1. BHANDARI SEJAL RAJESH
LODHA DEEPALI
NARENDRA
FORMULATION AND EVALUATION OF HERBAL
OINTMENT OF LANTANA CAMARA FOR
ASSESSMENT OF ANTIMICROBIAL ACTIVITY
The Presentation on
2. INDEX
Aim and Objective
Introduction
Materials and Methods
Formulation
Evaluation Parameters
Conclusion
References
3. INTRODUCTION:
Plant Profile:
• Lantana camara (common lantana) is a species of flowering plant belonging to Family
Verbenaceae.
• The stems and branches are generally thorny due to presence of trichomes. The leaves are
organized in opposite pairs. Flowers are Present in cluster form.
• Vernacular Names: Lantana, Tantani, Ghaneri
Taxonomical Classification:
Kingdom : Plantae
Subkingdom :Viridiplantae
Division : Tracheophyta
Sub-division : Spermatophytina
Family : Verbenaceae
Genus : Lantana
Species : L. camara
4. AIM AND OBJECTIVE
Aim: To study the medicinal Uses of Lantana camara and to formulate Herbal Ointment.
Objective:
1.To Search the medicinal plant with therapeutic Importance and Prepare the
suitable Formulation.
2.To Evaluate the Antimicrobial Activity of the Formulation.
3.To Perform Evaluation the test for Prepared Formulation.
5. MATERIAL AND METHODS:
Extraction Procedure:
• The collected Plant material was dedusted by cloth, and the contaminating material and
unwanted plant material were removed.
• Further material was washed thoroughly with water. The washed material was
kept for 3 days for shade drying.
• After complete drying the material was pulverized in the Mortar Pestle and the
material size required for extraction was achieved.
• The 50 gm of crude form of lantana was taken in 500ml RBF and the Solvent
mixture selected for the extraction procedure is added to the RBF. The solvent
mixture constitutes Methanol and Water in 70:30 proportions.
• The solvent was added to RBF in the manner that all crude material was
sufficiently soaked in a solvent system.
• The system is kept aside for 2 days for soaking and extraction. Afterwards, the
extraction assembly was arranged as per procedure.
• The condenser was attached to the RBF and water circulation started. The
material was heated for about 2-3 Hrs At 50 degrees.
• The residue obtained was filtered and the filtrate was evaporated to get the
semisolid extract.
6. FORMULATION
Ointment Base:
• The Pre-weighed Hard Paraffin was added to the Evaporating Dish kept on the Water
bath and was allowed to dissolved.
• Accurately weighed Cetosteryl alcohol and wool fat was incorporated in evaporating
dish and stirred by stirrer until it Dissolve completely in Hard Paraffin.
• Lastly the weighed Yellow Soft Paraffin was added to the Evaporating dish.
• Formula Table:
Sr.
No
.
Ingredients Quantity
1. Wool Fat (Lanolin) 0.5 gm
2. Hard Paraffin 0.5 gm
3. Cetosteryl Alcohol 0.5 gm
4. Yellow Soft Paraffin 8.5 gm
Table 1.1:Formula Table For Ointment Base
7. Ointment:
• The Prepared Ointment Base was Common for All Three Formulations with varying
Concentrations. The Trituration method was utilized for the Preparation of the ointment.
• The weighed ointment base was taken into the Mortar pastle.
• The Extract was weighed accordingly and incorporated into the ointment base with
constant trituration.
• Lastly the liquid Paraffin was added and the mixture was triturated constantly until
getting the uniform mass of ointment.
Sr.
No
Ingredients Formulation
A
Formulation
B
Formulation
C
1. Hydroalcoholic
extract
1.5 gm 2.5 gm 3.0 gm
2. Liquid Paraffin 1 gm 1 gm 1 gm
3. Ointment Base 7.5 gm 6.5 gm 6 gm
Table 1.2:Formula Table for Ointment.
8. EVALUATION PARAMETERS:
1. Organoleptic Properties:
The Physical Parameters are visually examined.
2. pH:
The skin pH plays the critical role in Absorption of the formulation. The pH of
the formulation was measured by using the pH Paper. The Triplicate values
are recorded and the Average was taken. The pH of Formulation was found to
be 6.5.
Sr.No
.
