Temporary Labs, Community Involvement and BioLabs discusses how to start community bio labs through sharing experiences setting up various labs over time. It outlines how Dr. Marc Dusseiller was involved in labs since 2006 and ran nanolab courses from 2008-2012. It emphasizes that people are more important than infrastructure and shares how the BioTehna lab in Ljubljana was started in 2012 and has expanded through collaborative workshops and growing community involvement. The document promotes starting labs anywhere through flexible do-it-yourself approaches and networking with others.
This document discusses the development of DIY scientific instruments and open source laboratory infrastructure. It provides examples of citizen science projects involving DIY microscopy, turbidity meters, incubators, and other lab devices built using inexpensive and accessible materials like Arduinos. The overall goal is to demystify science and make laboratory tools more available to amateur scientists, students, and communities through open source sharing of designs, instructions, and a global network of knowledge.
This document discusses making and generic lab equipment. It provides contact information for Urs Gaudenz and notes that he works in open source biological art, DIY biology, and generic lab equipment. It discusses using a kitchen as a bio lab and emphasizes sharing knowledge openly through community collaboration and mobile labs. It provides examples of homemade lab equipment and links to instructions for a webcam microscope, microcentrifuge, and spectrophotometer. It stresses that biology takes time and is about living things.
Open Source Generic Lab Equipement - GaudiLabsUrs Gaudenz
How to build you own BioLab from recycled materials. Examples of generic lab equipment and what they are made of. Overview of a set of mobile devices. More advanced scientific lab instruments like the laser tweezers (optical trap) and the OpenDrop Electro wetting platform for digital bio. A hackteria.org Project.
thGAP - BAbyss in Moderno!! Transgenic Human Germline Alternatives ProjectMarc Dusseiller Dusjagr
thGAP - Transgenic Human Germline Alternatives Project, presents an evening of input lectures, discussions and a performative workshop on artistic interventions for future scenarios of human genetic and inheritable modifications.
To begin our lecturers, Marc Dusseiller aka "dusjagr" and Rodrigo Martin Iglesias, will give an overview of their transdisciplinary practices, including the history of hackteria, a global network for sharing knowledge to involve artists in hands-on and Do-It-With-Others (DIWO) working with the lifesciences, and reflections on future scenarios from the 8-bit computer games of the 80ies to current real-world endeavous of genetically modifiying the human species.
We will then follow up with discussions and hands-on experiments on working with embryos, ovums, gametes, genetic materials from code to slime, in a creative and playful workshop setup, where all paticipant can collaborate on artistic interventions into the germline of a post-human future.
Temporary Labs, Community Involvement and BioLabs discusses how to start community bio labs through sharing experiences setting up various labs over time. It outlines how Dr. Marc Dusseiller was involved in labs since 2006 and ran nanolab courses from 2008-2012. It emphasizes that people are more important than infrastructure and shares how the BioTehna lab in Ljubljana was started in 2012 and has expanded through collaborative workshops and growing community involvement. The document promotes starting labs anywhere through flexible do-it-yourself approaches and networking with others.
This document discusses the development of DIY scientific instruments and open source laboratory infrastructure. It provides examples of citizen science projects involving DIY microscopy, turbidity meters, incubators, and other lab devices built using inexpensive and accessible materials like Arduinos. The overall goal is to demystify science and make laboratory tools more available to amateur scientists, students, and communities through open source sharing of designs, instructions, and a global network of knowledge.
This document discusses making and generic lab equipment. It provides contact information for Urs Gaudenz and notes that he works in open source biological art, DIY biology, and generic lab equipment. It discusses using a kitchen as a bio lab and emphasizes sharing knowledge openly through community collaboration and mobile labs. It provides examples of homemade lab equipment and links to instructions for a webcam microscope, microcentrifuge, and spectrophotometer. It stresses that biology takes time and is about living things.
Open Source Generic Lab Equipement - GaudiLabsUrs Gaudenz
How to build you own BioLab from recycled materials. Examples of generic lab equipment and what they are made of. Overview of a set of mobile devices. More advanced scientific lab instruments like the laser tweezers (optical trap) and the OpenDrop Electro wetting platform for digital bio. A hackteria.org Project.
thGAP - BAbyss in Moderno!! Transgenic Human Germline Alternatives ProjectMarc Dusseiller Dusjagr
thGAP - Transgenic Human Germline Alternatives Project, presents an evening of input lectures, discussions and a performative workshop on artistic interventions for future scenarios of human genetic and inheritable modifications.
