QUANTITATIVE LAWS June 13 -June 24

1. Entropic effects in
chromatin folding
Alberto Colliva
Quantitative Laws II16/06/2016

2. How is DNA packing organized?
Tark-Dame et. al., PLOS Comp. Biol., 2009
What is driving the 3D
organization?
Polymers
3C techniques
Dixon et. al., Nature, 2011

3. GC and Hi-C integration
Different cell lines and Hi-C experiments
Juicebox
Hi-C data suffer major bias
S. Rao et. al., «Juicebox», Cell, 2014 GC profile chromosome 2
Chromosome coordinates: X ,Y
YX

4. Enrichment in GC similar contats
HindIII NcoI
GC content (5 kb)
GCcontent(5kb)
GCcontent(5kb)
GC content (5 kb)
Data from: Lieberman-Aiden et. Al., Science. 2009
Log(Survivalfunction)
Log(Survivalfunction)
5 kb resolution windows
Two different restriction enzyme

5. GC content as a flexibility indicator
P. Cook D. Marenduzzo, J Cell Biol., 2009
A mixture of two sets of polymers show the emergence of
segregation.
Molecular dynamics simulation
GC content (5 kb)
GCcontent(5kb)
flexiblestiff

6. Acknowledgement
Michele Caselle
Andrea Mazzolini
Matteo Osella
Antonio Rosanova