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Greenhouse
Performance
of Round 3
Containers
Kenneth G McCabe
Department of
Horticulture
Iowa State University
Outline for Presentation
• Container Aesthetics and Shuttle Tray Experiments
• Greenhouse Nutrition Trial
• Take-home Messages
• Acknowledgements and Questions
Container Aesthetics Experiment
Gerbera (10 weeks) Asparagus Fern (15 weeks)
Gerbera grown
for 10 weeks
Containers
used for 10
weeks
PLA/Soy
(60/40)
PLA/Soy/BioRes
(50/30/20)
PLA/Soy/BioRes
(55/35/10)
PLA/BioRes (BR)
(80/20)
PLA/Lignin
(90/10)
rPLA
(100)
PHA/DDGS
(80/20)
Petroleum
plastic
PUR-coated
Paper-fiber
Asparagus Fern
15 weeks old
PLA/BioRes containers with
and without colorant
Blue Colorant Green Colorant No Color Added
Shuttle Tray Experiment
Marigold
‘Honeycomb’
grown for 7
weeks
Bioplastic
Containers Only
PLA/Lignin (90/10)
PLA/Soy/BR (50/30/20)
PLA/Soy/BR (55/35/10)
PLA/Soy (60/40)
PHA/DDGS (80/20)
rPLA (100)
PLA/BioRes (80/20)
PLA/BioRes (80/20)-Green Colorant
PLA/BioRes (70/30)-Blue Colorant
Petroleum Plastic
Container Nutrition Trial
Marigold
‘Honeycomb’
5 weeks of
culture
5 Fertilizer
Treatments
4.5” Containers Evaluated
(% by weight)
PLA/BioRes (BR) (80/20)
PLA/Lignin (90/10)
PLA/Soy/BR (50/30/20)
PLA/Soy (60/40)
PHA/DDGS (80/20)
PLA/Soy/BR (55/35/10)
rPLA (100)
Petroleum Plastic - polypropylene
Fertilizer Treatments
Wk 1 Wk 2 Wk 3 Wk 4 Wk 5
(0)
(60)
(113)
(226)
(452)
0 0 0 0 0
100 100 0 0 0
75 75 75 75 75
150 150 150 150 150
300 300 300 300 300
Fertilizer (ppm N) supplied from a 16.6N–5P–16.3K water-soluble fertilizer
Applied
N (mg)
1)
2)
3)
4)
5)
Petroleum Plastic
PLA/Soy/BR (50/30/20)
PLA/Soy (60/40)
PLA/Soy/BR (55/35/10)
rPLA (100%)
PHA/DDGS (80/20)
PLA/Lignin (90/10)
PLA/BR (80/20)
Fertilizer Trt (mg of N)
0 6 113 226 452Container Nutrition Trial
Shoot Dry Weight (g)
Fertilizer Treatment (mg of N)
Container Type 0 60 113 226 452
PLA/BR (80/20) 0.8 Cd 3.2 BCc 3.4 ABc 4.7 Ab 6.1 Aa
PLA/Lignin 0.8 Cd 2.9 BCc 3.9 ABb 5.1 Aa 5.7 Aa
PLA/Soy/BR (50/30/20) 2.6 Ac 4.6 Aab 4.1 Ab 5.9 Aa 5.4 Aab
PLA/Soy (60/40) 2.6 Ad 3.6 Bcd 4.5 Abc 5.3 Aab 6.3 Aa
PHA/DDGS 0.5 Cc 2.4 Cb 2.9 Bb 4.9 Aa 5.5 Aa
PLA/Soy/BR (55/35/10) 1.8 Bd 3.8 ABc 4.2 Abc 5.5 Aab 5.9 Aa
rPLA 0.4 Cd 3.1 BCc 3.6 ABc 4.9 Ab 6.3 Aa
PP container 0.7 Cd 3.0 BCc 3.8 ABbc 4.8 Ab 6.7 Aa
Container Nutrition Trial
Petroleum Plastic
PLA/Soy/BR (50/30/20)
PLA/Soy (60/40)
PLA/Soy/BR (55/35/10)
Fertilizer Treatment (mg of N)
0 60 113 226 452Container Nutrition Trial
Petroleum Plastic
PLA/Soy/BR (50/30/20)
PLA/Soy (60/40)
PLA/Soy/BR (55/35/10)
Fertilizer Treatment (mg of N)
0 60 113 226 452Container Nutrition Trial
Shoot Dry Weight (g)
Fertilizer Treatment (mg of N)
Container Type 0 60 113 226 452
PLA/BR (80/20)
PLA/Lignin
PLA/Soy/BR (50/30/20) 2.6 Ac 4.6 Aab 4.1 Ab 5.9 Aa 5.4 Aab
PLA/Soy (60/40) 2.6 Ad 3.6 Bcd 4.5 Abc 5.3 Aab 6.3 Aa
PHA/DDGS
PLA/Soy/BR (55/35/10) 1.8 Bd 3.8 ABc 4.2 Abc 5.5 Aab 5.9 Aa
rPLA
PP container 0.7 Cd 3.0 BCc 3.8 ABbc 4.8 Ab 6.7 Aa
Container Nutrition Trial
Petroleum Plastic
PLA/BR (80/20)
PLA/Lignin (90/10)
rPLA (100)
Fertilizer Treatment (mg of N)
0 60 113 226 452
Container Nutrition Trial
Shoot Dry Weight (g)
Fertilizer Treatment (mg of N)
Container Type 0 60 113 226 452
PLA/BR (80/20) 0.8 Cd 3.2 BCc 3.4 ABc 4.7 Ab 6.1 Aa
PLA/Lignin 0.8 Cd 2.9 BCc 3.9 ABb 5.1 Aa 5.7 Aa
PLA/Soy/BR (50/30/20)
PLA/Soy (60/40)
PHA/DDGS
PLA/Soy/BR (55/35/10)
rPLA 0.4 Cd 3.1 BCc 3.6 ABc 4.9 Ab 6.3 Aa
PP container 0.7 Cd 3.0 BCc 3.8 ABbc 4.8 Ab 6.7 Aa
Container Nutrition Trial
Petroleum Plastic
PHA/DDGS (80/20)
Fertilizer Treatment (mg of N)
0 60 113 226 452
Container Nutrition Trial
Shoot Dry Weight (g)
Fertilizer Treatment (mg of N)
Container Type 0 60 113 226 452
PLA/BR (80/20)
