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Biology	
  A2	
  
Unit	
  4	
  Revision	
  Notes	
  
	
  

AQA
	
  

	
  
Biology	
  Unit	
  4	
  –	
  3.4.1	
  
Definitions	
  
Word	
  
Ecosystem	
  

Definition	
  
An	
  ecosystem	
  is	
  made	
  up	
  of	
  all	
  the	
  interacting	
  biotic	
  
(living)	
  and	
  abiotic	
  (non-­‐living)	
  factors	
  in	
  a	
  specific	
  
area	
  	
  

Population	
  

Is	
  a	
  group	
  of	
  interbreeding	
  organisms	
  of	
  one	
  species	
  
in	
  a	
  habitat	
  

Community	
  

All	
  the	
  populations	
  of	
  different	
  organisms	
  in	
  a	
  given	
  
area	
  at	
  a	
  given	
  time	
  

Habitat	
  

Place	
  where	
  a	
  community	
  of	
  organisms	
  lives	
  (e.g.	
  
decaying	
  log)	
  

Ecological	
  Niche	
  

How	
  an	
  organism	
  fits	
  into	
  the	
  environment,	
  refers	
  
to	
  both	
  where	
  an	
  organism	
  lives	
  and	
  what	
  it	
  does	
  
there.	
  No	
  two	
  species	
  occupy	
  the	
  same	
  niche	
  

	
  

Investigating	
  populations	
  
Quadrats	
  
3	
  factors	
  to	
  consider:	
  
1. The	
  size	
  of	
  quadrat	
  to	
  use	
  –	
  this	
  will	
  depend	
  on	
  the	
  size	
  of	
  whatever	
  you’re	
  sampling	
  and	
  how	
  they	
  
are	
  distributed	
  within	
  the	
  sample	
  area	
  
2. The	
  number	
  of	
  quadrats	
  to	
  record	
  within	
  the	
  sample	
  area	
  –	
  for	
  questions	
  asking	
  about	
  this	
  10	
  or	
  
more	
  should	
  be	
  used	
  within	
  each	
  area	
  to	
  get	
  a	
  big	
  enough	
  sample	
  size,	
  basically	
  more	
  is	
  better	
  
3. The	
  position	
  of	
  each	
  quadrat	
  within	
  the	
  sample	
  area	
  –	
  random	
  sampling	
  should	
  be	
  used	
  
Random	
  sampling	
  
• This	
  is	
  used	
  to	
  prevent	
  bias	
  –	
  as	
  someone	
  might	
  pick	
  to	
  place	
  a	
  quadrat	
  where	
  there	
  is	
  a	
  large	
  
amount	
  of	
  clover	
  for	
  example	
  but	
  this	
  may	
  not	
  be	
  representative.	
  
You	
  should:	
  
•
•
•

Place	
  two	
  tape	
  measures	
  at	
  right	
  angles	
  along	
  two	
  sides	
  of	
  the	
  area	
  you’re	
  studying	
  
Obtain	
  co-­‐ordinates	
  using	
  a	
  random	
  numbers	
  table	
  
Place	
  quadrats	
  at	
  the	
  intersection	
  of	
  each	
  pair	
  of	
  co-­‐ordinates	
  

Systematic	
  sampling	
  using	
  transects	
  
• Transect	
  is	
  a	
  line	
  or	
  tape	
  
• This	
  can	
  be	
  used	
  more	
  effectively	
  than	
  quadrats	
  for	
  measuring	
  things	
  such	
  as	
  abundance	
  of	
  species	
  
as	
  you	
  enter	
  a	
  forest	
  or	
  measuring	
  the	
  abundance	
  of	
  species	
  comared	
  with	
  how	
  far	
  away	
  from	
  the	
  
sea	
  you	
  are	
  
They	
  can	
  be	
  used	
  in	
  two	
  ways:	
  
Any	
  organism	
  over	
  which	
  the	
  line	
  passes	
  is	
  recorded	
  
You	
  can	
  put	
  a	
  quadrat	
  down	
  every	
  however	
  many	
  meters	
  down	
  the	
  transect	
  

•
•

Measuring	
  abundance	
  
Sampling	
  is	
  used	
  to	
  get	
  a	
  measure	
  of	
  abundance.	
  This	
  is	
  the	
  number	
  of	
  individuals	
  of	
  a	
  species	
  within	
  a	
  
given	
  space.	
  Two	
  ways	
  of	
  measuring	
  this:	
  
•
•

Frequency	
  –	
  counting	
  individual	
  animals	
  or	
  plants	
  
Percentage	
  cover	
  –	
  an	
  estimate	
  of	
  the	
  area	
  within	
  the	
  quadrat	
  that	
  one	
  species	
  covers	
  

Mark-­‐release-­‐recapture	
  
This	
  is	
  carried	
  out	
  to	
  determine	
  the	
  population	
  size.	
  This	
  method	
  is	
  used:	
  
•
•
•
•

A	
  known	
  number	
  of	
  animals	
  are	
  caught	
  
These	
  are	
  marked	
  in	
  some	
  way	
  
These	
  are	
  then	
  released	
  back	
  into	
  the	
  community	
  
Later	
  more	
  animals	
  are	
  caught	
  and	
  the	
  number	
  of	
  marked	
  individuals	
  are	
  recorded	
  

Then	
  this	
  formula	
  is	
  used	
  to	
  determine	
  population	
  size	
  (needs	
  to	
  be	
  remembered	
  for	
  exam):	
  
𝑒𝑠𝑡𝑖𝑚𝑎𝑡𝑒𝑑  𝑝𝑜𝑝𝑢𝑙𝑎𝑡𝑖𝑜𝑛  𝑠𝑖𝑧𝑒
𝑛𝑢𝑚𝑏𝑒𝑟  𝑜𝑓  𝑖𝑛𝑑𝑖𝑣𝑖𝑑𝑢𝑎𝑙𝑠  𝑖𝑛  𝑠𝑎𝑚𝑝𝑙𝑒  1  ×  𝑛𝑢𝑚𝑏𝑒𝑟  𝑜𝑓  𝑖𝑛𝑑𝑖𝑣𝑖𝑑𝑢𝑎𝑙𝑠  𝑖𝑛  𝑠𝑎𝑚𝑝𝑙𝑒  2
=   
	
  
𝑛𝑢𝑚𝑏𝑒𝑟  𝑜𝑓  𝑚𝑎𝑟𝑘𝑒𝑑  𝑖𝑛𝑑𝑖𝑣𝑖𝑑𝑢𝑎𝑙𝑠  𝑖𝑛  𝑠𝑎𝑚𝑝𝑙𝑒  2
This	
  technique	
  relies	
  on	
  a	
  number	
  of	
  assumptions:	
  
•
•
•
•
•

The	
  marked	
  individuals	
  distribute	
  themselves	
  evenly	
  amongst	
  the	
  other	
  individuals	
  in	
  the	
  
population.	
  
No	
  deaths	
  or	
  births	
  in	
  the	
  population	
  
No	
  emigration	
  or	
  immigration	
  effecting	
  the	
  population	
  
The	
  method	
  of	
  marking	
  is	
  non-­‐toxic	
  to	
  the	
  individual	
  and	
  it	
  doesn’t	
  make	
  them	
  more	
  liable	
  to	
  
predation	
  
The	
  mark	
  isn’t	
  rubbed	
  off	
  or	
  lost	
  during	
  the	
  investigation	
  
Population	
  growth	
  curves	
  

	
  
1. Lag	
  phase	
  –	
  slow	
  growth	
  –	
  small	
  numbers	
  initially	
  so	
  reproduction	
  slow	
  –	
  organisms	
  are	
  getting	
  
used	
  to	
  the	
  conditions	
  
2. Log	
  phase	
  –	
  rapid	
  growth	
  and	
  optimum	
  conditions	
  
3. Stationary	
  phase	
  –	
  stable	
  state	
  –	
  no	
  population	
  growth	
  –	
  small	
  fluctuations	
  due	
  to	
  changes	
  in	
  
factors	
  such	
  as	
  food	
  supply	
  
	
  

Abiotic	
  factors	
  
•
•
•
•

Temperature	
  –	
  each	
  species	
  has	
  an	
  optimum	
  –	
  the	
  further	
  away	
  from	
  this	
  you	
  go	
  the	
  smaller	
  the	
  
population	
  that	
  can	
  be	
  supported	
  
Light	
  –	
  ultimate	
  source	
  of	
  energy	
  for	
  ecosystems	
  –	
  rate	
  of	
  photosynthesis	
  increases	
  as	
  light	
  
intensity	
  increases	
  –	
  this	
  allows	
  a	
  larger	
  primary	
  consumer	
  population	
  to	
  be	
  supported	
  
pH	
  –	
  this	
  effects	
  the	
  action	
  of	
  enzymes	
  –	
  a	
  population	
  of	
  organisms	
  is	
  larger	
  where	
  the	
  optimum	
  pH	
  
is	
  
water	
  and	
  humidity	
  –	
  where	
  water	
  is	
  scarce	
  populations	
  are	
  small	
  and	
  only	
  well	
  adapted	
  organisms	
  
survive	
  –	
  humidity	
  effects	
  the	
  later	
  loss	
  from	
  plants	
  (transpiration)	
  and	
  animals	
  in	
  dry	
  air	
  conditions	
  
only	
  those	
  individuals	
  with	
  adaptations	
  to	
  this	
  will	
  survive	
  

Competition	
  
Intraspecific	
  
•
•
•
•

Individuals	
  of	
  the	
  same	
  species	
  
Competing	
  for	
  resources	
  such	
  as	
  food,	
  space,	
  light	
  etc.	
  
Availability	
  of	
  the	
  resources	
  that	
  determines	
  population	
  size	
  
Lower	
  the	
  availability	
  smaller	
  the	
  population	
  size	
  and	
  vice	
  versa	
  

Interspecific	
  
•
•
•

Individuals	
  of	
  different	
  species	
  
Competing	
  for	
  resources	
  such	
  as	
  food,	
  space,	
  light	
  etc.	
  
