Fruit variety registration and variety release procedures

22nd June, 2021
“Varietal Release and
Registration in Fruit Crops”
Dr M. K. Verma
Ph.D., ARS (1995), FHSI, FISNS, FSHD, FISHD
Principal Scientist (Hort.)
Division of Fruits and Horticultural Technology
ICAR-Indian Agricultural Research Institute,
New Delhi, India
Email: mahenicar10@gmail.com
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Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
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Institute,
New
Delhi
-
110012

Objectives
• Procedure to release a fruit crop variety.
• Procedure to register a fruit crop variety.
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• Genetic resource in Horticultural crops
include germplasm collection of
– 2194 accessions of fruits,
– 11,229 accessions of vegetables,
– 10,184 accessions in tuber crops,
– 6336 accessions of spices,
– 1304 accessions in plantation crops,
– 1327 accessions in medicinal and aromatic
plants.
(comes under the domain of National
Biodiversity Authority)
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Fruits
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Horticultural
Technology
ICAR-
Indian
Agricultural
Research
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Delhi
-
110012

Agencies involved in the process of variety
release and registration
1. Agencies for variety release
– Institute/ University (IVRC, IVIC)
– SVRC
– CVRC
2. Agencies for variety registration
– NBPGR (IC No./ EC No.)
– PPV FRA
3. Agencies for variety notification
– DAC, MoA & FW
4. Other Agencies
– AICRP (Fruits, Arid Fruits, Temperate Fruits)
– NAGC
Division
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Agricultural
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Mechanism
• To meet the need of ever-increasing population, the breeders are
continuously making the efforts by developing the new varieties.
• Registration: To recognize the contribution of
breeders/researchers, ICAR operates the mechanism for
identification and recommendation for release of new crop varieties.
• Release: The DAC, MoA & FW, provides the mechanism for
notification of released varieties by Central Sub-Committee on Crop
Standards, Notification and Release of Varieties (CVRC) of
agricultural crops.
• Protection: Protection of Plant Varieties and Farmers’ Rights Act
(2001) ensures the protection of the IPRs of breeders and farmers
involved in developing the new varieties to be notified and released
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
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New
Delhi
-
110012

Registration
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
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New
Delhi
-
110012

• To take care of the ownership through
Registration of the varieties the
Protection of Plant Varieties and Farmer’s
Rights Authority was established on
11thNovember 2005
• by the Central Government
• vide Gazette Notification No. S.O. 1589(E).
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Germplasm registration at NBPGR
Source: Tyagi and Tak, 2012. IJAS, 82(8): 651-9
Division
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ICAR-
Indian
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Variety Release Procedure
• Levels
– Institute level (IVRC, IVIC): Chairman:
Director/ Jt. Director (Research)
– Sate Level: State Seed Sub-Committee on
Release of Crop Varieties (Chairman:
Development Commissioner/ Financial
Commissioner).
– Central level: Central Sub-Committee on Crop
Standards, Notification and Release of
Varieties for Horticultural Crops (Chairman,
DDG, Horticulture).
– Apex Level: PPV & FRA (Chairman: Registrar)
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Institute Level Variety Release Committee
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Institute level Proposal
Recently proposed proposal at IARI, New
Delhi
On
PUSA SWRNIKA GRAPE
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VARIETY IDENTIFICATION PROPOSAL OF
GRAPE HYBRID (Vitis vinifera L.)
Proposed by
Dr S.K. Singh
Division of Fruits & Horticultural Technology
ICAR - Indian Agricultural Research Institute
New Delhi - 110012
GRAPE (Vitis vinifera L.): Pusa Swarnika (Hy. 76-1, Hur x Cardinal)
Division
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Justification of Release
• There are very few commercial varieties of grape
growing under sub-tropical climate of north India.
• These suffers with occurrence of shot berries,
compact bunch with moderate fruit quality traits
and maturity coincides with pre-monsoon showers
results in berry cracking due to delicate berries.
• Therefore, it was demand of farmers/growers to
have a better variety with traits of:-
– Early maturing of berries,
– Loose bunch
– Bold berry
– Uniform berry size,
– Higher yield,
– Suitable for juice and Munakka purpose with
ideal TSS/acid ratio,
• The growers would like this variety (Pusa Purple
Seedless) because of its early maturity, seedless
berries, uniform berry size, higher yield, firm
texture, GA responsive etc.
Pusa Aditi
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Salient Features of Grape Hybrid
Hy. 76-1 (Pusa Swarnika)
• It is a unique grape hybrid having
– Early berry ripening habit,
– Loose bunch
– Golden yellow coloured bold berries,
– High TSS with good blend of TSS and acid ratio.
• Early maturing (75-78 DAFB) and suitable for
subtropical climate.
• Bunches loose and medium to large in size
(317 g).
• Berries are bold, large in size (4.14 g), round
golden-yellow colour, seeded with firm pulp.
High TSS (20.71°Brix), mild acidity and ideal
TSS/acid ratio.
• Moderately rich in nutraceutical properties
like total phenolics (91.29 mg/100 g), total
flavonoids (91.80 mg/100 g), and antioxidant
properties assessed by DPPH, FRAP and
CUPRAC assay.
• Suitable for table purpose, juice making and
Munnakka preparation.
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Agricultural
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TRIAL RESULTS OF GRAPE HY. 76-1
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Table 1a. Comparison of grape Hybrid 76-1 (Hur x Cardinal) with
‘Perlette’ a early-maturing commercial cultivar of North India.
Parameter Pusa Swarnika
(Candidate variety)
Perlette
(Check
variety)
%
advantage
Bearing behaviour Basal bearing Basal bearing -
Vine vigour Moderate Moderate -
Av. bunch wt. (g) 317.25 300.25 5.7
Av. berry wt. (g) 4.14 1.82 127.5
Seed no. Seeded Seedless -
Bunch length (cm) 18.25 18.05 1.10
Bunch width (cm) 13.90 10.65 30.52
Bunch compactness Very loose Very
compact
Extremely
good
Berry length (mm) 19.86 12.99 52.89
Berry diameter (mm) 18.25 12.70 43.70
TSS (0Brix) 20.71 18.88 9.70
Acidity (%) 0.63 0.64 -
TSS/Acid ratio 33.17 29.50 12.44
pH 3.68 4.10 -

TRIAL RESULTS OF GRAPE HY. 76-1
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Table 1b. Comparison of grape Hybrid 76-1 (Hur x Cardinal) with
‘Perlette’ a early-maturing commercial cultivar of North India.
Parameter Pusa Swarnika
(Candidate variety)
Perlette
(Check variety)
%
advantage
Vitamin C (mg/100 ml juice) 6.20 6.24 -
Total Phenolics (GAE, mg 100 g-1) 91.29 147.00 (-) 37.90
Total Flavonoids (QE, mg 100 g-1) 91.80 36.25 153.24
DPPH AOX activity (TE, µmol g-1) 7.11 5.80 22.59
CUPRAC AOX activity (µmol TE g-1) 1.08 2.13 (-) 49.30
FRAP AOX activity (TE, µmol g-1) 2.14 2.13 0.50
ABTS AOX activity (TE, µmol g-1) 3.15 1.56 101.92
Juice recovery (%) 66.33 68.24 (-) 2.80
Date of bud burst 3rd week Feb 4th Week Feb -
Date of full bloom 4th week March 3rd week March -
Date of harvest
(days after full bloom)
2nd week June 1st week June -
Days to maturity 76 71 -
Yield (MT/ha) on Bower system (3.0 m x
3.0 m)
16.71 14.50 15.24

