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Comparison between Potato cultivarsComparison between Potato cultivars
& Abiotic stress genes& Abiotic stress genes
Comparison between Potato cultivarsComparison between Potato cultivars
& Abiotic stress genes& Abiotic stress genes
Supervisor:Supervisor:
Dr. Mohamed ElseehyDr. Mohamed Elseehy
Presenter:Presenter:
Sara F. ShaheenSara F. Shaheen
Sara.fkrey@hotmail.com
• Potato production is one of the main agricultural activities
worldwide for both economic and social reasons.
• The total world potato production in 2012 reached was 364 million
ton (FAOSTAT 2014).
• Potato production in Africa has been exponentially13 million tons
in 2000 expanding reaching30 million tons in 2014 (FAOSTAT2016).
Potato production share by region Average
2000 - 2014
Source: http://faostat3.fao.org/browse/Q/QC/E
• Potato (Solanum tuberosum L.) is the most important export
vegetable crop in Egypt and the second most important
vegetable crop after tomato in economic value. (El-Tobgy, 1974).
• Egypt is one of the top 20 producers of potato worldwide and
the largest producer and exporter of potatoes in Africa.
South Africa
South Africa
South Africa
1,934,492 tones
Kenya
Kenya
1,948,326 tones
EGYPTEGYPT
3,167,7473,167,747 tonestones
Algeria
2,591,873 tones
Morocco
1,496,290 tones
Source:http://www.freshplaza.com/article/153030/Glo
bal-export-potatoes-stop-growing
• In 2014, Egypt potato production was 4,8 million ton
(FAOSTAT). Including 637,434 ton for exportation a market
value of 250 million USD
• About 20% of total area devoted for vegetable production is
cultivated with potato in Egypt. (FAOSTAT 2007).
• More than 105 thousand ha are dedicated for potato production
(FAOSTAT 2007).
Growth rate calculated based on the Least-Squares method Source: http://faostat3.fao.org/browse/Q/QC/E
Production of Potato in Egypt
2000-2014
Yield of Potato in Egypt
2000-2014 Source: http://faostat3.fao.org/browse/Q/QC/E
Source: https://www.sasa.gov.uk/seed-ware-potatoes/potato-exports
Egypt achieved positive results in improving quality seed
potato through the implementation of the rules and
regulations of:
Climate change & PotatoClimate change & Potato
• potato cultivation is facing several challenges to maintain
and improve production. like highly sensitive to the
projected climate changes
Source: http://cipotato.org/press-room/blog/el-ninos-effects-in-asia-point-to-
new-tactics-to-adapt-to-climate-change/
Source:http://www.ipcc.ch/graphics/graphics/2001wg1/large/05.02.jpg
Temperature
Quiroz et al., 2011
Effects of Temperature on PotatoEffects of Temperature on Potato
Wet and Dry Days Changes 1880:2080Wet and Dry Days Changes 1880:2080
Quiroz et al., 2011
Extreme Event Changes 1880:2080Extreme Event Changes 1880:2080
Quiroz et al., 2011
• Abiotic stress is the primary cause of crop loss worldwide.
• It was estimated that approximately 70 % of yield reduction
was due to abiotic stress ( Acquaah, 2007)
• The estimated potential yield losses are 17 % due to
drought, 20 % due to salinity, 40 % due to high temperature,
15 % due to low temperature and 8 % due to other stress
( Ashraf et al., 2008)
Plant ABIOTIC stressPlant ABIOTIC stress
StressStress ConsequencesConsequences Plant ResponsesPlant Responses
Heat stress
• High temperature lead to high
evaporation and water deficit.
• reduce tuber initiation
• reducing potato growth
• reduces root, stolon, tuber and
total DM and total tuber number
• Efficient protein repair systems and
general protein stability support survival
• temperature can lead to acclimation.
Chilling & cold
stress
• Biochemical reactions proceed at
slower rate like photosyntesis
proceeds
• Indeed, freezing lead to ice
crystal formation that can
damage cells membranes.
• Stop of growth in adaptable species may
be overcome by changes in metabolism.
Drought
• Inability to water transport to
leaves leads to photosyntesis
declines.
• Leaf rolling and other morphological
adaptations.
• Stoma closure reduces evaporative
transpiration induced by ABA.
• consequently lower internal water
potential and water attracting.
Source: Loredana et al., 2011
HeatHeat stressstress
Stress resistance mechanisms
1. Avoidance mechanisms
– Prevents coverage to stress
2. Tolerance mechanisms
– Produce the plant to resist stress
3. Acclimation
– Modify their physiology in response stress
Ahuja et al., 2010
Genes linked to more than one abiotic stresses
Ahuja et al., 2010
“TPS1 Gene”
trehalose-6-phosphate synthase gene
“TPS1 Gene”
trehalose-6-phosphate synthase gene
Aim of the work:Aim of the work:
Objectives:Objectives:
Methodology:Methodology:
1.Conducting the survey
• A survey will be developed based on export
quality specifications to collect data from Potato
Farms about potato varieties planted in these
farms and the varieties' characters.
• In addition, Samples will be collected from Potato
Farms for each Variety.
2. Comparison between varieties
• Genomic DNA will be extracted
• Then Polymerase Chain Reaction PCR will be
done using an Specific primers with genomic
DNA (gDNA) as it’s template of Abiotic stress
resistance gene.
• Use of agarose gel electrophoresis of PCR
amplification products
• Data analysis.
3. Comparison between Results from PCR
and Survey Data
• Which cultivar has Abiotic stress resistance
gene as well as export quality specifications
Abiotic stress tolerance isAbiotic stress tolerance is
complex!!complex!!
