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General Microbiology 
Difference Between Prokaryotic 
And Eukaryotic Cell 
By 
Jamseel.M
Cell 
The cell Latin cella, meaning "small room. 
Basic structural, functional and biological unit 
of all known living organisms. 
Cells are the smallest unit of life that can 
replicate independently, 
"building blocks of life“ 
There are two types of cells, eukaryotes, 
which contain a nucleus, and prokaryotes, 
which do not.
PROKARYOTIC 
CELLS
Functions of Cells 
A boundary that keeps the cellular contents 
separate from the external environment but 
allows for the transfer of some substances 
into and out of the cell. 
Replication of DNA 
Synthesis of cellular components 
The ability to obtain energy through 
metabolic processes
Appendages are basically involved in 
movement or adhesion 
Flagella (singular flagellum) are 
cellular appendages that consist of 
three parts: 
1. A filament that rotates for movement 
2. A hook where the filament attaches 
3. A basal body that anchors the hook 
to the cell 
The arrangement of the hook/basal 
body articulation allows the hook with 
its filament to rotate 360o
Arrangements of 
Flagella
Axial filaments 
Modified flagella 
that occur in 
spirochetes
Non-locomotor 
appendages 
Pili are longer 
and sparser 
than fimbriae. 
fimbriae are 
involved in 
adhesion and 
pili (found only 
in Gram - 
bacteria) are 
involved in 
conjugation ( a 
“mating” 
process).
Cell envelopes differ between taxa 
but they basically consist of three 
layers: 
 The capsule or slime layer (outermost layer) 
differs greatly in thickness, organization and 
chemical composition depending on the 
bacterial species. 
 Beneath the outer layer lies the cell wall. 
 The cell membrane is a thin flexible sheet 
that surrounds the contents of the bacterial 
cell. Its functions include: transport, energy 
extraction, nutrient processing, and synthesis
The Gram Stain
The protoplasm or cytoplasm 
o Dense gelatinous solution within the cell 
membrane 
o Primary site for the cell’s biochemical and 
synthetic processes.
Nuclear region 
chromatin body or 
the bacterial 
chromosome 
Nucleoid or nuclear 
region of the cell 
that is associated 
with the chromatin 
body
Plasmids 
Plasmids are tiny 
circular extra 
chromosomal strands of 
DNA 
 Ribosomes are small 
structures consisting of 
RNA and proteins that 
are involved in protein 
synthesis
Inclusions or granules 
Inclusions or granules 
are areas where 
nutrients are 
concentrated 
Endospores are 
dormant structures 
produced by some 
species of Bacillus and 
Clostridium.
Shapes and 
arrangements of 
bacteria
FUNGI 
 Fungi are eukaryotic protista; differ from 
bacteria and other prokaryotes. 
1. Cell walls containing chitin (rigidity & 
support) ,other polysaccharides 
2. Cytoplasmic membrane contains ergosterols 
3. Possess true nuclei with nuclear membrane 
& paired chromosomes. 
4. Divide asexually, sexually or by both 
5. Unicellular or multicellular
• Simplest fungus :- Unicellular 
budding yeast 
• Hypha :- Elongation of apical cell 
produces a tubular, thread like 
structure called hypha 
• Mycelium :- Tangled mass of 
hyphae is called mycelium. Fungi 
producing mycelia are called 
molds or filamentous fungi. 
• Hyphae may be septate or non-septate
CLASSIFICATION 
• Depending on cell morphology 
1. Yeasts 
2. Yeast like fungi 
3. Molds 
4. Dimorphic fungi
Eukaryotic Cell
CELL MEMBRANE 
StructureComponents 
Arrangement 
Functions 
Barrier 
Transport (know diffusion, osmosis, 
facilitated diffusion and active 
transport) 
Recognition (e.g., self vs. non-self) 
Reception (for protein hormones)
Nucleus 
Structure and 
Function 
– membrane similar 
to cell membrane 
(similar function) 
– Nucleolus 
(formation of 
ribosomes) 
– Chromosomes 
(gene expression) 
– Nucleoplasm 
(matrix)
Ribosomes 
 Structure 
– rRNA 
– Proteins 
Function 
– Site of protein 
formation 
(translation) 
Found in both 
prokaryotes and 
eukaryotes (different 
structurally)
Endoplasmic 
Reticulum 
 Structure 
membranous system 
of tunnels and sacs 
– Rough – with 
ribosomes on 
surface 
– Smooth- no 
ribosomes on 
surface 
 Function 
– Rough – protein 
synthesis 
– Smooth- lipid 
synthesis
Golgi Apparatus 
Structure also membranous, kind of 
like a stack of pancakes 
Function processing of lipids and 
proteins
LYSOSOMES 
Structure 
Membrane bound sac containing 
hydrolytic enzymes 
 Function 
Digestion
Mitochondria 
Structure – cigar-shaped, 
double 
membrane-bound 
organelle 
Function – Energy 
transfer by ATP 
synthesis
Chloroplast 
Structure 
Also cigar or spindle 
shaped, double 
membrane-bound, green 
Function 
photosynthesis
Other structure 
• Cell walls, not in animal cells 
• Vacuoles 
• Cytoskeleton 
• Cytoplasm
Differences Between 
Prokaryotic and Eukaryotic 
cells
No 
Features 
1. Occurrence Prokaryotic 
cells are the 
characteristic 
of bacteria 
and blue 
green algae 
These are cells 
are found in all, 
animals and 
plants, except 
blue green 
algae and 
bacteria.
