This document discusses the analytical technique of bioluminescence. It begins by defining bioluminescence as the production and emission of light by living organisms through a chemical reaction involving luciferin and luciferase. This reaction produces light energy. Bioluminescence assays can be used to measure cell proliferation, apoptosis, drug metabolism, and kinase activity. The document then explains the principle behind bioluminescence and provides steps for a bioluminescence procedure using cell lysis and a luminometer to measure light emission. Finally, some applications of bioluminescence are mentioned such as food testing, imaging, and medical research.

1. Presented by: Jagruti Ravindra Sutar.
M. Pharm. 1st year. Sem I. Pharmaceutics
Roll No- 13
Subject- Modern Pharmceutical Analytical Technique

2.  Introduction
 Principle
 Procedure
 Instrumentation
 Application

3.  Bioluminescence is the production and emission of
light by a living organism. It is a form of
chemiluminescence.
 Bioluminescence occurs widely in marine
vertebrates and invertebrates, as well as in some
fungi, microorganisms including some
bioluminescent bacteria and terrestrial
invertebrates such as fireflies.
 Bioluminescence assays involve the use of the
property of bioluminescence for measuring cell
proliferation, apoptosis, drug metabolism, kinas
activity, etc.

4.  Bioluminescence is a form of chemiluminescence
where light energy is released by a chemical
reaction.
 This reaction involves a light-emitting pigment,
the luciferin , and a luciferase, the enzyme
component.
Luciferin + Oxygen Oxyluciferin + Light
energy
( AMP, PP and Carbon dioxide )
Mg++
ATP

5. Aspirate medium of cells
Rinse with PBS
Add sufficient amount of Glo-lysis buffer
Rock the plate slowly to complete coverage of the
cells
Incubate for 5 min to allow cell lysis

6. Transfer the lysate to new tube
Centrifuge for 10 min at 1000g
Place 50 µg Bright glow assay reagent into
luminometer plate
Add same volume of lysate
Measure luminescence with Luminometer

7. Fig: Luminometer
 Microplate
 Monochromater &
filters
 Detector

8. Fig: Microplate

9.  Food testing using ATP technology
 Bioluminescent imaging
 Development of new treatment for antibiotic
resistant tuberculosis
 Discovery of green protein

11. THANK
YOU