Table 2a. Average physico-chemical parameters of hybrid 76-1 during last five
years (2015-19)
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Parameters Year Mean
2015 2016 2017 2018 2019
Av. bunch wt. (g) 327.00 306.00 352.00 256.00 345.2
5
317.25
Av. berry wt. (g) 4.50 4.20 4.00 3.80 4.20 4.14
Seed No. 2.00 3.00 3.00 2.00 3.00 2.60
Bunch length (cm) 19.00 20.00 15.00 19.00 18.25 18.25
Bunch width (cm) 14.00 13.00 15.00 14.00 13.50 13.90
Bunch compactness Very
loose
Very
loose
Very
loose
Very
loose
Very
loose
Very
loose
Berry length (mm) 19.50 19.00 20.39 19.92 20.50 19.86
Berry diameter (mm) 17.21 18.68 19.16 17.93 18.25 18.25
TSS (0Brix) 20.00 21.20 20.15 21.40 20.80 20.71
Acidity (%) 0.60 0.70 0.56 0.60 0.68 0.63
TSS/Acid Ratio 33.33 30.28 35.98 35.66 30.58 33.17
pH 3.70 3.88 3.61 3.61 3.62 3.68

Table 2. Average physico-chemical parameters of hybrid 76-1 during last five
years (2015-19)
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Parameters Year Mean
2015 2016 2017 2018 2019
Vitamin C (mg 100 ml -1 juice) 6.44 6.54 6.45 6.33 5.24 6.20
Total monomeric anthocyanins
(C3GE, mg kg-1)
0.00 0.00 0.00 0.00 0.00 0.00
Total Phenolics (GAE, mg 100 g-1) 92.75 93.22 93.70 91.32 85.45 91.29
Total Flavonoids (QE, mg /100 g-1) 92.40 92.67 91.74 92.79 89.42 91.80
DPPH AOX activity (TE, µmol g-1) 7.14 7.20 7.14 7.07 7.00 7.11
CUPRAC AOX activity (µmol TE
g-1)
1.05 1.10 1.03 1.08 1.15 1.08
FRAPAOX activity (TE, µmol g-1) 2.05 2.01 2.06 2.08 2.51 2.14
ABTS AOX activity (TE, µmol g-1) 2.95 3.20 3.02 3.06 3.50 3.15
Juice recovery (%) 65.24 64.25 68.45 67.24 66.45 66.33
Date of bud burst 20-22 Feb 18-20 Feb 19-20 Feb 18-20
Feb
17-20
Feb
3rd
week
Feb
Date of full bloom 25-28
March
24-26
March
25-28
March
28-30
March
26-28
March
4th
week
March
Date of harvest
(days after full bloom)
8-10 June 10-12 June 8-11 June 10-13
June
9-12
June
2nd
week
June
Days to maturity 75-77 77-79 75-78 74-77 75-78 76
Yield (MT/ha) on Bower System
(3.0 x 3.0 m)
16.52 17.50 15.80 17.50 16.24 16.71

Disease severity index and disease reaction to powdery mildew in
natural conditions as well as in artificial inoculation of leaves
Parameters Pusa Swarnika
(Hyb. 76-1)
Perlette
(Check)
DSI under natural conditions 52.25% 79.75%
Disease reaction under natural
conditions
Moderately
susceptible
Highly
Susceptible
DSI under artificial conditions 54.44 84.15
Disease reaction under artificial
conditions
Moderately
susceptible
Highly
Susceptible
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Disease severity index and disease reaction to
anthracnose in natural conditions as well as in artificial
inoculation of leaves
Parameters Pusa
Swarnika
(Hyb. 76-1)
Perlette
(Check)
DSI under natural conditions 41.70% 83.78%
Disease reaction under natural
conditions
Moderately
susceptible
Extremely Susceptible
Culture filtrate concentration
under ex situ condition ((1:1)
+++ +++
++ +++
+ ++
+ ++
+++ = necrosis over 3 mm from wounded spot, ++ =
necrosis over 2-3 mm from wounded spot, + = necrosis
over wounded spot, + = slight necrosis - = no necrosis
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Fig. 1. Gel Image of profile of Pusa Swarnika (Hyb. 76-1) and its parents ‘Hur’ and Cardinal
using SSR marker VVIh54.
Fig. 2. Gel Image profile of Pusa Swarnika (Hyb. 76-1) and its parents ‘Hur’ and ‘Cardinal’
using SSR marker VVMD24.

Plate 1. A fully mature vine laden with bunches trained on
Bower system
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Grape (Pusa Swarnika) vineyard on
Bower system
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Breeders
1. Late Dr. P.C. Jindal, Ex. Principal Scientist.
2. Sh. Kashmir Singh (Retd.), Ex. Principal Scientist.
3. Dr S.K. Singh, Head and Principal Scientist,
4. Dr M.K. Verma, Principal Scientist,
5. Dr V.B. Patel, Principal Scientist
6. Dr Jai Prakash, Principal Scientist
7. Late Dr Laxman Singh, Ex. Technical Officer
8. Sh. Surendra Pal (Retd.), Ex. Technical Officer
9. Dr Parimal Sinha, Principal Scientist, Plant
Pathology
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
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New
Delhi
-
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State Level Proposal
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Variety Release Proposal for
Delhi State Seed Sub Committee for Agricultural and Horticultural
Crops
Proposed by
Dr S.K. Singh
Division of Fruits & Horticultural Technology
ICAR - Indian Agricultural Research Institute
New Delhi - 110012
Grape: Pusa Aditi
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and
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Justification of Release
• There was very few varieties of grape i.e.,
predominantly ‘Perlette’ growing under
subtropical climate of north India. ‘Perlette’
suffers with occurrence of shot berries,
compact bunch with moderate fruit quality
traits and maturity coincides with
premonsoon showers results in berry
cracking due to delicate berries. Therefore,
therefore, it was demand of
farmers/growers to have a better variety
with traits of:-
• Early maturing,
• Uniform berry size,
• Higher yield
• Firm texture
• Moderate bunch texture
• Bold berries
• The growers would like this variety (Pusa
Aditi) because of its uniform berry size,
early maturity, higher yield, firm texture,
GA responsive etc.
Perlette
Pusa Aditi
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Salient Features of Grape Variety
(BA x Perlette) Hy. 75-32 (Pusa Aditi)
• It is a unique hybrid having early
maturing habit (80-85 DAFB) , large
bunch (440 g), large berry (2.8 g),
round yellowish-green in colour, firm
pulp (6N), good TSS (19.3oBrix), higher
yield (12.5 t/ha). Bunches free from
shot berries and compactness.
• The vine is moderately vigorous, and
is a spur pruned variety.
• The hybrid has good traits for making
juice and is best for table purpose.
• The hybrid is moderately tolerant to
anthracnose and powdery mildew.
Division
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Technology
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Indian
Agricultural
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110012

