1. The CGC
The Vaccine and Gene Therapy Institute of Florida is an independent non-profit research
501(c)3 research institute devoted to the study of the human immune system. VGTI Florida is
about equating biomedical research in to health. Their focus is on the advancement of new
vaccines and immunotherapies to increase the invulnerable response in combating cancer
cells and transmittable illness.
The Collaborative Genomics Center at the Vaccine & Gene Therapy Institute of Florida
(VGTI Florida) supplies consultative study services that consist of entire genome microarray
analysis utilizing the Illumina ® iScan system, sequencing-based assays using the Illumina ®
Hi-Seq 2500 Sequencing System for sequencing genomic DNA, mRNA, microRNA, or ChiP,
and gene validation using the high-throughput Applied Biosystem's OpenArray ® or
Fluidigm's BioMark system. The CGC consists of an 8-member genomics team and an 8member bioinformatics team.
Their targets are these:.
To supply an SOP-driven sample acquisition, genomic study, bioinformatic reporting, and
information management framework to assist specialists with their genomics requirements
and reporting.
To assist analysts throughout the experimental design, sample collection, genomic handling
and data study processes.
To offer sophisticated bioinformatic devices in the functional study of transcriptional
signatures.
To develop information combination methods that will certainly allow teaming up teams to
benchmark their vaccine/pathogenesis/therapeutic experiments against already existing
signatures of protection, likewise with links to publically readily available datasets.
Microarray Platform:.
Illumina ® Whole Genome Expression Bead Chips.
SOP-driven full assay consists of sample banking, total RNA isolation, 1-cycle or 2-cycle
(small sample) amplification, classifying, hybridization, checking, QC, and conventional
bioinformatics report.
Ability to profile reduced lot of cells, tissues, or small quantities of blood.
Semi-automated conventional bioinformatics report consists of raw information, QC,

2. annotation, preprocessing, contrasts, unsupervised analysis, and differential gene study.
Microarray Assay Codes:.
GEN01 Microarray Illumina ® WG BeadChip, complete 1-cycle assay: includes sample
banking, overall RNA isolation, 1-cycle boosting, labeling, hybridization, checking, QC and
common bioinformatic report.
GEN02 Microarray Illumina ® WG BeadChip, complete 1-cycle assay: features sample
banking, overall RNA isolation, 1-cycle amplification, identifying, hybridization, checking, QC
and typical bioinformatic report, plus senior task and biological layout assessment.
GEN10 Bioinformatics Advanced Reporting: A typical bioinformatics report features raw data,
QC, annotation, preprocessing, contrasts, unsupervised analysis, and differential gene study.
Advanced reports feature gene set enrichment, paths, functional genomics, modeling and
publication quality figure generation.
Next Gen Sequencing System:.
Illumina ® Hi-Seq 2000 Sequencing System Platform.
DNA-seq, mRNA-seq (multiplexed), miRNA-seq, non-coding RNA seq
. Novel procedures permit small input sample product (50ng allows for )90 % coverage of
sequence complexity roughly comparable to 5,000 cells).
Digital gene phrase.
Novel records and isoform discovery.
Sequencing Assay Design:.
Because of the highly versatile Illumina ® Hi-Seq 2500 Sequencing System platform and the
ever-expanding choices for Next Generation Sequencing, a direct appointment with the CGC
and the Investigator is required prior to final study layout and prices quotes can be identified.
Nanofluidics: Fluidigm Biomark System:.
The Biomark HD system includes a microfluidics based system that allows for the use of
nanoliter sized responses. This system allows for a large array of sample sources and
applications consisting of electronic PCR, actual time PCR, targeted sequencing, SNP
evaluation and can assist solitary cell applications. The 96x96 format permits adaptability in
assay design and application, including our custom immunology panels.
Bioinformatics Core:.

3. The CGC bioinformatics group has actually produced standardized and semi-automated
bioinformatic technologies made to evaluate and recap genomic data from a number of
systems and at the capacities required for our workload of (5000+ transcriptomic
assays/year).
Standard bioinformatic records can be created on small (12 arrays/1 flow cell) to big jobs
(hundred's of samples) in a matter of hours.
Advanced bioinformatics reporting is readily available on an per hour basis that consists of
gene set enrichment, paths, functional genomics, modeling, and publication ready output.
Human Immune System