2 
. 
Size 
Mostly 
1-10 μm 
Mostly 
10-100 μm 
3 
. 
Multicellular 
forms 
Rare 
Common, with 
extensive 
tissue 
formation
4. Cell wall 
Present in most but not 
in all cells. In Bacteria, 
cell wall is made up 
murein,polysaccharides, 
lipid and proteins. 
The animal 
cells lack 
cell wall, 
but plants 
cell wall is 
made up of 
cellulose 
and 
chitinous 
cell wall is 
present in 
fungi.
5. 
Plasma 
membrane Present 
PPrreesseenntt 
Present 
6 
Nucleus
7. 
Nuclear 
Membranes 
Absent 
Present 
8. Chromatin with 
histone 
Absent Present 
9. Number of 
chromosomes 
Each cell 
Has only 
one 
chromosome 
Number of 
chromosomes 
per cell 
depends 
upon the 
type of 
organism.
10 Chromosome 
The Chromose 
is circular ring 
lacking a 
centromere. 
Each chromosome 
is linear having 
a centromere 
11 
. 
Genetic material Circular or 
linear, double 
stranded 
DNA: only 
exons are 
present 
Linear double 
stranded DNA: 
genes frequently 
interrupted by 
intron sequences, 
especially in 
higher 
eukaryotes 
(called as split 
genes).
12 
Nucleoli and 
Mitotic apparatus 
Absent 
Present 
13. Nucleolus Absent Present (for 
the synthesis 
and 
organization 
of ribosomes)
14. Plasmid Commonly 
present 
Rare 
15 Mesosomes Mesosomes 
perform the 
function of Golgi 
bodies and 
mitochondria, 
and also help in 
the separation 
of chromosomes 
during cell 
division. 
Absent
Mitochondria Absent Present 
Endoplasmic 
Reticulum 
Vacuoles 
Absent 
Absent 
Present 
Present 
16. Cell organelles
Lysosomes Absent Present 
Chloroplast Absent Present 
Centrioles Absent Present 
Ribosomes Only 70S type of 
ribosomes are 
present which lie 
free in cytoplasm, 
or are engaged in 
protein synthesis. 
The cytoplasm 
has 80S type of 
ribosomes; and 
plastid and 
mitochondria 
have 70 S
Microtubules Absent Present 
Flagellae 
Simple structure 
Composed of the 
protein Flagellin. 
Complex 
9+2 
Structure 
of tubulin 
and other 
protein.
Respiration 
Many strict 
anaerobes 
All aerobic, 
but some 
facultative 
BAyn aerobes 
secondary 
.modifications 
17 
18 
Photosynthetic 
Enzymes 
Bound to 
plasma 
membrane as 
composite 
chromatophore 
Enzymes 
packed 
in plastids 
bound 
by 
membrane
19 
Metabolic 
Patterns 
Great Variations 
All share cytochrome 
electron transport 
chains, Krebs cycle 
oxidation, glycolysis. 
20 
Sexual 
System 
Rare: If present one 
way (and usually 
forming partial 
diploids or 
merozygotes) 
Both sexes 
involved in sexual 
participation and 
entire genomes 
transferred
21 
Cyclosis 
There are no 
streaming 
movements 
of cytoplasm 
Cytoplasm 
shows 
streaming 
movements 
22 
Protein 
Synthesis 
Transcription 
and translation 
take place in 
cytoplasm. 
Transcription 
occurs in 
nucleus 
and translation 
takes place in 
cytoplasm. 
23 
Duration 
of cell 
cycle 
Cell cycle is short, 
takes 20-60 
minutes 
to complete. 
