2. •What is Protein Targeting?
•Why protein is targeted?
•Where it occurs?
3.
4. signal sequences
• First by Gunter Blobel (1970) ,won the Nobel Prize (1999) for his
work on protein targeting.
• Directs a protein to its appropriate location after synthesis.
• Signals vary from 13 to 36 amino acid residues.
a. About 10 to 15 hydrophobic amino acid residues.
b. One or more positively charged residues near N-terminus.
c. A short cleavage sequence often including(polar A.A) Ala.
13. Summary• Protein targeting is necessary for proteins that are destined
to work outside the cytoplasm.
• Requires sorting signals that direct their transport from the
cytosol.
• Correct sorting is crucial for the cell; errors can lead to
diseases.
• Protein targeting is more complex in eukaryotes because of
the presence of many intracellular compartments.
• targeting either of two ways depending on the protein ….
1) Co translational translocation .
2) Posttranslational translocation .
14. References
• Wikipedia… www.google.co.in.
• LEHNINGER :- principles of Biochemistry ; Cox and Nelson ;
5th edition ; page no.= 1100-1107.
• Molecular cell Biology:- Lodish and Berk et al ; 5th edition ;
page no.= 657-739.