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Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells

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D145: Genomics and Proteomics

Technologie
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Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells Michael Boutros, Amy A. Kiger, Susan Armknecht, Kim Kerr, Marc Hild, Britta Koch, Stefan A. Haas, Heidelberg Fly Array Consortium, Renato Paro, Norbert Perrimon (2004) Presenters: Lian Lim Luigi Leung
History of RNAi ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
RNAi and the Nobel Prize ,[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],Technicalities
Purpose of Study ,[object Object],[object Object],[object Object],[object Object]
Methods ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Methods/Results - Observe cells ,[object Object],[object Object],[object Object],[object Object]
Methods/Results - Observe cells ,[object Object],[object Object],[object Object]
Methods/Results - Observe cells ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Z-score Transformation ,[object Object],[object Object],[object Object],Cheadle, C., Vawter, M., Freed, W., Becker, K. “Analysis of Microarray Data Using Z Score Transformation.” J Mol Diagn 5.2 (2003 May): 73-81
Methods/Results - Observe cells ,[object Object],[object Object],[object Object]
Methods/Results - Observe cells ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Methods/Results - Observe cells ,[object Object],[object Object]
Methods/Results - Observe cells (z-score >3)
Methods/Results - Observe cells ,[object Object],[object Object]
Methods/Results - Further Analysis ,[object Object],[object Object],[object Object],[object Object],[object Object]
Method - Flow Cytometry
Methods/Results - Further Analysis ,[object Object]
Method - TUNEL ,[object Object]
Methods/Results - Further Analysis ,[object Object]
Methods - Rescue ,[object Object]
Methods/Results - Further Analysis ,[object Object]
Recap ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Importance of the Study ,[object Object],[object Object],[object Object]
Importance of RNAi ,[object Object],[object Object],[object Object],[object Object]
Further Reading RNAi – A powerful tool to unravel hepatitis C virus–host interactions within the infectious life cycle Joachim Lupberger, Laurent Brino, Thomas F. Baumert Focusing on RISC assembly in mammalian cells Junmei Hong, Na Wei, Alistair Chalk, Jue Wang, Yutong Song, Fan Yi, Ren-Ping Qiao, Erik L.L. Sonnhammer, Claes Wahlestedt, Zicai Liang, Quan Du Analysis of Microarray Data Using Z Score Transformation Cheadle, C., Vawter, M., Freed, W., Becker, K.
More on RNAi ,[object Object],[object Object],[object Object],[object Object]
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Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells

  • 1. Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells Michael Boutros, Amy A. Kiger, Susan Armknecht, Kim Kerr, Marc Hild, Britta Koch, Stefan A. Haas, Heidelberg Fly Array Consortium, Renato Paro, Norbert Perrimon (2004) Presenters: Lian Lim Luigi Leung
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  • 14. Methods/Results - Observe cells (z-score >3)
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  • 17. Method - Flow Cytometry
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  • 26. Further Reading RNAi – A powerful tool to unravel hepatitis C virus–host interactions within the infectious life cycle Joachim Lupberger, Laurent Brino, Thomas F. Baumert Focusing on RISC assembly in mammalian cells Junmei Hong, Na Wei, Alistair Chalk, Jue Wang, Yutong Song, Fan Yi, Ren-Ping Qiao, Erik L.L. Sonnhammer, Claes Wahlestedt, Zicai Liang, Quan Du Analysis of Microarray Data Using Z Score Transformation Cheadle, C., Vawter, M., Freed, W., Becker, K.
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