ENTOLAB-loppuseminaari: hyönteiskasvatuksen edistäminen Etelä-Pohjanmaalla 4.12.18 Seinäjoki

2. Research and development efforts on optimizing
key parameters in industrial insect production
NJF 2018, 4-5 December Anton Gligorescu, Consultant – Insect Production, DTI

3. The Danish Technological Institute was founded in 1906
For more than 100 years, DTI has ensured the translation of the latest knowledge and technology
into real value for Danish and international businesses.

4. Self-owned and not-for-profit
More than 1,000 innovative employees, state of the art equipment and facilities
as well as a strong global network.

5. Materials,
Management
Packaging and
logistics
Functional Coating
Metal and Surface
Technology
Plastics Technology
Product Development
Tribology
Materials
Executive Vice
President
Mikkel Agerbæk
Building and
Construction,
Management
Concrete
Glass and Windows
Buildings and
Environment
Masonry
Wood and
Biomaterials
Building and
Construction
Executive Vice
President
Mette Glavind
Energy and Climate,
Management
Automobile
Technology
Energy Efficiency and
ventilation
Installation and
Calibration
Refrigeration and
Heat Pump
Technology
Pipe Centre
Transport and Electric
Systems
Energy and
Climate
Executive Vice
President
David Tveit
Life Science,
Management
DTI Oil & Gas
Laboratory for
Chemistry and
Microbiology
Bioengineering and
Environmental
Technology
Biotek
Life Science
Executive Vice
President
Sune D. Nygaard
AgroTech,
Management
Bioressources and
Biorefinery
Plant Technology
Field Trials,
Technology and
Analysis
Software
Development
Food Technology
AgroTech
Executive Vice
President Mikael
Poulsen
DMRI, Business
Development
Food Safety
Meat Technology
Measuring Systems
and Data Integration
Slaughterhouse
Technologies
Automation
DMRI
Executive Vice
President
Lars Hinrichsen
Business and Society,
Management
Policy and Business
Development
Ideas and Innovation
Training
Business and
Society
Executive Vice
President
Jane Wickmann
Board of Representatives
Board of trustees
Subsidiaries
Danfysik A/S
Teknologisk
Institut
AB Sverige
DTI Polska
Sp. z o. o.
Teknologisk
Innovation A/S
Dancert A/S
Danish
Technological
Institute Spain,
S.L.
Technological Institute
President Søren Stjernqvist
Production,
Management
Metrology and
Quality Assurance
Nano Production and
Micro Analysis
Robot Technology
Health and Human
Interaction
Technologies
Production
Executive Vice
President
Anne-Lise H. Lejre
Insect value
chain @DTI

6. Focus areas of DTI Insect Production Team

7. Danish public R&D projects 2017/18 (>100k €)
DTI lead highlighted in bold
Mealworms
 inVALUABLE (3.7M €, IFD)
 SUSMEAL (1.1M €, IFD, Eurostars)
 VALIN (175k €, GUDP)
 Wholi Foods (~100k €, IFD)
 ENORM (~100k €, IFD)
 NLF (135k €, IFD)
 Ikadan (135k €, IFD)
Black Solider Fly (BSF)
 WICE (390k €, MUDP)
 SUPERIOR (375k €, MUDP)
 Green Biorefining (360k €, F&I)
 BIOCAST (~100k €, Interreg/EU)
Crickets
 GREEINSECT (1.34M €, DANIDA)
 Syngja (200k €, IFD)
 Synjga2 (185 €, FFI)
>7M € portfolio

8. Black Soldier Fly (BSF) (Hermetia illucens) and
Common mealworm (Tenebrio molitor)
 Assessment of the suitability of different feeding substrates
 Optimization of rearing system
 Optimization of reproductive output
 GHG and heat production
 Computational Fluid Dynamic (CFD) modelling

