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Spectroscopic Characterization of Nanoparticles for
Potential Drug Discovery
C. Mark Talbott, Ph.D.
Shimadzu Scientific Instruments, Columbia, MD., USA
2 / 41	
Gold Nanoparticles
Nanoscience/nanotechnology has emerged as one of the
fastest growing fields of science this decade.1
Various nanoparticles, including carbon nanotubes,
quantum dots, and gold nanoparticles, have been
extensively studied for biomedical applications.
Of particular interest are the improvement in efficacy and
reduction in toxicity that have been demonstrated with
these nanoparticle-based drug delivery systems.2
3 / 41	
Gold Nanoparticles
The inertness and biocompatibility of gold nanoparticles
(Au-NPs) make them very promising for specific
applications such as medical imaging, drug delivery,
gene delivery, and molecular sensing.3
Large gold crystal 400X Hoffman modulation
4 / 41	
In each of these applications, the particle size and shape
of Au-NPs are critical.
The distinct absorption peak from the surface plasmon
absorption of the gold nanoparticles is located between
510 and 530 nm.
The surface plasmon is a collective excitation of electrons
at the interface between the conductor and insulator.4
Absorption of light by the surface plasmon of Au-NPs
accounts for the colorful appearance of these solutions,
which in turn is a direct characteristic of their size and
aggregation.
Gold Nanoparticles
5 / 41	
Gold nanoparticles ranging in size from 15 to 100 nm are
readily available and can be easily synthesized.
In a simple preparation scheme, gold nanoparticles can be
stabilized by the citrate ion and further functionalized by
a wide array of compounds. This scheme allows for the
easy preparation of chemistries between the Au-NPs
and other compounds of interest.
Gold Nanoparticles
6 / 41	
In this study, Au-NPs were prepared from the citrate
reduction of AuCl3, and Au-NP size and refractive index
were evaluated using UV-Vis and laser diffraction particle
size instrumentation.
The Au-NPs were then mixed with various organic
compounds, and the complexes formed were further
evaluated spectroscopically.
The remainder of this presentation will be a report
of the results of these evaluations.
Gold Nanoparticles
7 / 41	
Experimental
Preparation of gold nanoparticles
Gold nanoparticles were synthesized and stabilized
by the citrate reduction of a 0.001 molar chloroauric
acid solution.
Newly synthesized gold nanoparticles
demonstrating a deep crimson color
8 / 41	
Preparation of gold nanoparticles
Gold nanoparticles were synthesized using various
molar ratios of citrate in order to investigate particle
size control in the synthesis.
Completion of the reduction reaction could be readily
evaluated by polarized optical microscopy.
Experimental
9 / 41	
Preparation of gold nanoparticles
Particle size of the synthesized Au-NPs was evaluated
by monitoring the Au-NP visible absorption band using
a Shimadzu UV-2600 UV-Vis spectrophotometer and a
2-mm quartz cell.
Synthesized Au-NP (after agglomeration)
800X Hoffman modulation
Experimental
10 / 41	
Preparation of gold nanoparticles
The particle size was confirmed by laser diffraction
particle size analysis using a Shimadzu SALD-7101
particle size analyzer.
The refractive index of the Au-NPs was estimated by
the Becke line method of optical microscopy and
confirmed by the SALD-7101 particle size software.
Shimadzu SALD-7101 laser
diffraction particle size analyzer
Experimental
11 / 41	
Preparation of gold nanoparticle organic complexes
Gold nanoparticle organic complexes were prepared
by mixing the citrate-stabilized Au-NPs with various
organic compounds of interest, followed by vortexing.
To assure Au-NP size uniformity, freshly prepared Au-NPs
were used.
Organic compounds used were lysozyme, casein,
prednisone, protein, phosphatidyl choline, and Igg.
Experimental
12 / 41	
Spectral Analysis
UV-Vis spectra of the neat organic solutions and Au-NP
complexes were acquired as transmission spectra through
a quartz cuvette with a 2-mm path length.
