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Plagas que amenazan a las palmas en Puerto rico Jose Carlos V. Rodrigues [email_address] Casa Abierta UPR EEA CID Rio Piedras 2:10-2:50PM April 30’2010
Acknowledgment ,[object Object],[object Object]
The generalist Needed: a New generation of problem solvers John W. MCarthur Jeffrey Sachs The Chronicle  Jun/2009
The Big Fish Eat the Small Ones  by  Peter Brueghel the Elder  ( 1556 ).
Adult coconut palm showing “drought syndrome”  Humacao/PR Example 1. Raoiella indica  - red palm mite
Coconut palm
Coconut damage by  Raoiella
Introduction ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Background information ,[object Object],[object Object],[object Object],[object Object]
Example 1. Raoiella indica  - red palm mite
Feeding behavior  ----->>>> Stomatal conductance Example 1. Raoiella indica  - red palm mite
Stomatal conductance Mite - feeding behavior 21 days after infestation 1 mite/ cm2 Mite’s stylets Guard cells stomata mmol/cm2.s
Does the reduction on conductance associated with RPM feeding damage is affecting plant grown? 3 months  after treatment Example 1. Raoiella indica  - red palm mite
“ Coconut palm” Example 1. Raoiella indica  - red palm mite
Red palm mite damage - Luquillo and Dorado/PR Example 1. Raoiella indica  - red palm mite
Pruning
Coconut and banana
Damage in plantain by  Raoiella
Red palm mite damage in banana Loiza/PR Example 1. Raoiella indica  - red palm mite
Number of  Raoiella indica  mites on the second youngest expanded leaf of different  Musa  spp. hybrids. Data are averaged for ten replicates. Significance p<0.05. T3 = 3months post infestation
Status of  Raoiella  - Caribbean  2007 Florida (Nov) (Oct) 2009 Brazil  (Roraima) 2007 2008 (May)
October 2006 - First detection  in Puerto Rico and Culebra January 2008 (Source Aphis)
Photographer of bison hides; JR Douglass; 1874 Prey- predator relationships
Prey-predator relationships A -  Amblyseius largoensis  (Muma),  B - Mitochondrial DNA COI fragment, ( C )  Raoiella indica  DNA sequence __ 400pb A B C
Raoiella indica  vs.  Amblyseius largoensis Sampling Unit 10 leaflets/plant ETOH 80% FAO
Total number of arthropods collected 07/08 Average of 10 leaflets/plant Raoiella indica Amblyseius largoensis Thrips Others N 20 km
Credits: USDA/ARS
Chemical composition of mite’s drops NMR GC-MS
Example 2. Palms’ dieback
Example 2. Palms’ dieback
Example 2. Palms’ dieback
Example 2. Palms’ dieback
Example 2. Palms’ dieback Lethal Yellowing symptoms PCR and Nested PCR detection 1.4Kb
Example 2. Palms’ dieback
Real Palm
Example 2. Palms’ dieback
Example 2. Palms’ dieback Insects PCR and Nested PCR detection
Remarks ,[object Object],[object Object],[object Object],[object Object],[object Object]
New Quarantine  and Invasive Species Center for Excellence Jardin Botánico Sur, Río Piedras Agricultural Experiment Station Welcome / Bienvenidos Jose Carlos V. Rodrigues [email_address]
Birds at Real Palm
Acknowledgment ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
2011 APS-CD & ESA-SEB join meeting March 19-22  San Juan, PR

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Plagas que amenazan a las palmas en Puerto Rico – Dr. José C. Rodrigues

Hinweis der Redaktion

  1. Why working in Puerto Rico?
  2. 39027 Raoiella indica on Coconut Palm leaves from St. Lucia, West Indies. Dr. Ron Ochoa
  3. Because of the mite feeding habits - through the stomata, the stomatal conductance is quickly and drastically affected
  4. The mite was described originally from India and here is the status on Caribbean
  5. We extrated DNA from single potential predators and were able to recover Raoiella (DNA) from them.
  6. We established 10leaflets as sampling unit and notice a positive correlation between Amblyseius and Raoiella
  7. We sampled mites all around the islan and determine the temporal dynamics for each place