Cell cycle is 
long, takes 
12-24 hours to 
complete.
CONCLUSION 
 Cell are basic unit of organization or structure of 
all living matter 
 There are two types of cell,that are prokaryotic 
and eukaryotic cell 
 There are are so many difference in between 
them include occurance,size,cell wall, nucleus 
,nuclear membrane, cell organelles respiration, 
sexual system ,protein synthesis.
REFERENCE 
Prescott LM Harley JP and Klein DA-Microbiology 
John Webster-Introduction to fungi 
Voet and voet 
Tortora-Microbiology an introduction 
Pelczar Jr.MJ Chan,Ecs and Kreig- 
Microbiology 
Lehninger’s principle of biochemistry
DIFFERENCE BETWEEN PROKARYOTIC AND EUKARYOTIC

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DIFFERENCE BETWEEN PROKARYOTIC AND EUKARYOTIC

  • 1. General Microbiology Difference Between Prokaryotic And Eukaryotic Cell By Jamseel.M
  • 2. Cell The cell Latin cella, meaning "small room. Basic structural, functional and biological unit of all known living organisms. Cells are the smallest unit of life that can replicate independently, "building blocks of life“ There are two types of cells, eukaryotes, which contain a nucleus, and prokaryotes, which do not.
  • 4.
  • 5. Functions of Cells A boundary that keeps the cellular contents separate from the external environment but allows for the transfer of some substances into and out of the cell. Replication of DNA Synthesis of cellular components The ability to obtain energy through metabolic processes
  • 6. Appendages are basically involved in movement or adhesion Flagella (singular flagellum) are cellular appendages that consist of three parts: 1. A filament that rotates for movement 2. A hook where the filament attaches 3. A basal body that anchors the hook to the cell The arrangement of the hook/basal body articulation allows the hook with its filament to rotate 360o
  • 7.
  • 9. Axial filaments Modified flagella that occur in spirochetes
  • 10. Non-locomotor appendages Pili are longer and sparser than fimbriae. fimbriae are involved in adhesion and pili (found only in Gram - bacteria) are involved in conjugation ( a “mating” process).
  • 11. Cell envelopes differ between taxa but they basically consist of three layers:  The capsule or slime layer (outermost layer) differs greatly in thickness, organization and chemical composition depending on the bacterial species.  Beneath the outer layer lies the cell wall.  The cell membrane is a thin flexible sheet that surrounds the contents of the bacterial cell. Its functions include: transport, energy extraction, nutrient processing, and synthesis
  • 12.
  • 14. The protoplasm or cytoplasm o Dense gelatinous solution within the cell membrane o Primary site for the cell’s biochemical and synthetic processes.
  • 15. Nuclear region chromatin body or the bacterial chromosome Nucleoid or nuclear region of the cell that is associated with the chromatin body
  • 16. Plasmids Plasmids are tiny circular extra chromosomal strands of DNA  Ribosomes are small structures consisting of RNA and proteins that are involved in protein synthesis
  • 17. Inclusions or granules Inclusions or granules are areas where nutrients are concentrated Endospores are dormant structures produced by some species of Bacillus and Clostridium.
  • 18. Shapes and arrangements of bacteria
  • 19. FUNGI  Fungi are eukaryotic protista; differ from bacteria and other prokaryotes. 1. Cell walls containing chitin (rigidity & support) ,other polysaccharides 2. Cytoplasmic membrane contains ergosterols 3. Possess true nuclei with nuclear membrane & paired chromosomes. 4. Divide asexually, sexually or by both 5. Unicellular or multicellular
  • 20. • Simplest fungus :- Unicellular budding yeast • Hypha :- Elongation of apical cell produces a tubular, thread like structure called hypha • Mycelium :- Tangled mass of hyphae is called mycelium. Fungi producing mycelia are called molds or filamentous fungi. • Hyphae may be septate or non-septate
  • 21. CLASSIFICATION • Depending on cell morphology 1. Yeasts 2. Yeast like fungi 3. Molds 4. Dimorphic fungi
  • 23. CELL MEMBRANE StructureComponents Arrangement Functions Barrier Transport (know diffusion, osmosis, facilitated diffusion and active transport) Recognition (e.g., self vs. non-self) Reception (for protein hormones)
  • 24.