To begin our lecturers, Marc Dusseiller aka "dusjagr" and Rodrigo Martin Iglesias, will give an overview of their transdisciplinary practices, including the history of hackteria, a global network for sharing knowledge to involve artists in hands-on and Do-It-With-Others (DIWO) working with the lifesciences, and reflections on future scenarios from the 8-bit computer games of the 80ies to current real-world endeavous of genetically modifiying the human species.
We will then follow up with discussions and hands-on experiments on working with embryos, ovums, gametes, genetic materials from code to slime, in a creative and playful workshop setup, where all paticipant can collaborate on artistic interventions into the germline of a post-human future.
dusjagr & nano talk on open tools for agriculture research and learningMarc Dusseiller Dusjagr
Open Tools for Research, Learning, Sharing in Agriculture and
Society as a whole.
Remote lecture by Marc Dusseiller and Fernando "nano" Castro on open science hardware for agriculture and edication, held for students of microbiology at UGM, Yogyakarta (remote - online )
UGM 2022: Open Source Biological Art and DIY / DIWO Scientific InstrumentsMarc Dusseiller Dusjagr
Overview of transdisciplinary approach bridging art and science in the global hackteria network.
Concrete examples of building low-cost scientific laboratory equiment.
Various DIY electronics for data logging.
what is art?
This document provides an overview of Dr. Marc R. Dusseiller's background and work investigating transdisciplinary approaches at the intersection of nanotechnology and human genome editing. It discusses his involvement with DIY biology and open source laboratory infrastructure projects since the 1980s aimed at making science more accessible. Specific projects mentioned include Hackteria workshops around the world to share skills for citizen science, as well as investigations into using nanoparticles for human genome editing and setting up a nano lab in a former bar in Slovenia.
Throughout the course, we will lift the fogs of these superficial discussions and through hands-on activities get closer to grasp the world of the small. We will build our own simple DIY (do-it-yourself) optical and electronic instruments to learn more about nanoparticles and nanosensors, how to “see” them, how to “hear” them.
During the 10 years of Hackteria.org we have established a global network and online knowledge base enabling practioners from diverse backgrounds to experiment with life science methodologies, from laboratory biology and genetics to environmental monitoring or fermentation. We have explored methods of collaboration, established in the early interenet culture, such as hackathons or docusprints as offline physical face-to-face production sessions, aswell as online tools for co-writing text-based instructions and lab notes. In this talk, I will give an overview of how our DIWO (Do-It-With-Others) method has lead to highly innovative projects, low-cost laboratory infrastructures, playful and critical prototypes, new workshop methods embracing a radically transdisciplinary approach to bridge the arts and the sciences.
Seoul 2019 - “Hackteria | Open Source Biological Art” - Transdisciplinary Ap...Marc Dusseiller Dusjagr
During the 10 years of Hackteria.org we have established a global network and online knowledge base enabling practioners from diverse backgrounds to experiment with life science methodologies, from laboratory biology and genetics to environmental monitoring or fermentation. We have explored methods of collaboration, established in the early interenet culture, such as hackathons or docusprints as offline physical face-to-face production sessions, aswell as online tools for co-writing text-based instructions and lab notes. In this talk, I will give an overview of how our DIWO (Do-It-With-Others) method has lead to highly innovative projects, low-cost laboratory infrastructures, playful and critical prototypes, new workshop methods embracing a radically transdisciplinary approach to bridge the arts and the sciences.
Presentation of the founding and background of Hackteria | Open Source Biological Art
Examples of modes of temporary collaborations during retreats, labs, camps and more.
WHAT IS/NOT WORKING
*/ Sesi konversasi mengenal kerja bersama
Organized along with Kunci and lifepatch, the event marks Hackteria’s tenth year as a global network that involves the collaboration of scientists, makers, hackers and artists in exploring new possibilities in bioart, open source hardware/software and experiments with biology, art/science based in various locations.