Competitive	
  advantage	
  determines	
  which	
  population	
  will	
  grow	
  
•

If	
  conditions	
  remain	
  the	
  same	
  this	
  will	
  lead	
  to	
  the	
  complete	
  removal	
  of	
  one	
  species	
  as	
  they	
  can’t	
  
compete	
  in	
  this	
  niche	
  

Predation	
  
•

Occurs	
  when	
  one	
  organism	
  is	
  consumed	
  by	
  another	
  

Data	
  can	
  be	
  inaccurate	
  on	
  this	
  as	
  it	
  has	
  to	
  be	
  measured	
  in	
  the	
  wild	
  by	
  sampling	
  which	
  is	
  only	
  as	
  good	
  as	
  the	
  
methods	
  used.	
  None	
  of	
  these	
  methods	
  guarantee	
  complete	
  accuracy	
  so	
  caution	
  is	
  advised	
  with	
  any	
  data	
  
produced	
  this	
  way	
  

Predator-­‐prey	
  relationship	
  

	
  
•
•
•
•
•
•

Predators	
  eat	
  their	
  prey	
  therefore	
  reducing	
  the	
  population	
  size	
  of	
  the	
  prey	
  
With	
  fewer	
  prey	
  available	
  the	
  predators	
  are	
  in	
  greater	
  competition	
  with	
  each	
  other	
  for	
  the	
  
remaining	
  prey	
  
The	
  predator	
  population	
  is	
  reduced	
  as	
  some	
  individuals	
  are	
  unable	
  to	
  consume	
  enough	
  prey	
  to	
  
survive	
  and	
  reproduce	
  
With	
  fewer	
  predators	
  left	
  less	
  prey	
  is	
  consumed	
  
The	
  prey	
  population	
  increases	
  
More	
  prey	
  are	
  now	
  available	
  so	
  predator	
  population	
  increases	
  

This	
  is	
  a	
  cycle	
  and	
  carries	
  on	
  and	
  as	
  the	
  graph	
  shows	
  

The	
  human	
  population	
  
Factors	
  effecting	
  population	
  size	
  
•
•
•
•

Birth	
  rate	
  
Death	
  rate	
  
Immigration	
  
Emigration	
  

𝑝𝑜𝑝𝑢𝑙𝑎𝑡𝑖𝑜𝑛  𝑔𝑟𝑜𝑤𝑡ℎ = 𝑏𝑖𝑟𝑡ℎ𝑠 + 𝑖𝑚𝑚𝑖𝑔𝑟𝑎𝑡𝑖𝑜𝑛 − (𝑑𝑒𝑎𝑡ℎ𝑠 + 𝑒𝑚𝑖𝑔𝑟𝑎𝑡𝑖𝑜𝑛)	
  
𝑝𝑒𝑟𝑐𝑒𝑛𝑡𝑎𝑔𝑒  𝑝𝑜𝑝𝑢𝑙𝑎𝑡𝑖𝑜𝑛  𝑔𝑟𝑜𝑤𝑡ℎ =   

𝑝𝑜𝑝𝑢𝑙𝑎𝑡𝑖𝑜𝑛  𝑐ℎ𝑎𝑛𝑔𝑒  𝑑𝑢𝑟𝑖𝑛𝑔  𝑡ℎ𝑒  𝑝𝑒𝑟𝑖𝑜𝑑
  ×100%	
  
𝑝𝑜𝑝𝑢𝑙𝑎𝑡𝑖𝑜𝑛  𝑎𝑡  𝑡ℎ𝑒  𝑠𝑡𝑎𝑟𝑡  𝑜𝑓  𝑡ℎ𝑒  𝑝𝑒𝑟𝑖𝑜𝑑

Factors	
  effecting	
  birth	
  rates	
  
•
•
•
•
•

Economic	
  conditions	
  
Cultural	
  and	
  religious	
  backgrounds	
  
Social	
  pressures	
  and	
  conditions	
  
Birth	
  control	
  
Political	
  factors	
  

𝐵𝑖𝑟𝑡ℎ  𝑟𝑎𝑡𝑒 =   

𝑡𝑜𝑡𝑎𝑙  𝑛𝑢𝑚𝑏𝑒𝑟  𝑜𝑓  𝑏𝑖𝑟𝑡ℎ𝑠  𝑝𝑒𝑟  𝑦𝑒𝑎𝑟
×1000	
  
𝑡𝑜𝑡𝑎𝑙  𝑝𝑜𝑝𝑢𝑙𝑎𝑡𝑖𝑜𝑛  𝑖𝑛  𝑡ℎ𝑒  𝑠𝑎𝑚𝑒  𝑦𝑒𝑎𝑟

Factors	
  effecting	
  death	
  rate	
  
•
•
•
•
•
•
•

Age	
  
Life	
  expectancy	
  
Food	
  supply	
  
Availability	
  of	
  safe	
  drinking	
  water	
  and	
  effective	
  sanitation	
  
Access	
  to	
  medical	
  care	
  
Natural	
  disasters	
  
War	
  

𝐷𝑒𝑎𝑡ℎ  𝑟𝑎𝑡𝑒 =   

𝑡𝑜𝑡𝑎𝑙  𝑛𝑢𝑚𝑏𝑒𝑟  𝑜𝑓  𝑑𝑒𝑎𝑡ℎ𝑠  𝑝𝑒𝑟  𝑦𝑒𝑎𝑟
×1000	
  
𝑡𝑜𝑡𝑎𝑙  𝑝𝑜𝑝𝑢𝑙𝑎𝑡𝑖𝑜𝑛  𝑖𝑛  𝑡ℎ𝑒  𝑠𝑎𝑚𝑒  𝑦𝑒𝑎𝑟

Demographic	
  Transition	
  

	
  
(©	
  Nelson	
  Thornes	
  A2	
  AQA	
  Biology	
  text	
  book)	
  
This	
  summarises	
  a	
  pattern	
  that	
  different	
  countries	
  have	
  gone	
  through	
  as	
  they	
  have	
  developed	
  
Age	
  population	
  pyramids	
  
These	
  fit	
  into	
  the	
  demographic	
  transition	
  stages	
  based	
  on	
  the	
  shape	
  of	
  the	
  pyramid:	
  
Stage	
  
1	
  

Pyramid	
  

Shape	
  explanation	
  
Bigger	
  bars	
  at	
  the	
  bottom	
  
and	
  less	
  at	
  the	
  top	
  as	
  high	
  
birth	
  rate	
  so	
  lots	
  of	
  
children	
  but	
  also	
  high	
  
death	
  rate	
  so	
  less	
  older	
  
people	
  

	
  
2	
  

Still	
  high	
  birth	
  rate	
  so	
  big	
  
bars	
  at	
  the	
  bottom	
  but	
  
decreasing	
  death	
  rate	
  so	
  
more	
  in	
  the	
  middle	
  and	
  
more	
  people	
  living	
  longer.	
  
	
  
	
  
	
   	
  
	
  
	
  
	
  
	
  
	
  
	
  

3	
  

	
  

Low	
  death	
  rate	
  so	
  bigger	
  
bars	
  in	
  the	
  middle	
  and	
  
more	
  people	
  reaching	
  the	
  
top	
  of	
  the	
  pyramid.	
  Birth	
  
rate	
  is	
  decreasing	
  so	
  less	
  
at	
  the	
  bottom	
  of	
  the	
  
pyramid	
  

	
  
4	
  

Low	
  birth	
  rate	
  and	
  death	
  
rate	
  so	
  smaller	
  bars	
  at	
  the	
  
bottom	
  and	
  bigger	
  bars	
  in	
  
the	
  middle	
  also	
  more	
  
people	
  reaching	
  the	
  top	
  so	
  
larger	
  bars	
  there	
  	
  

	
  
	
  
	
  

	
  
Biology	
  Unit	
  4	
  –	
  Section	
  3.4.2	
  
Why	
  do	
  organisms	
  need	
  energy	
  
•
•
•
•
•
•

Metabolism	
  –	
  all	
  the	
  reactions	
  that	
  take	
  place	
  in	
  living	
  organisms	
  involve	
  energy	
  
Movement	
  
Active	
  transport	
  
Maintenance,	
  repair	
  and	
  division	
  of	
  cells	
  
Production	
  of	
  enzymes	
  and	
  hormones	
  
Maintenance	
  of	
  body	
  temperature	
  
	
  

Hydrolysis	
  (ATP	
  +	
  Water)	
  
	
  

ATP	
  

ADP	
  +	
  Pi	
  
	
  

	
  

Energy	
  from	
  respiration	
  

	
  
	
  

Synthesis	
  of	
  ATP	
  from	
  ADP	
  
This	
  requires	
  the	
  addition	
  of	
  a	
  phosphate	
  to	
  ADP.	
  There	
  are	
  3	
  ways	
  this	
  occurs:	
  
•
•
•

PHOTOPHOSOHORYLATION	
  –	
  This	
  occurs	
  in	
  chlorophyll	
  containing	
  plant	
  cells	
  during	
  photosynthesis	
  
OXIDATIVE	
  PHOSPHORYLATION	
  –	
  Which	
  occurs	
  in	
  the	
  mitochondria	
  of	
  plant	
  and	
  animal	
  cells	
  during	
  
the	
  process	
  of	
  the	
  electron	
  transport	
  chain	
  
SUBSTRATE	
  –	
  LEVEL	
  PHOSPHORYLATION	
  -­‐	
  	
  Occurs	
  in	
  plant	
  and	
  animal	
  cells	
  when	
  donor	
  molecules	
  
donate	
  phosphate	
  to	
  the	
  ADP	
  to	
  make	
  ATP	
  like	
  in	
  the	
  formation	
  of	
  pyruvate	
  

ATP	
  IS	
  AN	
  IMMEDIATE	
  ENERGY	
  SOURCE	
  AND	
  ISN’T	
  A	
  GOOD	
  LONG	
  TERM	
  STORE	
  OF	
  ENERGY.	
  IT	
  RELEASES	
  ENERGY	
  IN	
  
MANAGABLE	
  Q UANTITIES	
   F OR	
   C ELL	
   R EACTIONS	
   A ND	
   C AN	
   Q UICKLY	
   B E	
   R EFORMED	
   M AKING	
   I T	
   A 	
   G OOD	
   IMMEDIATE	
  
ENERGY	
  SOURCE	
  
	
  

	
  
Biology	
  Unit	
  4	
  –	
  Section	
  3.4.3	
  
Overview	
  of	
  photosynthesis	
  
1. Capturing	
  of	
  light	
  energy	
  by	
  chloroplast	
  pigments	
  
2. Light	
  dependent	
  reaction	
  
3. Light	
  independent	
  reaction	
  

Structure	
  of	
  a	
  chloroplast	
  

	
  
(image	
  from	
  passscience.blogspot.com)	
  
•
•

Light	
  dependent	
  reaction	
  takes	
  place	
  in	
  the	
  thylakoids	
  
Light	
  independent	
  reaction	
  takes	
  place	
  in	
  the	
  stroma	
  

Light	
  dependent	
  reaction	
  
Two	
  purposes:	
  
•
•

Add	
  phosphate	
  to	
  ADP	
  making	
  ATP	
  (photophosphorylation)	
  
Split	
  water	
  into	
  H+	
  ions,	
  electrons	
  and	
  Oxygen	
  (photolysis)	
  

Oxidation	
  and	
  reduction	
  
Oxidation	
  –	
  The	
  loss	
  of	
  electrons,	
  the	
  loss	
  of	
  hydrogen	
  or	
  the	
  gain	
  of	
  oxygen	
  
Reduction	
  –	
  The	
  gain	
  of	
  electrons,	
  the	
  gain	
  of	
  hydrogen	
  or	
  the	
  loss	
  of	
  oxygen	
  