PLA/Lignin
PLA/Soy/BR (50/30/20)
PLA/Soy (60/40)
PHA/DDGS 0.5 Cc 2.4 Cb 2.9 Bb 4.9 Aa 5.5 Aa
PLA/Soy/BR (55/35/10)
rPLA
PP container 0.7 Cd 3.0 BCc 3.8 ABbc 4.8 Ab 6.7 Aa
Container Nutrition Trial
Petroleum Plastic
PLA/Soy/BR (50/30/20)
PLA/Soy (60/40)
PLA/Soy/BR (55/35/10)
rPLA (100%)
PHA/DDGS (80/20)
PLA/Lignin (90/10)
PLA/BR (80/20)
Fertilizer Trt (mg of N)
0 6 113 226 452Container Nutrition Trial
Shoot Nitrogen (N) characteristics
Fertilizer Treatment (mg of N)
Container Type 0 60 113 226 452
Shoot N (%)
PLA/BR (80/20) 1.2 Cc 1.4 Cc 2.3 Cb 2.3 CDb 3.4 BCa
PLA/Lignin 0.7 Cd 1.3 Cc 2.1 Cb 3.2 BCa 3.5 BCa
PLA/Soy/BR (50/30/20) 4.5 Aa 4.0 Aa 4.6 Aa 4.1 Aa 4.4 Aa
PLA/Soy (60/40) 2.7 Ba 2.8 Ba 3.3 Ba 3.1 BCa 3.6 A-Ca
PHA/DDGS 0.7 Cc 1.0 Cc 1.6 Cb 1.9 Db 3.0 Ca
PLA/Soy/BR (55/35/10) 4.1 Aa 3.0 Bb 3.7 Bab 3.5 ABab 4.1 ABa
rPLA 0.7 Cd 1.3 Ccd 1.9 Cc 2.7 B-Db 3.5 BCa
PP container 0.8 Cc 1.3 Cc 2.2 Cb 2.5 CDb 3.3 Ca
Shoot N content (mg)
PLA/BR (80/20) 9 Ce 43 Cd 71 Cc 117 DEb 207 ABa
PLA/Lignin 6 Ce 38 Cd 80 Cc 139 CDb 216 ABa
PLA/Soy/BR (50/30/20) 120 Ab 181 Aab 199 Aab 231 Aa 220 ABa
PLA/Soy (60/40) 66 Bd 93 Bd 129 Bc 163 BCb 264 Aa
PHA/DDGS 4 Ce 25 Cd 46 Cc 87 Eb 163 Ba
PLA/Soy/BR (55/35/10) 72 Bc 109 Bbc 146 Bb 194 Ba 236 ABa
rPLA 3 Cd 40 Cc 65 Cc 117 DEb 205 ABa
PP container 5 Ce 40 Cd 74 Cc 128 Db 221 ABa
Petroleum Plastic
PLA/Soy/BR (50/30/20)
PLA/Soy (60/40)
PLA/Soy/BR (55/35/10)
Fertilizer Treatment (mg of N)
0 60 113 226 452
Container Nutrition Trial
Shoot Nitrogen (N) characteristics
Fertilizer Treatment (mg of N)
Container Type 0 60 113 226 452
Shoot N (%)
PLA/BR (80/20)
PLA/Lignin
PLA/Soy/BR (50/30/20) 4.5 Aa 4.0 Aa 4.6 Aa 4.1 Aa 4.4 Aa
PLA/Soy (60/40) 2.7 Ba 2.8 Ba 3.3 Ba 3.1 BCa 3.6 A-Ca
PHA/DDGS
PLA/Soy/BR (55/35/10) 4.1 Aa 3.0 Bb 3.7 Bab 3.5 ABab 4.1 ABa
rPLA
PP container 0.8 Cc 1.3 Cc 2.2 Cb 2.5 CDb 3.3 Ca
Shoot N content (mg)
PLA/BR (80/20)
PLA/Lignin
PLA/Soy/BR (50/30/20) 120 Ab 181 Aab 199 Aab 231 Aa 220 ABa
PLA/Soy (60/40) 66 Bd 93 Bd 129 Bc 163 BCb 264 Aa
PHA/DDGS
PLA/Soy/BR (55/35/10) 72 Bc 109 Bbc 146 Bb 194 Ba 236 ABa
rPLA
PP container 5 Ce 40 Cd 74 Cc 128 Db 221 ABa
Petroleum Plastic
PLA/Soy/BR (50/30/20)
PLA/Soy (60/40)
PLA/Soy/BR (55/35/10)
Fertilizer Treatment (mg of N)
0 60 113 226 452
Container Nutrition Trial
Shoot Nitrogen (N) characteristics
Fertilizer Treatment (mg of N)
Container Type 0 60 113 226 452
Shoot N (%)
PLA/BR (80/20)
PLA/Lignin
PLA/Soy/BR (50/30/20) 4.5 Aa 4.0 Aa 4.6 Aa 4.1 Aa 4.4 Aa
PLA/Soy (60/40) 2.7 Ba 2.8 Ba 3.3 Ba 3.1 BCa 3.6 A-Ca
PHA/DDGS
PLA/Soy/BR (55/35/10) 4.1 Aa 3.0 Bb 3.7 Bab 3.5 ABab 4.1 ABa
rPLA
PP container 0.8 Cc 1.3 Cc 2.2 Cb 2.5 CDb 3.3 Ca
Shoot N content (mg)
PLA/BR (80/20)
PLA/Lignin
PLA/Soy/BR (50/30/20) 120 Ab 181 Aab 199 Aab 231 Aa 220 ABa
PLA/Soy (60/40) 66 Bd 93 Bd 129 Bc 163 BCb 264 Aa
PHA/DDGS
PLA/Soy/BR (55/35/10) 72 Bc 109 Bbc 146 Bb 194 Ba 236 ABa
rPLA
PP container 5 Ce 40 Cd 74 Cc 128 Db 221 ABa
Shoot Nitrogen (N) characteristics
Fertilizer Treatment (mg of N)
Container Type 0 60 113 226 452
Shoot N (%)
PLA/BR (80/20)
PLA/Lignin
PLA/Soy/BR (50/30/20) 4.5 Aa 4.0 Aa 4.6 Aa 4.1 Aa 4.4 Aa
PLA/Soy (60/40) 2.7 Ba 2.8 Ba 3.3 Ba 3.1 BCa 3.6 A-Ca
PHA/DDGS
PLA/Soy/BR (55/35/10) 4.1 Aa 3.0 Bb 3.7 Bab 3.5 ABab 4.1 ABa
rPLA
PP container 0.8 Cc 1.3 Cc 2.2 Cb 2.5 CDb 3.3 Ca
Shoot N content (mg)
PLA/BR (80/20)
PLA/Lignin
PLA/Soy/BR (50/30/20) 120 Ab 181 Aab 199 Aab 231 Aa 220 ABa
PLA/Soy (60/40) 66 Bd 93 Bd 129 Bc 163 BCb 264 Aa
PHA/DDGS
PLA/Soy/BR (55/35/10) 72 Bc 109 Bbc 146 Bb 194 Ba 236 ABa
rPLA
PP container 5 Ce 40 Cd 74 Cc 128 Db 221 ABa
Petroleum Plastic
PLA/Soy/BR (50/30/20)
PLA/Soy (60/40)
PLA/Soy/BR (55/35/10)
Fertilizer Treatment (mg of N)
0 60 113 226 452
Container Nutrition Trial
Shoot Nitrogen (N) characteristics