Instead of denoting what has been succeeded and how to pursue it, the conversation will explore possibilities of what could enable working together based on Kunci’s, Hackteria’s and lifepatch’s experience. The question ‘what is/not working’ may imply a ceased productivity, and at the same time, may provide opportunities to discover distinct values based on “non-productive” issues.
An overview of the 10 years history of Hackteria, establishing a global network of transdisciplinary collaborations and bioart workshops. Various examples of DIY science instruments, from rough educational prototypes to advanced open hardware for science.
This document provides an overview of the speaker's experiences with DIY biology and biohacking. It discusses the development of generic laboratory infrastructure to support citizen science, knowledge sharing and open source hardware. It describes various workshops and labs focused on transdisciplinary approaches at the intersection of biology, art, and design. Examples include DIY microscopy, synthetic biology competitions, environmental monitoring, and body hacking. The document emphasizes that biohacking has always existed as people find novel ways to apply science in their daily lives.
Creative PCB Design for Manufacturing using svg2shenzhen dusjagr taipeiMarc Dusseiller Dusjagr
I have explored many ways to make creative PCB designs for simple workshops in electronics and soldering, as part of the Swiss Mechatronic Art Society and in collaboration with other geeks worldwide. Pencil drawing, inkscape, homemade etching and in the end... creating Gerber files for Manufacturing using the amazing tool by Budi Prakosa "svg2shenzhen" to export from Inkscape to KiCad and produce nice gerber files. These slides are an overview of many examples over the years that lead to the most recent tool and PCBs.
This document provides an overview of DIY biology and biotechnology ("biohacking") through examples of workshops, labs, and projects. It discusses the global DIYbio community and efforts to provide open infrastructure like websites, instructions, and DIY lab instruments. Examples include homemade microscopy, PCR thermocyclers from hacked hairdryers, DNA testing, tissue engineering, fermentation, and mobile labs in Indonesia. The goal is democratizing access to tools and knowledge through a transdisciplinary approach involving artists, designers, researchers and citizens.
Overview of dusjagr's background from nanobiotechnology to making cheese and global workshopology. The role of temporary labs for collaborative prototyping, examples from Taiwan, Indonesia and Switzerland. Hackerspaces crossing digital- and biotechnologies.
This document discusses biohacking and open source biology. It begins by explaining that biohacking is about life and democratizing access to tools and knowledge. This includes topics like genetic engineering, DNA testing, and building one's own laboratory. The document then highlights several examples of open source biological art and DIY biology projects. It emphasizes building communities around collaborative development and workshopping. The goal is to make science more open and accessible to all.
- Dr. Marc Dusseiller discusses his experiences with biohacking and DIY (do-it-yourself) biology over the past 10+ years, including workshops held around the world.
- He helped establish hackerspaces and labs that merge digital and biological technologies to democratize science tools and knowledge.
- Examples include microfarming algae, DIY microscopy, DNA testing, and collaborations on projects like synthetic biology and environmental monitoring.
1. The document discusses the speaker's experiences with biohacking and DIY biology, including early work bridging material science and biology, developing workshops for various groups, and collaborating on projects like algae farming and open source biological art.
2. It describes the concept of biohacking as community-driven biology involving topics like genetic engineering, DNA testing, and environmental monitoring. The goal is democratization of tools and knowledge.
3. The speaker advocates for an approach of DIY and DIWO (Do It Yourself/With Others) to create useful or beautiful things through collaborative development and sharing of knowledge via online resources like the Hackteria wiki.
This document discusses DIY (do-it-yourself) microscopy and building microscopes. It mentions the author's experiences constructing microscopes using inexpensive materials and providing workshops to teach these skills globally. Specific projects are noted, such as building stable microscope stages and observing dancing tardigrades. The goal is to demystify science and make microscopy accessible through open source plans and a shared knowledge base.
Personal Introduction, from NanoBioInterfaces to stuffing rats. From Hackteria and global Biohackers to Smart Coconuts and low-cost electronics educational tools
dusjagr & nano talk on open tools for agriculture research and learningMarc Dusseiller Dusjagr
Open Tools for Research, Learning, Sharing in Agriculture and
Society as a whole.