Dilution of the complex mixtures was not necessary.
2-mm path length quartz cuvette
Experimental
13 / 41	
Spectral Analysis
FTIR transmission spectra of the organic neat and complex
Au-NP solutions were acquired by placing 30 µL of the
Au-NP solution on a section of IR-transparent silicon wafer.
The silicon wafer was placed on the surface of a warm
hot plate (approximately 130 degrees Celsius) to
allow evaporation.
They were then placed in the FTIR and scanned
either by transmission or diffuse reflectance.
Experimental
14 / 41	
Results/Discussion
Size Analysis – Spectroscopy
Gold NP visible absorption directly related to nanoparticle size 6,7
15 / 41	
Size Analysis – Spectroscopy
Addition of citrate to the chloroauric acid solution in molar ratios of 0.17 to 1.4
acts to stabilize various nanoparticle sizes. The Au-NP absorption band moves
from 577 nm to 523 nm, suggesting an initial Au-NP size of 100 nm and a final
Au-NP size of 20 nm at the 1.4:1 citrate to gold molar ratio.
Results/Discussion
16 / 41	
All further studies were focused on the 1.4:1 citrate to gold molar
ratio with a target Au-NP size of approximately 20 nm.
Size Analysis – Spectroscopy
Results/Discussion
17 / 41	
Particles size measurements using a Shimadzu SALD-7101 provided Au-NP
particles size measurement of 15 nm (+/- 23) and suggested a refractive
index of 1.70. The refractive index of the agglomerated Au-NPs via the
Becke line method also suggest an RI of between 1.648 and 1.709.
Becke line into the Au-NP with
RI of 1.648 and into RI solution
with RI of 1.709
RI = 1.648 RI = 1.709
Refractive Index Analysis – Spectroscopy
Results/Discussion
18 / 41	
UV-Vis Analysis of Au-NP Complexes – Conjugate Formation
UV-Vis spectra of the conjugated Au-NP complexes show the change in the
Au-NP absorption as different complexes are formed.
The big question: Did a conjugated complex form?
UV-Vis spectra for Au-NP
conjugated with organics to
form Au-NP complexes:
Cyan Igg
Magenta Phosphatidyl choline
Blue Protein
Red Au-NP
Grey Casein
Green Prednisone
Results/Discussion
19 / 41	
UV-Vis Analysis of Au-NP Complexes – Verification of Crystal Formation
Further confirmation of conjugation was the change in crystal form of the final
conjugated complex.
Au-NP 400X Pol-tint Casein 400X Pol-tint Casein-Au-NP 400X Pol
Igg 400X Pol-tint Igg-Au-NP 400X Pol
Results/Discussion
20 / 41	
UV-Vis Analysis of Au-NP Complexes – Verification of Crystal Formation
Again, confirmation of conjugation was the change in crystal form of the final
conjugated complex.
Au-NP 400X Pol-tint Lysozyme 400X Pol-tint Lysozyme-Au-NP 400X Pol-tint
Phosphatidyl choline 400X Phosphatidyl choline-Au-Np
Pol-tint 400X Pol-tint
Results/Discussion
21 / 41	
UV-Vis Analysis of Au-NP Complexes – Verification of Crystal Formation
In all complex formations, the significant changes in crystal form and anisotropy
strongly support the formation of a Au-NP conjugate complex.
Au-NP 400X Pol-tint Prednisone 400X HF-PACO Prednisone-AuNP 400X Pol-tint
Protein 400X HF-PACO Protein 400X POL-tint
Results/Discussion
22 / 41	
UV-Vis Analysis of Au-NP Complexes – Optimization
The microscopic crystal study suggests that the differences observed in the
UV-Vis spectra for the conjugate complex formations are real and may
provide a method to easily monitor and characterize complex chemistry.