Wordy	
  Explanation	
  of	
  what	
  happens	
  
• When	
  chlorophyll	
  molecules	
  absorb	
  light	
  energy	
  a	
  pair	
  it	
  boosts	
  the	
  energy	
  of	
  a	
  a	
  pair	
  of	
  electrons	
  
which	
  raises	
  them	
  to	
  a	
  higher	
  energy	
  level	
  
• These	
  electrons	
  have	
  so	
  much	
  energy	
  that	
  they	
  leave	
  the	
  chlorophyll	
  molecule	
  
• They	
  are	
  taken	
  up	
  by	
  an	
  electron	
  carrier	
  
• The	
  pair	
  of	
  electrons	
  are	
  now	
  passed	
  along	
  a	
  series	
  of	
  electron	
  carriers	
  in	
  oxidation	
  reduction	
  
reactions	
  (located	
  in	
  the	
  membrane	
  of	
  the	
  thylakoids)	
  
• Each	
  carrier	
  is	
  at	
  a	
  slightly	
  lower	
  energy	
  level	
  than	
  the	
  last	
  and	
  the	
  electrons	
  lose	
  energy	
  
• This	
  energy	
  is	
  used	
  to	
  add	
  a	
  phosphate	
  to	
  ADP	
  to	
  make	
  ATP	
  
• The	
  photolysis	
  of	
  water	
  also	
  happens	
  
• This	
  is	
  due	
  to	
  chlorophyll	
  molecules	
  losing	
  two	
  electrons	
  so	
  these	
  need	
  to	
  be	
  replaced	
  
•

These	
  replacement	
  electrons	
  are	
  provided	
  by	
  the	
  splitting	
  of	
  water	
  molecules	
  as	
  shown	
  below:	
  

2𝐻! 𝑂 → 4𝐻! + 4𝑒 ! + 𝑂! 	
  
•

The	
  H+	
  is	
  taken	
  up	
  by	
  NADP	
  to	
  form	
  reduced	
  NADP	
  (or	
  NADPH2)	
  this	
  then	
  enters	
  the	
  light	
  
independent	
  reaction	
  

Diagrammatic	
  explanation	
  

	
  
(©	
  Nelson	
  Thornes	
  AQA	
  A2	
  Biology	
  Text	
  Book)	
  

Light	
  independent	
  reaction	
  
•
•

Products	
  of	
  light	
  dependent	
  reaction	
  (reduced	
  NADP	
  and	
  ATP)	
  are	
  used	
  
Takes	
  place	
  in	
  the	
  stroma	
  of	
  the	
  chloroplasts	
  

Wordy	
  explanation	
  
1. Carbon	
  dioxide	
  diffuses	
  from	
  the	
  air	
  eventually	
  into	
  the	
  stroma	
  of	
  the	
  chloroplast	
  
2. Here	
  it	
  combines	
  with	
  the	
  5	
  carbon	
  Ribulose	
  Bisphosphate	
  (RuBP)	
  
3. This	
  produces	
  two	
  molecules	
  of	
  glycerate-­‐3-­‐phosphate	
  (GP)	
  
4. ATP	
  and	
  reduced	
  NADP	
  from	
  the	
  light	
  dependent	
  reaction	
  are	
  then	
  used	
  to	
  reduce	
  GP	
  into	
  triose	
  
phosphate	
  (TP)	
  
5. NADP	
  (non-­‐reduced)	
  is	
  reformed	
  and	
  goes	
  back	
  into	
  the	
  light	
  dependent	
  reaction	
  to	
  accept	
  more	
  
hydrogen	
  
6. Some	
  triose	
  phosphate	
  molecules	
  are	
  used	
  to	
  make	
  other	
  useful	
  substances	
  such	
  as	
  glucose	
  
7. Most	
  triose	
  phosphate	
  molecules	
  are	
  used	
  to	
  regenerate	
  RuBP	
  using	
  ATP	
  
Diagram	
  

	
  
(©	
  Nelson	
  Thornes	
  AQA	
  A2	
  Biology	
  Text	
  Book)	
  

Limiting	
  factors	
  on	
  photosynthesis	
  
At	
  any	
  given	
  moment	
  the	
  rate	
  is	
  limited	
  by	
  the	
  factor	
  that	
  is	
  at	
  its	
  least	
  favourable	
  value	
  

	
  
Factors	
  that	
  limit	
  photosynthesis	
  
•
•
•

Light	
  intensity	
  
Temperature	
  	
  
CO2	
  Concentration	
  

Temperature	
  is	
  a	
  factor	
  as	
  it’s	
  an	
  enzyme	
  controlled	
  reaction	
  so	
  higher	
  temperature	
  gives	
  the	
  molecules	
  
more	
  energy	
  and	
  therefore	
  they	
  collide	
  with	
  the	
  right	
  amount	
  of	
  energy	
  with	
  the	
  enzyme	
  and	
  form	
  more	
  
enzyme	
  substrate	
  complexes.	
  
	
  
	
  

	
  
Biology	
  Unit	
  4	
  –	
  Section	
  3.4.4	
  
Aerobic	
  respiration	
  
4	
  stages:	
  
•
•
•
•

Glycolysis	
  
Link	
  reaction	
  
Krebs	
  Cycle	
  
Electron	
  Transport	
  Chain	
  

Glycolysis	
  
• Phosphorylation	
  of	
  Glucose	
  
• Splitting	
  of	
  phosphorylated	
  glucose	
  
• Oxidation	
  of	
  triose	
  phosphate	
  (by	
  removal	
  of	
  hydrogen)	
  
• Hydrogen	
  accepted	
  by	
  NAD	
  to	
  form	
  NADH2	
  
• Production	
  of	
  ATP	
  
• Formation	
  of	
  pyruvate	
  

	
  

	
  
Link	
  Reaction	
  
• Pyruvate	
  oxidised	
  by	
  removal	
  of	
  hydrogen	
  
• Hydrogen	
  accepted	
  by	
  NAD	
  to	
  form	
  NADH2	
  
• De-­‐carboxylation	
  occurs	
  producing	
  carbon	
  dioxide	
  
• Acetyl	
  group	
  formed	
  (2-­‐carbon)	
  
• Combines	
  with	
  coenzyme	
  A	
  to	
  form	
  the	
  2	
  carbon	
  acetylcoenzyme	
  A	
  

	
  
Krebs	
  Cycle	
  
• Acetylcoenzyme	
  A	
  feeds	
  into	
  this	
  from	
  the	
  link	
  reaction	
  
• Acetylcoenzyme	
  A	
  combines	
  with	
  a	
  4	
  carbon	
  compound	
  to	
  form	
  a	
  6	
  carbon	
  compound	
  
• This	
  then	
  undergoes	
  2	
  decarboxylation’s	
  removing	
  two	
  molecules	
  of	
  carbon	
  dioxide	
  
• It	
  also	
  reduces	
  two	
  hydrogen	
  carriers:	
  NAD	
  and	
  FAD	
  forming	
  reduced	
  NAD	
  and	
  reduced	
  FAD	
  
• This	
  then	
  is	
  the	
  4	
  carbon	
  compound	
  needed	
  to	
  combine	
  with	
  another	
  acetylcoenzyme	
  A	
  molecule	
  
and	
  so	
  it	
  keeps	
  on	
  going	
  

	
  
 
Electron	
  transport	
  chain	
  
• Hydrogen	
  atoms	
  collected	
  by	
  coenzymes	
  NAD	
  and	
  FAD	
  are	
  used	
  
• These	
  are	
  split	
  into	
  electrons	
  and	
  protons	
  
• The	
  electrons	
  get	
  passed	
  down	
  the	
  electron	
  transport	
  chain	
  where	
  as	
  they	
  are	
  passed	
  from	
  carrier	
  
to	
  carrier	
  they	
  lose	
  energy	
  
• This	
  energy	
  is	
  used	
  to	
  combine	
  a	
  phosphate	
  with	
  ADP	
  to	
  make	
  ATP	
  
• The	
  protons	
  are	
  pumped	
  into	
  the	
  inter-­‐membrane	
  space	
  of	
  the	
  mitochondria	
  (the	
  space	
  between	
  
the	
  cristae	
  and	
  the	
  outer	
  membrane	
  
• As	
  they	
  accumulate	
  here	
  they	
  diffuse	
  back	
  through	
  special	
  channels	
  	
  
• At	
  the	
  end	
  of	
  the	
  chain	
  the	
  electrons	
  combine	
  with	
  these	
  protons	
  and	
  oxygen	
  to	
  form	
  water	
  

	
  
	
  

Anaerobic	
  Respiration	
  
•
•
•
•
•

When	
  there	
  is	
  little	
  or	
  no	
  oxygen	
  neither	
  the	
  krebs	
  cycle	
  or	
  electron	
  transport	
  chain	
  can	
  take	
  place	
  
Only	
  glycolysis	
  can	
  
So	
  lots	
  of	
  pyruvate	
  produced	
  in	
  order	
  to	
  produce	
  the	
  2	
  ATP	
  molecules	
  glycolysis	
  yields	
  (net	
  yield)	
  
NAD	
  must	
  be	
  regenerated	
  so	
  pyruvate	
  accepts	
  this	
  hydrogen	
  
However	
  this	
  happens	
  differently	
  in	
  animals	
  and	
  plants	
  

In	
  plants	
  and	
  some	
  microorganisms	
  
	
  
𝑝𝑦𝑟𝑢𝑣𝑎𝑡𝑒 + 𝑟𝑒𝑑𝑢𝑐𝑒𝑑  𝑁𝐴𝐷   → 𝑒𝑡ℎ𝑎𝑛𝑜𝑙 + 𝑐𝑎𝑟𝑏𝑜𝑛  𝑑𝑖𝑜𝑥𝑖𝑑𝑒 + 𝑁𝐴𝐷	
  
 

	
  

In	
  animals	
  
𝑝𝑦𝑟𝑢𝑣𝑎𝑡𝑒 + 𝑟𝑒𝑑𝑢𝑐𝑒𝑑  𝑁𝐴𝐷   → 𝑙𝑎𝑐𝑡𝑎𝑡𝑒   𝑙𝑎𝑐𝑡𝑖𝑐  𝑎𝑐𝑖𝑑 + 𝑁𝐴𝐷	
  
In	
  both	
  cases	
  this	
  is	
  very	
  inefficient	
  as	
  only	
  2	
  ATP	
  molecules	
  are	
  produced	
  by	
  glycolysis	
  and	
  
there	
  is	
  a	
  much	
  greater	
  quantity	
  produced	
  by	
  Aerobic	
  respiration	
  
	
  

	
  
Biology	
  Unit	
  4	
  –	
  Section	
  3.4.5	
  
Food	
  Chains	
  and	
  Food	
  Webs	
  
Producers	
  
Photosynthetic	
  organisms	
  that	
  manufacture	
  organic	
  substances	
  using	
  light	
  energy	
  