Potato & climate change

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Potato & climate change

  • 1. Comparison between Potato cultivarsComparison between Potato cultivars & Abiotic stress genes& Abiotic stress genes Comparison between Potato cultivarsComparison between Potato cultivars & Abiotic stress genes& Abiotic stress genes Supervisor:Supervisor: Dr. Mohamed ElseehyDr. Mohamed Elseehy Presenter:Presenter: Sara F. ShaheenSara F. Shaheen Sara.fkrey@hotmail.com
  • 2. • Potato production is one of the main agricultural activities worldwide for both economic and social reasons. • The total world potato production in 2012 reached was 364 million ton (FAOSTAT 2014). • Potato production in Africa has been exponentially13 million tons in 2000 expanding reaching30 million tons in 2014 (FAOSTAT2016). Potato production share by region Average 2000 - 2014 Source: http://faostat3.fao.org/browse/Q/QC/E
  • 3. • Potato (Solanum tuberosum L.) is the most important export vegetable crop in Egypt and the second most important vegetable crop after tomato in economic value. (El-Tobgy, 1974). • Egypt is one of the top 20 producers of potato worldwide and the largest producer and exporter of potatoes in Africa.
  • 4. South Africa South Africa South Africa 1,934,492 tones Kenya Kenya 1,948,326 tones EGYPTEGYPT 3,167,7473,167,747 tonestones Algeria 2,591,873 tones Morocco 1,496,290 tones
  • 6. • In 2014, Egypt potato production was 4,8 million ton (FAOSTAT). Including 637,434 ton for exportation a market value of 250 million USD • About 20% of total area devoted for vegetable production is cultivated with potato in Egypt. (FAOSTAT 2007). • More than 105 thousand ha are dedicated for potato production (FAOSTAT 2007).
  • 7. Growth rate calculated based on the Least-Squares method Source: http://faostat3.fao.org/browse/Q/QC/E
  • 8. Production of Potato in Egypt 2000-2014 Yield of Potato in Egypt 2000-2014 Source: http://faostat3.fao.org/browse/Q/QC/E
  • 9. Source: https://www.sasa.gov.uk/seed-ware-potatoes/potato-exports Egypt achieved positive results in improving quality seed potato through the implementation of the rules and regulations of:
  • 10. Climate change & PotatoClimate change & Potato • potato cultivation is facing several challenges to maintain and improve production. like highly sensitive to the projected climate changes Source: http://cipotato.org/press-room/blog/el-ninos-effects-in-asia-point-to- new-tactics-to-adapt-to-climate-change/
  • 12. Temperature Quiroz et al., 2011 Effects of Temperature on PotatoEffects of Temperature on Potato
  • 13. Wet and Dry Days Changes 1880:2080Wet and Dry Days Changes 1880:2080 Quiroz et al., 2011
  • 14. Extreme Event Changes 1880:2080Extreme Event Changes 1880:2080 Quiroz et al., 2011
  • 15. • Abiotic stress is the primary cause of crop loss worldwide. • It was estimated that approximately 70 % of yield reduction was due to abiotic stress ( Acquaah, 2007) • The estimated potential yield losses are 17 % due to drought, 20 % due to salinity, 40 % due to high temperature, 15 % due to low temperature and 8 % due to other stress ( Ashraf et al., 2008) Plant ABIOTIC stressPlant ABIOTIC stress
  • 16.
  • 17. StressStress ConsequencesConsequences Plant ResponsesPlant Responses Heat stress • High temperature lead to high evaporation and water deficit. • reduce tuber initiation • reducing potato growth • reduces root, stolon, tuber and total DM and total tuber number • Efficient protein repair systems and general protein stability support survival • temperature can lead to acclimation. Chilling & cold stress • Biochemical reactions proceed at slower rate like photosyntesis proceeds • Indeed, freezing lead to ice crystal formation that can damage cells membranes. • Stop of growth in adaptable species may be overcome by changes in metabolism. Drought • Inability to water transport to leaves leads to photosyntesis declines. • Leaf rolling and other morphological adaptations. • Stoma closure reduces evaporative transpiration induced by ABA. • consequently lower internal water potential and water attracting. Source: Loredana et al., 2011
  • 19. Stress resistance mechanisms 1. Avoidance mechanisms – Prevents coverage to stress 2. Tolerance mechanisms – Produce the plant to resist stress 3. Acclimation – Modify their physiology in response stress
  • 21. Genes linked to more than one abiotic stresses Ahuja et al., 2010
  • 22. “TPS1 Gene” trehalose-6-phosphate synthase gene “TPS1 Gene” trehalose-6-phosphate synthase gene
  • 23.
  • 24. Aim of the work:Aim of the work:
  • 26. Methodology:Methodology: 1.Conducting the survey • A survey will be developed based on export quality specifications to collect data from Potato Farms about potato varieties planted in these farms and the varieties' characters. • In addition, Samples will be collected from Potato Farms for each Variety.
  • 27. 2. Comparison between varieties • Genomic DNA will be extracted • Then Polymerase Chain Reaction PCR will be done using an Specific primers with genomic DNA (gDNA) as it’s template of Abiotic stress resistance gene. • Use of agarose gel electrophoresis of PCR amplification products • Data analysis.
  • 28. 3. Comparison between Results from PCR and Survey Data • Which cultivar has Abiotic stress resistance gene as well as export quality specifications
  • 29. Abiotic stress tolerance isAbiotic stress tolerance is complex!!complex!!