UV-Vis spectra for Au-NP
conjugated with organics to
form Au-NP complexes:
Cyan Igg
Magenta Phosphatidyl choline
Blue Protein
Red Au-NP
Grey Casein
Green Prednisone
Results/Discussion
23 / 41	
UV-Vis Analysis of Au-NP Complexes – Optimization
In this quick study, Au-NP formed conjugated complexes were not optimized
for any given complex. Optimization would consist of adjusting the Au-NP
size and buffer (pH) for the desired task at hand. UV-Vis analysis is again
suggested as an excellent method for monitoring and optimizing Au-NP
and Au-NP conjugated complexes.
UV-Vis spectra for Au-NP
conjugated with organics to
form Au-NP complexes:
Cyan Igg
Magenta Phosphatidyl choline
Blue Protein
Red Au-NP
Grey Casein
Green Prednisone
Results/Discussion
24 / 41	
UV-Vis Analysis of Au-NP Complexes – Stability
UV-Vis spectra of Au-NP complexes immediately after synthesis and several
days after formation, showing the stability of the conjugated complex.
Blue/cyan
Protein complex
Green/light green
Igg complex
Magenta/pink
Phosphatidyl choline
complex
Results/Discussion
25 / 41	
FTIR Analysis of Au-NP Complexes
FTIR spectra of the gold nanoparticles (black) and sodium citrate (red).
The Au-NP spectrum shows four distinct absorption bands at 1734, 1599, 1448,
and 1245 cm-1.
Results/Discussion
26 / 41	
FTIR Analysis of Au-NP Complexes
The four characteristic absorption bands of the Au-NP can be readily seen in
all of the conjugated complexes. The Au-NP absorption bands either show as
distinct absorption bands in the conjugated complex or can be observed in the
shoulders of other complex characteristic absorption bands.
4
Red Au-NP
Light green Igg
Orange Protein
Dark green Prednisone
Magenta Phosphatidyl Choline
Black Casein
Blue Lysozyme
321
Results/Discussion
27 / 41	
The characteristic Au-NP absorption bands do not shift with conjugation.
This suggests that the conjugate formation does not involve significant
changes to the molecular bonding of the Au-NPs.
4321
Red Au-NP
Light green Igg
Orange Protein
Dark green Prednisone
Magenta Phosphatidyl Choline
Black Casein
Blue Lysozyme
FTIR Analysis of Au-NP Complexes
Results/Discussion
28 / 41	
FTIR spectra of the protein, lysozyme, and Igg conjugated complexes show
the characteristic amide I and amide II bands at 1665 and 1534 cm-1.
Amide I
Amide II
Red Au-NP
Light green Igg
Orange Protein
Dark green Prednisone
Magenta Phosphatidyl Choline
Black Casein
Blue Lysozyme
FTIR Analysis of Au-NP Complexes
Results/Discussion
29 / 41	
Protein and Protein-Au-NP (red)Igg and Igg-Au-NP (red)
Lysozyme and Lysozyme-Au-NP (red)
These amide absorption bands do not
show shifts from their non-conjugated
spectra, suggesting that the amide
moieties (C=O and N-H) do not take
part in formation of the Au-NPs’
conjugated complexes.
FTIR Analysis of Au-NP Complexes
Results/Discussion
30 / 41	
Protein and Protein-Au-NP (red)Igg and Igg-Au-NP (red)
Lysozyme and Lysozyme-Au-NP (red)
This also suggests that any
inter- or intra-molecular H-bonding that
these functionalities may be involved
in is not affected by complexation
with the Au-NPs.
FTIR Analysis of Au-NP Complexes
Results/Discussion
31 / 41	
Protein and Protein-Au-NP (red)Igg and Igg-Au-NP (red)
Lysozyme and Lysozyme-Au-NP (red)
No significant band shape or wavenumber
shift is observed in the OH absorptions of
these compounds either before or after
Au-NP complex formation, suggesting that
the OH moieties do not play a role in
conjugate complex formation. H-bonding
does not appear to be a part of the complex
formation.