Fertilizer Treatment (mg of N)
Container Type 0 60 113 226 452
Shoot N (%)
PLA/BR (80/20)
PLA/Lignin
PLA/Soy/BR (50/30/20) 4.5 Aa 4.0 Aa 4.6 Aa 4.1 Aa 4.4 Aa
PLA/Soy (60/40) 2.7 Ba 2.8 Ba 3.3 Ba 3.1 BCa 3.6 A-Ca
PHA/DDGS
PLA/Soy/BR (55/35/10) 4.1 Aa 3.0 Bb 3.7 Bab 3.5 ABab 4.1 ABa
rPLA
PP container 0.8 Cc 1.3 Cc 2.2 Cb 2.5 CDb 3.3 Ca
Shoot N content (mg)
PLA/BR (80/20)
PLA/Lignin
PLA/Soy/BR (50/30/20) 120 Ab 181 Aab 199 Aab 231 Aa 220 ABa
PLA/Soy (60/40) 66 Bd 93 Bd 129 Bc 163 BCb 264 Aa
PHA/DDGS
PLA/Soy/BR (55/35/10) 72 Bc 109 Bbc 146 Bb 194 Ba 236 ABa
rPLA
PP container 5 Ce 40 Cd 74 Cc 128 Db 221 ABa
Shoot Nitrogen (N) characteristics
Fertilizer Treatment (mg of N)
Container Type 0 60 113 226 452
Shoot N (%)
PLA/BR (80/20)
PLA/Lignin
PLA/Soy/BR (50/30/20) 4.5 Aa 4.0 Aa 4.6 Aa 4.1 Aa 4.4 Aa
PLA/Soy (60/40) 2.7 Ba 2.8 Ba 3.3 Ba 3.1 BCa 3.6 A-Ca
PHA/DDGS
PLA/Soy/BR (55/35/10) 4.1 Aa 3.0 Bb 3.7 Bab 3.5 ABab 4.1 ABa
rPLA
PP container 0.8 Cc 1.3 Cc 2.2 Cb 2.5 CDb 3.3 Ca
Shoot N content (mg)
PLA/BR (80/20)
PLA/Lignin
PLA/Soy/BR (50/30/20) 120 Ab 181 Aab 199 Aab 231 Aa 220 ABa
PLA/Soy (60/40) 66 Bd 93 Bd 129 Bc 163 BCb 264 Aa
PHA/DDGS
PLA/Soy/BR (55/35/10) 72 Bc 109 Bbc 146 Bb 194 Ba 236 ABa
rPLA
PP container 5 Ce 40 Cd 74 Cc 128 Db 221 ABa
Petroleum Plastic
PLA/BR (80/20)
PLA/Lignin (90/10)
rPLA (100)
Fertilizer Treatment (mg of N)
0 60 113 226 452
Container Nutrition Trial
Shoot Nitrogen (N) characteristics
Fertilizer Treatment (mg of N)
Container Type 0 60 113 226 452
Shoot N (%)
PLA/BR (80/20) 1.2 C 1.4 C 2.3 C 2.3 CD 3.4 BC
PLA/Lignin 0.7 C 1.3 C 2.1 C 3.2 BC 3.5 BC
PLA/Soy/BR (50/30/20)
PLA/Soy (60/40)
PHA/DDGS
PLA/Soy/BR (55/35/10)
rPLA 0.7 C 1.3 C 1.9 C 2.7 B-D 3.5 BC
PP container 0.8 C 1.3 C 2.2 C 2.5 CD 3.3 C
Shoot N content (mg)
PLA/BR (80/20) 9 Ce 43 Cd 71 Cc 117 DEb 207 ABa
PLA/Lignin 6 Ce 38 Cd 80 Cc 139 CDb 216 ABa
PLA/Soy/BR (50/30/20)
PLA/Soy (60/40)
PHA/DDGS
PLA/Soy/BR (55/35/10)
rPLA 3 Cd 40 Cc 65 Cc 117 DEb 205 ABa
PP container 5 Ce 40 Cd 74 Cc 128 Db 221 ABa
Petroleum Plastic
PHA/DDGS (80/20)
Fertilizer Treatment (mg of N)
0 60 113 226 452
Container Nutrition Trial
Shoot Nitrogen (N) characteristics
Fertilizer Treatment (mg of N)
Container Type 0 60 113 226 452
Shoot N (%)
PLA/BR (80/20)
PLA/Lignin
PLA/Soy/BR (50/30/20)
PLA/Soy (60/40)
PHA/DDGS 0.7 C 1.0 C 1.6 C 1.9 D 3.0 C
PLA/Soy/BR (55/35/10)
rPLA
PP container 0.8 C 1.3 C 2.2 C 2.5 CD 3.3 C
Shoot N content (mg)
PLA/BR (80/20)
PLA/Lignin
PLA/Soy/BR (50/30/20)
PLA/Soy (60/40)
PHA/DDGS 4 C 25 C 46 C 87 E 163 B
PLA/Soy/BR (55/35/10)
rPLA
PP container 5 C 40 C 74 C 128 D 221 AB
100 ppm N for the first 2 weeks only
(60 mg N)
Petroleumplastic PLA/Soy/BR (50/30/20)
Petroleum Plastic
PLA/Soy/BR (50/30/20)
PLA/Soy (60/40)
PLA/Soy/BR (55/35/10)
rPLA (100%)
PHA/DDGS (80/20)
PLA/Lignin (90/10)
PLA/BioRes (80/20)
Fertilizer Treatment (mg of N)
0 60 113 226 452
Container Nutrition Trial
Average Root Circling Rating (1=no root circling, 4=complete root circling)
Fertilizer Treatment (mg of N)
Container Type 0 60 113 226 452
PLA/BR (80/20) 2.1 Ab 3.6 Aa 3.8 Aa 3.9 Aa 3.5 Aa
PLA/Lignin 1.9 Ab 3.6 Aa 3.9 Aa 3.9 Aa 3.9 Aa
PLA/Soy/BR (50/30/20) 1.1 Ab 1.4 Ca 1.2 Dab 1.4 Da 1.2 Dab
PLA/Soy (60/40) 1.7 Ab 1.8 BCa 2.0 Cab 2.2 Ca 1.8 Cab
PHA/DDGS 1.4 Ac 2.2 Bb 2.6 Bab 3.1 Ba 2.6 Bab
PLA/Soy/BR (55/35/10) 1.3 Ab 1.8 BCa 1.8 Ca 1.8 CDa 1.7 Cab
rPLA 2.2 Ab 3.8 Aa 3.8 Aa 3.9 Aa 3.9 Aa
PP container 1.8 Ab 3.8 Aa 3.9 Aa 3.9 Aa 3.9 Aa
Container Nutrition Trial
Petroleum Plastic
PLA/BR (80/20)
PLA/Lignin (90/10)
rPLA (100)
Fertilizer Treatment (mg of N)
0 60 113 226 452
Container Nutrition Trial
Average Root Circling Rating (1=no root circling, 4=complete root circling)
Fertilizer Treatment (mg of N)