Consumers	
  
Organisms	
  that	
  obtain	
  their	
  energy	
  by	
  feeding	
  on	
  other	
  organisms.	
  Those	
  that	
  directly	
  eat	
  plants	
  are	
  called	
  
primary	
  consumers.	
  The	
  animals	
  eating	
  those	
  organisms	
  are	
  called	
  secondary	
  consumers	
  and	
  so	
  on.	
  Not	
  
normally	
  more	
  than	
  4	
  consumers	
  in	
  a	
  food	
  chain	
  
Decomposers	
  
When	
  producers	
  and	
  consumers	
  die	
  these	
  organisms	
  make	
  the	
  energy	
  contained	
  in	
  the	
  other	
  organisms	
  
available	
  for	
  the	
  food	
  chain	
  again	
  by	
  breaking	
  down	
  the	
  producer/consumer.	
  These	
  nutrients	
  can	
  then	
  be	
  
absorbed	
  by	
  plants	
  and	
  used	
  in	
  the	
  food	
  chain	
  
Food	
  webs	
  
In	
  reality	
  many	
  animals	
  don’t	
  rely	
  on	
  a	
  single	
  food	
  source	
  and	
  in	
  a	
  habitat	
  many	
  food	
  chains	
  link	
  together	
  
forming	
  a	
  food	
  web	
  with	
  lots	
  of	
  different	
  organisms	
  interacting	
  

	
  

Energy	
  Losses	
  in	
  food	
  chains	
  
Producers:	
  
• Over	
  90%	
  of	
  the	
  suns	
  energy	
  reflected	
  back	
  into	
  space	
  by	
  clouds	
  and	
  dust	
  or	
  absorbed	
  by	
  the	
  
atmosphere	
  
• Not	
  all	
  wavelengths	
  of	
  light	
  can	
  be	
  absorbed	
  and	
  used	
  for	
  photosynthesis	
  
• Light	
  may	
  not	
  fall	
  of	
  a	
  chlorophyll	
  molecule	
  
• A	
  factor	
  such	
  as	
  low	
  carbon	
  dioxide	
  levels	
  may	
  limit	
  the	
  rate	
  of	
  photosynthesis	
  
Formula	
  to	
  work	
  out	
  NP	
  (net	
  production)	
  
𝑁𝑒𝑡  𝑝𝑟𝑜𝑑𝑢𝑐𝑡𝑖𝑜𝑛 = 𝑔𝑟𝑜𝑠𝑠  𝑝𝑟𝑜𝑑𝑢𝑐𝑡𝑖𝑜𝑛 − 𝑟𝑒𝑠𝑝𝑖𝑟𝑎𝑡𝑜𝑟𝑦  𝑙𝑜𝑠𝑠𝑒𝑠	
  
Losses	
  by	
  primary	
  consumers:	
  
• Some	
  of	
  the	
  organism	
  isn’t	
  eaten	
  
•
•
•

Some	
  parts	
  can’t	
  be	
  digested	
  
Energy	
  lost	
  in	
  excretory	
  products	
  such	
  as	
  urine	
  
Energy	
  losses	
  due	
  to	
  heat	
  loss	
  

Why	
  are	
  food	
  chains	
  short?	
  
• Most	
  food	
  chains	
  only	
  have	
  4	
  or	
  5	
  trophic	
  levels	
  as	
  there	
  isn’t	
  enough	
  energy	
  available	
  to	
  support	
  a	
  
population	
  at	
  another	
  level	
  
• The	
  total	
  mass	
  of	
  the	
  organisms	
  in	
  a	
  particular	
  place	
  (biomass)	
  is	
  less	
  at	
  higher	
  trophic	
  levels	
  
• The	
  total	
  amount	
  of	
  energy	
  stored	
  is	
  less	
  at	
  each	
  level	
  as	
  you	
  move	
  up	
  the	
  food	
  chain	
  

	
  Calculating	
  efficiency	
  of	
  energy	
  transfer	
  
𝑒𝑛𝑒𝑟𝑔𝑦  𝑡𝑟𝑎𝑛𝑠𝑓𝑒𝑟 =   

𝑒𝑛𝑒𝑟𝑔𝑦  𝑎𝑣𝑎𝑖𝑙𝑎𝑏𝑙𝑒  𝑎𝑓𝑡𝑒𝑟  𝑡ℎ𝑒  𝑡𝑎𝑛𝑠𝑓𝑒𝑟
×100%	
  
𝑒𝑛𝑒𝑟𝑔𝑦  𝑎𝑣𝑎𝑖𝑙𝑎𝑏𝑙𝑒  𝑏𝑒𝑓𝑜𝑟𝑒  𝑡ℎ𝑒  𝑡𝑟𝑎𝑛𝑠𝑓𝑒𝑟

Ecological	
  pyramids	
  
Numbers	
  
• The	
  actual	
  numbers	
  of	
  organisms	
  proportional	
  to	
  each	
  other	
  
• Drawbacks:	
  
o No	
  account	
  is	
  taken	
  of	
  size	
  so	
  a	
  large	
  oak	
  tree	
  is	
  just	
  1	
  even	
  though	
  lots	
  of	
  aphids	
  can	
  feed	
  
on	
  it	
  
o This	
  will	
  create	
  an	
  inverted	
  pyramid	
  shape	
  
o Number	
  of	
  individuals	
  can	
  be	
  so	
  great	
  that	
  it	
  is	
  impossible	
  to	
  represent	
  them	
  accurately	
  on	
  
the	
  same	
  scale	
  
Biomass	
  
• Measure	
  of	
  the	
  total	
  mass	
  of	
  plants/animals	
  in	
  a	
  particular	
  place	
  
• More	
  reliable	
  and	
  quantitative	
  
• Only	
  the	
  organisms	
  present	
  at	
  a	
  particular	
  time	
  are	
  measured	
  so	
  this	
  doesn’t	
  take	
  into	
  account	
  
seasonal	
  differences	
  
Energy	
  
• Most	
  accurate	
  representation	
  of	
  energy	
  flow	
  through	
  an	
  ecosystem	
  
• This	
  measures	
  the	
  energy	
  stored	
  in	
  organisms	
  
• Collecting	
  data	
  however	
  can	
  be	
  difficult	
  and	
  complex	
  
• Always	
  a	
  pyramid	
  shape	
  

How	
  productivity	
  is	
  effected	
  by	
  farming	
  practices	
  
Fertilisers	
  
Fertilisers	
  are	
  substances	
  farmers	
  spread	
  on	
  the	
  soil	
  to	
  replace	
  nutrients,	
  normally	
  nitrogen,	
  which	
  plants	
  
get	
  from	
  the	
  soil.	
  
Natural	
  fertilisers	
  
Natural	
  fertilisers	
  are	
  the	
  faeces	
  of	
  animals	
  which	
  can	
  be	
  spread	
  onto	
  the	
  soil	
  to	
  increase	
  the	
  nutrients	
  
available	
  to	
  the	
  plants.	
  The	
  advantages	
  are	
  that	
  this	
  is	
  free	
  and	
  readily	
  available	
  to	
  the	
  farmer,	
  however	
  the	
  
disadvantage	
  is	
  that	
  it	
  has	
  to	
  be	
  left	
  in	
  the	
  soil	
  to	
  rot	
  down	
  so	
  that	
  the	
  nutrients	
  are	
  available	
  to	
  the	
  plants,	
  
which	
  takes	
  time.	
  They	
  may	
  also	
  contain	
  pathogens	
  which	
  can	
  be	
  harmful	
  to	
  the	
  plant	
  
Artificial	
  fertilisers	
  
Artificial	
  fertilisers	
  contain	
  nitrogen	
  compounds	
  such	
  as	
  ammonia	
  which	
  are	
  spread	
  onto	
  the	
  soil	
  normally	
  
in	
  soluble	
  granules	
  to	
  increase	
  nitrogen,	
  and	
  other	
  nutrients,	
  concentrations	
  in	
  the	
  soil.	
  The	
  advantages	
  are	
  
that	
  the	
  nitrogen	
  is	
  readily	
  available	
  for	
  plants	
  to	
  take	
  up	
  and	
  use.	
  Farmers	
  can	
  also	
  buy	
  types	
  which	
  have	
  
special	
  controlled	
  release	
  technology	
  which	
  means	
  there	
  is	
  a	
  constant	
  stable	
  nitrogen	
  content	
  going	
  into	
  
the	
  soil.	
  The	
  disadvantages	
  are	
  the	
  cost,	
  as	
  the	
  fertiliser	
  is	
  very	
  expensive	
  whereas	
  natural	
  fertilisers	
  are	
  
free.	
  Also	
  after	
  long	
  term	
  use	
  as	
  the	
  fertiliser	
  doesn’t	
  replace	
  the	
  trace	
  mineral	
  content	
  in	
  the	
  soil,	
  these	
  
can	
  run	
  out	
  and	
  aren’t	
  present	
  in	
  the	
  crop	
  or	
  the	
  fruit	
  so	
  humans	
  or	
  animals	
  aren’t	
  consuming	
  these	
  trace	
  
elements	
  which	
  can	
  be	
  bad	
  for	
  health.	
  
Fertilisers	
  add	
  nutrients	
  to	
  the	
  soil	
  to	
  help	
  increase	
  the	
  productivity	
  of	
  plants	
  
Pesticides	
  
There	
  are	
  two	
  types,	
  chemical	
  and	
  biological,	
  both	
  do	
  the	
  job	
  of	
  killing	
  pests	
  which	
  feed	
  on	
  food	
  crops	
  and	
  
fruit	
  which	
  ultimately	
  can	
  mean	
  that	
  farmers	
  don’t	
  get	
  the	
  maximum	
  yield	
  from	
  their	
  crops.	
  
A	
  pesticide	
  should	
  be:	
  
•
•
•
•

Specific	
  –	
  only	
  target	
  the	
  plant/insect/fungus	
  it	
  is	
  supposed	
  to	
  not	
  the	
  crop	
  or	
  any	
  other	
  organisms	
  
Biodegrade	
  –	
  so	
  once	
  it	
  has	
  been	
  used	
  it	
  doesn’t	
  go	
  into	
  the	
  soil	
  and	
  kill	
  the	
  crop,	
  however	
  it	
  also	
  
needs	
  to	
  have	
  a	
  long	
  shelf	
  life	
  
Cost-­‐effective	
  –	
  developing	
  a	
  pesticide	
  costs	
  a	
  lot	
  and	
  new	
  pesticides	
  only	
  are	
  effective	
  for	
  a	
  short	
  
length	
  of	
  time.	
  
