1. Study of potential prophylactic effect by marennine
by utilisation of Haslea ostrearia Simonsen as
oyster’s feed
Presented by:
Fiddy S. PRASETIYA
Supervised by:
Jean-Luc MOUGET
Bruno COGNIE
Michèle MORANCAIS
Yann HARDIVILLIER
Romain GASTINEAU
Fiddy SEMBA PRASETIYA, 1st year PhD student Université du Maine
Study of potential prophylactic effect by marennine by utilisation of Haslea ostrearia Simonsen as oyster’s feed

2. • To determine the allelopathic effect of H. ostrearia and
external marennine on the growth of other species of
diatoms
Introduction Objective Methodology Results Conclusion
Fiddy SEMBA PRASETIYA
Study of potential prophylactic effect by marennine by utilisation of Haslea ostrearia Simonsen as oyster’s feed
2

3. Introduction Objective Methodology Results Conclusion
Fiddy SEMBA PRASETIYA
Study of potential prophylactic effect by marennine by utilisation of Haslea ostrearia Simonsen as oyster’s feed
3
Culture preparation:
- H. ostrearia
- T. suecica
- C. gracilis
- I. galbana (T. Iso)
- S. costatum
- P. lutheri
- C. calcitrans
Measurement of :
- Biovolume
- Growth rate
Preparation of allelopathy
experiment:
- H. ostrearia + T. suecica, etc
(based on biovolume
comparison), vol. max. 150 ml
Observation algal
growth and biovolume
under microscope,
Marennine in each
culture sampled (10
days)for HPLC analysis

4. Introduction Objective Methodology Results Conclusion
Fiddy SEMBA PRASETIYA
Study of potential prophylactic effect by marennine by utilisation of Haslea ostrearia Simonsen as oyster’s feed
4
Comparison of biovolume & growth rate of each diatoms species
0
100
200
300
400
500
600
700
0 4 6 8 10 12 14
Cellconcentration*x10⁴+(cell/mL)
Time (days)
Growth rate of control
H.ostrearia
T.suecica
C.gracilis
I.galbana
S.costatum
C.calcitrans
P.lutheri 0
10
20
30
40
50
60
70
80
0 4 6 8 10 12 14
Volume(µL)
Time (days)
Biovolume of control
H.ostrearia
T.suecica
C.gracilis
I.galbana
S.costatum
C.calcitrans
P.lutheri

5. Introduction Objective Methodology Results Conclusion
Fiddy SEMBA PRASETIYA
Study of potential prophylactic effect by marennine by utilisation of Haslea ostrearia Simonsen as oyster’s feed
5
0
20
40
60
80
100
120
140
0 4 6 8 10
Concentration(x104cell/ml)
0.00
10.00
20.00
30.00
40.00
50.00
60.00
70.00
80.00
0 4 6 8 10Volume(µL)
T. suecica (Control)
T. suecica (treatement)
H.ostrearia (Control)
H.ostrearia
(+T.suecica)
Comparison of allelopathic effect on growth rate & biovolume of T. Suecica & C. gracilis
0
20
40
60
80
100
120
0 4 6 8 10
Concentration(x10⁴Cell/mL)
Times (days)
0.000
10.000
20.000
30.000
40.000
50.000
60.000
0 4 6 8 10
Volume(µL)
Time (days)
C.gracilis (Control)
C.gracilis (treatement)
H.ostrearia (Control)
H.ostrearia
(+C.gracilis)

6. Introduction Objective Methodology Results Conclusion
Fiddy SEMBA PRASETIYA
Study of potential prophylactic effect by marennine by utilisation of Haslea ostrearia Simonsen as oyster’s feed
6
Comparison of allelopathic effect on growth rate & biovolume of T. iso (16°) & T. iso (21°)
0
100
200
300
400
500
600
0 4 6 8 10
Concentration(x104cell/ml)
0.000
10.000
20.000
30.000
40.000
50.000
0 4 6 8 10
Volume(µL)
T. iso (Control)
T. iso (treatement)
H.ostrearia (Control)
H.ostrearia (+T. iso)
0
100
200
300
400
500
600
0 4 6 8 10
Concentration(x104cell/ml)
Time (days)
0
10
20
30
40
50
60
0 4 6 8 10
Volume(µL)
Time (days)
T. iso 21 (Control)
T. iso 21 (treatement)
H.ostrearia (Control)
H.ostrearia (+ T. iso 21 )

7. Introduction Objective Methodology Results Conclusion
Fiddy SEMBA PRASETIYA
Study of potential prophylactic effect by marennine by utilisation of Haslea ostrearia Simonsen as oyster’s feed
7
Comparison of allelopathic effect on growth rate & biovolume of S. costatum & C. Calcitrans
0
20
40
60
80
100
120
0 4 6 8 10
Concentration(x104cell/ml)
0.00
10.00
20.00
30.00
40.00
50.00
0 4 6 8 10
Volume(µL)
S. costatum (Control)
S. costatum (treatement)
H.ostrearia (Control)
H.ostrearia (+S. costatum)
0
20
40
60
80
100
120
0 4 6 8 10
Concentration(x10⁴Cell/mL)
Times (days)
0.000
10.000
20.000
30.000
40.000
50.000
60.000
1 2 3 4 5
Volume(µL)
Time (days)
C.calcitrans (Control)
C.cancitrans (treatement)
H.ostrearia (Control)
H.ostrearia (+C.calcitrans)

8. Introduction Objective Methodology Results Conclusion
Fiddy SEMBA PRASETIYA
Study of potential prophylactic effect by marennine by utilisation of Haslea ostrearia Simonsen as oyster’s feed
8
Comparison of allelopathic effect on growth rate & biovolume of P. lutheri
0
20
40
60
80
100
120
0 4 6 8 10
Concentration(x104cell/ml)
Time (days)
0.00
10.00
20.00
30.00
40.00
50.00
0 4 6 8 10
Volume(µL)
Time (days)
P. Lutheri (Control)
P. Lutheri (treatement)
H.ostrearia (Control)
H.ostrearia (+P. Lutheri)