Container Type 0 60 113 226 452
PLA/BR (80/20) 2.1 A 3.6 A 3.8 A 3.9 A 3.5 A
PLA/Lignin 1.9 A 3.6 A 3.9 A 3.9 A 3.9 A
PLA/Soy/BR (50/30/20)
PLA/Soy (60/40)
PHA/DDGS
PLA/Soy/BR (55/35/10)
rPLA 2.2 A 3.8 A 3.8 A 3.9 A 3.9 A
PP container 1.8 A 3.8 A 3.9 A 3.9 A 3.9 A
Container Nutrition Trial
Fertilizer Treatment (mg of N)
0 60 113 226 452
Petroleum Plastic
PLA/Soy/BR (50/30/20)
PLA/Soy (60/40)
PLA/Soy/BR (55/35/10)
Container Nutrition Trial
Average Root Circling Rating (1=no root circling, 4=complete root circling)
Fertilizer Treatment (mg of N)
Container Type 0 60 113 226 452
PLA/BR (80/20)
PLA/Lignin
PLA/Soy/BR (50/30/20) 1.1 A 1.4 C 1.2 D 1.4 D 1.2 D
PLA/Soy (60/40) 1.7 A 1.8 BC 2.0 C 2.2 C 1.8 C
PHA/DDGS
PLA/Soy/BR (55/35/10) 1.3 A 1.8 BC 1.8 C 1.8 CD 1.7 C
rPLA
PP container 1.8 A 3.8 A 3.9 A 3.9 A 3.9 A
Container Nutrition Trial
• Soy-based biocontainers can be used up to 10 weeks, especially when
grown in drier conditions
• Shuttle trays can facilitate microbial growth on the outside of
containers made with more degradable materials
• Biocontainers made from more durable materials can last much
longer than 10 weeks
• Soy-based biocontainer release mineral nutrients and can reduce
fertilizer applications
• Root pruning occurred on plants grown in soy-based biocontainers
and PHA/DDGS biocontainers
Take-Home Messages
Acknowledgements
Dr. Christopher Currey and Dr. William Graves, James Schrader,
and Peter Lawlor
ISU Department of Horticulture and College of Agriculture and
Life Sciences
Personnel at the Center for Crops Utilization and Research
USDA Specialty Crop Research Initiative
Questions?
Shoot Phosphorus (P) characteristics
Fertilizer Applied (mg of N)
Container Type 0 60 113 226 452
Shoot P (%)
PLA/BR (80/20) 0.10 Cd 0.18 Ccd 0.25 CDc 0.37 Ab 0.56 Aa
PLA/Lignin 0.09 Cd 0.18 Ccd 0.24 CDc 0.46 Ab 0.56 Aa
PLA/BR/Soy (50/30/20) 0.28 Bb 0.31 ABb 0.42 ABb 0.47 Aab 0.64 Aa
PLA/Soy (60/40) 0.31 Abc 0.35 Abc 0.44 Aabc 0.50 Aab 0.54 Aa
PHA/DDGS 0.25 Bc 0.25 BCc 0.32 A-Dc 0.40 Ab 0.58 Aa
PLA/BR/Soy (55/35/10) 0.36 Ac 0.35 Ac 0.40 A-Cbc 0.49 Ab 0.62 Aa
rPLA 0.06 Cc 0.18 Cbc 0.22 Db 0.45 Aa 0.57 Aa
PP container 0.07 Cd 0.19 Ccd 0.26 B-Dbc 0.37 Ab 0.53 Aa
Shoot P content (mg)
PLA/BR (80/20) 0.7 Be 5.4 Bd 7.8 Bc 18.5 Bb 33.6 ABa
PLA/Lignin 0.7 Be 5.3 Bd 9.4 Bc 20.6 Bb 34.1 ABa
PLA/BR/Soy (50/30/20) 7.0 Ac 14.3 Ab 17.0 Ab 26.4 Aa 29.7 Ba
PLA/Soy (60/40) 7.5 Ae 11.9 Ad 16.7 Ac 25.9 Ab 39.7 Aa
PHA/DDGS 1.2 Be 6.0 Bd 9.0 Bc 18.9 Bb 31.5 Ba
PLA/BR/Soy (55/35/10) 6.2 Ad 12.2 Ac 15.9 Ac 26.6 Ab 35.9 ABa
rPLA 0.3 Bd 5.5 Bc 7.8 Bc 19.2 Bb 32.9 Ba
PP container 0.5 Bd 5.8 Bc 9.1 Bc 18.5 Bb 35.5 ABa
Shoot Potassium (K) characteristics
Fertilizer Applied (mg of N)
Container Type 0 60 113 226 452
Shoot K (%)
PLA/BR (80/20) 1.99 Cb 1.68 BCb 1.93 B-Db 2.30 ABab 2.84 ABa
PLA/Lignin 1.55 Cb 1.36 Cb 1.70 Db 2.54 ABa 2.84 ABa
PLA/BR/Soy (50/30/20) 2.30 BCb 2.19 ABb 2.86 ABab 2.73 ABab 3.44 Aa
PLA/Soy (60/40) 2.97 ABa 2.68 Aa 3.21 Aa 3.35 Aa 2.88 ABa
PHA/DDGS 1.81 Cb 1.74 BCb 2.29 A-Db 2.36 ABb 3.42 ABa
PLA/BR/Soy (55/35/10) 3.25 Aa 2.82 Aa 2.81 ABCa 2.93 ABa 3.20 ABa
rPLA 1.75 Cb 1.43 Cb 1.66 Db 2.75 ABa 2.78 ABa
PP container 1.54 Cb 1.53 BCb 1.84 CDab 1.97 Bab 2.48 Ba
Shoot K content (mg)
PLA/BR (80/20) 14.7 Cd 51.2 Bc 61.0 Bc 115.8 Bb 170.6 Aa
PLA/Lignin 12.5 Ce 40.7 Bd 65.8 Bc 113.4 Bb 173.5 Aa
PLA/BR/Soy (50/30/20) 59.5 ABd 100.8 Acd 117.3 Abc 153.9 Aab 163.5 Aa
PLA/Soy (60/40) 69.8 Ad 90.7 Ad 122.0 Ac 173.7 Ab 211.4 Aa
PHA/DDGS 8.8 Cd 41.5 Bc 64.5 Bc 111.2 Bb 185.5 Aa
PLA/BR/Soy (55/35/10) 55.7 Bc 97.9 Ab 112.5 Ab 160.1 Aa 184.5 Aa
rPLA 7.2 Cd 44.5 Bc 58.7 Bc 115.8 Bb 163.5 Aa
PP container 10.4 Cd 46.4 Bc 63.6 Bc 100.2 Bb 165.5 Aa

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Greenhouse Performance of Round 3 Containers

