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TUTOR HEMATOLOGI PEMERIKSAAN HITUNG JENIS LEKOSIT ( LEUCOCYTE DIFFERENTIAL COUNT) Oleh dr. Faizah Yunianti dr Yetty Hernaningsih, Sp.PK

PENDAHULUAN(1) ,[object Object],[object Object],[object Object],[object Object],[object Object]

PENDAHULUAN(2) ,[object Object],[object Object],[object Object]

Harga Normal (persentase rata-rata)

PEMERIKSAAN HITUNG JENIS LEKOSIT manual otomatik ,[object Object],[object Object],[object Object],[object Object],Metode Resistensi Elektronik/ Impedans/ Metode Optikal Kombinasi metode impedans & optikal

PEMERIKSAAN OTOMATIK Metode resistensi elektronik/ impedan/ tahanan listrik Metode Optikal Light scatter Light Scatter & Absorbsi Kombinasi impedan & Metode optikal Direct Current & Radio Frequensi Direct Current (DC ) sitokimia fluoresensi

Otomatis Metode Alat Impedans Coulter (DC) Sysmex NE-8000 (DC&RF) Light scatter Cell-Dyn 3200 Light Scatter & Absorbsi Sysmex XE 2100 Tehnicon H1 Advia 2120 Kombinasi Impedans & Light Scatering Cobas Helios 5 Diff

Metode Resistensi Elektronik/ Impedans/Tahanan Listrik

Metode Resistensi Elektronik/ Impedans/Tahanan Listrik ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Metode Resistensi Elektronik/ Impedansi/Tahanan Listrik ,[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Direct Current & Radio Frequency ,[object Object],[object Object],[object Object],[object Object],[object Object]

Direct Current & Radio Frequency

Direct Current & Radio Frequensi

Metode Optikal ,[object Object],[object Object],[object Object],[object Object]

Light Scatter ,[object Object],[object Object],[object Object],[object Object],[object Object]

Pengukuran scatter light 4 sudut ( multi angle polarized scatter separation/MAPPS) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

MONONUCLEAR-POLYMORPHONUCLEAR SCATTER ,[object Object],[object Object],[object Object],[object Object]

NEUTROPHIL-EOSINOPHIL SCATTER ,[object Object],[object Object],[object Object]

MONONUCLEAR SCATTER ,[object Object],[object Object],[object Object],[object Object]

Fluoresensi ( Light Scatter & Absorbsi) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Fluoresensi ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object],[object Object]

Memisahkan Lekosit dari NRBC ,[object Object],[object Object],[object Object]

Memisahkan Lekosit dari NRBC ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Scattergram NRBC ,[object Object],[object Object]

Channel Basofil (WBC/BASO Scattergram) ,[object Object],[object Object],[object Object],[object Object]

Immatur Granulosit Netrofil ,[object Object],[object Object]

IMI Scattergram ,[object Object],[object Object],[object Object]

Sitokimia ,[object Object],[object Object],[object Object],[object Object]

Sitokimia ,[object Object],[object Object]

Lyse RBC Fix WBC Stain peroxidase = positive WBC ,[object Object],[object Object],[object Object],Chromogen (4-chloro-1naphtol) Substrat (H 2 O 2 ) Blood 12 μ L H 2 O 2 + 4-chloro-1-napthol --------------------  presipitat warna gelap dalam sel Peroksidase seluler

Sitogram WBC/ Peroxidase Channel Intensitas pewarnaan U K U R A N

Sitokimia Basofil ,[object Object],[object Object],[object Object],[object Object],[object Object]

Sitokimia Basofil ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Blood 12 μ L

Large unstained Cell (LUC) ,[object Object],[object Object],[object Object]

PERBANDINGAN HITUNG JENIS LEKOSIT SECARA MANUAL DAN OTOMATIK CARA KEUNTUNGAN KERUGIAN MANUAL ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],OTOMATIK ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Scattergram Coulter STKS (DC)

Fluoresensi ,[object Object],[object Object],[object Object]

Fluoresensi ,[object Object],[object Object],[object Object],[object Object]

Manual ,[object Object],[object Object],[object Object],[object Object]

Gambar 1. Prosedur (a-d) pembuatan hapusan darah tepi untuk hitung jenis lekosit

a. Pembuatan hapusan darah ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

b. Pewarnaan ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

c. Pemeriksaan hapusan darah ,[object Object],[object Object],[object Object],[object Object],[object Object]

d. Perhitungan jenis lekosit ,[object Object],[object Object]

Metode Analisis Basofil/ Lobularitas Inti

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