Poster presented at SAMS, May 2015. AIM - To create a germplasm bank for 3 different populations of the giant kelp in the coast of Chile and assess its viability after 5 years in suspended state.

1. Macrocystis pyrifera life in suspended state
up to 54%
viable
2010
2011 2012
0
20
40
60
South
Centre
North
WHYAIM
To create a germplasm
bank for 3 different
populations of the giant
kelp in the coast of Chile
and assess its viability
after 5 years in
suspended state
Higher production demand
for abalone feed
Aquaculture management
Genetic stock of 3 different
populations from the
North, Centre and South
WHERE
HOW
Male and female
gametophytes were
collected from North,
Centre and South in 3
consecutive years and
stocked in controled
conditions (10°C; 2-4
μmol m-2
s-1
; in Provasoli)
2010
2011
2015
2012
Sample collection
dates.
All populations:
North, Centre and
South, were
collected in each
year and stocked
individually by
sex.
Viability check
based on
morphology and
pigments
Morphology and pigment criteria to assess viability. Top
left – freshly released gametophytes (sporulation
December 2014); Top right - Male gametophyte from
Choros, 2010 (viable); Bottom left - Female
gametophyte from Choros, 2010 showing lack of
pigmentation; Bottom right - Female gametophyte from
Puchilco, 2010; no pigments
23048
gametophytes
not related with stocking duration
depends on genetic population
50 % viable in the Centre
population
6 % viable in the South population,
2012
VIABILITY SO WHAT?
After5yearsinsuspendedstate
Unique bank in the world
Efficient method to preserve
gametophytes up to 5 years
Assessment after 5 years in
suspended state, with 50% viability
Stocking individuals by sex allows
future genetic improvement
Gametophytes can also be used for:
conservation purposes
population restocking
aquaculture management
toxicological research
gametogeneses studies
….
Seeking opportunity to develop a
joint program to develop an
international bank of different
species and populations worldwide.
Sara Barrento
Carolina Camus
Isabel Sousa Pinto
Alejandro H.
Buschmann
Interdisciplinary Centre of Marine and Environmental Research, Porto, Portugal;
sarixbarrento@gmail.com
Consorcio BalBiofuels & CeBiB, Puerto Montt, Chile; Centro i~mar, Puerto Montt, Chile.
Interdisciplinary Centre of Marine and Environmental Research, CIIMAR, Porto, Portugal
Centro i~mar & CeBiB, Universidad de Los Lagos, Puerto Montt, Chile.
The first author acknowledges a pos doc fellowship of the
Portuguese Foundation for Science and Technology (FCT)
(Ref. SFRH/BPD/78211/2011, and AHB thanks the support
of Innova-Corfo and FB-0001