  • 1. Greenhouse Performance of Round 3 Containers Kenneth G McCabe Department of Horticulture Iowa State University
  • 2. Outline for Presentation • Container Aesthetics and Shuttle Tray Experiments • Greenhouse Nutrition Trial • Take-home Messages • Acknowledgements and Questions
  • 3. Container Aesthetics Experiment Gerbera (10 weeks) Asparagus Fern (15 weeks)
  • 5. Containers used for 10 weeks PLA/Soy (60/40) PLA/Soy/BioRes (50/30/20) PLA/Soy/BioRes (55/35/10) PLA/BioRes (BR) (80/20) PLA/Lignin (90/10) rPLA (100) PHA/DDGS (80/20) Petroleum plastic PUR-coated Paper-fiber
  • 6. Asparagus Fern 15 weeks old PLA/BioRes containers with and without colorant Blue Colorant Green Colorant No Color Added
  • 7. Shuttle Tray Experiment Marigold ‘Honeycomb’ grown for 7 weeks Bioplastic Containers Only
  • 18. Container Nutrition Trial Marigold ‘Honeycomb’ 5 weeks of culture 5 Fertilizer Treatments
  • 19. 4.5” Containers Evaluated (% by weight) PLA/BioRes (BR) (80/20) PLA/Lignin (90/10) PLA/Soy/BR (50/30/20) PLA/Soy (60/40) PHA/DDGS (80/20) PLA/Soy/BR (55/35/10) rPLA (100) Petroleum Plastic - polypropylene
  • 20. Fertilizer Treatments Wk 1 Wk 2 Wk 3 Wk 4 Wk 5 (0) (60) (113) (226) (452) 0 0 0 0 0 100 100 0 0 0 75 75 75 75 75 150 150 150 150 150 300 300 300 300 300 Fertilizer (ppm N) supplied from a 16.6N–5P–16.3K water-soluble fertilizer Applied N (mg) 1) 2) 3) 4) 5)
  • 21. Petroleum Plastic PLA/Soy/BR (50/30/20) PLA/Soy (60/40) PLA/Soy/BR (55/35/10) rPLA (100%) PHA/DDGS (80/20) PLA/Lignin (90/10) PLA/BR (80/20) Fertilizer Trt (mg of N) 0 6 113 226 452Container Nutrition Trial
  • 22. Shoot Dry Weight (g) Fertilizer Treatment (mg of N) Container Type 0 60 113 226 452 PLA/BR (80/20) 0.8 Cd 3.2 BCc 3.4 ABc 4.7 Ab 6.1 Aa PLA/Lignin 0.8 Cd 2.9 BCc 3.9 ABb 5.1 Aa 5.7 Aa PLA/Soy/BR (50/30/20) 2.6 Ac 4.6 Aab 4.1 Ab 5.9 Aa 5.4 Aab PLA/Soy (60/40) 2.6 Ad 3.6 Bcd 4.5 Abc 5.3 Aab 6.3 Aa PHA/DDGS 0.5 Cc 2.4 Cb 2.9 Bb 4.9 Aa 5.5 Aa PLA/Soy/BR (55/35/10) 1.8 Bd 3.8 ABc 4.2 Abc 5.5 Aab 5.9 Aa rPLA 0.4 Cd 3.1 BCc 3.6 ABc 4.9 Ab 6.3 Aa PP container 0.7 Cd 3.0 BCc 3.8 ABbc 4.8 Ab 6.7 Aa Container Nutrition Trial
  • 23. Petroleum Plastic PLA/Soy/BR (50/30/20) PLA/Soy (60/40) PLA/Soy/BR (55/35/10) Fertilizer Treatment (mg of N) 0 60 113 226 452Container Nutrition Trial
  • 24. Petroleum Plastic PLA/Soy/BR (50/30/20) PLA/Soy (60/40) PLA/Soy/BR (55/35/10) Fertilizer Treatment (mg of N) 0 60 113 226 452Container Nutrition Trial
  • 25. Shoot Dry Weight (g) Fertilizer Treatment (mg of N) Container Type 0 60 113 226 452 PLA/BR (80/20) PLA/Lignin PLA/Soy/BR (50/30/20) 2.6 Ac 4.6 Aab 4.1 Ab 5.9 Aa 5.4 Aab PLA/Soy (60/40) 2.6 Ad 3.6 Bcd 4.5 Abc 5.3 Aab 6.3 Aa PHA/DDGS PLA/Soy/BR (55/35/10) 1.8 Bd 3.8 ABc 4.2 Abc 5.5 Aab 5.9 Aa rPLA PP container 0.7 Cd 3.0 BCc 3.8 ABbc 4.8 Ab 6.7 Aa Container Nutrition Trial
  • 26. Petroleum Plastic PLA/BR (80/20) PLA/Lignin (90/10) rPLA (100) Fertilizer Treatment (mg of N) 0 60 113 226 452 Container Nutrition Trial
  • 27. Shoot Dry Weight (g) Fertilizer Treatment (mg of N) Container Type 0 60 113 226 452 PLA/BR (80/20) 0.8 Cd 3.2 BCc 3.4 ABc 4.7 Ab 6.1 Aa PLA/Lignin 0.8 Cd 2.9 BCc 3.9 ABb 5.1 Aa 5.7 Aa PLA/Soy/BR (50/30/20) PLA/Soy (60/40) PHA/DDGS PLA/Soy/BR (55/35/10) rPLA 0.4 Cd 3.1 BCc 3.6 ABc 4.9 Ab 6.3 Aa PP container 0.7 Cd 3.0 BCc 3.8 ABbc 4.8 Ab 6.7 Aa Container Nutrition Trial
  • 28. Petroleum Plastic PHA/DDGS (80/20) Fertilizer Treatment (mg of N) 0 60 113 226 452 Container Nutrition Trial
  • 29. Shoot Dry Weight (g) Fertilizer Treatment (mg of N) Container Type 0 60 113 226 452 PLA/BR (80/20) PLA/Lignin PLA/Soy/BR (50/30/20) PLA/Soy (60/40) PHA/DDGS 0.5 Cc 2.4 Cb 2.9 Bb 4.9 Aa 5.5 Aa PLA/Soy/BR (55/35/10) rPLA PP container 0.7 Cd 3.0 BCc 3.8 ABbc 4.8 Ab 6.7 Aa Container Nutrition Trial
