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Cell & Molecular
Biology
Enzyme
and Generally
Protein
Regulation
Chapters 3 & 4
Enzyme
Kinetics
Turnover Number
“...how rapidly
the substrate
can be processed.”
Figure 3-26a Essential Cell Biology (© Garland Science 2010)
Figure 3-26b Essential Cell Biology (© Garland Science 2010)
Figure 3-24 Essential Cell Biology (© Garland Science 2010)
Enzymes and substrates
encounter each other
through diffusion.
Figure 3-22 Essential Cell Biology (© Garland Science 2010)
Inhibitors
Estrogen receptor
with DNA and
estradiol bound



         http://www.pdb.org/pdb/static.do?
         p=education_discussion/
         molecule_of_the_month/pdb45_1.html
Tamoxifen
      blocks
      the action
      of estrogen



http://www.pdb.org/pdb/static.do?
p=education_discussion/
molecule_of_the_month/pdb45_1.html
Competitive
Inhibition
Figure 3-28a Essential Cell Biology (© Garland Science 2010)
Figure 3-28b Essential Cell Biology (© Garland Science 2010)
Trypsin
           h"p://cnx.org/content/m11456/latest/




          Benzamidine
Noncompetitive
Inhibition
Feedback
Inhibition
Figure 4-34 Essential Cell Biology (© Garland Science 2010)
Negative
Regulation
Figure 4-35 Essential Cell Biology (© Garland Science 2010)
Figure 4-36 Essential Cell Biology (© Garland Science 2010)
Allosteric
Regulation
Protein
Phosphorylation
The reversible addition
of a phosphate group
to a protein.
Figure 4-38a Essential Cell Biology (© Garland Science 2010)
Figure 4-38b Essential Cell Biology (© Garland Science 2010)
GTP Binding
Figure 4-39 Essential Cell Biology (© Garland Science 2010)
Elongation Factor
EF-Tu


tRNA



           http://www.rcsb.org/pdb/
           101/motm.do?momID=81
MCB: Enzymatic Pathways and Regulation

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