Not	
  accumulate	
  –	
  so	
  it	
  doesn’t	
  build	
  up	
  in	
  the	
  food	
  chain	
  and	
  cause	
  problems	
  for	
  other	
  organisms	
  

Biological	
  control	
  
This	
  is	
  using	
  the	
  predator	
  of	
  the	
  pest	
  to	
  control	
  the	
  numbers	
  of	
  the	
  pest.	
  Its	
  advantages	
  are:	
  
•
•
•

Very	
  specific	
  
Once	
  introduced	
  the	
  predator	
  breeds	
  so	
  keeps	
  numbers	
  up	
  so	
  has	
  a	
  long	
  term	
  effect	
  
Pests	
  can’t	
  become	
  resistant	
  

The	
  ideal	
  situation	
  is	
  for	
  the	
  predator	
  to	
  exist	
  in	
  balance	
  with	
  the	
  pest	
  keeping	
  the	
  pest	
  at	
  a	
  level	
  where	
  it	
  
has	
  no	
  or	
  little	
  effect.	
  
	
  
There	
  are	
  however	
  some	
  disadvantages	
  with	
  this	
  method	
  these	
  are:	
  
•
•

They	
  do	
  not	
  act	
  as	
  quickly,	
  as	
  the	
  predator	
  has	
  to	
  build	
  up	
  its	
  numbers	
  so	
  there	
  is	
  a	
  lag	
  between	
  
introducing	
  the	
  predator	
  and	
  seeing	
  a	
  significant	
  drop	
  in	
  	
  the	
  pest	
  numbers	
  
The	
  predator	
  may	
  become	
  a	
  pest	
  for	
  example	
  if	
  there	
  are	
  few	
  natural	
  predators	
  to	
  it	
  or	
  as	
  the	
  pest	
  
population	
  decreases	
  it	
  may	
  use	
  the	
  crops	
  as	
  a	
  food	
  source.	
  

Integrated	
  systems	
  
Integrated	
  control	
  involves:	
  
•
•
•

Choosing	
  animal	
  or	
  plant	
  varieties	
  which	
  are	
  as	
  pest	
  resistant	
  as	
  possible	
  
Managing	
  the	
  environment	
  to	
  provide	
  habitats	
  for	
  natural	
  pest	
  controlling	
  organisms	
  to	
  live	
  
Regularly	
  checking	
  crops	
  for	
  signs	
  of	
  pest	
  activity	
  
•
•
•

Removing	
  pest	
  mechanically	
  (hands,	
  vacuum,	
  making	
  barriers)	
  
Using	
  biological	
  agents	
  
Only	
  using	
  chemical	
  pesticides	
  as	
  a	
  last	
  resort	
  

All	
  pests	
  damage	
  or	
  compete	
  with	
  plants	
  or	
  animals	
  leading	
  to	
  reduced	
  productivity	
  
Intensive	
  rearing	
  of	
  livestock	
  
Intensive	
  farming	
  is	
  about	
  converting	
  the	
  smallest	
  possible	
  amount	
  of	
  food	
  energy	
  into	
  the	
  largest	
  possible	
  
amount	
  of	
  animal	
  biomass.	
  This	
  is	
  achieved	
  by	
  minimise	
  the	
  energy	
  lost	
  by	
  animals	
  during	
  their	
  lifetime.	
  
Ways	
  in	
  which	
  this	
  is	
  achieved:	
  
•
•
•
•
•
•
	
  

Movement	
  is	
  restricted	
  –	
  less	
  energy	
  used	
  in	
  muscle	
  contraction	
  
Keeping	
  the	
  environment	
  warm	
  (for	
  warm	
  blooded	
  animals)	
  –	
  reduced	
  heat	
  loss	
  from	
  body	
  
Feeding	
  controlled	
  –	
  animals	
  receive	
  the	
  optimum	
  amount	
  and	
  type	
  of	
  food	
  for	
  maximum	
  growth	
  
Predators	
  are	
  excluded	
  –	
  no	
  loss	
  to	
  other	
  organisms	
  
Selective	
  breeding	
  –	
  produces	
  animals	
  which	
  are	
  the	
  most	
  efficient	
  at	
  converting	
  the	
  food	
  they	
  eat	
  
into	
  biomass	
  
Using	
  hormones	
  to	
  increase	
  growth	
  rates	
  
	
  
Biology	
  Unit	
  4	
  –	
  Section	
  3.4.6	
  
See	
  pages	
  at	
  the	
  end	
  of	
  the	
  carbon	
  and	
  nitrogen	
  cycle	
  diagrams,	
  as	
  these	
  are	
  the	
  first	
  bit	
  of	
  
this	
  section.	
  Also	
  see	
  section	
  3.4.5	
  first	
  as	
  there	
  is	
  some	
  overlap	
  with	
  the	
  use	
  of	
  fertilisers	
  

Effects	
  of	
  nitrogen	
  fertilisers	
  
Reduced	
  species	
  diversity	
  
This	
  is	
  because	
  nitrogen	
  rich	
  soils	
  favour	
  the	
  growth	
  of	
  fast	
  growing	
  species,	
  these	
  out	
  compete	
  many	
  
other	
  species	
  which	
  causes	
  these	
  other	
  species	
  to	
  die	
  as	
  a	
  result	
  
Leaching	
  
This	
  is	
  a	
  process	
  where	
  nutrients	
  are	
  removed	
  from	
  the	
  soil.	
  Rain	
  water	
  will	
  dissolve	
  any	
  soluble	
  nutrients	
  
and	
  carry	
  them	
  deep	
  into	
  the	
  soil	
  eventually	
  beyond	
  the	
  reach	
  of	
  the	
  plant	
  roots.	
  These	
  eventually	
  find	
  
their	
  way	
  into	
  water	
  courses.	
  They	
  can	
  have	
  a	
  harmful	
  effect	
  on	
  humans	
  if	
  they	
  drink	
  them	
  and	
  can	
  also	
  
cause	
  eutrophication.	
  
Eutrophication	
  
• In	
  most	
  lakes	
  and	
  rivers	
  there	
  is	
  naturally	
  very	
  little	
  nitrate	
  and	
  so	
  this	
  is	
  the	
  limiting	
  factor	
  for	
  plant	
  
and	
  algal	
  growth	
  
• Ass	
  the	
  amount	
  of	
  nitrate	
  increases	
  due	
  to	
  leaching,	
  plants	
  and	
  algae	
  grow	
  massively	
  
• As	
  algae	
  mostly	
  grow	
  on	
  the	
  surface	
  massive	
  algal	
  blooms	
  form	
  and	
  this	
  absorbs	
  the	
  light	
  and	
  stops	
  
it	
  reaching	
  the	
  lower	
  depths	
  
• Light	
  can’t	
  reach	
  the	
  plants	
  at	
  the	
  bottom	
  so	
  these	
  die	
  
• The	
  increase	
  in	
  dead	
  plant	
  matter	
  causes	
  decomposers	
  to	
  grow	
  
• These	
  are	
  aerobic	
  so	
  require	
  a	
  large	
  amount	
  of	
  oxygen	
  from	
  the	
  water	
  
• This	
  massively	
  increases	
  the	
  BOD	
  (biochemical	
  oxygen	
  demand)	
  
• Oxygen	
  then	
  becomes	
  the	
  limiting	
  factor	
  for	
  aerobic	
  organisms	
  such	
  as	
  fish	
  
• If	
  these	
  can’t	
  swim	
  away	
  (e.g.	
  they	
  are	
  in	
  a	
  pond)	
  they	
  will	
  die	
  
• There	
  is	
  less	
  competition	
  for	
  anaerobic	
  organisms	
  whose	
  populations	
  rise	
  massively	
  
• These	
  organisms	
  further	
  digest	
  the	
  plant	
  waste	
  and	
  this	
  leads	
  to	
  more	
  nitrates	
  in	
  the	
  water	
  and	
  also	
  
toxic	
  substances	
  such	
  as	
  hydrogen	
  sulphide	
  
	
  

	
  
Biology	
  Unit	
  4	
  –	
  Section	
  3.4.7	
  
During	
  succession	
  a	
  number	
  of	
  common	
  things	
  happen:	
  
•
•
•
•
•

The	
  non-­‐living	
  (abiotic)	
  environment	
  becomes	
  less	
  hostile	
  –	
  soil	
  forms,	
  nutrients	
  are	
  more	
  
plentiful	
  and	
  plants	
  provide	
  shelter	
  
Greater	
  number	
  and	
  variety	
  of	
  habitats	
  
Increased	
  biodiversity	
  –	
  as	
  different	
  species	
  occupy	
  the	
  habitats	
  
More	
  complex	
  food	
  webs	
  
Increased	
  biomass	
  –	
  especially	
  during	
  the	
  mid-­‐stages	
  

	
  
(©	
  nelson	
  thornes	
  AQA	
  Biology	
  A	
  text	
  book)	
  
	
  

Managing	
  succession	
  
•
•
•
•
•
•

To	
  reach	
  the	
  climax	
  community	
  the	
  land	
  has	
  gone	
  through	
  a	
  lot	
  of	
  stages	
  
Most	
  of	
  the	
  species	
  present	
  in	
  the	
  earlier	
  stages	
  are	
  no	
  longer	
  present	
  
However	
  to	
  get	
  grass	
  to	
  allow	
  cattle	
  to	
  graze	
  we	
  need	
  grass,	
  this	
  is	
  at	
  one	
  of	
  the	
  earlier	
  stages	
  of	
  
succession	
  
To	
  do	
  this	
  we	
  manage	
  succession	
  by	
  not	
  allowing	
  it	
  to	
  proceed	
  past	
  this	
  point	
  
This	
  can	
  be	
  achieved	
  by	
  mowing	
  or	
  allowing	
  cattle	
  to	
  eat	
  the	
  grass	
  
If	
  this	
  was	
  simply	
  left	
  shrubs	
  would	
  develop	
  and	
  then	
  trees	
  such	
  as	
  oaks	
  to	
  make	
  deciduous	
  
woodland	
  

This	
  is	
  one	
  example	
  but	
  there	
  are	
  many.	
  Succession	
  is	
  managed	
  to	
  allow	
  the	
  plants	
  to	
  grow	
  that	
  we	
  want/	
  
are	
  useful	
  rather	
  than	
  letting	
  everything	
  develop	
  to	
  the	
  climax	
  community.	
  