  • 30. Petroleum Plastic PLA/Soy/BR (50/30/20) PLA/Soy (60/40) PLA/Soy/BR (55/35/10) rPLA (100%) PHA/DDGS (80/20) PLA/Lignin (90/10) PLA/BR (80/20) Fertilizer Trt (mg of N) 0 6 113 226 452Container Nutrition Trial
  • 31. Shoot Nitrogen (N) characteristics Fertilizer Treatment (mg of N) Container Type 0 60 113 226 452 Shoot N (%) PLA/BR (80/20) 1.2 Cc 1.4 Cc 2.3 Cb 2.3 CDb 3.4 BCa PLA/Lignin 0.7 Cd 1.3 Cc 2.1 Cb 3.2 BCa 3.5 BCa PLA/Soy/BR (50/30/20) 4.5 Aa 4.0 Aa 4.6 Aa 4.1 Aa 4.4 Aa PLA/Soy (60/40) 2.7 Ba 2.8 Ba 3.3 Ba 3.1 BCa 3.6 A-Ca PHA/DDGS 0.7 Cc 1.0 Cc 1.6 Cb 1.9 Db 3.0 Ca PLA/Soy/BR (55/35/10) 4.1 Aa 3.0 Bb 3.7 Bab 3.5 ABab 4.1 ABa rPLA 0.7 Cd 1.3 Ccd 1.9 Cc 2.7 B-Db 3.5 BCa PP container 0.8 Cc 1.3 Cc 2.2 Cb 2.5 CDb 3.3 Ca Shoot N content (mg) PLA/BR (80/20) 9 Ce 43 Cd 71 Cc 117 DEb 207 ABa PLA/Lignin 6 Ce 38 Cd 80 Cc 139 CDb 216 ABa PLA/Soy/BR (50/30/20) 120 Ab 181 Aab 199 Aab 231 Aa 220 ABa PLA/Soy (60/40) 66 Bd 93 Bd 129 Bc 163 BCb 264 Aa PHA/DDGS 4 Ce 25 Cd 46 Cc 87 Eb 163 Ba PLA/Soy/BR (55/35/10) 72 Bc 109 Bbc 146 Bb 194 Ba 236 ABa rPLA 3 Cd 40 Cc 65 Cc 117 DEb 205 ABa PP container 5 Ce 40 Cd 74 Cc 128 Db 221 ABa
  • 32. Petroleum Plastic PLA/Soy/BR (50/30/20) PLA/Soy (60/40) PLA/Soy/BR (55/35/10) Fertilizer Treatment (mg of N) 0 60 113 226 452 Container Nutrition Trial
  • 33. Shoot Nitrogen (N) characteristics Fertilizer Treatment (mg of N) Container Type 0 60 113 226 452 Shoot N (%) PLA/BR (80/20) PLA/Lignin PLA/Soy/BR (50/30/20) 4.5 Aa 4.0 Aa 4.6 Aa 4.1 Aa 4.4 Aa PLA/Soy (60/40) 2.7 Ba 2.8 Ba 3.3 Ba 3.1 BCa 3.6 A-Ca PHA/DDGS PLA/Soy/BR (55/35/10) 4.1 Aa 3.0 Bb 3.7 Bab 3.5 ABab 4.1 ABa rPLA PP container 0.8 Cc 1.3 Cc 2.2 Cb 2.5 CDb 3.3 Ca Shoot N content (mg) PLA/BR (80/20) PLA/Lignin PLA/Soy/BR (50/30/20) 120 Ab 181 Aab 199 Aab 231 Aa 220 ABa PLA/Soy (60/40) 66 Bd 93 Bd 129 Bc 163 BCb 264 Aa PHA/DDGS PLA/Soy/BR (55/35/10) 72 Bc 109 Bbc 146 Bb 194 Ba 236 ABa rPLA PP container 5 Ce 40 Cd 74 Cc 128 Db 221 ABa
  • 34. Petroleum Plastic PLA/Soy/BR (50/30/20) PLA/Soy (60/40) PLA/Soy/BR (55/35/10) Fertilizer Treatment (mg of N) 0 60 113 226 452 Container Nutrition Trial
  • 35. Shoot Nitrogen (N) characteristics Fertilizer Treatment (mg of N) Container Type 0 60 113 226 452 Shoot N (%) PLA/BR (80/20) PLA/Lignin PLA/Soy/BR (50/30/20) 4.5 Aa 4.0 Aa 4.6 Aa 4.1 Aa 4.4 Aa PLA/Soy (60/40) 2.7 Ba 2.8 Ba 3.3 Ba 3.1 BCa 3.6 A-Ca PHA/DDGS PLA/Soy/BR (55/35/10) 4.1 Aa 3.0 Bb 3.7 Bab 3.5 ABab 4.1 ABa rPLA PP container 0.8 Cc 1.3 Cc 2.2 Cb 2.5 CDb 3.3 Ca Shoot N content (mg) PLA/BR (80/20) PLA/Lignin PLA/Soy/BR (50/30/20) 120 Ab 181 Aab 199 Aab 231 Aa 220 ABa PLA/Soy (60/40) 66 Bd 93 Bd 129 Bc 163 BCb 264 Aa PHA/DDGS PLA/Soy/BR (55/35/10) 72 Bc 109 Bbc 146 Bb 194 Ba 236 ABa rPLA PP container 5 Ce 40 Cd 74 Cc 128 Db 221 ABa
  • 36. Shoot Nitrogen (N) characteristics Fertilizer Treatment (mg of N) Container Type 0 60 113 226 452 Shoot N (%) PLA/BR (80/20) PLA/Lignin PLA/Soy/BR (50/30/20) 4.5 Aa 4.0 Aa 4.6 Aa 4.1 Aa 4.4 Aa PLA/Soy (60/40) 2.7 Ba 2.8 Ba 3.3 Ba 3.1 BCa 3.6 A-Ca PHA/DDGS PLA/Soy/BR (55/35/10) 4.1 Aa 3.0 Bb 3.7 Bab 3.5 ABab 4.1 ABa rPLA PP container 0.8 Cc 1.3 Cc 2.2 Cb 2.5 CDb 3.3 Ca Shoot N content (mg) PLA/BR (80/20) PLA/Lignin PLA/Soy/BR (50/30/20) 120 Ab 181 Aab 199 Aab 231 Aa 220 ABa PLA/Soy (60/40) 66 Bd 93 Bd 129 Bc 163 BCb 264 Aa PHA/DDGS PLA/Soy/BR (55/35/10) 72 Bc 109 Bbc 146 Bb 194 Ba 236 ABa rPLA PP container 5 Ce 40 Cd 74 Cc 128 Db 221 ABa
  • 37. Petroleum Plastic PLA/Soy/BR (50/30/20) PLA/Soy (60/40) PLA/Soy/BR (55/35/10) Fertilizer Treatment (mg of N) 0 60 113 226 452 Container Nutrition Trial