Grape vine of Pusa Aditi laden with bunches
Division
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Harvested bunches of Pusa Aditi
ICAR-
Indian
Agricultural
Research
Institute,

1. Name of the Crop and
species
Grape (Vitis vinifera L.)
2.a Name of the variety under
which tested
Hy. 75-32
b. Proposed name of the variety Pusa Aditi
3. Sponsored by Head, Division of Fruits and Horticultural
Technology, IARI, New Delhi-110012
4.a Division/Regional Station or
agency responsible for
developing variety (with
address)
Division of Fruits and Horticultural Technology,
IARI, New Delhi-110012
b Name of the persons who
helped in the development of
variety
1. Late Dr. P.C. Jindal, Ex. Principal Scientist.
2.Sh. Kashmir Singh (Retd.), Ex. Principal
Scientist.
3. Dr S.K. Singh, Head and Principal Scientist,
4. Dr M.K. Verma, Principal Scientist,
5. Dr V.B. Patel, Ex. Scientist
6. Dr Jai Prakash, Sr. Scientist
7. Late Dr Laxman Singh, Ex. Technical Officer
8. Sh. Surendra Pal (Retd.), Ex. Technical Officer
9. Dr Parimal Sinha, Principal Scientist, Plant
Pathology

5.a Parentage with details of its pedigree
It is a hybrid between ‘Banqui Abyad’ x ‘Perlette’
from the cross made in 1975.
b Source of material in case of
introduction
NA
c Breeding method The hybrid was made through conventional
crossing made between ‘Banqui Abyad’ and
‘Perlette’ and individual plant was further
propagated vegetatively for multiplication.
d Breeding objectives To develop high yielding, early maturing and
seedless variety for subtropical climate with
superior fruit quality traits.
e
Plant height Semi-vigorous, suitable for both head and bower
systems of training.
Distinguishing morphological
characters
1. Vegetative characters
2.Flower characters
3.Fruit characters
Vines are perennial, semi-vigorous, produces
profuse vegetative and reproductive wood.
Hermaphrodite; self fruitful, panicles emerge 20-25
days after bud burst.
Elongated bunch (440 g), yellowish seedless berry
suitable for table grape. Responsive to GA3 and
matures by 1st week of June (before onset of
monsoon). TSS 19.3 0Brix.

Table 1. Comparison of grape Hybrid BA x Per-75-32 with ‘Perlette’ a
commercial cultivar of north India
Sl. No. Characteristics Hybrid BA x Per-75-32 Perlette
1. Bearing of behavior Basal bearing Basal bearing
2. Vine vigour Moderate Moderate
3. Av. Berry weight (g) 2.77 1.81
4. Av Bunch weight (g) 440.14 410.6
5 Peel colour Yellowish-green Greenish-yellow
6 Reaction to Powdery
mildew
Moderately tolerant Susceptible
7 Reaction to Downy
mildew
8 Reaction to
Anthracnose
9 Reaction to termite Moderately tolerant Highly susceptible
10 Average yield (kg/vine)
on Head system
10-13 7-8
11. No. of bunches per
plant (Head system)
31.67 18.45
12 TSS (0Brix) 19.31 18.23
13. Acidity (%) 0.56 0.44
14 Date of bud burst 22th Feb 21st Feb
15 Date of full bloom 19rd March 17th March
16 Date of harvest 10th June 2nd June
17 Days to maturity 80-85 75-80 days

Table 2. Average physico-chemical parameters of hybrid BA x
Per-75-32 for five years (2012-16)
Character Year Mean
‘Pusa
Aditi’
Mean for
‘Perlette’
5 yrs
CD at 5%
2012 2013 2014 2015 2016
Av. bunch wt.
(g)
524.69 481.76 505.27 313.75 375.2 440.14 410.6 NS
Av. berry wt. (g) 3.02 2.70 2.75 2.72 2.68 2.77 1.81 0.21
Bunch length
(cm)
22.15 23.80 24.52 19.6 21.11 22.24 18.51 1.60
Bunch width
(cm)
14.12 11.30 12.11 12.6 16.45 13.32 16.78 1.69
Bunch volume
(ml)
459 460 472 268 351 402 399 NS
Berry length
(mm)
15.21 15.99 15.87 15.5 15.44 15.60 13.21 0.36
Berry diameter
(mm)
16.97 16.12 16.56 15.1 16.78 16.31 13.32 0.57
TSS (0Brix) 20.12 19.60 18.72 18.6 19.51 19.31 18.23 0.90
Acidity (%) 0.49 0.66 0.62 0.49 0.54 0.56 0.44 0.10
Juice (%) 64.25 64.85 65.25 65.68 66.45 65.30 69.42 1.22
Berry firmness
(N)
6.45 6.12 5.32 6.33 5.76 6.00 4.23 0.37
Date of bud
burst
21.2.12 23.2.13 22.2.14 23.2.15 20.2.16 25th Feb - -
Date of full
bloom
19.3.12 20.3.13 18.3.14 19.3.15 18.3.16 23rd
March
- -
Date of harvest 10.6.12 9.6.13 9.6.14 8.6.15 11.6.16 10th June - -
Days to maturity 84 83 83 84 85 83 80 3.44

Pusa Aditi
Perlette
Division
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Central Level Proposal
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

Division
of
Fruits
and
Horticultural
Technology
Proposals for Release of Fruit Varieties
to the Central Sub-Committee on Crop
Standards, Notification and Release of
Varieties
ICAR- Indian Agricultural
Research Institute, New Delhi

Division
of
Fruits
and
Horticultural
Technology
Mango
Pusa
Pratibha
Pusa Shreshth Pusa Lalima Pusa Peetamber

Division
of
Fruits
and
Horticultural
Technology VARIETY RELEASE PROPOSAL
Pusa Shreshth (H 1-6) (Amrapali x Sensation)
Name of the crop
with Latin name
Mango (Mangifera indicia L.)
Parents ♀ Amrapali x ♂ Sensation
Year of cross 1994
Breeding method Hybridization followed by Selection
Year of planting 1996
Hybrid code Hybrid H-1-6
National identity IC634475
State Variety
Release Committee
22.07.2011 Vide-No.F.10/SI/TA/2011-12/PO/2534
Dated 17.02.2012
Breeder’s/
Contributors
Dr. S. N. Pandey, Principal Scientist (Retd.); Late Dr.
Room Singh, Principal Scientist (Retd.); Sh. S. K.
Bhagat, Scientist(SG) (Retd.); Dr. R. R. Sharma,
Principal Scientist; Dr. A. K. Singh, DDG (Hort. Sci.,
ICAR, Former Head & Principal Scientist; Dr. A. K.
Dubey, Principal Scientist; Dr. Manish Srivastav,
Principal Scientist .

