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annperry09

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DNA TECHNOLOGY DNA Profiling & Identification

Use of DNA ,[object Object],[object Object],[object Object]

Success of DNA in Courtroom ,[object Object],[object Object],[object Object],[object Object],[object Object]

Methods of DNA Analysis ,[object Object],[object Object],[object Object],[object Object],[object Object]

RFLP - restriction ,[object Object],[object Object],[object Object]

RFLP – gel electrophoresis ,[object Object],[object Object],[object Object]

RFLP – hybridization ,[object Object],[object Object],[object Object]

RFLP - analysis ,[object Object],[object Object]

RFLP – pros/cons ,[object Object],[object Object],[object Object],[object Object]

PCR Analysis ,[object Object],[object Object],[object Object],[object Object]

STR Analysis ,[object Object],[object Object]

mtDNA Analysis ,[object Object],[object Object]

Y-Chromosome Analysis ,[object Object],[object Object],[object Object],[object Object]

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What unique properties of the genome do forensic scientists use to generate a DNA footprint and this footprint enough to identify a specific (one) suspect? Solution only one tenth of a single percent of DNA differs from one person to another, forensic scientist use these variable regions to generate a DNA profile of an individual using samples from blood , hair ,etc. scientist find markers in a DNA sample by designing a small pieces of DNA(probes) that will bind to a complementary DNA sequence in the sample.A series of probes bound to a DNA sample creates a distinctive pattern for individual.scientist compare these DNA profile to determine whether the suspect\'s sample matches the evidence sample.if they don\'t match the person don\'t contribute to the crime scene.If it match it means the suspect may have contributes to the evidence sample. There are variable number of tendom repeats which are used to identify the suspects, which vary in each person. RFLP(restriction fragment length polymorphism ) is a technique used in this concern as it analyze variable length of DNA fragments that results from digesting a DNA sample with a special kind of enzyme. various other techniques are also there as- PCR,STAR analysis.

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Forensic science is defined as the application of scientific knowledge and experimentation to legal contentions, be they civil or criminal matters. DNA profiling (also called DNA typing or DNA fingerprinting) is a forensic techniques used to identify individuals by characteristics of their DNA in crime cases. DNA profiling can be use to resolve paternal and ancestral issues. This process was built mainly on the knowledge of two scientific breakthroughs. First is the Polymerase Chain Reaction (PCR) which was conceived by Kary Mullis in 1983 at Cetus Corporation, USA. Second is the Restriction Fragment Length Polymorphism (RFLP) analysis of repeated DNA sequences which was discovered by Professor Sir Alec Jeffreys in 1985 at the University of Leicester, UK. The strengths and limitations of the current and emerging forensic DNA profiling are the focus of this seminar. It is my expectation that the newly proposed synthetic human genome project will aid the strength of this process in the future.

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Dna Technology

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