  • 38. Shoot Nitrogen (N) characteristics Fertilizer Treatment (mg of N) Container Type 0 60 113 226 452 Shoot N (%) PLA/BR (80/20) PLA/Lignin PLA/Soy/BR (50/30/20) 4.5 Aa 4.0 Aa 4.6 Aa 4.1 Aa 4.4 Aa PLA/Soy (60/40) 2.7 Ba 2.8 Ba 3.3 Ba 3.1 BCa 3.6 A-Ca PHA/DDGS PLA/Soy/BR (55/35/10) 4.1 Aa 3.0 Bb 3.7 Bab 3.5 ABab 4.1 ABa rPLA PP container 0.8 Cc 1.3 Cc 2.2 Cb 2.5 CDb 3.3 Ca Shoot N content (mg) PLA/BR (80/20) PLA/Lignin PLA/Soy/BR (50/30/20) 120 Ab 181 Aab 199 Aab 231 Aa 220 ABa PLA/Soy (60/40) 66 Bd 93 Bd 129 Bc 163 BCb 264 Aa PHA/DDGS PLA/Soy/BR (55/35/10) 72 Bc 109 Bbc 146 Bb 194 Ba 236 ABa rPLA PP container 5 Ce 40 Cd 74 Cc 128 Db 221 ABa
  • 39. Shoot Nitrogen (N) characteristics Fertilizer Treatment (mg of N) Container Type 0 60 113 226 452 Shoot N (%) PLA/BR (80/20) PLA/Lignin PLA/Soy/BR (50/30/20) 4.5 Aa 4.0 Aa 4.6 Aa 4.1 Aa 4.4 Aa PLA/Soy (60/40) 2.7 Ba 2.8 Ba 3.3 Ba 3.1 BCa 3.6 A-Ca PHA/DDGS PLA/Soy/BR (55/35/10) 4.1 Aa 3.0 Bb 3.7 Bab 3.5 ABab 4.1 ABa rPLA PP container 0.8 Cc 1.3 Cc 2.2 Cb 2.5 CDb 3.3 Ca Shoot N content (mg) PLA/BR (80/20) PLA/Lignin PLA/Soy/BR (50/30/20) 120 Ab 181 Aab 199 Aab 231 Aa 220 ABa PLA/Soy (60/40) 66 Bd 93 Bd 129 Bc 163 BCb 264 Aa PHA/DDGS PLA/Soy/BR (55/35/10) 72 Bc 109 Bbc 146 Bb 194 Ba 236 ABa rPLA PP container 5 Ce 40 Cd 74 Cc 128 Db 221 ABa
  • 40. Petroleum Plastic PLA/BR (80/20) PLA/Lignin (90/10) rPLA (100) Fertilizer Treatment (mg of N) 0 60 113 226 452 Container Nutrition Trial
  • 41. Shoot Nitrogen (N) characteristics Fertilizer Treatment (mg of N) Container Type 0 60 113 226 452 Shoot N (%) PLA/BR (80/20) 1.2 C 1.4 C 2.3 C 2.3 CD 3.4 BC PLA/Lignin 0.7 C 1.3 C 2.1 C 3.2 BC 3.5 BC PLA/Soy/BR (50/30/20) PLA/Soy (60/40) PHA/DDGS PLA/Soy/BR (55/35/10) rPLA 0.7 C 1.3 C 1.9 C 2.7 B-D 3.5 BC PP container 0.8 C 1.3 C 2.2 C 2.5 CD 3.3 C Shoot N content (mg) PLA/BR (80/20) 9 Ce 43 Cd 71 Cc 117 DEb 207 ABa PLA/Lignin 6 Ce 38 Cd 80 Cc 139 CDb 216 ABa PLA/Soy/BR (50/30/20) PLA/Soy (60/40) PHA/DDGS PLA/Soy/BR (55/35/10) rPLA 3 Cd 40 Cc 65 Cc 117 DEb 205 ABa PP container 5 Ce 40 Cd 74 Cc 128 Db 221 ABa
  • 42. Petroleum Plastic PHA/DDGS (80/20) Fertilizer Treatment (mg of N) 0 60 113 226 452 Container Nutrition Trial
  • 43. Shoot Nitrogen (N) characteristics Fertilizer Treatment (mg of N) Container Type 0 60 113 226 452 Shoot N (%) PLA/BR (80/20) PLA/Lignin PLA/Soy/BR (50/30/20) PLA/Soy (60/40) PHA/DDGS 0.7 C 1.0 C 1.6 C 1.9 D 3.0 C PLA/Soy/BR (55/35/10) rPLA PP container 0.8 C 1.3 C 2.2 C 2.5 CD 3.3 C Shoot N content (mg) PLA/BR (80/20) PLA/Lignin PLA/Soy/BR (50/30/20) PLA/Soy (60/40) PHA/DDGS 4 C 25 C 46 C 87 E 163 B PLA/Soy/BR (55/35/10) rPLA PP container 5 C 40 C 74 C 128 D 221 AB
  • 44. 100 ppm N for the first 2 weeks only (60 mg N) Petroleumplastic PLA/Soy/BR (50/30/20)
  • 45. Petroleum Plastic PLA/Soy/BR (50/30/20) PLA/Soy (60/40) PLA/Soy/BR (55/35/10) rPLA (100%) PHA/DDGS (80/20) PLA/Lignin (90/10) PLA/BioRes (80/20) Fertilizer Treatment (mg of N) 0 60 113 226 452 Container Nutrition Trial
  • 46. Average Root Circling Rating (1=no root circling, 4=complete root circling) Fertilizer Treatment (mg of N) Container Type 0 60 113 226 452 PLA/BR (80/20) 2.1 Ab 3.6 Aa 3.8 Aa 3.9 Aa 3.5 Aa PLA/Lignin 1.9 Ab 3.6 Aa 3.9 Aa 3.9 Aa 3.9 Aa PLA/Soy/BR (50/30/20) 1.1 Ab 1.4 Ca 1.2 Dab 1.4 Da 1.2 Dab PLA/Soy (60/40) 1.7 Ab 1.8 BCa 2.0 Cab 2.2 Ca 1.8 Cab PHA/DDGS 1.4 Ac 2.2 Bb 2.6 Bab 3.1 Ba 2.6 Bab PLA/Soy/BR (55/35/10) 1.3 Ab 1.8 BCa 1.8 Ca 1.8 CDa 1.7 Cab rPLA 2.2 Ab 3.8 Aa 3.8 Aa 3.9 Aa 3.9 Aa PP container 1.8 Ab 3.8 Aa 3.9 Aa 3.9 Aa 3.9 Aa Container Nutrition Trial
  • 47. Petroleum Plastic PLA/BR (80/20) PLA/Lignin (90/10) rPLA (100) Fertilizer Treatment (mg of N) 0 60 113 226 452 Container Nutrition Trial