Division
of
Fruits
and
Horticultural
Technology Pusa Shreshth (Amrapali x Sensation)
Salient features
• Trees are semi-vigorous and suitable for medium density planting
(6 m x 6 m = 278 trees per hectare).
• Regular bearing habit.
• Suitable for export as well as domestic markets.
• On per tree basis, it yields 50.2 kg. Estimated yield is 14.0 t/ha.
• Fruits are elongated in shape and uniform in size.
• Fruits are having attractive red purple peel colour on yellow
background, which makes it very appealing to the buyers.
• It is medium maturing (140 days from flowering).
• Suitable for North, Central and Peninsular India.
• Fruits are medium in size (230-250 g) with high pulp content
(71.88%), moderate total soluble solids (20.33oBrix), ascorbic acid
content (40.26 mg/100 g pulp) and β-carotene content (10,857 µg/
100 g of pulp). Shelf-life is of 7-8 days at room temperature.

Division
of
Fruits
and
Horticultural
Technology Parentage details and flow chart of
development of Pusa Shreshth
Progenies evaluated
during 2001-2008
Released by SVRC 2011
1994
1996
Selection

Division
of
Fruits
and
Horticultural
Technology Molecular profiling of Pusa Shreshth along
with parents
M Amrapali Sensation Pusa Pratibha
M A S Pusa Pratibha
SSR profile of Pusa Shreshth and its parents Amrapali and Sensation
with SSR markers M73, M109 and M178.
Mango SSR M178
M A S Pusa Shreshth
100 bp
200 bp
300 bp
100 bp
200 bp
300 bp
Mango SSR M73
M Amrapali Sensation Pusa Shreshth
Mango SSR M109
M Amrapali Sensation Pusa Shreshth

Division
of
Fruits
and
Horticultural
Technology Detailed description of Pusa Shreshth and parents
M A S Pusa Pratibha
S.N. Trait Pusa Shreshth
(H-1-6)
Amrapali Sensation
1. Bearing behaviour Regular Regular Regular
2. Tree vigour Semi-vigorous Dwarf Vigorous
3. Recommended spacing (m) 6 x 6 5 x 5 10 x 10
4. No. of plants / ha 278 400 100
5. Average fruit weight (g) 228 165 186
6. Average yield tree (kg/ tree) 50.2 37.5 82.6
7. Average yield per ha (t/ ha) 14.0 15.0 8.26
8. Peel colour Attractive red purple
colour on yellow
background
Green Red/ purple
9. Peel thickness Medium Thin Thick
10. Reaction to major mango
pests like hoppers, mealy
bugs etc.
Moderately tolerant Moderately
susceptible
Susceptible
11. TSS (%) 20.3 22.5 16.5
12. Pulp (%) 71.8 72.4 60.2
13. Fiber in pulp Fibreless Fibreless Slight fiber
14. β-carotene (µg/100 g pulp) 10,964 14,615 6,453
15. Acidity (%) 0.21 0.12 0.38
16. Ascorbic acid content
(mg/100 g pulp)
40.26 30.23 35.7
17. Shelf-life (days) 7 to 8 7 to 8 9.66

Division
of
Fruits
and
Horticultural
Technology Yield data of Pusa Shreshth
M A S Pusa Pratibha
Trait
Year Mean
2001 2002 2003 2004 2005 2006 2007 2008
Average fruit weight (g) 230 240 232 241 192 236 211 237 228.2
Pulp (%) 71.5 71.5 72.5 72.6 71.8 72.1 70.9 71.7 71.8
TSS (%) 19.6 19.5 18.5 19.7 21.3 21.6 20.8 21.6 20.3
Acidity (%) 0.24 0.24 0.25 0.27 0.15 0.14 0.15 0.20 0.205
β- carotene (µg/100 g
pulp)
12,200 12,600 13,200 12,485 9,510 8,375 8,456 10,883 10,964
Ascorbic acid content
(mg/100 g pulp)
40.5 40.0 42.1 38.79 39.44 40.53 41.2 39.55 40.26
Trait
Year Mean
2016 2017 2018 2019 2020
Fruit weight (g) 206.3 222.2 212.4 220.9 205.9 213.54
Pulp (%) 69.3 71.6 68.9 69.5 70.4 69.94
TSS (%) 20.5 19.8 19.6 19.7 20.2 19.96
Acidity (%) 0.24 0.23 0.26 0.18 0.20 0.222
β- carotene (µg/100g pulp) 9,887 11,008 10,986 11,239 11,165 10,857
Ascorbic acid content (mg/100 g pulp) 42.1 42.6 45.4 39.6 42.4 42.42
Shelf-life (days) after ripening 7.33 7.06 6.60 7.00 6.33 6.80
Pre-release
Post-release
AICRP center Fruit weight (g)
Sabour, Bihar 260
Pantnagar, UK 215
PAU, Ludhiana 240
Farmer/ Govt. Nursery Fruit weight (g)
Sri PD Singh, Srawasti 310
Sri DK Sharma, Bijnore 225
Sri Harish Kumar, Bijnore 280
Dr Brijesh Gupta, Kashipur 260

Division
of
Fruits
and
Horticultural
Technology Pusa Shreshth
M A S Pusa Pratibha
Pusa Shreshth Tree (10 years) New & mature leaf Ripe fruits
Tree ripe fruit colour Ripe fruits pulp colour

Sri Harish Kumar, Bijnour, Uttar Pradesh
Performance at Farmer’s Field

Division
of
Fruits
and
Horticultural
Technology
Citrus
Acid lime: Pusa Abhinav Sweet orange: Pusa
Round

Salient features of acid lime cultivar ‘Pusa
Abhinav’ (Accession No. IC-0611371)
• Improved clonal selection for commercial cultivation in
subtropical region of India
• Distinct advantage in harvesting season, yield and quality
characters over available commercial lime cultivars
• Yield and fruiting density are 60% and 208% higher than
check
• Average fruit weight- 39.1 g; juice recovery- 47.9%; TSS-7.90B
and ascorbic acid content 43.9 mg/100 ml juice.
• Higher acidity (6.9%) than check (5.81%).
Breeders: Dr. A. K. Dubey, Principal Scientist , Dr. Manish Srivastav, Principal
Scientist, Dr R. M. Sharma, Principal Scientist, Dr. O. P. Awasthi, Principal Scientist,
Dr. M. K. Verma, Principal Scientist , Dr. (Mrs.) Charanjit Kaur, Principal Scientist, Dr
Dinesh Singh, Principal Scientist

Mean performance of acid lime cultivar ‘Pusa Abhinav’
and check variety from 2015-2019 at I.A.R.I, New Delhi
Variety Pusa Abhinav
(AlC-40)*
Purbi lime (Local
Check)
Per cent
change over
check
Yield/tree (kg) 20.69 12.87 60.76
Fruiting density (Fruits/m3 CV) 4.84 1.57 208.28
Fruit weight (g) 39.1 36.65 6.66
Juice recovery (%) 47.9 36.82 30.06
Seed number/fruit 8.4 9.56 -12.55
Rind thickness (mm) 1.0 1.46 -32.47
TSS (0B) 7.9 7.86 -0.13
Acidity (% citric acid) 6.9 5.81 18.94
Ascorbic acid (mg/100 g juice) 43.9 33.45 31.30
*yields twice in a year but included yield of one season (rainy season), because
check bears once in a year.