FTIR Analysis of Au-NP Complexes
Results/Discussion
32 / 41	
Phosphatidyl choline & PC-Au-NP (red) Prednisone and Pred-Au-NP (red)
The phosphatidyl choline and prednisone spectra do show significant changes in
the OH absorption bands before and after Au-NP complex formation, suggesting
that the OH functionalities and/or H-bonding associated with the OH
functionalities of those molecules may either be disrupted with complex
formation or may take an active part in the complex formation itself. Shifts and
changes in the C=O absorption band also suggest possible H-bonding changes
with complex formation.
FTIR Analysis of Au-NP Complexes
Results/Discussion
33 / 41	
FTIR spectra of all conjugated complexes show a unique absorption band between
1428 and 1314 cm-1 that is not found in the non-conjugated organic spectra.
This absorption band is composed of two underlying peaks at 1401 cm-1 and
1362 cm-1.
1362 cm-1
1401 cm-1
FTIR Analysis of Au-NP Complexes
Red Au-NP
Light green Igg
Orange Protein
Dark green Prednisone
Magenta Phosphatidyl Choline
Black Casein
Blue Lysozyme
Results/Discussion
34 / 41	
Was the citrate replaced?
The absence of several of the citrate absorption bands in the spectra of the
complexes further suggests that the citrate was replaced in the organic complexes.
Citrate (red)
Results/Discussion
35 / 41	
Conclusion
Importance of Spectroscopic Techniques
in Au-NP Research
The slides presented here have clearly shown the power
and advantages of using spectroscopic techniques
such as UV-Vis and FTIR to monitor and characterize
gold nanoparticles and gold nanoparticle conjugated systems.
UV-visible spectra of conjugated complexes gave
information on nanoparticle size and also offered a quick
method to aid in the optimization of particle size and
buffer pH in conjugate complex formation.
36 / 41	
Importance of Spectroscopic Techniques
in Au-NP Research
Acquired FTIR spectra readily showed the formation of the
conjugate complexes and further provide information
about bonding and structural characteristics of such systems.
Conclusion
37 / 41	
Importance of Spectroscopic Techniques
in Au-NP Research
Modern UV-Vis spectrophotometers, such as those used in
this study, offer wider spectral ranges up to 1400 nm and
lower stray light values, allowing for photometric ranges easily
extending to 8 Abs. These modern benches provide the
nanoparticle researcher opportunities to study conjugated
systems without the need for dilution.
In addition, modern spectrophotometers reaching into the NIR
provide the possibility of monitoring conjugated phenomenon
such as a NIR-trigger mechanism for conjugate release.
Conclusion
38 / 41	
Importance of Spectroscopic Techniques
in Au-NP Research
Newer FTIR systems easily offer rapid scanning with high
sensitivity, resolution, and signal-to-noise ratios providing
for the possibilities of monitoring nanoparticle reaction
kinetics.
Conclusion
39 / 41	
Investigations into nanoparticles have already impacted
advancements in current manufacturing, electronics,
photovoltaics, ophthalmics, and medicine, to name just a
few. Further advances are on the near horizon.
Modern analytical instrumentation will continue to
advance to provide the best tools for the researcher’s needs
and be the support structure that helps bring these
advances to fruition.
Conclusion
40 / 41	
Acknowledgements
This work was conducted at Shimadzu Scientific
Instruments in Columbia, Maryland, as part of an ongoing
effort to provide state-of-the-art scientific instrumentation that
readily meets the needs of current researchers.
This author acknowledges the efforts of the Shimadzu
staff in support of this goal.
Acknowledgements also go to Dr. Suja Sukuraman, Andrew
Shaff, and Dr. Robert Clifford.
41 / 41	
References
(1) Chen, PC; Gold Nanoparticles; Nanotechnology, Science an
Applications 2008, 1, 45.
(2)  Cheng, Z.; Zaki, Ajlna; Tsourkas, A.; Multifunctional Nanoparticles:
Cost Versus Benefit of Adding Targeting and Imaging Capabilities,
Science, 2012, 338, pp. 903-910.
(3) De Jong, WH; Form, PJ,; Int. J. Nanomedicine 2008, 3(2), 133.
