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4. hemostasis; hemodynamic disorders
1. Hemodynamic Disorders
Hemodynamic Disorders
Edema
Edema
Hyperemia and Congestion
Hyperemia and Congestion
Hemorrhage
Hemorrhage
Hemostasis and Thrombosis
Hemostasis and Thrombosis
Embolism
Embolism
Infarction
Infarction
Shock
Shock
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Dr. Krishna Tadepalli, MD, www.mletips.com
2. Feature
Hemostasis
Thrombosis
What is it?
Blood clot
Blood clot
Where is it?
Site of vascular
injury
In an uninjured
vessel
Why is it?
Trauma
(common)
Atherosclerosis
What happens
later?
Stops blood loss Produce
(deserved effect) complications
(Emboli)
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Dr. Krishna Tadepalli, MD, www.mletips.com
3. Hemostasis
Hemostasis
Events following vascular injury
Events following vascular injury
1. transient vasoconstriction ( by neurogenic
1. transient vasoconstriction ( by neurogenic
mechanisms, Endothelin – vasoconstrictor of
mechanisms, Endothelin – vasoconstrictor of
Endothelial cells), reduce blood loss
Endothelial cells), reduce blood loss
2. Primary Hemostasis formation of Primary
2. Primary Hemostasis formation of Primary
haemostatic plug (( by platelet adhesion, activation
haemostatic plug by platelet adhesion, activation
& Aggregation),
& Aggregation),
3. Secondary Hemostasis Platelets + Fibrin
3. Secondary Hemostasis Platelets + Fibrin
(( from coagulation pathways- either intrinsic or
from coagulation pathways- either intrinsic or
extrinsic), forms secondary haemostatic plug
extrinsic), forms secondary haemostatic plug
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Dr. Krishna Tadepalli, MD, www.mletips.com
4. Hemostasis
Hemostasis
Role of Endothelium
Role of Endothelium
1. Produce Pro-thrombotic (( Plasminogen
1. Produce Pro-thrombotic Plasminogen
activator inhibitors – PAI, Tissue factor, vWF)
activator inhibitors – PAI, Tissue factor, vWF)
and Anti-thrombotic factors (( Vasodilators –
and Anti-thrombotic factors Vasodilators –
PGI2, NO; tPA, Heparin like molecules &
PGI2, NO; tPA, Heparin like molecules &
Thombomodulin)
Thombomodulin)
2. Balance of these factors determine the end result
2. Balance of these factors determine the end result
(( clot or dissolution of clot)
clot or dissolution of clot)
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Dr. Krishna Tadepalli, MD, www.mletips.com
6. Coagulation pathway
Coagulation pathway
Role of Platelets
Role of Platelets
Granules α granules( contain clotting factors &
Granules α granules( contain clotting factors &
Growth factors), Dense or δ granules (( ADP, Ca++++,
Growth factors), Dense or δ granules ADP, Ca ,
Neuropeptides, Epinephrine)
Neuropeptides, Epinephrine)
Response to injury bind to sub-endothelial
Response to injury bind to sub-endothelial
ECM (( vWF + Gp -Ib) Adhesion
ECM vWF + Gp -Ib) Adhesion
Expose Phospholipid complexes (helps in intrinsic
Expose Phospholipid complexes (helps in intrinsic
clotting pathway) & release ADP& Ca++++
clotting pathway) & release ADP& Ca
Aggregate with other platelets (( with fibrin by Gp
Aggregate with other platelets with fibrin by Gp
IIb-IIIa on platelet surfaces
IIb-IIIa on platelet surfaces
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Dr. Krishna Tadepalli, MD, www.mletips.com
8. Aspirin in patients at risk for coronary thrombosis8
It irreversibly acetylates and inactivates cyclooxygenase-1 due to
its ability to block TxA2 synthesis EQ
Dr. Krishna Tadepalli, MD, www.mletips.com
11. Coagulation pathway
Coagulation pathway
Most important step in this pathway
Most important step in this pathway
(either intrinsic or extrinsic) formation of
(either intrinsic or extrinsic) formation of
Thrombin
Thrombin
Thrombin produces it effects through
Thrombin produces it effects through
PARS( Protease activated receptors – 7 GPCR
PARS( Protease activated receptors – 7 GPCR
family)
family)
Major effects of Thrombin
Major effects of Thrombin
Fibrin formation from Fibrinogen
Fibrin formation from Fibrinogen
On Platelets TxA2
On Platelets TxA2
On Endothelial cells NO, tPA, PGI2
On Endothelial cells NO, tPA, PGI2
act also on Lymphocytes, Neutrophils, Monocytes
act also on Lymphocytes, Neutrophils, Monocytes
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Dr. Krishna Tadepalli, MD, www.mletips.com
13. Anti -- Coagulants
Anti Coagulants
Natural ones
Natural ones
1. Antithrombins( AT-III) inactivate Thrombin;
1. Antithrombins( AT-III) inactivate Thrombin;
Factors IX to XII; bind to heparin like molecule on
Factors IX to XII; bind to heparin like molecule on
endothelium
endothelium
2. Proteins – C & S produced by liver; need Vit. K for
2. Proteins – C & S produced by liver; need Vit. K for
activation; inactivate Factors V, VIII
activation; inactivate Factors V, VIII
Tissue Factor Pathway Inhibitors (TFPI) inhibit
Tissue Factor Pathway Inhibitors (TFPI) inhibit
factors VII, X
factors VII, X
Clinical significance
Clinical significance
Heparin act through AT-III; Monitor PTT (Intrinsic pathway), Antidote is
Heparin act through AT-III; Monitor PTT (Intrinsic pathway), Antidote is
Protamine, can produce HIT (heparin induced thrombocytopenia); can be
Protamine, can produce HIT (heparin induced thrombocytopenia); can be
given in pregnancy (as it can’t cross placenta); need frequent injections
given in pregnancy (as it can’t cross placenta); need frequent injections
Warfarin act through inhibition of Vit K mediated carboxylation of
Warfarin act through inhibition of Vit K mediated carboxylation of
clotting factors (II,VII, IX, X); Extrinsic pathway Monitor PT; Antidote is
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clotting factors (II,VII, IX, X); Extrinsic pathway Monitor PT; Antidote is
Vit.K contraindicated in pregnancy (teratogenic)
Vit.K1 1;;contraindicated in pregnancy (teratogenic)
Dr. Krishna Tadepalli, MD, www.mletips.com
14. Fibrinolytic pathway
Fibrinolytic pathway
Most important step in this pathway
Most important step in this pathway
formation of Plasmin from Plasminogen
formation of Plasmin from Plasminogen
Tissue Plasminogen activator (tPA) is important in this
Tissue Plasminogen activator (tPA) is important in this
conversion
conversion
Plasmin breaks down excess Fibrin Fibrin split
Plasmin breaks down excess Fibrin Fibrin split
products or Fibrin degradation products (FSP or FDP)
products or Fibrin degradation products (FSP or FDP)
Most important component of FDP D-dimer
Most important component of FDP D-dimer
Clinical significance
Clinical significance
D-dimer positivity (≥200 ng) patient had clot and it is lysed by
D-dimer positivity (≥200 ng) patient had clot and it is lysed by
Plasmin
Plasmin
Conditions where D-dimer is positive DIC, DVT, PE
Conditions where D-dimer is positive DIC, DVT, PE
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Dr. Krishna Tadepalli, MD, www.mletips.com