Performance of acid lime variety ‘Pusa Abhinav’ at
different farmer’s field (planted during 2017-2019).
Plant height (m)
Genotype Pusa Abhinav Pusa Udit Local type
Hissar (Haryana) 1.45 1.33 2.56
Sonipat (Haryana) 1.56 1.75 4.23
Etawah (UP) 2.30 2.45 3.4
Bijnor (UP) 3.1 3.5 -
Gautam Budh Nagar UP) 3.1 3.3 -
Fruit/tree
Genotype Pusa Abhinav Pusa Udit Local type
Hissar (Haryana) - - -
Sonipat (Haryana) 256 211 -
Etawah (UP) 65 54 -
Bijnor (UP) - - -
Gautam Budh Nagar UP) 350 365 -

Description of acid lime cultivar ‘Pusa Abhinav’
S. No Character Description
01 Tree growth habit Spreading
02 Canopy dense
03 Spine: Density of spines on the adult tree
(one year old shoot 30 cm
Medium and dense (<10 nos/30 cm)
04 Leaf
Shape of leaf blade Elliptic
Shape of leaf apex Acute
Shape of leaf margin Denate
Shape of leaf base Obtuse
04 Fruit character
Fruit shape Round to ovoid
Fruit surface colour at ripening Yellow
Fruit core Solid
Neck Absent
Fruit surface texture Smooth
Juice colour yellow Light yellow
Fruit juiciness High
05 Seed per fruit (<10) Low seeded
06 Fruit quality
Acidity (>6.5% citric acid) High
07 Bearing habit Twice in a year under subtropical
semi-arid conditions

Bearing tree of Pusa Abhinav
Fruits of Pusa Abhinav

SALIENT FEATURES OF SWEET ORANGE SELECTION
Pusa Round (MS-13) (Accession No. 274693)
• Improved selection for commercial cultivation under
Subtropical conditions as well lower mid hills of
Uttarakhand
• Distinct advantage in yield and quality characters over
available commercial sweet orange cultivars
Average fruit weight- 242.18 g; juice content-48.47%;
Yield/tree-3.78-fold higher and yield efficiency (kg/m3
CV)-1.69-fold higher than Jaffa (check).
• High TSS (12.000B) with moderate acidity (1.00%).
• Early maturity (15th October).
Breeders: Dr A. K. Dubey, Principal Scientist, Dr Manish Srivastav, Principal
Scientist, Dr O. P. Awasthi, Principal Scientist, Dr M. K. Verma, Principal
Scientist, Dr (Mrs.) Charanjit Kaur, Principal Scientist

Average performance of MS-13 (Pusa Round) during
2012-13 to 2016-17 at ICAR-IARI, New Delhi against check
Variety Pusa Round
(MS-13)
Jaffa
(Local Check)
Per cent change
Yield (kg/tree) 34.20 9.03 278.74
Yield Efficiency ((kg/ m3 CV) 0.56 0.33 69.70
Fruiting Density (Fruits/m3
CV) 2.88 2.69 7.06
Fruit weight (g) 242.18 152.44 58.87
Juice (%) 48.47 50.64 -4.29
Seeds/fruit 22.16 9.91 123.61
Peel thickness (mm) 3.99 2.93 36.18
TSS (0B) 11.98 8.07 48.45
Acidity (% citric acid) 1.00 0.67 49.25
Ascorbic Acid (mg/100 ml
juice) 43.74 37.85 15.56

Performance of sweet orange cultivar Pusa Round (MS-13)
at Farmer’s field
Genotype Plant height (m)
Pusa Round MBR/ Mosambi
/Malta
Pataudi (Haryana) 3.25 3.56
Sonipat (Haryana)* 4.12 3.56
Gautam Budh Nagar UP)* 3.79 4.12
Fruit/tree
Genotype Pusa Round MBR/ Mosambi
/Malta
Pataudi (Haryana) 156 75
Sonipat (Haryana)* 205 225
Gautam Budh Nagar UP)* 135 85
*Tree age: 4 Years

Description of sweet orange cultivar ‘Pusa
Round’ (MS-13)
Characters Pusa Round (MS-13)
Fruit shape Round
Fruit core Semi solid
Fruit surface texture Rough
Fruit Weight (g) >240 g
Fruit diameter (mm) >75
Fruit length (mm) >75
Shape of fruit apex Flat
Fruit rind (epicarp) colour Light Orange
Fruit rind thickness (mm) >4
Fruit juiciness (%) High
Juice colour Yellow
Titratable acidity (% citric acid) <1.0
Remark Early maturity

Bearing tree of Pusa Round
Fruits of Pusa Round
Fruits of Pusa Round

Out of these 26 primers, TAA45 able to distinguish among
sweet orange variety Malta clone
P1; Pusa Sharad, P2; MS-1, P3-; MS-2, P4; MS-3, P5; MS-
8, P6-Jaffa, P7: Pusa Round

Scion Bank Establishment
• SKUAST, Jammu
• IARI Regional Station, Karnal, Haryana
• PAU, Kudhiana, Punjab
• CAU, Jhansi, Uttar Pradesh
• NRC for Agro-forestry, Jhansi Uttar Pradesh
• CAU, Pashighat, Arunachal Pradesh
• Fruit Research Station, MPUAT, Sriganganagar,
Rajasthan.
• RVSUA, Gwalior, Madhya Pradesh
• Dr BSKKV, Dapoli, Maharashtra
• ICAR- CCRI, Nagpur, Maharashtra
• Fruit Research Station, Tirupati, Andhra Pradesh

Division
of
Fruits
and
Horticultural
Technology
Grape
Pusa Navrang Pusa Urvashi Pusa Aditi

Salient Features of ‘Pusa Navrang’
(Accession No. IC- 616658 )
• Developed through hybridization between ‘Madeleine Angevine’ and
‘Rubired’.
• Early maturing (90-95 days after full bloom) under sub-tropical
conditions of New Delhi.
• Highly fruitful and wider adaptability (temperate, tropical and
subtropical).
• Heavy cropping with medium berry size, seeded, suitable for juice
making.
• Medium size of bunch & berry dark purple in colour, tenturier (pulp
and peel both coloured), TSS 18-20°Brix, moderate acidity (0.60 to
0.72%), higher content of total monomeric anthocyanin contents
(791.04 to 900 C3GE, mg kg-1 of juice), total phenolics (284.25 GAE,
mg/100 g) and total flavonoids (127.75 QE, mg/100 g).
• Field resistance to anthracnose, powdery mildew and downy mildew.
• Average yield on trellis system is 14-15 t/ha under subtropical climate
of north India.
Division
of
Fruits
and
Horticultural
Technology

Comparison of ‘Pusa Navrang’ with check varieties (‘Punjab Purple’ and
‘Black Muscat’) based on pooled data of 5 years (2015-19).
Characteristics Pusa
Navrang
Punjab Purple
(Check 1)
Black Muscat
(Check 2)
Bunch wt. (g) 144.16 171.00 249.00
Berry wt. (g) 1.60 4.48 3.38
Berry length (mm) 13.77 13.65 16.62
Berry diameter (mm) 14.02 13.58 15.68
TSS (°Brix) 18.77 20.90 16.75
Total titratable acidity (%) 0.76 0.73 0.59
TSS/Acid Ratio 24.69 28.63 28.38
pH 3.95 3.90 3.60
Vitamin C (mg/100 ml juice) 3.56 6.24 6.24
Total monomeric
anthocyanins (C3GE, mg kg-
1)
824.88 576.21 52.77
Total Phenolics (GAE, mg
/100 g)
287.12 177.00 258.00
Juice recovery (%) 67.45 64.05 62.45
Date of bud burst 24-29 Feb 25-28 Feb, 24-29 Feb
Date of full bloom 14-18
March
15-18 March 15-20 March
Date of harvest 8-10 June 12-20 June 12-20 June
Days to maturity 92 100 102
Yield (t/ha) on Extended Y
(2.5 x 3.0 m)
15.30 12.0 13.5
Division
of
Fruits
and
Horticultural
Technology