(4) Rayford, CE,; Nanoscape 2005, 2(3), 33.
(5) McFarland, CL; Haynes, CA; Mirkin, RP; Van Duyne, Godwin, HA,;
J. Chem. Educ. 2004, 81, 544A.
(6)  Sukumaran, S; Talbott, CM,; Drug Discover & Development 2011,
http://www.dddmag.com/articles/2011/12/spectroscopic-view-
nanoparticles-biomedical-applications.
(7) Sigma Aldrich, http://www.sigmaaldrich.com/materials-science/
nanomaterials/gold-nanoparticles.html, retrieved November 13, 2012.
42 / 41	
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research, products or services, please visit our support
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Spectroscopic Characterization of Nanoparticles for Potential Drug Discovery

  • 1. 1 / 41 Spectroscopic Characterization of Nanoparticles for Potential Drug Discovery C. Mark Talbott, Ph.D. Shimadzu Scientific Instruments, Columbia, MD., USA
  • 2. 2 / 41 Gold Nanoparticles Nanoscience/nanotechnology has emerged as one of the fastest growing fields of science this decade.1 Various nanoparticles, including carbon nanotubes, quantum dots, and gold nanoparticles, have been extensively studied for biomedical applications. Of particular interest are the improvement in efficacy and reduction in toxicity that have been demonstrated with these nanoparticle-based drug delivery systems.2
  • 3. 3 / 41 Gold Nanoparticles The inertness and biocompatibility of gold nanoparticles (Au-NPs) make them very promising for specific applications such as medical imaging, drug delivery, gene delivery, and molecular sensing.3 Large gold crystal 400X Hoffman modulation
  • 4. 4 / 41 In each of these applications, the particle size and shape of Au-NPs are critical. The distinct absorption peak from the surface plasmon absorption of the gold nanoparticles is located between 510 and 530 nm. The surface plasmon is a collective excitation of electrons at the interface between the conductor and insulator.4 Absorption of light by the surface plasmon of Au-NPs accounts for the colorful appearance of these solutions, which in turn is a direct characteristic of their size and aggregation. Gold Nanoparticles
  • 5. 5 / 41 Gold nanoparticles ranging in size from 15 to 100 nm are readily available and can be easily synthesized. In a simple preparation scheme, gold nanoparticles can be stabilized by the citrate ion and further functionalized by a wide array of compounds. This scheme allows for the easy preparation of chemistries between the Au-NPs and other compounds of interest. Gold Nanoparticles
  • 6. 6 / 41 In this study, Au-NPs were prepared from the citrate reduction of AuCl3, and Au-NP size and refractive index were evaluated using UV-Vis and laser diffraction particle size instrumentation. The Au-NPs were then mixed with various organic compounds, and the complexes formed were further evaluated spectroscopically. The remainder of this presentation will be a report of the results of these evaluations. Gold Nanoparticles
  • 7. 7 / 41 Experimental Preparation of gold nanoparticles Gold nanoparticles were synthesized and stabilized by the citrate reduction of a 0.001 molar chloroauric acid solution. Newly synthesized gold nanoparticles demonstrating a deep crimson color
  • 8. 8 / 41 Preparation of gold nanoparticles Gold nanoparticles were synthesized using various molar ratios of citrate in order to investigate particle size control in the synthesis. Completion of the reduction reaction could be readily evaluated by polarized optical microscopy. Experimental
  • 9. 9 / 41 Preparation of gold nanoparticles Particle size of the synthesized Au-NPs was evaluated by monitoring the Au-NP visible absorption band using a Shimadzu UV-2600 UV-Vis spectrophotometer and a 2-mm quartz cell. Synthesized Au-NP (after agglomeration) 800X Hoffman modulation Experimental
