Supercritical Fluid Chromatography
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Reviews the SFC technique along with applications and case studies. Use of animations disturbs the view. For full ppt, drop me a mail at varadbende96@gmail.com
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Supercritical Fluid Chromatography
- 1. By Guided by Varad P. Bende Dr. S. D. Saptarshi Department of Biotechnology MGM’s Jawaharlal Nehru Engineering College, Aurangabad. MS.
- 2. Contents • Supercritical Fluids • Chromatography • Supercritical Fluid Chromatography (SFC) • Instrumentation of SFC • Advantages and Challenges • Applications of SFC • Case Studies
- 4. • Critical Pressure (PC) • Critical Temperature (TC) Supercritical
- 5. Liquid Gas Density (g/cm3) Diffusivity (cm2/s) Viscosity (Pa . s) Gas 10-5 10-1 10-4 Supercritical fluids 10-2 - 1 10-4 - 10-3 10-4 - 10-5 Liquid 1 10-5 10-2 SCF
- 6. Effect of Pressure and Temperature
- 9. Stationary Phase Mobile Phase Chromatographic Technique CHROMATOGRAPHY Gas Liquid Gas Liquid GLC Solid Gas Solid GSC Liquid Liquid Paper Chromtography Solid TLC Liquid Solid LC Bonded Phase Size Exclusion Ion exchange AffinitySupercritical fluid Liquid Supercritical Fluid ChromatographySolid Classification of Chromatography
- 13. Supercritical Fluids Properties of Gases High Diffusivity Low Viscosity Properties of Liuquid Density Solvating Power •Dissolution •Displacement of . matrix •Transport •Penetrability
- 14. Mobile phase - CO2 •Chemically inert •Cheap •Non toxic •Non flammable •Low critical temperature and pressure
- 16. INSTRUMENTATION
- 18. Advantages •Cost of operation •Non toxic •Low back pressure •High resolution •Vast range of analytes •Simple configuration
- 19. Challenges High capital cost Cannot analyse extremely polar analytes Detector incompatibility Use of organic solvents as modifiers
- 20. APPLICATIONS •Enantioseparations •Pharma industry •Chiral compounds •Polypeptides •Lipophilic compounds •Pigments •Biologically active compounds
- 21. Manufacturers JASCO SHIMADZU Waters Agilent Technologies
- 22. CASE STUDIES
- 23. Purification of Vitamin E •Analyte of Interest - α-tocopherol from γ- and δ -tocopherol •Mobile phase - CO 2 + 3.98% EtOH •Stationary phase - Silica Chongwen Jiang , et al. (2003) •Temperature - 40 °C Pressure-18 MPa
- 24. Purification of Polypeptide •Analyte of Interest - Isomeric polypeptides (547/541 and 541/544) •Mobile phase - CO 2 + 0.2% TFA (in MetOH) •Stationary phase - Pyridine capped silica M A Patel , et al. (2011) •Temperature - 40 °C Pressure-10 MPa
- 25. Purification of Cyclosporins •Analyte of Interest - Cyclosporin analogs A,B,C,D,H •Mobile phase - CO 2 + EtOH •Stationary phase - Silica Yuefi Shao , et al. (2016) •Temperature - 80 °C Pressure-15 MPa
- 26. Purification of Carotenoids •Analyte of Interest - Carotenoids and isomers •Mobile phase - CO 2 + 1 %EtOH •Stationary phase - Phenyl Polymethylsiloxane H H Schmitz , et al. (1989) •Temperature - 45 °C
- 27. Purification of Polyunsaturated Fatty Acids •Analyte of Interest - EPA and DHA •Mobile phase - CO 2 •Stationary phase - C18 M Alkioa , et al. (2000) •Temperature - 65 °C Pressure-14.5 MPa