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Activity 1 Light Microscopy
Bright Field Microscope ,[object Object]
Schematic Perspective
 
Advantage ,[object Object],[object Object],[object Object]
Specific Use ,[object Object],[object Object],[object Object]
Parts of Bright Field Microscope ,[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object]
Dark Field Microscope ,[object Object]
Schematic Perspective
Accessory Parts
Advantage ,[object Object],[object Object]
Specific Use ,[object Object],[object Object]
Phase Contrast Microscope ,[object Object]
Schematic Perspective
Accessory Parts
Advantage ,[object Object],[object Object]
Specific Use ,[object Object],[object Object]
Fluorescent Microscope ,[object Object]
Schematic Perspective
Accessory Parts
Advantage ,[object Object]
Specific Use ,[object Object],[object Object],[object Object]
Differential Interference Contrast Microscope General Perspective
Light Pathway in DIC Microscope
Inverted micoscope ,[object Object]
Light pathways of Inverted Microscope
Dissecting Microscope
Pathway of Light
Stereo Microscope ,[object Object]
Schematic Perspective
Sample illumination is via  transmitted white light . Simplicity of setup with only basic equipment required.  No sample preparation required, allowing viewing of live cells Bright Field Microscope Specific Use Advantage Types of Light  Microscope
Transparent specimens Cell parts are transparent Does not require staining living cells can be examined in their natural state Phase Contrast Microscope Live and biological samples Study of crystals and crystal defects Quality of image is impressive Raised features Dark field Microscope
Living cells and internal components are contrasted against the background giving greater definition and detail of cell structure Detect structures and molecules within the cell Fluorescent Microscope
Used for dissection to get a better look at the larger specimen.  Gives true color with 20x and 40x power using 10x eyepieces and rotating turret of 2x and 4x objectives  Dissecting Microscope Allow the specimen to remain active for long periods.  Allow to observe a living cells or organisms at the bottom of a large container  Inverted Microscope Allows the specimen to be seen more clearly Do not mask or otherwise obstruct the objective and condenser apertures  Differential Interference Contrast Microscope
designed long working distance objectives & wide field eyepieces produce an extremely large, brilliant & bright images.  Light in weight, fixed achromatic objectives 2x or 3x Stereo Microscope
Formulas ,[object Object],[object Object],[object Object]
Wavelength of ROYGBIV
Numerical Aperture ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
2.14 - 2.20  Cubic Zirconia  2.41  Diamond  1.73-1.89  Garnet 1.62  Tourmaline 1.54  Salt 1.54  Quartz 1.43 Fluorite 1.36  Ethyl alcohol 1.33  Water 1.31  Ice 1.000293  Air  Refractive Index   Substance
Steps in Preparing the Specimen ,[object Object],[object Object],[object Object]
[object Object]
Problems Encountered and Corrective Measures ,[object Object],[object Object],[object Object]
Hypothetical Problems ,[object Object]
[object Object],[object Object],[object Object],[object Object]
References ,[object Object],[object Object],[object Object],[object Object],[object Object]

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tHE lIGHT mICROSCOPE

  • 1. Activity 1 Light Microscopy
  • 2.
  • 4.  
  • 5.
  • 6.
  • 7.
  • 8.
  • 9.
  • 10.
  • 13.
  • 14.
  • 15.
  • 18.
  • 19.
  • 20.
  • 23.
  • 24.
  • 25. Differential Interference Contrast Microscope General Perspective
  • 26. Light Pathway in DIC Microscope
  • 27.
  • 28. Light pathways of Inverted Microscope
  • 31.
  • 33. Sample illumination is via transmitted white light . Simplicity of setup with only basic equipment required. No sample preparation required, allowing viewing of live cells Bright Field Microscope Specific Use Advantage Types of Light Microscope
  • 34. Transparent specimens Cell parts are transparent Does not require staining living cells can be examined in their natural state Phase Contrast Microscope Live and biological samples Study of crystals and crystal defects Quality of image is impressive Raised features Dark field Microscope
  • 35. Living cells and internal components are contrasted against the background giving greater definition and detail of cell structure Detect structures and molecules within the cell Fluorescent Microscope
  • 36. Used for dissection to get a better look at the larger specimen. Gives true color with 20x and 40x power using 10x eyepieces and rotating turret of 2x and 4x objectives Dissecting Microscope Allow the specimen to remain active for long periods. Allow to observe a living cells or organisms at the bottom of a large container Inverted Microscope Allows the specimen to be seen more clearly Do not mask or otherwise obstruct the objective and condenser apertures Differential Interference Contrast Microscope
  • 37. designed long working distance objectives & wide field eyepieces produce an extremely large, brilliant & bright images. Light in weight, fixed achromatic objectives 2x or 3x Stereo Microscope
  • 38.
  • 40.
  • 41. 2.14 - 2.20 Cubic Zirconia 2.41 Diamond 1.73-1.89 Garnet 1.62 Tourmaline 1.54 Salt 1.54 Quartz 1.43 Fluorite 1.36 Ethyl alcohol 1.33 Water 1.31 Ice 1.000293 Air Refractive Index Substance
  • 42.
  • 43.
  • 44.
  • 45.
  • 46.
  • 47.