  • 10. 10 / 41 Preparation of gold nanoparticles The particle size was confirmed by laser diffraction particle size analysis using a Shimadzu SALD-7101 particle size analyzer. The refractive index of the Au-NPs was estimated by the Becke line method of optical microscopy and confirmed by the SALD-7101 particle size software. Shimadzu SALD-7101 laser diffraction particle size analyzer Experimental
  • 11. 11 / 41 Preparation of gold nanoparticle organic complexes Gold nanoparticle organic complexes were prepared by mixing the citrate-stabilized Au-NPs with various organic compounds of interest, followed by vortexing. To assure Au-NP size uniformity, freshly prepared Au-NPs were used. Organic compounds used were lysozyme, casein, prednisone, protein, phosphatidyl choline, and Igg. Experimental
  • 12. 12 / 41 Spectral Analysis UV-Vis spectra of the neat organic solutions and Au-NP complexes were acquired as transmission spectra through a quartz cuvette with a 2-mm path length. Dilution of the complex mixtures was not necessary. 2-mm path length quartz cuvette Experimental
  • 13. 13 / 41 Spectral Analysis FTIR transmission spectra of the organic neat and complex Au-NP solutions were acquired by placing 30 µL of the Au-NP solution on a section of IR-transparent silicon wafer. The silicon wafer was placed on the surface of a warm hot plate (approximately 130 degrees Celsius) to allow evaporation. They were then placed in the FTIR and scanned either by transmission or diffuse reflectance. Experimental
  • 14. 14 / 41 Results/Discussion Size Analysis – Spectroscopy Gold NP visible absorption directly related to nanoparticle size 6,7
  • 15. 15 / 41 Size Analysis – Spectroscopy Addition of citrate to the chloroauric acid solution in molar ratios of 0.17 to 1.4 acts to stabilize various nanoparticle sizes. The Au-NP absorption band moves from 577 nm to 523 nm, suggesting an initial Au-NP size of 100 nm and a final Au-NP size of 20 nm at the 1.4:1 citrate to gold molar ratio. Results/Discussion
  • 16. 16 / 41 All further studies were focused on the 1.4:1 citrate to gold molar ratio with a target Au-NP size of approximately 20 nm. Size Analysis – Spectroscopy Results/Discussion
  • 17. 17 / 41 Particles size measurements using a Shimadzu SALD-7101 provided Au-NP particles size measurement of 15 nm (+/- 23) and suggested a refractive index of 1.70. The refractive index of the agglomerated Au-NPs via the Becke line method also suggest an RI of between 1.648 and 1.709. Becke line into the Au-NP with RI of 1.648 and into RI solution with RI of 1.709 RI = 1.648 RI = 1.709 Refractive Index Analysis – Spectroscopy Results/Discussion
  • 18. 18 / 41 UV-Vis Analysis of Au-NP Complexes – Conjugate Formation UV-Vis spectra of the conjugated Au-NP complexes show the change in the Au-NP absorption as different complexes are formed. The big question: Did a conjugated complex form? UV-Vis spectra for Au-NP conjugated with organics to form Au-NP complexes: Cyan Igg Magenta Phosphatidyl choline Blue Protein Red Au-NP Grey Casein Green Prednisone Results/Discussion
  • 19. 19 / 41 UV-Vis Analysis of Au-NP Complexes – Verification of Crystal Formation Further confirmation of conjugation was the change in crystal form of the final conjugated complex. Au-NP 400X Pol-tint Casein 400X Pol-tint Casein-Au-NP 400X Pol Igg 400X Pol-tint Igg-Au-NP 400X Pol Results/Discussion
  • 20. 20 / 41 UV-Vis Analysis of Au-NP Complexes – Verification of Crystal Formation Again, confirmation of conjugation was the change in crystal form of the final conjugated complex. Au-NP 400X Pol-tint Lysozyme 400X Pol-tint Lysozyme-Au-NP 400X Pol-tint Phosphatidyl choline 400X Phosphatidyl choline-Au-Np Pol-tint 400X Pol-tint Results/Discussion
