1. PROTEINSIMPLE REINVENTS THE WESTERN BLOT and FASTER, SCALABLE METHOD FOR PRODUCING AAV-BASED GENE TRANSFER VECTORS Maria Sarah Restrepo Medicine student

2. FOLDING Maria Sarah Restrepo

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4. INTRODUCTION A REVOLUTIONARY SYSTEM FOR PROTEIN ANALYSIS, THAT HELPS IN A BETTER WAY, TO UNDERSTAND THE NATURE OF PROTEINS IN DISEASES. NEW METHOD FOR THE STUDIED OF VIRAL VECTORS THAT HAS BEEN DEVELOPED, TO SIMPLIFY EVERY STEP OF THE PROCEDURE.

5. PROTEINSIMPLE REINVENTS THE WESTERN BLOT Marketwatch. August 30-2011

6. PROTEINSIMPLE REINVENTS THE WESTERN BLOT ProteinSimple today introduced a revolutionary new system for protein analysis, Simon and the Simple Western. The Simple Western is a complete reinvention of the Western Blot, and is truly a historic breakthrough for protein research. For the first time in 30 years, researchers have access to a completely automated, walk-away solution for Western analysis.

7. PROTEINSIMPLE REINVENTS THE WESTERN BLOT The Western Blot was invented in 1979 and to this day remains the most frequently used technique for protein characterization. Traditional Westerns require many manual steps using gels, transfer tanks, blots and film, and a single experiment can take up to 24 hours to complete.

8. PROTEINSIMPLE REINVENTS THE WESTERN BLOT The Simple Western is a Western with none of the hassle. No messy gels, no transfer tanks, no blots, no imaging and no manual analysis. Simply load your samples into Simon, push a button, walk away and come back a few hours later to fully analyzed data.

9. PROTEINSIMPLE REINVENTS THE WESTERN BLOT OBSERVATIONS This revolutionary discovery only proves the magnificent importance of technology in Medicine Procedures. With the Simple Western, researchers not only economice time and Money, but gain a faster and developed way to investigate the Proteins and their influence in some di- seases.

10. FASTER, SCALABLE METHOD FOR PRODUCING AAV-BASED GENE TRANSFER VECTORS Science Daily. January 05-2011

11. FASTER, SCALABLE METHOD FOR PRODUCING AAV-BASED GENE TRANSFER VECTORS A new, simplified method for producing large amounts of viral vector cassettes capable of shuttling genes into host cells will help advance the promising field of gene therapy as applications move into large animal studies and human clinical trials.

12. FASTER, SCALABLE METHOD FOR PRODUCING AAV-BASED GENE TRANSFER VECTORS This new method for creating stable AAV2 producer cell lines overcomes the major drawbacks of the previous method: it requires only a one-step cloning process (instead of multiple cloning steps).

13. FASTER, SCALABLE METHOD FOR PRODUCING AAV-BASED GENE TRANSFER VECTORS In addition to being faster and easier, their simplified method offers other important advantages, including the potential to produce high yields of multiple AAV serotypes upon co-infection with a helper adenovirus.

14. FASTER, SCALABLE METHOD FOR PRODUCING AAV-BASED GENE TRANSFER VECTORS AAV gene therapy is showing promise in the clinic. Important challenges exist for it to become a commercial product such as scalable manufacturing processes.

15. OBSERVATIONS A versatil method like this new one, can be an ex- traodinary and useful tool for the scientific commu- nity, reducing the steps of de older method and be- coming a way to develop the AAV genes therapies, that today have the potential to be a commercial product. FASTER, SCALABLE METHOD FOR PRODUCING AAV-BASED GENE TRANSFER VECTORS

17. With the simply Western, is easier to understand the behavior of proteins and everything that tours around them. PROTEINSIMPLE REINVENTS THE WESTERN BLOT

18. With the new Method for the stu- dy of AAV gene vector, the scienti- fic community is able to put on the medical market a very important treatment as the AAV gene thera- py with more certain results. FASTER, SCALABLE METHOD FOR PRODUCING AAV-BASED GENE TRANSFER VECTORS

19. BIBLIOGRAPHY ProteinSimple Reinvents the Western Blot. Marketwatch [online]. [ref. Agost 30, 2011]. Available online http://www.marketwatch.com/story/protei nsimple-reinvents-the-western-blot-2011- 08-30  Faster, Scalable Method for Producing AAV-Based Gene Transfer Vectors. Science Daily [online]. [ref. January 5, 2011]. Avai- lable online <http://www.sciencedaily.com/releases/2

20. THANKS