Remote lecture by Marc Dusseiller and Fernando "nano" Castro on open science hardware for agriculture and edication, held for students of microbiology at UGM, Yogyakarta (remote - online )
UGM 2022: Open Source Biological Art and DIY / DIWO Scientific InstrumentsMarc Dusseiller Dusjagr
Overview of transdisciplinary approach bridging art and science in the global hackteria network.
Concrete examples of building low-cost scientific laboratory equiment.
Various DIY electronics for data logging.
what is art?
This document provides an overview of Dr. Marc R. Dusseiller's background and work investigating transdisciplinary approaches at the intersection of nanotechnology and human genome editing. It discusses his involvement with DIY biology and open source laboratory infrastructure projects since the 1980s aimed at making science more accessible. Specific projects mentioned include Hackteria workshops around the world to share skills for citizen science, as well as investigations into using nanoparticles for human genome editing and setting up a nano lab in a former bar in Slovenia.
Throughout the course, we will lift the fogs of these superficial discussions and through hands-on activities get closer to grasp the world of the small. We will build our own simple DIY (do-it-yourself) optical and electronic instruments to learn more about nanoparticles and nanosensors, how to “see” them, how to “hear” them.
During the 10 years of Hackteria.org we have established a global network and online knowledge base enabling practioners from diverse backgrounds to experiment with life science methodologies, from laboratory biology and genetics to environmental monitoring or fermentation. We have explored methods of collaboration, established in the early interenet culture, such as hackathons or docusprints as offline physical face-to-face production sessions, aswell as online tools for co-writing text-based instructions and lab notes. In this talk, I will give an overview of how our DIWO (Do-It-With-Others) method has lead to highly innovative projects, low-cost laboratory infrastructures, playful and critical prototypes, new workshop methods embracing a radically transdisciplinary approach to bridge the arts and the sciences.
Seoul 2019 - “Hackteria | Open Source Biological Art” - Transdisciplinary Ap...Marc Dusseiller Dusjagr
During the 10 years of Hackteria.org we have established a global network and online knowledge base enabling practioners from diverse backgrounds to experiment with life science methodologies, from laboratory biology and genetics to environmental monitoring or fermentation. We have explored methods of collaboration, established in the early interenet culture, such as hackathons or docusprints as offline physical face-to-face production sessions, aswell as online tools for co-writing text-based instructions and lab notes. In this talk, I will give an overview of how our DIWO (Do-It-With-Others) method has lead to highly innovative projects, low-cost laboratory infrastructures, playful and critical prototypes, new workshop methods embracing a radically transdisciplinary approach to bridge the arts and the sciences.
Presentation of the founding and background of Hackteria | Open Source Biological Art
Examples of modes of temporary collaborations during retreats, labs, camps and more.
WHAT IS/NOT WORKING
*/ Sesi konversasi mengenal kerja bersama
Organized along with Kunci and lifepatch, the event marks Hackteria’s tenth year as a global network that involves the collaboration of scientists, makers, hackers and artists in exploring new possibilities in bioart, open source hardware/software and experiments with biology, art/science based in various locations.
Instead of denoting what has been succeeded and how to pursue it, the conversation will explore possibilities of what could enable working together based on Kunci’s, Hackteria’s and lifepatch’s experience. The question ‘what is/not working’ may imply a ceased productivity, and at the same time, may provide opportunities to discover distinct values based on “non-productive” issues.
An overview of the 10 years history of Hackteria, establishing a global network of transdisciplinary collaborations and bioart workshops. Various examples of DIY science instruments, from rough educational prototypes to advanced open hardware for science.
This document provides an overview of the speaker's experiences with DIY biology and biohacking. It discusses the development of generic laboratory infrastructure to support citizen science, knowledge sharing and open source hardware. It describes various workshops and labs focused on transdisciplinary approaches at the intersection of biology, art, and design. Examples include DIY microscopy, synthetic biology competitions, environmental monitoring, and body hacking. The document emphasizes that biohacking has always existed as people find novel ways to apply science in their daily lives.