Test Inference
1. Colour Light Green
2. Odour Natural
3. Texture Homogenous
Table 1.3: Organoleptic Evaluation
Sr.No
.
Test Observation Conclusion
1. pH Light Green
colour
Slightly
Acidic pH.
(6-7)
Table 1.4: pH of Formulation
9. 3. Spreadability:
• The spreadability was one of the important evaluation parameters for the topical
preparations with regard to patient comfort. An adequate value for the Spreadability
confirms the even distribution of the formulation.
• Procedure: The excess sample is placed between two glass slides. The slides were then
compressed to an even thickness by placing a definite weight for a definite time. The
time taken to separate the two slides was measured. Spreadability is inversely
proportional to the Time taken for separation.
10. 4. Washability:
• The formulation was applied on the skin then the ease of washing with water is checked.
The formulation is applied to the skin and kept for 30 Min.
• The Greasy Appearance persisted with water and was wiped fully with the Alcohol.
• The Solubility Study is also done simultaneously with the Ointment base and was found
to be Water Insoluble and Alcohol Soluble.
Sr.No
.
Test Observation Evaluation
1. Washability Greasy
Appearance
Persisted with
water and
washed out
with alcohol
Water
Insoluble
2. Solubility of
Base
Water
Insoluble
Alcohol
Soluble
Alcohol
Soluble
Table1.5: Washability Evaluation
11. 5. Non-Irritancy Test:
• This is the important evaluation parameter of topical preparations. Also called as the
Skin Patch test..
• Every topical Preparation is applied on the skin of a human being and observed for the
effect or any kind of inflammation for a particular period of time.
• The Bases used in Ointment may cause any kind of allergic reactions or inflammation.
• There were no signs of Irritancy after 30 minutes of Application.
Sr.No
.
Test Observation Evaluation
1 Non-Irritancy Test No Allergic
or any kind
of
Inflammation
is Observed.
Passed
Table1.6: Irritancy Test
12. 6. Consistency :
• Consistency must be in optimum value so that it gives formulation a good
texture and at the same time it must be uniformly distributed topically. Smooth
and no greediness observed.
Sr.N
o
Test Observation Evaluation
1. Consistency
(Homogeniety)
Stable
Uniform
Texture.
Passed
Table1.7: Evaluation of Consistency
13. CONCLUSION:
The Present study was to prepare and evaluate herbal ointment. For this the
herbal extract of Lantana camara L was prepared by hydrodistillation method to
obtain higher yield of extract without loss of active constituents. The Trituration
method was used to prepare ointment so that even mixing of the herbal extract with
the ointment base was occurred which was stable during the storage. The Evaluation
parameters were studied which shows confirmatory results.
On the basis of this investigation, it is concluded that plant Lantana camara L
showed significant activities against several topical itching disorders and the
formulation can be used against the Skin Pruritus and the Infectious disorders.
The formulation also executes the Antimicrobial Activity.
14. REFERENCES
Rahmatullah M, Jahan R, Azam FM, Hossan S, Mollik MA, Rahman T. Folk medicinal
uses of Verbenaceae family plants in Bangladesh. Afr J Tradit Complement Altern Med
2011;8:53-65.
Tadesse E, Engidawork E, Nedi T, Mengistu G. Evaluation of the anti-diarrheal activity
of the aqueous stem extract of Lantana camara Linn (Verbenaceae) in mice. BMC
Complement Altern Med 2017;17:190.
Patel S. A weed with multiple utilities: Lantana camara. Rev Environ Sci Biotechnol
2011;10:341-51.
Wangrawa DW, Badolo A, Guelbéogo WM, Nébié R, Sagnon N. Insecticidal properties
of local plants used against Anopheles gambiae, a malaria vector in Burkina Faso, West
Africa. Am J Trop Med Hyg 2014;915:35.
Zhao Y, Yang G, Ren D, Zhang X, Yin Q, Sun X. Luteolin suppresses growth and
migration of human lung cancer cells. Mol Biol Rep 2011;38:1115-9. Begum S, Zehra
SQ, Siddiqui BS, Fayyaz S, Ramzan M. Pentacyclic triterpenoids from the aerial parts of
Lantana camara and their nematicidal activity. Chem Biodivers 2008;5:1856-66.
