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Plant tissue culture

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Plant tissue culture

  1. 1. PLANT TISSUE CULTURE Presented By Kamlesh Patade 1
  2. 2. CONTENTS 1. Introduction 2. History 3. Principle 4. Types of PTC 5. Applications 2
  3. 3. INTRODUCTION Plant tissue culture (PTC) is a form of asexual propagation of plants under laboratory conditions. Plant tissue culture is a technique with which plant cells, tissues or organs are grown on artificial nutrient medium, either static or liquid, under aseptic and controlled conditions. 3
  4. 4. HISTORY 1902 The idea of the totipotency of plant cell was given by Haberlandt 1937 White first time established successful root culture of tomato 1941 Vanoverbeek used coconut milk for growth and development of young Datura embryos 1957 Skoog and Miller demonstrated the role of auxin and cytokinin on root and shoot formation in tobacco – tissue 1962 Murashige and Skoog introduced the medium for tobacco culture 1987 Isolation of Bt. gene form bacterium Bacillus thuringiensis 4
  5. 5. PRINCIPLE PTC depends upon 1. Totipotency :- It is the ability of plant cells to regenerate into a whole plant 2. Plasticity :- It is the ability of plants to alter their metabolism, growth and development to best suit their environment 5
  6. 6. TYPES OF PTC Plant tissue culture Embryo culture Seed culture Meristem culture Cell culture Protoplast culture Callus culture Pollen culture Organ culture 6
  7. 7. 7
  8. 8. 8 Protoplast Fusion
  9. 9. Application of PTC Meristem culture 1. Production of virus free germplasm. 2. Mass production of desirable genotypes. 9
  10. 10. Anther culture/Pollen culture 1. Production of haploid plant 2. Production of useful gametoclonal variation. 10
  11. 11. Other Applications 1. Micropropagation can also be used to conserve rare or endangered plant species 2. Micropropagation using meristem and shoot culture to produce large numbers of identical individuals. 3. Large-scale growth of plant cells in liquid culture in bioreactors for production of valuable compounds 4. To cross distantly related species by protoplast fusion and regeneration of the novel hybrid 5. Production of identical sterile hybrid species can be obtained. 11
  12. 12. REFERENCES 1. Biotechnology by B. D. Singh 2. Biotechnology by U. Satyanarayana 3. agritech.tnau.ac.in 12
  13. 13. Thank You 13

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