	
  
Unit 4 revision notes
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Unit 4 revision notes

  • 1.   Biology  A2   Unit  4  Revision  Notes     AQA    
  • 2. Biology  Unit  4  –  3.4.1   Definitions   Word   Ecosystem   Definition   An  ecosystem  is  made  up  of  all  the  interacting  biotic   (living)  and  abiotic  (non-­‐living)  factors  in  a  specific   area     Population   Is  a  group  of  interbreeding  organisms  of  one  species   in  a  habitat   Community   All  the  populations  of  different  organisms  in  a  given   area  at  a  given  time   Habitat   Place  where  a  community  of  organisms  lives  (e.g.   decaying  log)   Ecological  Niche   How  an  organism  fits  into  the  environment,  refers   to  both  where  an  organism  lives  and  what  it  does   there.  No  two  species  occupy  the  same  niche     Investigating  populations   Quadrats   3  factors  to  consider:   1. The  size  of  quadrat  to  use  –  this  will  depend  on  the  size  of  whatever  you’re  sampling  and  how  they   are  distributed  within  the  sample  area   2. The  number  of  quadrats  to  record  within  the  sample  area  –  for  questions  asking  about  this  10  or   more  should  be  used  within  each  area  to  get  a  big  enough  sample  size,  basically  more  is  better   3. The  position  of  each  quadrat  within  the  sample  area  –  random  sampling  should  be  used   Random  sampling   • This  is  used  to  prevent  bias  –  as  someone  might  pick  to  place  a  quadrat  where  there  is  a  large   amount  of  clover  for  example  but  this  may  not  be  representative.   You  should:   • • • Place  two  tape  measures  at  right  angles  along  two  sides  of  the  area  you’re  studying   Obtain  co-­‐ordinates  using  a  random  numbers  table   Place  quadrats  at  the  intersection  of  each  pair  of  co-­‐ordinates   Systematic  sampling  using  transects   • Transect  is  a  line  or  tape   • This  can  be  used  more  effectively  than  quadrats  for  measuring  things  such  as  abundance  of  species   as  you  enter  a  forest  or  measuring  the  abundance  of  species  comared  with  how  far  away  from  the   sea  you  are  
  • 3. They  can  be  used  in  two  ways:   Any  organism  over  which  the  line  passes  is  recorded   You  can  put  a  quadrat  down  every  however  many  meters  down  the  transect   • • Measuring  abundance   Sampling  is  used  to  get  a  measure  of  abundance.  This  is  the  number  of  individuals  of  a  species  within  a   given  space.  Two  ways  of  measuring  this:   • • Frequency  –  counting  individual  animals  or  plants   Percentage  cover  –  an  estimate  of  the  area  within  the  quadrat  that  one  species  covers   Mark-­‐release-­‐recapture   This  is  carried  out  to  determine  the  population  size.  This  method  is  used:   • • • • A  known  number  of  animals  are  caught   These  are  marked  in  some  way   These  are  then  released  back  into  the  community   Later  more  animals  are  caught  and  the  number  of  marked  individuals  are  recorded   Then  this  formula  is  used  to  determine  population  size  (needs  to  be  remembered  for  exam):   𝑒𝑠𝑡𝑖𝑚𝑎𝑡𝑒𝑑  𝑝𝑜𝑝𝑢𝑙𝑎𝑡𝑖𝑜𝑛  𝑠𝑖𝑧𝑒 𝑛𝑢𝑚𝑏𝑒𝑟  𝑜𝑓  𝑖𝑛𝑑𝑖𝑣𝑖𝑑𝑢𝑎𝑙𝑠  𝑖𝑛  𝑠𝑎𝑚𝑝𝑙𝑒  1  ×  𝑛𝑢𝑚𝑏𝑒𝑟  𝑜𝑓  𝑖𝑛𝑑𝑖𝑣𝑖𝑑𝑢𝑎𝑙𝑠  𝑖𝑛  𝑠𝑎𝑚𝑝𝑙𝑒  2 =     𝑛𝑢𝑚𝑏𝑒𝑟  𝑜𝑓  𝑚𝑎𝑟𝑘𝑒𝑑  𝑖𝑛𝑑𝑖𝑣𝑖𝑑𝑢𝑎𝑙𝑠  𝑖𝑛  𝑠𝑎𝑚𝑝𝑙𝑒  2 This  technique  relies  on  a  number  of  assumptions:   • • • • • The  marked  individuals  distribute  themselves  evenly  amongst  the  other  individuals  in  the   population.   No  deaths  or  births  in  the  population   No  emigration  or  immigration  effecting  the  population   The  method  of  marking  is  non-­‐toxic  to  the  individual  and  it  doesn’t  make  them  more  liable  to   predation   The  mark  isn’t  rubbed  off  or  lost  during  the  investigation  
  • 4. Population  growth  curves     1. Lag  phase  –  slow  growth  –  small  numbers  initially  so  reproduction  slow  –  organisms  are  getting   used  to  the  conditions   2. Log  phase  –  rapid  growth  and  optimum  conditions   3. Stationary  phase  –  stable  state  –  no  population  growth  –  small  fluctuations  due  to  changes  in   factors  such  as  food  supply     Abiotic  factors   • • • • Temperature  –  each  species  has  an  optimum  –  the  further  away  from  this  you  go  the  smaller  the   population  that  can  be  supported   Light  –  ultimate  source  of  energy  for  ecosystems  –  rate  of  photosynthesis  increases  as  light   intensity  increases  –  this  allows  a  larger  primary  consumer  population  to  be  supported   pH  –  this  effects  the  action  of  enzymes  –  a  population  of  organisms  is  larger  where  the  optimum  pH   is   water  and  humidity  –  where  water  is  scarce  populations  are  small  and  only  well  adapted  organisms   survive  –  humidity  effects  the  later  loss  from  plants  (transpiration)  and  animals  in  dry  air  conditions   only  those  individuals  with  adaptations  to  this  will  survive   Competition   Intraspecific   • • • • Individuals  of  the  same  species   Competing  for  resources  such  as  food,  space,  light  etc.   Availability  of  the  resources  that  determines  population  size   Lower  the  availability  smaller  the  population  size  and  vice  versa   Interspecific   • • • Individuals  of  different  species   Competing  for  resources  such  as  food,  space,  light  etc.   Competitive  advantage  determines  which  population  will  grow  
  • 5. • If  conditions  remain  the  same  this  will  lead  to  the  complete  removal  of  one  species  as  they  can’t   compete  in  this  niche   Predation   • Occurs  when  one  organism  is  consumed  by  another   Data  can  be  inaccurate  on  this  as  it  has  to  be  measured  in  the  wild  by  sampling  which  is  only  as  good  as  the   methods  used.  None  of  these  methods  guarantee  complete  accuracy  so  caution  is  advised  with  any  data   produced  this  way   Predator-­‐prey  relationship     • • • • • • Predators  eat  their  prey  therefore  reducing  the  population  size  of  the  prey   With  fewer  prey  available  the  predators  are  in  greater  competition  with  each  other  for  the   remaining  prey   The  predator  population  is  reduced  as  some  individuals  are  unable  to  consume  enough  prey  to   survive  and  reproduce   With  fewer  predators  left  less  prey  is  consumed   The  prey  population  increases   More  prey  are  now  available  so  predator  population  increases   This  is  a  cycle  and  carries  on  and  as  the  graph  shows   The  human  population   Factors  effecting  population  size   • • • • Birth  rate   Death  rate   Immigration   Emigration   𝑝𝑜𝑝𝑢𝑙𝑎𝑡𝑖𝑜𝑛  𝑔𝑟𝑜𝑤𝑡ℎ = 𝑏𝑖𝑟𝑡ℎ𝑠 + 𝑖𝑚𝑚𝑖𝑔𝑟𝑎𝑡𝑖𝑜𝑛 − (𝑑𝑒𝑎𝑡ℎ𝑠 + 𝑒𝑚𝑖𝑔𝑟𝑎𝑡𝑖𝑜𝑛)  
  • 6. 𝑝𝑒𝑟𝑐𝑒𝑛𝑡𝑎𝑔𝑒  𝑝𝑜𝑝𝑢𝑙𝑎𝑡𝑖𝑜𝑛  𝑔𝑟𝑜𝑤𝑡ℎ =   𝑝𝑜𝑝𝑢𝑙𝑎𝑡𝑖𝑜𝑛  𝑐ℎ𝑎𝑛𝑔𝑒  𝑑𝑢𝑟𝑖𝑛𝑔  𝑡ℎ𝑒  𝑝𝑒𝑟𝑖𝑜𝑑  ×100%   𝑝𝑜𝑝𝑢𝑙𝑎𝑡𝑖𝑜𝑛  𝑎𝑡  𝑡ℎ𝑒  𝑠𝑡𝑎𝑟𝑡  𝑜𝑓  𝑡ℎ𝑒  𝑝𝑒𝑟𝑖𝑜𝑑 Factors  effecting  birth  rates   • • • • • Economic  conditions   Cultural  and  religious  backgrounds   Social  pressures  and  conditions   Birth  control   Political  factors   𝐵𝑖𝑟𝑡ℎ  𝑟𝑎𝑡𝑒 =   𝑡𝑜𝑡𝑎𝑙  𝑛𝑢𝑚𝑏𝑒𝑟  𝑜𝑓  𝑏𝑖𝑟𝑡ℎ𝑠  𝑝𝑒𝑟  𝑦𝑒𝑎𝑟 ×1000   𝑡𝑜𝑡𝑎𝑙  𝑝𝑜𝑝𝑢𝑙𝑎𝑡𝑖𝑜𝑛  𝑖𝑛  𝑡ℎ𝑒  𝑠𝑎𝑚𝑒  𝑦𝑒𝑎𝑟 Factors  effecting  death  rate   • • • • • • • Age   Life  expectancy   Food  supply   Availability  of  safe  drinking  water  and  effective  sanitation   Access  to  medical  care   Natural  disasters   War   𝐷𝑒𝑎𝑡ℎ  𝑟𝑎𝑡𝑒 =   𝑡𝑜𝑡𝑎𝑙  𝑛𝑢𝑚𝑏𝑒𝑟  𝑜𝑓  𝑑𝑒𝑎𝑡ℎ𝑠  𝑝𝑒𝑟  𝑦𝑒𝑎𝑟 ×1000   𝑡𝑜𝑡𝑎𝑙  𝑝𝑜𝑝𝑢𝑙𝑎𝑡𝑖𝑜𝑛  𝑖𝑛  𝑡ℎ𝑒  𝑠𝑎𝑚𝑒  𝑦𝑒𝑎𝑟 Demographic  Transition     (©  Nelson  Thornes  A2  AQA  Biology  text  book)   This  summarises  a  pattern  that  different  countries  have  gone  through  as  they  have  developed  
  • 7. Age  population  pyramids   These  fit  into  the  demographic  transition  stages  based  on  the  shape  of  the  pyramid:   Stage   1   Pyramid   Shape  explanation   Bigger  bars  at  the  bottom   and  less  at  the  top  as  high   birth  rate  so  lots  of   children  but  also  high   death  rate  so  less  older   people     2   Still  high  birth  rate  so  big   bars  at  the  bottom  but   decreasing  death  rate  so   more  in  the  middle  and   more  people  living  longer.                       3     Low  death  rate  so  bigger   bars  in  the  middle  and   more  people  reaching  the   top  of  the  pyramid.  Birth   rate  is  decreasing  so  less   at  the  bottom  of  the   pyramid    
  • 8. 4   Low  birth  rate  and  death   rate  so  smaller  bars  at  the   bottom  and  bigger  bars  in   the  middle  also  more   people  reaching  the  top  so   larger  bars  there            
