for planting Likely increase in cost of procuring seeds for planting Limited access to inputs for more productive farming Insufficient information on GMOs Fear of consumers attitude to produce IPR issues Biosafety regulations