9. Generic production process - Optimization

10. Performance of BSF larvae (BSFL) reared on
different substrates
Substrates tested
(Project)
Applicability FCR (dry matter)
Grass, lucerne and red clover
(Green Biorefining)
N/A (tested individually at
100% inclusion level)
-
Spent grains + different carbohydrate-
rich by-products (SUPERIOR)
Medium-high -
Dairy by-products (SUPERIOR) Medium -
Beach cast + spent grains (BIOFISK) Low 7-10
Agro-industrial by-products (BIOCAST) High 2.3-3.2
Catering waste (WICE) High 1.6-2.4
Chicken feed High 1.6-2.8

11. Performance of BSFL reared on different substrates -BIOCAST
Diet 1 Diet 2 Diet 3 Diet 4
Beach Cast 0.10 0.05 - -
Spent Grain 0.15 0.20 0.19 0.25
Apple pomas - 0.10 - 0.10
Vinter wheat 0.20 0.10 0.11 0.10
Rapeseed cake - 0.02 - 0.02
Beet tops 0.05 - 0.02 -
Sprouts from malts 0.05 0.10 0.03 0.10
Butter cakes 0.05 - 0.03 -
Substrates Ratio

12. Optimization of BSFL reared on chicken feed
FCR (dry matter basis) of BSFL batches Larval biomass (dry matter basis) of BSFL batches
Feeding amount, feeding frequency, feed conditioning (e.g. fermentation), larval density
-
2
4
6
8
10
12
14
16
18
20
22
24
26
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
kg/m³
-
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
FCR

13. Reproduction of BSF – optimizing fly density
Egg production per cage at different fly densities at 31°C
(avg±sd)
8.8
11.8
8.4
DENSITY 1: 1000 DENSITY 2: 1600 DENSITY 3:2300
Eggs/cage(g)
Adult density
Egg production per cage at different fly densities at 28°C
(avg±sd)
18.5
16.2
-
5.0
10.0
15.0
20.0
25.0
Density 2: 1300 Density 1: 1000
Eggs/cage(g)
Adult density
Temperature (ºc) Density (adults/m³) Eggs (g) Nenates (kg) Larvae (kg)
28 6,000 46.4 3.7 211
31 7,200 29.5 2.4 134
Production estimation for 1m³ reproduction cage

14. inVALUABLE - Mealworm
Insect Value Chain in a Circular Bioeconomy
Duration: 2017-2019 (36 months)
Total budget: 3.7M EUR (2.5M EUR investment from Innovation Fund Denmark)
The vision of inVALUABLE is to create a sustainable resource-efficient
industry for animal production based on insects (focus on T. molitor)
The partners span the entire value chain and include entrepreneurs, experts
in biology, biotech, automation, processing and food technology and safety. This
interaction of competences is key to lifting insect production to an
industrial level

15. inVALUABLE: Focal areas - mealworm
Insect Value Chain in a Circular Bioeconomy

16. 0
100
200
300
400
0.11 0.21 0.42 0.84
Density (beetles/cm
2
)
Totalnumberoflarvae
0
5
10
15
20
0.11 0.21 0.42 0.84
Density (beetles/cm
2
)
Larvae/female/day
0
5
10
15
20
1 2 3 4 6
Reproduction period (days)
Totalnumberoflarvae
0
5
10
15
20
1 2 3 4 6
Reproduction period (days)
Larvae/female/day
Tenebrio reproduction @ lab-scale

17. Tenebrio larval growth and
respiration @ lab-scale
T. molitor T. molitor

18. Measurement of key parameters @ pilot-scale
CO2 and other GHG
Day 46-47 (high resolution)
GHG emission as CO2-equiv. of T. molitor larvae (age 36-48)

19. CFD modelling: Simulation of key parameters
@ large-scale
3D virtual models simulating,
e.g. temperature (shown) or
gases (CO2 and other GHG,
not shown)

20. THANK YOU!
Anton Gligorescu
E: ANGL@DTI.DK
M: +45 7220 2904