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Ch 4: Cellular Metabolism, Part 1 ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Energy in Biol. Systems: ,[object Object],[object Object],[object Object],[object Object],Fig 4-2 2 types of energy: ,[object Object],[object Object],[object Object],[object Object]
Energy (E) Transfer Overview Figure 4-1
Potential Energy Kinetic Energy WORK heat  (~ 70% of energy used in physical exercise)
Bioenergetics =   study of energy flow  through biol. systems ,[object Object],[object Object],Substrates or  reactants Products Speed of reaction = Reaction rate Initial force = Activation Energy
Potential Energy Stored in Chemical Bonds of Substrate can be . . . ,[object Object],[object Object],[object Object]
Chemical Reactions  p 93 ,[object Object],[object Object],[object Object],[object Object],[object Object]
Activation Energy Fig 4-3
Endo- and Exergonic Reactions Which is which??  ATP + H 2 O  ADP + P i  + H +  + Energy
Enzyme (= Biol. Catalyst) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Some important characteristics of an enzyme: Fig 4-6
 
Enzymes lower activation energy: All chemical reactions in body must be conducted at body temp.!! How do enzymes lower activation energy ?
[object Object]
Some more characteristics of enzymes: ,[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],Active Site: Old: Lock-and-key model / New: Induced-fit model
Enzyme-substrate interaction:  The old and the new model Lock and Key: Induced fit:
Enzyme Specificity ,[object Object],[object Object]
Naming of Enzymes ,[object Object],[object Object],[object Object],[object Object],mostly suffix  -ase first part gives info on function examples
Isoenzymes =  different models of same  enzyme (differ in 1 or few aa) ,[object Object],[object Object],[object Object],Catalyze same reaction but under different  conditions and in different tissues/organs
Enzyme Activity  depends on ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
1) Proteolytic Activation  ,[object Object],[object Object],[object Object]
2) Cofactors  &  Coenzymes structure: Inorganic molecules  (?) function: conformational change of  active site structure: Organic molecules (vitamin  derivatives, FADH 2  ....) function: act as receptors & carriers  for atoms or functional  groups that are removed  from substrate
Cofactors bind to active site
3) Breakage of intramolecular bonds lead to ?
[object Object],[object Object],Siamese Cats tyrosinase
3)
4) Molecules interacting with enzyme ,[object Object],block active site E.g.: Penicillin binds covalently (= irreversibly to important bacterial enzyme active site) Fig 4-13
4)   Molecules interacting with enzyme, cont’d ,[object Object],= end product inhibition Special case:
Allosteric Modulation
Reaction Rate Depends on Enzyme & Substrate Concentration
Reversible Reactions follow the Law of Mass Action
Three Major Types of Enzymatic Reactions: ,[object Object],[object Object],[object Object],[object Object],[object Object]
 
?
Metabolism Catabolism Anabolism next time
 

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Cell Metabolism Part 1

Hinweis der Redaktion

  1. Troponin I and T are structural components of cardiac muscle. They are released into the bloodstream with myocardial injury. They are highly specific for myocardial injury--more so than CK-MB--and help to exclude elevations of CK with skeletal muscle trauma. Troponins will begin to increase following MI within 3 to 12 hours, about the same time frame as CK-MB. However, the rate of rise for early infarction may not be as dramatic as for CK-MB. Troponins will remain elevated longer than CK--up to 5 to 9 days for troponin I and up to 2 weeks for troponin T. This makes troponins a superior marker for diagnosing myocardial infarction in the recent past--better than lactate dehydrogenase (LDH). However, this continued elevation has the disadvantage of making it more difficult to diagnose reinfarction or
  2. (LDH) Lactate dehydrogenase  - Total LDH will begin to rise 2 to 5 days after an MI; the elevation can last 10 days. 140-280 U/L Normal Adult Range: 0 - 250 U/L Optimal Adult Reading: 125
  3. This can be tested by looking at Siamese cats that have been left outdoors over winter (all black), or raised in very hot climates (all white). Of course you can also put boots on the cats and get cats with white stockings, or tape down their ears and get white ears.