Average yield (kg/vine)
Variety Yield per vine
(3 yr old)
Pusa Navrang 12.20 kg/vine
Arka Neelamani 4.70 kg/vine
Arka Krishna 0.43 kg/vine
Arka Chitra 0.95 kg/vine
Variety Yield (kg/vine)
Pusa Navrang 22.5
Cardinal 8.26
Paschim Midnapore, WB
Pusa Navrang 17.8 kg
Bangalore Blue 6.1
Cab. Savig. 4.2
Merlot 3.5
Gulabi x BB 13.4
Variety TSS%
Pusa
Navrang
18.60
Arka
Neelamani
20.2
Arka Krishna 18.60
Arka Chitra 18.6
Pusa
Navrang
64.6
Bangalore
Blue
57.9
Cab. Savign. 62.8
Merlot 55.40
Shiraz 62.6
Variety Juice %
Pusa
Navrang
69.10
Arka
Neelamani
70.0
Arka Krishna 64.9
Arka Chitra 64.5
Variety TSS%
Pusa
Navrang
16.05
Cardinal 16.65
Juice % TSS%
Paschim
Midnapore, WB
Paschim
Midnapore, WB
Lucknow, UP
Lucknow, UP
Hyderabad,
Telengana
Hyderabad, Telengana
Performance of ‘Pusa Navrang’ at different locations

Pusa Navrang
Points for
release
• Nutraceutically
rich.
• Highly tolerant
to pest and
diseases.
• High yielding
• Tolerant to
high
temperature
and rain
induced
cracking.
• Early maturing.
• Suitable for
juice and
coloured wine
making
Indents received and material
supplied:
1. Champhai, Mizoram
2. Jammu and Kashmir
3. Dehradun, Uttarakhand
4. Kota, Rajasthan
5. Ludhiana, Amritsar,
Bhatinda, Punjab
6. Hissar,
Mahendergarh,Haryana
supplied:
7. Koraput, Odisha
8. Bankura, Mednapore, WB
9. Mandsour, MP
10. Theni- Tamilnadu
11. Ratlam, MP
12. Muzaffarnagar, UP
supplied:
13. Raipur, Chhattisgarh
14. Meerut, UP
15. Lucknow, UP
16. Pune, Maharastra
Division
of
Fruits
and
Horticultural
Technology

Fig. 1. Gel Image of SSR marker VVIh54 for Pusa Navrang and parents
Fig. 2. Gel Image for SSR marker VVMD24 for Pusa Navrang and parents
MA
Rubired
MA
Rubired
Division
of
Fruits
and
Horticultural
Technology

Breeders
Developers
(Breeders)
1. Late Dr P.C. Jindal, Principal
Scientist, F&HT
2. Sh. Kashmir Singh, Scientist (SG)
(Retd.).
3. Dr Shyam Nagina Pandey, Former
ADG (Hort. Sci.), ICAR & Principal
Scientist, F&HT (Retd.)
Collaborators
name, who are
involved in
multiplication
and promoting
the variety
1. Dr S.K. Singh, Principal Scientist,
F&HT
2. Dr M.K. Verma, Principal
Scientist, F&HT
3. Dr V.B. Patel, Principal Scientist,
F&HT
4. Dr Chavlesh Kumar, Scientist,
F&HT
Division
of
Fruits
and
Horticultural
Technology

Salient Features of ‘Pusa Urvashi’
(Accession No. IC- 612129)
• It is a hybrid between ‘Hur’ x ‘Beauty Seedless’ released by
IARI in 1996.
• It is early maturing (97 days after full bloom), particularly
suitable for sub-tropical conditions.
• Bunches are semi-compact, large in size (326 g), uniformly
round, seedless berries (2.68 g), greenish-yellow in colour
and is GA responsive.
• Moderate TSS (17.34°Brix) and acidity with ideal TSS:acid
ratio.
• Moderately rich in nutraceutical properties like total
phenolics (54.79 mg/100 g), total flavonoids (35.25 mg/100
g), and moderate antioxidant properties assessed by DPPH,
FRAP and CUPRAC assay.
• Suitable for table purpose and juice making.
Division
of
Fruits
and
Horticultural
Technology

Comparison of ‘Pusa Urvashi’ with ‘Perlette’ a commercial
cultivar of north India based on pooled data of 5 years (2015-19).
Parameters Pusa Urvashi
(Candidate variety)
Perlette
(Check variety)
Vine vigour Moderate Moderate
Bunch wt. (g) 323.60 320.25
Berry wt. (g) 2.68 1.82
Peel colour Greenish-yellow Greenish-yellow
Number of seeds per berry Seedless Seedless
Berry diameter (mm) 15.55 12.70
TSS (°Brix) 17.34 18.88
Total titratable acidity (%) 0.70 0.64
TSS/Acid ratio 24.93 29.50
Vitamin C (mg/100 ml juice) 8.80 6.24
Juice recovery (%) 70.15 68.24
Reaction to Powdery mildew Moderately tolerant Susceptible
Reaction to Downy mildew Moderately tolerant Susceptible
Reaction to Anthracnose Moderately tolerant Susceptible
Date of harvest 2nd week June 1st week June
Days to maturity (days after full
bloom)
97 88
Yield (t/ha) on Bower (3.0 x 3.0 m) 20.60 14.50
Division
of
Fruits
and
Horticultural
Technology

Performance of ‘Pusa Urvashi’ at different
locations
Variety Yield
(kg/vine)
Juice (%) TSS %)
Perlette 0.58 76.1 19.8
Pusa
Urvashi
2.90 69.3 19.1
West Bengal, 3 yrs old vine
Variety Yield
(kg/vine)
Berry
weight (g)
TSS %)
Pusa
Urvashi
1.2-2.89 1.61 16.07
Perlette 2.69-9.84 1.52 17.37
New
Perlette
2.25-4.80 1.38 18.35
Lucknow, 3 yrs old vine
Division
of
Fruits
and
Horticultural
Technology

Description of ‘Pusa Urvashi’
Plant height /
Vine length
Moderately vigorous
Plant type
Leaf characters
Flower
characters
Fruit characters
Vines are semi-vigorous.
Medium leaf blade size, pentagonal in shape, 3 number of lobes,
self-fruitful, male and female flowers fully developed.
Bunch size large (326.6 g), berry size medium, berry shape
round, seedless, berry skin colour greenish-yellow, juicy flesh.
Responsive to GA3.
Maturity Ready for first harvesting in 95-100 days after full bloom.
Maturity Group Early maturing
Reaction to
major diseases
Moderately field tolerance against powdery mildew (Uncinula
necator), downy mildew (Plasmopara viticola) and anthracnose
(Elsinoë ampelina), the important diseases of grapes prevalent
in northern plains.
Division
of
Fruits
and
Horticultural
Technology