  • 25. Nucleus Structure and Function – membrane similar to cell membrane (similar function) – Nucleolus (formation of ribosomes) – Chromosomes (gene expression) – Nucleoplasm (matrix)
  • 26. Ribosomes  Structure – rRNA – Proteins Function – Site of protein formation (translation) Found in both prokaryotes and eukaryotes (different structurally)
  • 27. Endoplasmic Reticulum  Structure membranous system of tunnels and sacs – Rough – with ribosomes on surface – Smooth- no ribosomes on surface  Function – Rough – protein synthesis – Smooth- lipid synthesis
  • 28. Golgi Apparatus Structure also membranous, kind of like a stack of pancakes Function processing of lipids and proteins
  • 29. LYSOSOMES Structure Membrane bound sac containing hydrolytic enzymes  Function Digestion
  • 30. Mitochondria Structure – cigar-shaped, double membrane-bound organelle Function – Energy transfer by ATP synthesis
  • 31. Chloroplast Structure Also cigar or spindle shaped, double membrane-bound, green Function photosynthesis
  • 32. Other structure • Cell walls, not in animal cells • Vacuoles • Cytoskeleton • Cytoplasm
  • 33. Differences Between Prokaryotic and Eukaryotic cells
  • 34. No Features 1. Occurrence Prokaryotic cells are the characteristic of bacteria and blue green algae These are cells are found in all, animals and plants, except blue green algae and bacteria.
  • 35. 2 . Size Mostly 1-10 μm Mostly 10-100 μm 3 . Multicellular forms Rare Common, with extensive tissue formation
  • 36. 4. Cell wall Present in most but not in all cells. In Bacteria, cell wall is made up murein,polysaccharides, lipid and proteins. The animal cells lack cell wall, but plants cell wall is made up of cellulose and chitinous cell wall is present in fungi.
  • 37. 5. Plasma membrane Present PPrreesseenntt Present 6 Nucleus
  • 38. 7. Nuclear Membranes Absent Present 8. Chromatin with histone Absent Present 9. Number of chromosomes Each cell Has only one chromosome Number of chromosomes per cell depends upon the type of organism.
  • 39. 10 Chromosome The Chromose is circular ring lacking a centromere. Each chromosome is linear having a centromere 11 . Genetic material Circular or linear, double stranded DNA: only exons are present Linear double stranded DNA: genes frequently interrupted by intron sequences, especially in higher eukaryotes (called as split genes).
  • 40. 12 Nucleoli and Mitotic apparatus Absent Present 13. Nucleolus Absent Present (for the synthesis and organization of ribosomes)
  • 41. 14. Plasmid Commonly present Rare 15 Mesosomes Mesosomes perform the function of Golgi bodies and mitochondria, and also help in the separation of chromosomes during cell division. Absent
  • 42. Mitochondria Absent Present Endoplasmic Reticulum Vacuoles Absent Absent Present Present 16. Cell organelles
  • 43. Lysosomes Absent Present Chloroplast Absent Present Centrioles Absent Present Ribosomes Only 70S type of ribosomes are present which lie free in cytoplasm, or are engaged in protein synthesis. The cytoplasm has 80S type of ribosomes; and plastid and mitochondria have 70 S
  • 44. Microtubules Absent Present Flagellae Simple structure Composed of the protein Flagellin. Complex 9+2 Structure of tubulin and other protein.
  • 45. Respiration Many strict anaerobes All aerobic, but some facultative BAyn aerobes secondary .modifications 17 18 Photosynthetic Enzymes Bound to plasma membrane as composite chromatophore Enzymes packed in plastids bound by membrane
  • 46. 19 Metabolic Patterns Great Variations All share cytochrome electron transport chains, Krebs cycle oxidation, glycolysis. 20 Sexual System Rare: If present one way (and usually forming partial diploids or merozygotes) Both sexes involved in sexual participation and entire genomes transferred
  • 47. 21 Cyclosis There are no streaming movements of cytoplasm Cytoplasm shows streaming movements 22 Protein Synthesis Transcription and translation take place in cytoplasm. Transcription occurs in nucleus and translation takes place in cytoplasm. 23 Duration of cell cycle Cell cycle is short, takes 20-60 minutes to complete. Cell cycle is long, takes 12-24 hours to complete.
  • 48. CONCLUSION  Cell are basic unit of organization or structure of all living matter  There are two types of cell,that are prokaryotic and eukaryotic cell  There are are so many difference in between them include occurance,size,cell wall, nucleus ,nuclear membrane, cell organelles respiration, sexual system ,protein synthesis.
  • 49. REFERENCE Prescott LM Harley JP and Klein DA-Microbiology John Webster-Introduction to fungi Voet and voet Tortora-Microbiology an introduction Pelczar Jr.MJ Chan,Ecs and Kreig- Microbiology Lehninger’s principle of biochemistry