  • 48. Average Root Circling Rating (1=no root circling, 4=complete root circling) Fertilizer Treatment (mg of N) Container Type 0 60 113 226 452 PLA/BR (80/20) 2.1 A 3.6 A 3.8 A 3.9 A 3.5 A PLA/Lignin 1.9 A 3.6 A 3.9 A 3.9 A 3.9 A PLA/Soy/BR (50/30/20) PLA/Soy (60/40) PHA/DDGS PLA/Soy/BR (55/35/10) rPLA 2.2 A 3.8 A 3.8 A 3.9 A 3.9 A PP container 1.8 A 3.8 A 3.9 A 3.9 A 3.9 A Container Nutrition Trial
  • 49. Fertilizer Treatment (mg of N) 0 60 113 226 452 Petroleum Plastic PLA/Soy/BR (50/30/20) PLA/Soy (60/40) PLA/Soy/BR (55/35/10) Container Nutrition Trial
  • 50. Average Root Circling Rating (1=no root circling, 4=complete root circling) Fertilizer Treatment (mg of N) Container Type 0 60 113 226 452 PLA/BR (80/20) PLA/Lignin PLA/Soy/BR (50/30/20) 1.1 A 1.4 C 1.2 D 1.4 D 1.2 D PLA/Soy (60/40) 1.7 A 1.8 BC 2.0 C 2.2 C 1.8 C PHA/DDGS PLA/Soy/BR (55/35/10) 1.3 A 1.8 BC 1.8 C 1.8 CD 1.7 C rPLA PP container 1.8 A 3.8 A 3.9 A 3.9 A 3.9 A Container Nutrition Trial
  • 51. • Soy-based biocontainers can be used up to 10 weeks, especially when grown in drier conditions • Shuttle trays can facilitate microbial growth on the outside of containers made with more degradable materials • Biocontainers made from more durable materials can last much longer than 10 weeks • Soy-based biocontainer release mineral nutrients and can reduce fertilizer applications • Root pruning occurred on plants grown in soy-based biocontainers and PHA/DDGS biocontainers Take-Home Messages
  • 52. Acknowledgements Dr. Christopher Currey and Dr. William Graves, James Schrader, and Peter Lawlor ISU Department of Horticulture and College of Agriculture and Life Sciences Personnel at the Center for Crops Utilization and Research USDA Specialty Crop Research Initiative
  • 54. Shoot Phosphorus (P) characteristics Fertilizer Applied (mg of N) Container Type 0 60 113 226 452 Shoot P (%) PLA/BR (80/20) 0.10 Cd 0.18 Ccd 0.25 CDc 0.37 Ab 0.56 Aa PLA/Lignin 0.09 Cd 0.18 Ccd 0.24 CDc 0.46 Ab 0.56 Aa PLA/BR/Soy (50/30/20) 0.28 Bb 0.31 ABb 0.42 ABb 0.47 Aab 0.64 Aa PLA/Soy (60/40) 0.31 Abc 0.35 Abc 0.44 Aabc 0.50 Aab 0.54 Aa PHA/DDGS 0.25 Bc 0.25 BCc 0.32 A-Dc 0.40 Ab 0.58 Aa PLA/BR/Soy (55/35/10) 0.36 Ac 0.35 Ac 0.40 A-Cbc 0.49 Ab 0.62 Aa rPLA 0.06 Cc 0.18 Cbc 0.22 Db 0.45 Aa 0.57 Aa PP container 0.07 Cd 0.19 Ccd 0.26 B-Dbc 0.37 Ab 0.53 Aa Shoot P content (mg) PLA/BR (80/20) 0.7 Be 5.4 Bd 7.8 Bc 18.5 Bb 33.6 ABa PLA/Lignin 0.7 Be 5.3 Bd 9.4 Bc 20.6 Bb 34.1 ABa PLA/BR/Soy (50/30/20) 7.0 Ac 14.3 Ab 17.0 Ab 26.4 Aa 29.7 Ba PLA/Soy (60/40) 7.5 Ae 11.9 Ad 16.7 Ac 25.9 Ab 39.7 Aa PHA/DDGS 1.2 Be 6.0 Bd 9.0 Bc 18.9 Bb 31.5 Ba PLA/BR/Soy (55/35/10) 6.2 Ad 12.2 Ac 15.9 Ac 26.6 Ab 35.9 ABa rPLA 0.3 Bd 5.5 Bc 7.8 Bc 19.2 Bb 32.9 Ba PP container 0.5 Bd 5.8 Bc 9.1 Bc 18.5 Bb 35.5 ABa
  • 55. Shoot Potassium (K) characteristics Fertilizer Applied (mg of N) Container Type 0 60 113 226 452 Shoot K (%) PLA/BR (80/20) 1.99 Cb 1.68 BCb 1.93 B-Db 2.30 ABab 2.84 ABa PLA/Lignin 1.55 Cb 1.36 Cb 1.70 Db 2.54 ABa 2.84 ABa PLA/BR/Soy (50/30/20) 2.30 BCb 2.19 ABb 2.86 ABab 2.73 ABab 3.44 Aa PLA/Soy (60/40) 2.97 ABa 2.68 Aa 3.21 Aa 3.35 Aa 2.88 ABa PHA/DDGS 1.81 Cb 1.74 BCb 2.29 A-Db 2.36 ABb 3.42 ABa PLA/BR/Soy (55/35/10) 3.25 Aa 2.82 Aa 2.81 ABCa 2.93 ABa 3.20 ABa rPLA 1.75 Cb 1.43 Cb 1.66 Db 2.75 ABa 2.78 ABa PP container 1.54 Cb 1.53 BCb 1.84 CDab 1.97 Bab 2.48 Ba Shoot K content (mg) PLA/BR (80/20) 14.7 Cd 51.2 Bc 61.0 Bc 115.8 Bb 170.6 Aa PLA/Lignin 12.5 Ce 40.7 Bd 65.8 Bc 113.4 Bb 173.5 Aa PLA/BR/Soy (50/30/20) 59.5 ABd 100.8 Acd 117.3 Abc 153.9 Aab 163.5 Aa PLA/Soy (60/40) 69.8 Ad 90.7 Ad 122.0 Ac 173.7 Ab 211.4 Aa PHA/DDGS 8.8 Cd 41.5 Bc 64.5 Bc 111.2 Bb 185.5 Aa PLA/BR/Soy (55/35/10) 55.7 Bc 97.9 Ab 112.5 Ab 160.1 Aa 184.5 Aa rPLA 7.2 Cd 44.5 Bc 58.7 Bc 115.8 Bb 163.5 Aa PP container 10.4 Cd 46.4 Bc 63.6 Bc 100.2 Bb 165.5 Aa