SAB presentation to Jim narrated

1. Ecological and evolutionary functional genomics: finding & studying genes that matter Christopher W. Wheat

3. Rapid transcriptome characterization for a nonmodel organism using 454 pyrosequencing C. Vera and C. Wheat, Fescemyer, Frilander, Crawford, Hanski, Marden 2008

4. 48k contigs

5. First de novo transcriptome assembly using 454 pyrosequencing SNP’s

6. Annotation of 45,000 contigs + singletons Predicted genes: D. melanogaster = 13,379 B. mori = 18,510 Estimated coverage : 70% D. mel estimate 50% B. mori estimate Lower estimate 50% genes

7. Upper estimate of 70% = 13,142 genes

13. Mixed Model Analysis : popage & peak metabolic rate Popage fixed effects: dye popage bodymass popage*bodymass PMR fixed effect: dye PMR Random effects: slide array(slide) spot spot*array individual (FDR threshold = 5%) Using JMP Genomics (SAS)

15. Significantly different genes (FDR threshold = 5%) Peak Metabolic Rate Continuous Wheat et al., in prep. 623 33 188 656 Population Age 221 Categorical

18. Temperature treatments Last instar larvae Reared in lab Study gene expression Kvist et al., in prep. 20 o C 26 o C 35 o C Temperature maximum Cold Standard Hot Treatments Hot Standard Cold