Pterocarpus Marsupiam drug are mention it different type extraction, TLC, Chemical constituent

1. Presented By
Puja R. Basule
Department of Pharmaceutical Chemistry
M-pharm I - year

2. Pterocarpus marsupiam
• Synonyms:
Indian kino tree or Malabar kino,
Bijsal, vegisa Bijasar,
Hindi: vijayasar, beeja patta, bila
Marathi: asaaha, asan, asana, bibla
Tamil: acamai, acanapann
Telugu: aine, asana, beejasaramu
Kannada: baengamara, bange, bendagamara
Urdu: bijasar, dam al akhwain
• Biological source:
It consist of the wood and bark of Pterocarpus marsupiam
belonging to Family-Fabaceae (Leguminosae)

3. • Geographical indication :
It is indigenous to Nepal, Srilanka and India (inregions
of the Western Ghats in the Karnataka-Kerala region
and forest of central India)
Dose :
100 and 200 mg/kg

4. EXTRACTION OF DRUG
Extraction of main constituent is done by various methods
1. Infusion : Heartwood are filled with water in beaker and allowed to stand
overnight to give beeja wood water
2. Decoction : Dried heartwood boiled with water and filtered
3. Maceration : Dried heartwood extracted with ethanol for 1 week
4. Percolation : Coarse powder of heartwood is extracted with 95% alcohol in
strenght of 1:6
5. Hot water extraction : Powdered heartwood extracted with hot water

5. TLC Pterocarpus Marsupium
Pterocarpus
marsupium
Mobile phase Decetion Rf value
Alkaloid Toluene: EA:
Dietheylamine
(7:2:1)
Dragendroff 0.40
Steriods Toluene: EA
(9:3:0.7
UV 254nm 0.48
Tannins n-butanol:acetic
acid:water
(14:1:5)
Alc Fecl3 0.88,0.67,0.58

6. Chemical Constituents:
2-(3,4,-dihydroxyphenyl ) chromane- 3,5,7
triol

8. Uses
•Use in diarrhoea.
•Bleeding and gout problem
•Skin problem
•Intestinal parasites
•Dental problem
•Anti microbial properties
•Hyperlipidemia
•Arthritis

9. Mechanism of action
 Regeneration Beta-cell has been proposed in diabetics
rats treated with epicatechin
 It was found to increases cAMP content of islets,
increaes insulin release
 Conversion of proinsulin to insulin
 Reduces blood glucose level in type 2 DM
 The aqueous extract decreases the elevated TNF-alpha
( effect on glucose metabolism) level in Type-2 DM due
to chronic systemic inflammation

10. Some important constituent
Epicatechin
Structure:
(2R,3R)-2-(3,4-Dihydroxyphenyl)chroman-3,5,7-triol
It is isolated from ethanolic extract

11. Analogs of Epicatechin: Reduces plasma level of glucose
( increaes activity 45-60% more activity than epicatechin)

12. Liquiritigenin
Structure:
(S)-7-Hydroxy-2-(4-hydroxyphenyl)chroman-4-one;
4',7-Dihydroxyflavanone

13. Structure activity relationship:
The presence 4’- OH group in the B-Ring and double
bond between C2 and C3 is important factors for
binding and enzyme recognition

14. Isoliquiritigenin
Structure:
(E)-1-(2,4-dihydroxyphenyl)-3-(4-hydroxyphenyl)prop-2-en-1-one

15. Synthesis:
Isomerization