1. Honolulu Technique
By: Sydney Dudley

2. What is Cloning?
• Cloning refers to the development of offspring
that are genetically identical to their parent.
• There are two techniques for cloning:
– The Roslin Technique
– The Honolulu Technique

3. Cloning 101
• http://www.youtube.com/watch?v=q0B9Bn1
WW_4&safety_mode=true&persist_safety_m
ode=1&safe=active

4. Background
• Founded in 1998 byTeruhiko Wakayama and
Ryuzo Yanagimachi of the University of Hawaii
• This technique has successfully cloned five
generations of mice.

5. Step 1
• Nucleus of a cell is extracted from the
organism that is being cloned
– Cumulus cells are the most commonly used
• Cumulus cells surround an egg cell.
Egg Cumulus
cell cells

6. Step 2
• Nucleus is removed from an unfertilized egg
cell.

7. Step 3
• The nucleus from the first cell is injected into
the enucleated egg cell.

8. Step 4
• The new egg cell is placed into a chemical
bath to jumpstart cell growth.
– Chemical bath is less harmful than electric shock
used in the Roslin Technique.

9. Step 5
• The cells develop into embryos and are placed
in a surrogate mother and carried to term.

10. Success Rate
• Honolulu technique- 3 clones out of every 100
attempts.
• Roslin technique- 1 clone out of every 277
attempts.

11. Uses
• The Honolulu Technique can be used for:
– Research of diseases such as AIDS, cancer, or
diabetes
– Genetically alter animals for the production of
human transplant organs
– NOT intended for the cloning of humans

12. Arguments Against Cloning
• Scientific:
– Low success rate
– Clones don’t live as long
– Decreases genetic diversity
• Practical
– Easier to produce natural offspring
• Religious
– Human life is precious in God’s eyes
– People cloned for “spare parts”
– Men should not play the role of God