2,dna replication

Chen Yonggang Zhejiang University Schools of Medicine Biochemistry

DNA Replication-Conservation of Information ,[object Object],[object Object],[object Object],[object Object],[object Object]

DNA is replicated in a semi-conservative manner ,[object Object],McKee 18.2

Each separated DNA strand is duplicated to give two new double helices.

DNA semiconservative replication ,[object Object]

The process which appeared simple initially is complex ,[object Object],[object Object],[object Object],McKee 17.16

To allow the replication to occur supercoiling must be relaxed ,[object Object],[object Object],[object Object]

The first step in replication involves oriC ,[object Object],[object Object],[object Object],[object Object]

dnaA allows binding of two other important proteins ,[object Object],[object Object],[object Object]

SSB, a single strand binding tetramer stabilizes the initiation complex McKee 18.7

DNA replication involves many enzymes ,[object Object],[object Object]

At the replication fork two strands are managed differently ,[object Object],[object Object],[object Object]

The replication fork McKee 18.6

DNA Polymerase III holoenzyme contains 10 distinct types of subunits ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

The Pol III synthesizes DNA from dNTPs McKee 18.3

The Dimer moves in one direction and synthesizes 5’-3’ ,[object Object],[object Object],[object Object]

DNA polymerase III forms phosphodiester bonds ,[object Object],[object Object],[object Object]

Synthesis of a phosphodiester bond

DNA Polymerase III is at the center of Replication McKee 18.8

While DNA polymerase III does the replicating, DNAP I cleans up ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

The three activities are on one polypeptide ,[object Object],[object Object]

As Pol III finishes, Pol I goes to work ,[object Object],[object Object],[object Object]

Supercoiling was taken out by dnaB, DNA gyrase replaces it ,[object Object],[object Object]

DNA ligase seals the Okazaki fragments and the completed double helical DNA ,[object Object],[object Object],[object Object],[object Object]

Eucaryotic Replication is similar to that of procaryotes ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Eucaryotic replication is distinct from that of procaryotes ,[object Object],[object Object],[object Object],[object Object],[object Object]

Initiation of replication occurs at multiple ori ,[object Object],[object Object],[object Object],[object Object],[object Object]

Binding of initiation factors to the lagging strand differs ,[object Object],[object Object],[object Object],[object Object],[object Object]

Finishing and sealing of the lagging strand is different ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Telomeres are GC rich self-complementary sequences at chromosome ends ,[object Object],[object Object],[object Object],[object Object],[object Object]

2,dna replication

Empfohlen

Empfohlen

Weitere ähnliche Inhalte

Was ist angesagt?

Was ist angesagt? (20)

Ähnlich wie 2,dna replication

Ähnlich wie 2,dna replication (20)

Mehr von Thumz Industries (MBBS)

Mehr von Thumz Industries (MBBS) (12)

2,dna replication