Creative PCB Design for Manufacturing using svg2shenzhen dusjagr taipeiMarc Dusseiller Dusjagr
I have explored many ways to make creative PCB designs for simple workshops in electronics and soldering, as part of the Swiss Mechatronic Art Society and in collaboration with other geeks worldwide. Pencil drawing, inkscape, homemade etching and in the end... creating Gerber files for Manufacturing using the amazing tool by Budi Prakosa "svg2shenzhen" to export from Inkscape to KiCad and produce nice gerber files. These slides are an overview of many examples over the years that lead to the most recent tool and PCBs.
This document provides an overview of DIY biology and biotechnology ("biohacking") through examples of workshops, labs, and projects. It discusses the global DIYbio community and efforts to provide open infrastructure like websites, instructions, and DIY lab instruments. Examples include homemade microscopy, PCR thermocyclers from hacked hairdryers, DNA testing, tissue engineering, fermentation, and mobile labs in Indonesia. The goal is democratizing access to tools and knowledge through a transdisciplinary approach involving artists, designers, researchers and citizens.
Overview of dusjagr's background from nanobiotechnology to making cheese and global workshopology. The role of temporary labs for collaborative prototyping, examples from Taiwan, Indonesia and Switzerland. Hackerspaces crossing digital- and biotechnologies.
This document discusses biohacking and open source biology. It begins by explaining that biohacking is about life and democratizing access to tools and knowledge. This includes topics like genetic engineering, DNA testing, and building one's own laboratory. The document then highlights several examples of open source biological art and DIY biology projects. It emphasizes building communities around collaborative development and workshopping. The goal is to make science more open and accessible to all.
- Dr. Marc Dusseiller discusses his experiences with biohacking and DIY (do-it-yourself) biology over the past 10+ years, including workshops held around the world.
- He helped establish hackerspaces and labs that merge digital and biological technologies to democratize science tools and knowledge.
- Examples include microfarming algae, DIY microscopy, DNA testing, and collaborations on projects like synthetic biology and environmental monitoring.
1. The document discusses the speaker's experiences with biohacking and DIY biology, including early work bridging material science and biology, developing workshops for various groups, and collaborating on projects like algae farming and open source biological art.
2. It describes the concept of biohacking as community-driven biology involving topics like genetic engineering, DNA testing, and environmental monitoring. The goal is democratization of tools and knowledge.
3. The speaker advocates for an approach of DIY and DIWO (Do It Yourself/With Others) to create useful or beautiful things through collaborative development and sharing of knowledge via online resources like the Hackteria wiki.
This document discusses DIY (do-it-yourself) microscopy and building microscopes. It mentions the author's experiences constructing microscopes using inexpensive materials and providing workshops to teach these skills globally. Specific projects are noted, such as building stable microscope stages and observing dancing tardigrades. The goal is to demystify science and make microscopy accessible through open source plans and a shared knowledge base.
Personal Introduction, from NanoBioInterfaces to stuffing rats. From Hackteria and global Biohackers to Smart Coconuts and low-cost electronics educational tools