Pusa Urvashi
Points for
release
• Seedless.
• High yielding
• Early
maturing.
• High bud
fruitfulness
• Suitable for
table and
juice making
• Suitable for
sub-tropical
climate
Indents received and
material supplied:
2. Dehradun, Uttarakhand
3. Kota, Rajasthan
4. Punjab: Amritsar,
Bhatinda, Ludhiana
5. Haryana: Hissar,
Sonipat,
material supplied:
6. Bankura, W.B.
7. Mandsour, MP
material supplied:
14. Meerut, UP
15. Lucknow, UP
16. Pune, Maharashtra
Division
of
Fruits
and
Horticultural
Technology

Fig. 1. Gel Image of SSR marker VVIh54 for Pusa Urvashi and parents
Fig. 2. Gel Image for SSR marker VVMD24 for Pusa Urvashi and parents
Division
of
Fruits
and
Horticultural
Technology

Breeders
Developers 1. Late Dr P.C. Jindal, Principal Scientist,
F&HT
2. Sh. Kashmir Singh, Scientist (SG)
(Retd.).
3. Dr Shyam Nagina Pandey, Former ADG
(Hort. Sci.), ICAR & Principal Scientist,
F&HT (Retd.)
Collaborators
name, who are
involved in
multiplication
and promoting
the variety
Dr S.K. Singh, Principal Scientist, F&HT
Dr M.K. Verma, Principal Scientist, F&HT
Dr V.B. Patel, Principal Scientist, F&HT
Dr Chavlesh Kumar, Scientist, F&HT
Division
of
Fruits
and
Horticultural
Technology

Salient Features of ‘Pusa Aditi’
(Accession No. IC- 0628795)
• It is a hybrid between ‘Banqui Abyad’ and ‘Perlette’
released by the State Seed-Committee for Agricultural
and Horticultural Crops, Government of N.C.T. of Delhi
on 19th December, 2017.
• Early maturing (82-85 days after full bloom), suitable
for sub-tropical conditions, escape damage by pre-
monsoon showers.
• Bunches semi-compact, large in size (440 g),
uniformly round, seedless berries (2.77 g), greenish-
yellow in colour and is GA (30-45 ppm) responsive.
• Moderate TSS (19.31°Brix) and low acidity (0.56%).
• Suitable for table purpose and juice making.
Division
of
Fruits
and
Horticultural
Technology

Comparison of ‘Pusa Aditi’ with ‘Perlette’ a commercial cultivar on
pooled data of 5 years (2015-19).
Characteristics Pusa Aditi Perlette (Check)
Bearing of behavior Basal bearing Basal bearing
Vine vigour Moderate Moderate
Av. berry weight (g) 2.77 1.82
Av. bunch weight (g) 440.14 411.1
Peel colour Greenish-yellow Greenish-yellow
Reaction to Powdery
mildew
Reaction to Downy
mildew
Reaction to Anthracnose Moderately tolerant Susceptible
Reaction to termite Moderately tolerant Highly susceptible
Average yield (kg/vine) 10-12 (Head system)
17-18 (Bower system)
7-8 (Head system)
12-14 (Bower system)
TSS (0Brix) 19.31 17.88
Date of harvest 10th June 2nd June
Days to maturity 80-85 DAFB 75-80 DAFB
Division
of
Fruits
and
Horticultural
Technology

Performance of ‘Pusa Aditi’ at different
locations
Variety Yield
(kg/vine)
TSS
Perlette 0.58 17.34
Pusa
Aditi
1.69 16.17
CISH Lucknow, 5 yrs old
vine
Variety Yield (kg/vine)
Pusa
Aditi
15.50 kg/vine
Perlette 10.20 kg/vine
New
Perlette
2.25-4.80
Rajnandgaon, Chhatisgarh
Variety Yield
(kg/vine)
TSS
Pusa
Aditi
2.90 18.17
Perlette 0.58 19.8
Bankura, WB, 3 yrs old vine
Ratlam, Madhya Pradesh &
Kota, Rajasthan
Farmers feedback:
Early maturing grape varieties like
Perlette, Pusa Aditi, Pusa
Navrang, are doing well in the region

Description of ‘Pusa Aditi’
Plant height / Vine
length
Moderately vigorous
1.Plant type
2. Leaf characters
3.Flower
characters
4.Fruit characters
Vines are semi-vigorous.
Medium leaf blade size, pentagonal in shape, 5
number of lobes.
Hermaphrodite, self-fruitful, male and female
flowers fully developed, green in colour, and
medium in size.
Bunch size large (440 g), berry size medium, berry
shape round, seedless, berry peel colour green-
yellow, TSS 19.30Brix.
Maturity Ready for first harvesting in 82-85 days after full
bloom.
Maturity Group Early maturing
Reaction to major Moderately field tolerance against powdery
mildew, downy mildew and anthracnose, the
important diseases of grapes prevalent in northern
plains.
Division
of
Fruits
and
Horticultural
Technology

Pusa Aditi
Points for
release
• Seedless.
• Firm and bold
berries.
• High yielding
• Early maturing.
• High bud
fruitfulness
• Suitable for table
and juice making.
• Suitable for sub-
tropical climate
material supplied:
2. Uttarakhand
3. Kota
4. Punjab: Amritsar,
Bhatinda
5. Haryana: Hissar,
Sonipat,
material supplied:
6. Bankura, W.B.
7. Mandsour, MP
material supplied:
14. Meerut, UP
15. Lucknow, UP
16. Pune, Maharastra
Division
of
Fruits
and
Horticultural
Technology

Fig. 1. Gel Image of SSR marker VVIh54 for Pusa Aditi and parents
Fig. 2. Gel Image for SSR marker VVMD24 for Pusa Aditi and parents
Division
of
Fruits
and
Horticultural
Technology

Breeders
Developers 1. Late Dr P.C. Jindal, Principal Scientist,
F&HT
2. Sh. Kashmir Singh, Scientist (SG) (Retd.).
Collaborators
name, who are
involved in
multiplication and
promoting the
variety
1. Dr S.K. Singh, Principal Scientist, F&HT
2. Dr M.K. Verma, Principal Scientist, F&HT
3. Dr V.B. Patel, Principal Scientist, F&HT
4. Dr Jai Prakash, Principal Scientist, F&HT
5. Dr Jai Prakash, Principal Scientist
6.Dr Laxman Singh, Ex. ACTO (Expired)
8. Sh. Surendra Pal, Ex. ACTO (Retired)
Division
of
Fruits
and
Horticultural
Technology

Proceedings of CVRC/ Gazette Notification
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

Variety Protection
PPV & FRA
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

PPV&FR Authority
• The enactment and establishment of the
Authority was based on the sui generis
system adopted in harmonization with
UPOV system of Novelty, Distinctness,
Uniformity and Stability.
• Farmers’ Rights Section was an unique
feature of the Act, which gave the much
needed importance to the Farmers.
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

