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Centrifugation
Contents 1. Definition 2. Classification 3. Composition 4. Relative force & application
Centrifugation ,[object Object],[object Object],[object Object]
Classification  75000rpm 20000~25000rpm 3000rpm Ultra- centrifuge High speed centrifuge Desk top centrifuge
Desk top clinical centrifuges ,[object Object],[object Object],[object Object],[object Object]
High-speed centrifuges ,[object Object],[object Object],Refrigerated high-speed centrifuge Continuous flow centrifuge High speed centrifuges
Continuous flow centrifuge ,[object Object],[object Object],[object Object]
Refrigerated high-speed centrifuge ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Refrigerated high-speed centrifuge
The ultracentrifuge ,[object Object],[object Object],[object Object],[object Object],[object Object]
Composition ,[object Object],[object Object],[object Object],[object Object],[object Object]
Drive & Speed control ,[object Object],[object Object],[object Object],[object Object]
Overspeed system ,[object Object],[object Object],[object Object],[object Object],[object Object]
Temperature control ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Vacuum system ,[object Object],[object Object],[object Object]
Rotors ,[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object]
Relative centrifugal force  ,[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object]
Example ,[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
Application ,[object Object],[object Object]
Sedimentation velocity ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Sedimentation coefficient ,[object Object],[object Object],[object Object],[object Object]
Frictional coefficient ,[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Time  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
The density gradient ,[object Object],[object Object],[object Object],[object Object]
Example^ ,[object Object],at a high speed (about 40,000 rpm) for several hours
 
Sedimentation velocity Sedimentation equilibrium synonym Zone centrifugation Isopycnic , equilibrium density-gradient centrifugation gradient Shallow, stabilizing – maximum gradient density below that of least dense sedimenting species Steep – maximum gradient density greater than that of most dense sedimenting species centrifugation Incomplete  sedimentation , Short time , Low speed Complete sedimentation to equilibrium position, Prolonged time ,  High speed
Sedimentation velocity ,[object Object],[object Object],[object Object],[object Object]
 
Sedimentation equilibrium ,[object Object],[object Object],[object Object],[object Object],[object Object]
SUN WEI Pharmacy of woosuk university [email_address] Thank You !

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Centrifugation sunwei 2011.3.17

  • 2. Contents 1. Definition 2. Classification 3. Composition 4. Relative force & application
  • 3.
  • 4. Classification 75000rpm 20000~25000rpm 3000rpm Ultra- centrifuge High speed centrifuge Desk top centrifuge
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  • 32. Sedimentation velocity Sedimentation equilibrium synonym Zone centrifugation Isopycnic , equilibrium density-gradient centrifugation gradient Shallow, stabilizing – maximum gradient density below that of least dense sedimenting species Steep – maximum gradient density greater than that of most dense sedimenting species centrifugation Incomplete sedimentation , Short time , Low speed Complete sedimentation to equilibrium position, Prolonged time , High speed
  • 33.
  • 34.  
  • 35.
  • 36. SUN WEI Pharmacy of woosuk university [email_address] Thank You !