HSLU Open - Dusseiller April2017 - Beyond Smart Coconuts
Mis lecture interfaces_introduction
1. Slide 1Mikrosysteme – Interfaces
GrundlagenGrundlagen
Mikro- und NanosystemeMikro- und Nanosysteme
Mikro- und Nanosysteme in der Umwelt, Biologie und MedizinMikro- und Nanosysteme in der Umwelt, Biologie und Medizin
Interfaces, EinführungInterfaces, Einführung
Dr. Marc R. DusseillerDr. Marc R. Dusseiller
2. Slide 2Mikrosysteme – Interfaces
InterfacesInterfaces
Oberflächenchemie und -ladungOberflächenchemie und -ladung
AdsorbtionsmechanismenAdsorbtionsmechanismen
SAMsSAMs
PolyelektrolytePolyelektrolyte
ProteineProteine
OberflächenanalytikOberflächenanalytik
3. Slide 3Mikrosysteme – Interfaces
Verfahren in der MikrosystemtechnikVerfahren in der Mikrosystemtechnik
Vielfalt an Anwendungen erfordert vielzahl an VerfahrenVielfalt an Anwendungen erfordert vielzahl an Verfahren
Surface functionalizationSurface functionalization
9. Slide 9Mikrosysteme – Interfaces
OberflächenladungOberflächenladung
Metalloberflächen haben immer eine Oxidschicht, die im wässrigenMetalloberflächen haben immer eine Oxidschicht, die im wässrigen
Millieu Ladungen aufnimmtMillieu Ladungen aufnimmt
10. Slide 10Mikrosysteme – Interfaces
Elektrische DoppelschichtElektrische Doppelschicht
Verteilung der Ladungsträger (Ionen) in der GrenzschichtVerteilung der Ladungsträger (Ionen) in der Grenzschicht
11. Slide 11Mikrosysteme – Interfaces
Elektrische DoppelschichtElektrische Doppelschicht
Änderung der Ladungsverteilung in der GrenzschichtÄnderung der Ladungsverteilung in der Grenzschicht
23. Slide 23Mikrosysteme – Interfaces
Polyelektrolyte - AnwendungPolyelektrolyte - Anwendung
Anwendung von PML für Triggered Drug-Release SystemAnwendung von PML für Triggered Drug-Release System
24. Slide 24Mikrosysteme – Interfaces
ProteinadsorbtionProteinadsorbtion
Was sind ProteineWas sind Proteine
ISF is a flat spherical molecule with dimensions of 45 x 40 x 25 Å
25. Slide 25Mikrosysteme – Interfaces
ProteinadsorbtionProteinadsorbtion
Wieso adsorbieren Proteine?Wieso adsorbieren Proteine?
• Hydrophobe WechselwirkungHydrophobe Wechselwirkung
Weniger Interaktion mit WasserWeniger Interaktion mit Wasser
• Elektrostatische WechselwirkungElektrostatische Wechselwirkung
Proteine und Oberflächen sind geladenProteine und Oberflächen sind geladen
• ππ-- ππ BindungBindung
Kohlenstoff RingeKohlenstoff Ringe
• Ionenbrücken-BindungIonenbrücken-Bindung
Mittels divalenter Metall-Ionen (CaMittels divalenter Metall-Ionen (Ca++++
, Zn, Zn++++
...)...)
StärkederInteraktion
27. Slide 27Mikrosysteme – Interfaces
ProteinadsorbtionProteinadsorbtion
Vroman EffektVroman Effekt
Zuerst die kleinenZuerst die kleinen
Dann die grossenDann die grossen
29. Slide 29Mikrosysteme – Interfaces
Brush Polymere – PLL-g-PEGBrush Polymere – PLL-g-PEG
OO22 Plasma Behandlung von PS or PDMSPlasma Behandlung von PS or PDMS
• PS: Oberflächenoxidation - negative charges (COOPS: Oberflächenoxidation - negative charges (COO--
))
• PDMS: Glassartige Oberfläche (SiOPDMS: Glassartige Oberfläche (SiO22))
PLL-PLL-gg-PEG-PEG
Poly(L-Lysine)Poly(L-Lysine) graftedgrafted Poly(Ethylene Glycol)Poly(Ethylene Glycol)
• self assembly auf negativ geladenen Oberflächenself assembly auf negativ geladenen Oberflächen
Metalloxide, behandelte PolymereMetalloxide, behandelte Polymere
• resistent gegen Proteinadsorbtionresistent gegen Proteinadsorbtion
• funktionelle Gruppenfunktionelle Gruppen
Peptidsequenzen (cont. RGD - integrin binding)Peptidsequenzen (cont. RGD - integrin binding)
Spezifische Bindungsstellen (zb.PLL-Spezifische Bindungsstellen (zb.PLL-gg-PEG/biotin o.-PEG/biotin o.