Objectives of the PPV&FR Authority
• To provide an effective system for protection
of Plant varieties and rights of farmers and
plant breeders
• To recognize the farmers in respect of their
contribution made at conserving , improving
and making available plant genetic resources
(in light of FAO and CBD Agenda 21) for
development of new plant varieties
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

• To protect plant breeders’ rights to
stimulate investment for R&D and
develop new varieties (essence of UPOV
convention)
• To facilitate the growth of seed industry
to ensure production and availability of
high quality seed/planting material
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

Implementation of PPV&FR Act 2001
• PPV&FR rules notified in 2003, Authority
established on 11th November, 2005, Regulations
notified in 2006.
• Publication of Plant Variety Journal of India
started from February-March 2007.
• General guidelines for DUS testing and Crop
specific DUS test guidelines for 12 crops were
published and released on 20th February, 2007
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

Process of registration
Development of DUS Guideline
Gazette Notification
Application by Breeder/Farmers
Application Accepted/Rejected
DUS Test for accepted entry
+ DUS test Fee
Analysis of DUS data
Acceptance/Rejection for claim
For Novelty & DUS
Registration Grant in
National Plant Variety Register
Marketing of Registered variety
Internal Scrutiny PV 1, TQ, NORV,
IINDUS
Seed Deposit (National Gene Bank)
Two independent growing season
Test for Special characters
Payment of Registration Fee
Annual/Renewal Fee during
Protection period24
Compulsory Licensing/Benefit
sharing /denomination alter
Surrender/Revocation of
Registration

Fees payable Amount of Fee Form
No
Conducting Tests Max 35,000/-per entry -----
Notice of oppositions Rs 1500/- PV 3
Extension of Time Rs 1500/-per month PV 5
Fees for registration of EDVs Individual -5000/-
Educational –7000/-
Commercial–10000/- ---
Renewal Fee as above PV 6
Application for Benefit Sharing Rs 5000/- PV 7
Application for Registering as Agent/License Rs10000/- PV 9
Application for variation/cancellation Individual -3000/-
of the terms of Registration Educational –5000/-
Commercial–7000/- PV 10
Notice of opposition to application Rs 1500/- PV
11
for variation/cancellation of terms of
registration
Fees to be paid
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

Registration
• 18 years for trees and vines, from the date of Registration.
• Certificate of registration will be reviewed and renewed
after six years for annual crops and nine years for
trees/vines on payment of prescribed fees;
• For extant varieties, 15 years from date of notification of
that variety by the Central Government under section 5 of
the Seeds Act, 1966 and in other cases, 15years from the
date of registration of the variety. Sec24(6)
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

Benefit Sharing
• Section 26 deals with the Benefit Sharing.
• The claims under benefit sharing can be
submitted by Citizens of India or Firms /
NGOs formed or established in India.
• Such claims after due examination and after
receiving counter-claims will be disposed by
the Authority.
• The amount thus determined shall be
deposited by the breeder in the manner
referred to in clause(a) of sub-section(1) of
section 45 in the National Gene Fund.
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

• Sec 26(1) –upon grant of Registration certificate, authority
will advertise and invite claims of benefit sharing to the
variety
• Sec 26(2)-any person/community (Govt/Non Govt) can
claim and Authority shall inform breeder to submit
opposition under Sec 26(3)
• Authority will hear the parties Sec 26(4) and decide
amount of benefit sharing, if any, under 26(5) considering
the extent/nature of use of genetic material in
development of the variety and commercial utility and
demand in the market of the variety
• Benefit sharing to be deposited in the National Gene Fund
[Sec 26(6)]
• Amount of benefit sharing is recoverable as an arrear of
land revenue by DM within jurisdiction of local limits [Sec
26(7)]
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

What about Breeders?
• Breeder shall be required to deposit seeds or
propagating material including parental line seeds of
registered varieties in the National Gene Bank
(Sec27).
• A certificate of registration for a variety issued under
this Act shall confer an exclusive right on the breeder
or his success or, his agent or licensee, to produce,
sell, market, distribute, import or export the variety
[Sec28(1)]
• Breeders can appoint agent/ licensee and can opt for
civil remedy in case of infringement of his rights.
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

Researcher’s Rights (Sec 30)Section 30 states that:
• Nothing contained in this Act shall prevent-
✓ (a)The use of any variety registered under this Act by
any person using such variety for conducting
experiment or research
✓ (b)The use of a variety as an initial source of variety
for the purpose of creating of other varieties:
• Provided that the authorization of the breeder of
a registered variety is required where the
repeated use of such variety as a parental line is
necessary for commercial production of such
other newly developed variety.
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

Farmers’ Rights (Sec 39)
• A new variety bred/developed by farmer will be
registered in like manner as a breeder of a variety.
• Farmer engaged in conservation of genetic
resources of land races and wild relatives of
economic plants, improvement through selection
and preservation shall be entitled for recognition
and reward from Gene Fund provided that the
said material has been used as donors of genes in
varieties registrable under this Act.
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

• Farmers can save, use, sow, re-sow, exchange,
share or sell farm produce including seed of a
protected variety but can not sell branded
seed of a variety protected under this Act.
• Breeder shall disclose expected performance
of the protected variety under given
conditions and farmers can claim
compensation if variety fails.
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

Farmers’ variety
• Sec 2(l) states:
– Farmers’ variety means a variety which has
been traditionally cultivated and evolved by
farmers in their fields; or
• is a wild relative or land race of a
variety about which the farmers
possess the common knowledge
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

Crop Species for Registration
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

Species notified:
• Bread wheatRice
• Pearl milletSorghum
• MaizePigeon pea
• ChickpeaGreen gram
• Black gram Kidney bean/ French bean
• LentilField pea / Garden pea
• Cotton (4 species viz. Gossypiumhirsutum, G.
barbadense, G. arboretumand G. herbaceumL.)
• Jute (2 species viz. Corchorusolitoriusand C.
capsularis)
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

• Sugar caneGinger
• Turmeric Black pepper
• Small CardamomSunflower
• Safflower Castor
• SesameLinseed
• GroundnutSoybean
• Indian Mustard [Sarso(BrassicajunceaL.
Czern& Coss) and Karan Rai(B. carinataA.
Braun)]
• Rapeseed [Toria(B. rapaL.) and
GobhiSarson(B. napusL.)
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

Crops under notification:
• PotatoTomato
• BrinjalGarlic
• OnionOkra
• CabbageCauliflower
• RoseMango
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

New crops for which DUS Testing Guideline
Development is under process:
• Coconut Cashew
• Plantago (Isabgol) Coriander
• Rubber Tea
• Coffee Eucalyptus
• Casurina (02 species) Neem
• Jatropha Karanj
• Walnut Almond
• Apple Pear
• Cherry Apricot
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

• Bamboo (2 species) Sweet potato
• Cassava Orchids (3 genera)
• Mentha Brahmi
• Periwinkle Ashwagandha
• Rosa damascena Chrysanthemum
• Cucurbits (8 species) Citrus (3 species)
• Papaya Guava
• Banana Litchi
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

Thanks
Division
of
Fruits
and
Horticultural
Technology
ICAR-
Indian
Agricultural
Research
Institute,
New
Delhi
-
110012

Fruit variety registration and variety release procedures

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