  • 9. Biology  Unit  4  –  Section  3.4.2   Why  do  organisms  need  energy   • • • • • • Metabolism  –  all  the  reactions  that  take  place  in  living  organisms  involve  energy   Movement   Active  transport   Maintenance,  repair  and  division  of  cells   Production  of  enzymes  and  hormones   Maintenance  of  body  temperature     Hydrolysis  (ATP  +  Water)     ATP   ADP  +  Pi       Energy  from  respiration       Synthesis  of  ATP  from  ADP   This  requires  the  addition  of  a  phosphate  to  ADP.  There  are  3  ways  this  occurs:   • • • PHOTOPHOSOHORYLATION  –  This  occurs  in  chlorophyll  containing  plant  cells  during  photosynthesis   OXIDATIVE  PHOSPHORYLATION  –  Which  occurs  in  the  mitochondria  of  plant  and  animal  cells  during   the  process  of  the  electron  transport  chain   SUBSTRATE  –  LEVEL  PHOSPHORYLATION  -­‐    Occurs  in  plant  and  animal  cells  when  donor  molecules   donate  phosphate  to  the  ADP  to  make  ATP  like  in  the  formation  of  pyruvate   ATP  IS  AN  IMMEDIATE  ENERGY  SOURCE  AND  ISN’T  A  GOOD  LONG  TERM  STORE  OF  ENERGY.  IT  RELEASES  ENERGY  IN   MANAGABLE  Q UANTITIES   F OR   C ELL   R EACTIONS   A ND   C AN   Q UICKLY   B E   R EFORMED   M AKING   I T   A   G OOD   IMMEDIATE   ENERGY  SOURCE      
  • 10. Biology  Unit  4  –  Section  3.4.3   Overview  of  photosynthesis   1. Capturing  of  light  energy  by  chloroplast  pigments   2. Light  dependent  reaction   3. Light  independent  reaction   Structure  of  a  chloroplast     (image  from  passscience.blogspot.com)   • • Light  dependent  reaction  takes  place  in  the  thylakoids   Light  independent  reaction  takes  place  in  the  stroma   Light  dependent  reaction   Two  purposes:   • • Add  phosphate  to  ADP  making  ATP  (photophosphorylation)   Split  water  into  H+  ions,  electrons  and  Oxygen  (photolysis)   Oxidation  and  reduction   Oxidation  –  The  loss  of  electrons,  the  loss  of  hydrogen  or  the  gain  of  oxygen   Reduction  –  The  gain  of  electrons,  the  gain  of  hydrogen  or  the  loss  of  oxygen   Wordy  Explanation  of  what  happens   • When  chlorophyll  molecules  absorb  light  energy  a  pair  it  boosts  the  energy  of  a  a  pair  of  electrons   which  raises  them  to  a  higher  energy  level   • These  electrons  have  so  much  energy  that  they  leave  the  chlorophyll  molecule   • They  are  taken  up  by  an  electron  carrier   • The  pair  of  electrons  are  now  passed  along  a  series  of  electron  carriers  in  oxidation  reduction   reactions  (located  in  the  membrane  of  the  thylakoids)   • Each  carrier  is  at  a  slightly  lower  energy  level  than  the  last  and  the  electrons  lose  energy   • This  energy  is  used  to  add  a  phosphate  to  ADP  to  make  ATP   • The  photolysis  of  water  also  happens   • This  is  due  to  chlorophyll  molecules  losing  two  electrons  so  these  need  to  be  replaced  
  • 11. • These  replacement  electrons  are  provided  by  the  splitting  of  water  molecules  as  shown  below:   2𝐻! 𝑂 → 4𝐻! + 4𝑒 ! + 𝑂!   • The  H+  is  taken  up  by  NADP  to  form  reduced  NADP  (or  NADPH2)  this  then  enters  the  light   independent  reaction   Diagrammatic  explanation     (©  Nelson  Thornes  AQA  A2  Biology  Text  Book)   Light  independent  reaction   • • Products  of  light  dependent  reaction  (reduced  NADP  and  ATP)  are  used   Takes  place  in  the  stroma  of  the  chloroplasts   Wordy  explanation   1. Carbon  dioxide  diffuses  from  the  air  eventually  into  the  stroma  of  the  chloroplast   2. Here  it  combines  with  the  5  carbon  Ribulose  Bisphosphate  (RuBP)   3. This  produces  two  molecules  of  glycerate-­‐3-­‐phosphate  (GP)   4. ATP  and  reduced  NADP  from  the  light  dependent  reaction  are  then  used  to  reduce  GP  into  triose   phosphate  (TP)   5. NADP  (non-­‐reduced)  is  reformed  and  goes  back  into  the  light  dependent  reaction  to  accept  more   hydrogen   6. Some  triose  phosphate  molecules  are  used  to  make  other  useful  substances  such  as  glucose   7. Most  triose  phosphate  molecules  are  used  to  regenerate  RuBP  using  ATP  
  • 12. Diagram     (©  Nelson  Thornes  AQA  A2  Biology  Text  Book)   Limiting  factors  on  photosynthesis   At  any  given  moment  the  rate  is  limited  by  the  factor  that  is  at  its  least  favourable  value     Factors  that  limit  photosynthesis   • • • Light  intensity   Temperature     CO2  Concentration   Temperature  is  a  factor  as  it’s  an  enzyme  controlled  reaction  so  higher  temperature  gives  the  molecules   more  energy  and  therefore  they  collide  with  the  right  amount  of  energy  with  the  enzyme  and  form  more   enzyme  substrate  complexes.        
  • 13. Biology  Unit  4  –  Section  3.4.4   Aerobic  respiration   4  stages:   • • • • Glycolysis   Link  reaction   Krebs  Cycle   Electron  Transport  Chain   Glycolysis   • Phosphorylation  of  Glucose   • Splitting  of  phosphorylated  glucose   • Oxidation  of  triose  phosphate  (by  removal  of  hydrogen)   • Hydrogen  accepted  by  NAD  to  form  NADH2   • Production  of  ATP   • Formation  of  pyruvate      
  • 14. Link  Reaction   • Pyruvate  oxidised  by  removal  of  hydrogen   • Hydrogen  accepted  by  NAD  to  form  NADH2   • De-­‐carboxylation  occurs  producing  carbon  dioxide   • Acetyl  group  formed  (2-­‐carbon)   • Combines  with  coenzyme  A  to  form  the  2  carbon  acetylcoenzyme  A     Krebs  Cycle   • Acetylcoenzyme  A  feeds  into  this  from  the  link  reaction   • Acetylcoenzyme  A  combines  with  a  4  carbon  compound  to  form  a  6  carbon  compound   • This  then  undergoes  2  decarboxylation’s  removing  two  molecules  of  carbon  dioxide   • It  also  reduces  two  hydrogen  carriers:  NAD  and  FAD  forming  reduced  NAD  and  reduced  FAD   • This  then  is  the  4  carbon  compound  needed  to  combine  with  another  acetylcoenzyme  A  molecule   and  so  it  keeps  on  going    
  • 15.   Electron  transport  chain   • Hydrogen  atoms  collected  by  coenzymes  NAD  and  FAD  are  used   • These  are  split  into  electrons  and  protons   • The  electrons  get  passed  down  the  electron  transport  chain  where  as  they  are  passed  from  carrier   to  carrier  they  lose  energy   • This  energy  is  used  to  combine  a  phosphate  with  ADP  to  make  ATP   • The  protons  are  pumped  into  the  inter-­‐membrane  space  of  the  mitochondria  (the  space  between   the  cristae  and  the  outer  membrane   • As  they  accumulate  here  they  diffuse  back  through  special  channels     • At  the  end  of  the  chain  the  electrons  combine  with  these  protons  and  oxygen  to  form  water       Anaerobic  Respiration   • • • • • When  there  is  little  or  no  oxygen  neither  the  krebs  cycle  or  electron  transport  chain  can  take  place   Only  glycolysis  can   So  lots  of  pyruvate  produced  in  order  to  produce  the  2  ATP  molecules  glycolysis  yields  (net  yield)   NAD  must  be  regenerated  so  pyruvate  accepts  this  hydrogen   However  this  happens  differently  in  animals  and  plants   In  plants  and  some  microorganisms     𝑝𝑦𝑟𝑢𝑣𝑎𝑡𝑒 + 𝑟𝑒𝑑𝑢𝑐𝑒𝑑  𝑁𝐴𝐷   → 𝑒𝑡ℎ𝑎𝑛𝑜𝑙 + 𝑐𝑎𝑟𝑏𝑜𝑛  𝑑𝑖𝑜𝑥𝑖𝑑𝑒 + 𝑁𝐴𝐷  
  • 16.     In  animals   𝑝𝑦𝑟𝑢𝑣𝑎𝑡𝑒 + 𝑟𝑒𝑑𝑢𝑐𝑒𝑑  𝑁𝐴𝐷   → 𝑙𝑎𝑐𝑡𝑎𝑡𝑒   𝑙𝑎𝑐𝑡𝑖𝑐  𝑎𝑐𝑖𝑑 + 𝑁𝐴𝐷   In  both  cases  this  is  very  inefficient  as  only  2  ATP  molecules  are  produced  by  glycolysis  and   there  is  a  much  greater  quantity  produced  by  Aerobic  respiration      
  • 17. Biology  Unit  4  –  Section  3.4.5   Food  Chains  and  Food  Webs   Producers   Photosynthetic  organisms  that  manufacture  organic  substances  using  light  energy   Consumers   Organisms  that  obtain  their  energy  by  feeding  on  other  organisms.  Those  that  directly  eat  plants  are  called   primary  consumers.  The  animals  eating  those  organisms  are  called  secondary  consumers  and  so  on.  Not   normally  more  than  4  consumers  in  a  food  chain   Decomposers   When  producers  and  consumers  die  these  organisms  make  the  energy  contained  in  the  other  organisms   available  for  the  food  chain  again  by  breaking  down  the  producer/consumer.  These  nutrients  can  then  be   absorbed  by  plants  and  used  in  the  food  chain   Food  webs   In  reality  many  animals  don’t  rely  on  a  single  food  source  and  in  a  habitat  many  food  chains  link  together   forming  a  food  web  with  lots  of  different  organisms  interacting     Energy  Losses  in  food  chains   Producers:   • Over  90%  of  the  suns  energy  reflected  back  into  space  by  clouds  and  dust  or  absorbed  by  the   atmosphere   • Not  all  wavelengths  of  light  can  be  absorbed  and  used  for  photosynthesis   • Light  may  not  fall  of  a  chlorophyll  molecule   • A  factor  such  as  low  carbon  dioxide  levels  may  limit  the  rate  of  photosynthesis   Formula  to  work  out  NP  (net  production)   𝑁𝑒𝑡  𝑝𝑟𝑜𝑑𝑢𝑐𝑡𝑖𝑜𝑛 = 𝑔𝑟𝑜𝑠𝑠  𝑝𝑟𝑜𝑑𝑢𝑐𝑡𝑖𝑜𝑛 − 𝑟𝑒𝑠𝑝𝑖𝑟𝑎𝑡𝑜𝑟𝑦  𝑙𝑜𝑠𝑠𝑒𝑠   Losses  by  primary  consumers:   • Some  of  the  organism  isn’t  eaten  