NTA)NTA)
30. Slide 30Mikrosysteme – Interfaces
NanoarchitekturNanoarchitektur
Aufbau einer intelligenten Nanoarchitektur in einem Prozess mitAufbau einer intelligenten Nanoarchitektur in einem Prozess mit
mehreren Schritten und hoher Selektivität (DNA Hybridisierung)mehreren Schritten und hoher Selektivität (DNA Hybridisierung)
Passivierung (reduktion der unspezifischen Proteinadsorbtion)Passivierung (reduktion der unspezifischen Proteinadsorbtion)
zwischen den Binding Sites (PEG-biotin) durch PEGzwischen den Binding Sites (PEG-biotin) durch PEG
Vesicles aus Lipiden können heikle Membranproteine in derVesicles aus Lipiden können heikle Membranproteine in der
Bilayerschicht enthalten oder Biomoleküle in ihrem VolumenBilayerschicht enthalten oder Biomoleküle in ihrem Volumen
aufbewahrenaufbewahren
31. Slide 31Mikrosysteme – Interfaces
NanoarchitekturNanoarchitektur
Andere Funktionalisierungsmethoden auf PEG BasisAndere Funktionalisierungsmethoden auf PEG Basis
32. Slide 32Mikrosysteme – Interfaces
SMAPSMAP
Selective Molecular Assembly PatterningSelective Molecular Assembly Patterning
Selektive Chemie auf OxidpatternsSelektive Chemie auf Oxidpatterns
35. Slide 35Mikrosysteme – Interfaces
OberflächenanalytikOberflächenanalytik
AnwendungenAnwendungen
• GrundlagenforschungGrundlagenforschung
• Qualitätskontrolle während der Produktion und EntwicklungQualitätskontrolle während der Produktion und Entwicklung
Beschichtungen, Verunreinigungen etc.Beschichtungen, Verunreinigungen etc.
• Untersuchen von Implantaten / Grenzefläche Material - KörperUntersuchen von Implantaten / Grenzefläche Material - Körper
36. Slide 36Mikrosysteme – Interfaces
FluoreszenzFluoreszenz
• Durch Absorbtion wird ein Elektron in einenDurch Absorbtion wird ein Elektron in einen
energetisch höheren Zustand versetztenergetisch höheren Zustand versetzt
• Nach geringer Relaxation springt es zurückNach geringer Relaxation springt es zurück
und emitiert ein Photon mit geringererund emitiert ein Photon mit geringerer
Energie, dh. grösserer WellenlängeEnergie, dh. grösserer Wellenlänge
• Zeitskala ist sehr kurz bei FluoreszenzZeitskala ist sehr kurz bei Fluoreszenz
• Kann bei Atomen, Molekülen oderKann bei Atomen, Molekülen oder
Nanopartikel vorkommenNanopartikel vorkommen
• Kann gebleicht werdenKann gebleicht werden
37. Slide 37Mikrosysteme – Interfaces
Fluoreszierende Marker / LabelsFluoreszierende Marker / Labels
Es gibt eine Vielzahl an fluoreszierenden MolekülenEs gibt eine Vielzahl an fluoreszierenden Molekülen
• Ganz kleine zb. FITC (fluorescein)Ganz kleine zb. FITC (fluorescein)
• Ganze Proteine zb. GFP kannGanze Proteine zb. GFP kann in vivoin vivo benutzt werdenbenutzt werden
(Green Fluorescent Protein)(Green Fluorescent Protein)
• Nanopartikel (Quantumdots)Nanopartikel (Quantumdots)
Eigene Absorbtion/Emission CharakteristikEigene Absorbtion/Emission Charakteristik
FITCFITC GFPGFP QDOTsQDOTs
40. Slide 40Mikrosysteme – Interfaces
SPRSPR
Surface Plasmon ResonanceSurface Plasmon Resonance
• Sehr sensitivSehr sensitiv
• label-freelabel-free
• imaging möglich (10imaging möglich (10 μμm Auflösung)m Auflösung)
• Nur auf Gold, DünnschichtNur auf Gold, Dünnschicht
• Biacore Life SciencesBiacore Life Sciences
http://www.biacore.com/lifesciences/technology/introduction/data_interaction/index.htmlhttp://www.biacore.com/lifesciences/technology/introduction/data_interaction/index.html
41. Slide 41Mikrosysteme – Interfaces
EllipsometrieEllipsometrie
Analyse von der Reflektion von polarisiertem LichtAnalyse von der Reflektion von polarisiertem Licht
• Optische Eigenschaften von Material und BeschichtungenOptische Eigenschaften von Material und Beschichtungen
• Dicke von dünnen FilmenDicke von dünnen Filmen
• Imaging möglich (2Imaging möglich (2 μμm Auflösung)m Auflösung)
• Nur reflektierende OberflächenNur reflektierende Oberflächen
• Planare OberflächenPlanare Oberflächen
• ModelabhängigkeitModelabhängigkeit