  • 18. • • • Some  parts  can’t  be  digested   Energy  lost  in  excretory  products  such  as  urine   Energy  losses  due  to  heat  loss   Why  are  food  chains  short?   • Most  food  chains  only  have  4  or  5  trophic  levels  as  there  isn’t  enough  energy  available  to  support  a   population  at  another  level   • The  total  mass  of  the  organisms  in  a  particular  place  (biomass)  is  less  at  higher  trophic  levels   • The  total  amount  of  energy  stored  is  less  at  each  level  as  you  move  up  the  food  chain    Calculating  efficiency  of  energy  transfer   𝑒𝑛𝑒𝑟𝑔𝑦  𝑡𝑟𝑎𝑛𝑠𝑓𝑒𝑟 =   𝑒𝑛𝑒𝑟𝑔𝑦  𝑎𝑣𝑎𝑖𝑙𝑎𝑏𝑙𝑒  𝑎𝑓𝑡𝑒𝑟  𝑡ℎ𝑒  𝑡𝑎𝑛𝑠𝑓𝑒𝑟 ×100%   𝑒𝑛𝑒𝑟𝑔𝑦  𝑎𝑣𝑎𝑖𝑙𝑎𝑏𝑙𝑒  𝑏𝑒𝑓𝑜𝑟𝑒  𝑡ℎ𝑒  𝑡𝑟𝑎𝑛𝑠𝑓𝑒𝑟 Ecological  pyramids   Numbers   • The  actual  numbers  of  organisms  proportional  to  each  other   • Drawbacks:   o No  account  is  taken  of  size  so  a  large  oak  tree  is  just  1  even  though  lots  of  aphids  can  feed   on  it   o This  will  create  an  inverted  pyramid  shape   o Number  of  individuals  can  be  so  great  that  it  is  impossible  to  represent  them  accurately  on   the  same  scale   Biomass   • Measure  of  the  total  mass  of  plants/animals  in  a  particular  place   • More  reliable  and  quantitative   • Only  the  organisms  present  at  a  particular  time  are  measured  so  this  doesn’t  take  into  account   seasonal  differences   Energy   • Most  accurate  representation  of  energy  flow  through  an  ecosystem   • This  measures  the  energy  stored  in  organisms   • Collecting  data  however  can  be  difficult  and  complex   • Always  a  pyramid  shape   How  productivity  is  effected  by  farming  practices   Fertilisers   Fertilisers  are  substances  farmers  spread  on  the  soil  to  replace  nutrients,  normally  nitrogen,  which  plants   get  from  the  soil.   Natural  fertilisers   Natural  fertilisers  are  the  faeces  of  animals  which  can  be  spread  onto  the  soil  to  increase  the  nutrients   available  to  the  plants.  The  advantages  are  that  this  is  free  and  readily  available  to  the  farmer,  however  the   disadvantage  is  that  it  has  to  be  left  in  the  soil  to  rot  down  so  that  the  nutrients  are  available  to  the  plants,   which  takes  time.  They  may  also  contain  pathogens  which  can  be  harmful  to  the  plant  
  • 19. Artificial  fertilisers   Artificial  fertilisers  contain  nitrogen  compounds  such  as  ammonia  which  are  spread  onto  the  soil  normally   in  soluble  granules  to  increase  nitrogen,  and  other  nutrients,  concentrations  in  the  soil.  The  advantages  are   that  the  nitrogen  is  readily  available  for  plants  to  take  up  and  use.  Farmers  can  also  buy  types  which  have   special  controlled  release  technology  which  means  there  is  a  constant  stable  nitrogen  content  going  into   the  soil.  The  disadvantages  are  the  cost,  as  the  fertiliser  is  very  expensive  whereas  natural  fertilisers  are   free.  Also  after  long  term  use  as  the  fertiliser  doesn’t  replace  the  trace  mineral  content  in  the  soil,  these   can  run  out  and  aren’t  present  in  the  crop  or  the  fruit  so  humans  or  animals  aren’t  consuming  these  trace   elements  which  can  be  bad  for  health.   Fertilisers  add  nutrients  to  the  soil  to  help  increase  the  productivity  of  plants   Pesticides   There  are  two  types,  chemical  and  biological,  both  do  the  job  of  killing  pests  which  feed  on  food  crops  and   fruit  which  ultimately  can  mean  that  farmers  don’t  get  the  maximum  yield  from  their  crops.   A  pesticide  should  be:   • • • • Specific  –  only  target  the  plant/insect/fungus  it  is  supposed  to  not  the  crop  or  any  other  organisms   Biodegrade  –  so  once  it  has  been  used  it  doesn’t  go  into  the  soil  and  kill  the  crop,  however  it  also   needs  to  have  a  long  shelf  life   Cost-­‐effective  –  developing  a  pesticide  costs  a  lot  and  new  pesticides  only  are  effective  for  a  short   length  of  time.   Not  accumulate  –  so  it  doesn’t  build  up  in  the  food  chain  and  cause  problems  for  other  organisms   Biological  control   This  is  using  the  predator  of  the  pest  to  control  the  numbers  of  the  pest.  Its  advantages  are:   • • • Very  specific   Once  introduced  the  predator  breeds  so  keeps  numbers  up  so  has  a  long  term  effect   Pests  can’t  become  resistant   The  ideal  situation  is  for  the  predator  to  exist  in  balance  with  the  pest  keeping  the  pest  at  a  level  where  it   has  no  or  little  effect.     There  are  however  some  disadvantages  with  this  method  these  are:   • • They  do  not  act  as  quickly,  as  the  predator  has  to  build  up  its  numbers  so  there  is  a  lag  between   introducing  the  predator  and  seeing  a  significant  drop  in    the  pest  numbers   The  predator  may  become  a  pest  for  example  if  there  are  few  natural  predators  to  it  or  as  the  pest   population  decreases  it  may  use  the  crops  as  a  food  source.   Integrated  systems   Integrated  control  involves:   • • • Choosing  animal  or  plant  varieties  which  are  as  pest  resistant  as  possible   Managing  the  environment  to  provide  habitats  for  natural  pest  controlling  organisms  to  live   Regularly  checking  crops  for  signs  of  pest  activity  
  • 20. • • • Removing  pest  mechanically  (hands,  vacuum,  making  barriers)   Using  biological  agents   Only  using  chemical  pesticides  as  a  last  resort   All  pests  damage  or  compete  with  plants  or  animals  leading  to  reduced  productivity   Intensive  rearing  of  livestock   Intensive  farming  is  about  converting  the  smallest  possible  amount  of  food  energy  into  the  largest  possible   amount  of  animal  biomass.  This  is  achieved  by  minimise  the  energy  lost  by  animals  during  their  lifetime.   Ways  in  which  this  is  achieved:   • • • • • •   Movement  is  restricted  –  less  energy  used  in  muscle  contraction   Keeping  the  environment  warm  (for  warm  blooded  animals)  –  reduced  heat  loss  from  body   Feeding  controlled  –  animals  receive  the  optimum  amount  and  type  of  food  for  maximum  growth   Predators  are  excluded  –  no  loss  to  other  organisms   Selective  breeding  –  produces  animals  which  are  the  most  efficient  at  converting  the  food  they  eat   into  biomass   Using  hormones  to  increase  growth  rates    
  • 21. Biology  Unit  4  –  Section  3.4.6   See  pages  at  the  end  of  the  carbon  and  nitrogen  cycle  diagrams,  as  these  are  the  first  bit  of   this  section.  Also  see  section  3.4.5  first  as  there  is  some  overlap  with  the  use  of  fertilisers   Effects  of  nitrogen  fertilisers   Reduced  species  diversity   This  is  because  nitrogen  rich  soils  favour  the  growth  of  fast  growing  species,  these  out  compete  many   other  species  which  causes  these  other  species  to  die  as  a  result   Leaching   This  is  a  process  where  nutrients  are  removed  from  the  soil.  Rain  water  will  dissolve  any  soluble  nutrients   and  carry  them  deep  into  the  soil  eventually  beyond  the  reach  of  the  plant  roots.  These  eventually  find   their  way  into  water  courses.  They  can  have  a  harmful  effect  on  humans  if  they  drink  them  and  can  also   cause  eutrophication.   Eutrophication   • In  most  lakes  and  rivers  there  is  naturally  very  little  nitrate  and  so  this  is  the  limiting  factor  for  plant   and  algal  growth   • Ass  the  amount  of  nitrate  increases  due  to  leaching,  plants  and  algae  grow  massively   • As  algae  mostly  grow  on  the  surface  massive  algal  blooms  form  and  this  absorbs  the  light  and  stops   it  reaching  the  lower  depths   • Light  can’t  reach  the  plants  at  the  bottom  so  these  die   • The  increase  in  dead  plant  matter  causes  decomposers  to  grow   • These  are  aerobic  so  require  a  large  amount  of  oxygen  from  the  water   • This  massively  increases  the  BOD  (biochemical  oxygen  demand)   • Oxygen  then  becomes  the  limiting  factor  for  aerobic  organisms  such  as  fish   • If  these  can’t  swim  away  (e.g.  they  are  in  a  pond)  they  will  die   • There  is  less  competition  for  anaerobic  organisms  whose  populations  rise  massively   • These  organisms  further  digest  the  plant  waste  and  this  leads  to  more  nitrates  in  the  water  and  also   toxic  substances  such  as  hydrogen  sulphide      
  • 22. Biology  Unit  4  –  Section  3.4.7   During  succession  a  number  of  common  things  happen:   • • • • • The  non-­‐living  (abiotic)  environment  becomes  less  hostile  –  soil  forms,  nutrients  are  more   plentiful  and  plants  provide  shelter   Greater  number  and  variety  of  habitats   Increased  biodiversity  –  as  different  species  occupy  the  habitats   More  complex  food  webs   Increased  biomass  –  especially  during  the  mid-­‐stages     (©  nelson  thornes  AQA  Biology  A  text  book)     Managing  succession   • • • • • • To  reach  the  climax  community  the  land  has  gone  through  a  lot  of  stages   Most  of  the  species  present  in  the  earlier  stages  are  no  longer  present   However  to  get  grass  to  allow  cattle  to  graze  we  need  grass,  this  is  at  one  of  the  earlier  stages  of   succession   To  do  this  we  manage  succession  by  not  allowing  it  to  proceed  past  this  point   This  can  be  achieved  by  mowing  or  allowing  cattle  to  eat  the  grass   If  this  was  simply  left  shrubs  would  develop  and  then  trees  such  as  oaks  to  make  deciduous   woodland   This  is  one  example  but  there  are  many.  Succession  is  managed  to  allow  the  plants  to  grow  that  we  want/   are  useful  rather  than  letting  everything  develop  to  the  climax  community.