  • 21. 21 / 41 UV-Vis Analysis of Au-NP Complexes – Verification of Crystal Formation In all complex formations, the significant changes in crystal form and anisotropy strongly support the formation of a Au-NP conjugate complex. Au-NP 400X Pol-tint Prednisone 400X HF-PACO Prednisone-AuNP 400X Pol-tint Protein 400X HF-PACO Protein 400X POL-tint Results/Discussion
  • 22. 22 / 41 UV-Vis Analysis of Au-NP Complexes – Optimization The microscopic crystal study suggests that the differences observed in the UV-Vis spectra for the conjugate complex formations are real and may provide a method to easily monitor and characterize complex chemistry. UV-Vis spectra for Au-NP conjugated with organics to form Au-NP complexes: Cyan Igg Magenta Phosphatidyl choline Blue Protein Red Au-NP Grey Casein Green Prednisone Results/Discussion
  • 23. 23 / 41 UV-Vis Analysis of Au-NP Complexes – Optimization In this quick study, Au-NP formed conjugated complexes were not optimized for any given complex. Optimization would consist of adjusting the Au-NP size and buffer (pH) for the desired task at hand. UV-Vis analysis is again suggested as an excellent method for monitoring and optimizing Au-NP and Au-NP conjugated complexes. UV-Vis spectra for Au-NP conjugated with organics to form Au-NP complexes: Cyan Igg Magenta Phosphatidyl choline Blue Protein Red Au-NP Grey Casein Green Prednisone Results/Discussion
  • 24. 24 / 41 UV-Vis Analysis of Au-NP Complexes – Stability UV-Vis spectra of Au-NP complexes immediately after synthesis and several days after formation, showing the stability of the conjugated complex. Blue/cyan Protein complex Green/light green Igg complex Magenta/pink Phosphatidyl choline complex Results/Discussion
  • 25. 25 / 41 FTIR Analysis of Au-NP Complexes FTIR spectra of the gold nanoparticles (black) and sodium citrate (red). The Au-NP spectrum shows four distinct absorption bands at 1734, 1599, 1448, and 1245 cm-1. Results/Discussion
  • 26. 26 / 41 FTIR Analysis of Au-NP Complexes The four characteristic absorption bands of the Au-NP can be readily seen in all of the conjugated complexes. The Au-NP absorption bands either show as distinct absorption bands in the conjugated complex or can be observed in the shoulders of other complex characteristic absorption bands. 4 Red Au-NP Light green Igg Orange Protein Dark green Prednisone Magenta Phosphatidyl Choline Black Casein Blue Lysozyme 321 Results/Discussion
  • 27. 27 / 41 The characteristic Au-NP absorption bands do not shift with conjugation. This suggests that the conjugate formation does not involve significant changes to the molecular bonding of the Au-NPs. 4321 Red Au-NP Light green Igg Orange Protein Dark green Prednisone Magenta Phosphatidyl Choline Black Casein Blue Lysozyme FTIR Analysis of Au-NP Complexes Results/Discussion
  • 28. 28 / 41 FTIR spectra of the protein, lysozyme, and Igg conjugated complexes show the characteristic amide I and amide II bands at 1665 and 1534 cm-1. Amide I Amide II Red Au-NP Light green Igg Orange Protein Dark green Prednisone Magenta Phosphatidyl Choline Black Casein Blue Lysozyme FTIR Analysis of Au-NP Complexes Results/Discussion
  • 29. 29 / 41 Protein and Protein-Au-NP (red)Igg and Igg-Au-NP (red) Lysozyme and Lysozyme-Au-NP (red) These amide absorption bands do not show shifts from their non-conjugated spectra, suggesting that the amide moieties (C=O and N-H) do not take part in formation of the Au-NPs’ conjugated complexes. FTIR Analysis of Au-NP Complexes Results/Discussion
  • 30. 30 / 41 Protein and Protein-Au-NP (red)Igg and Igg-Au-NP (red) Lysozyme and Lysozyme-Au-NP (red) This also suggests that any inter- or intra-molecular H-bonding that these functionalities may be involved in is not affected by complexation with the Au-NPs. FTIR Analysis of Au-NP Complexes Results/Discussion
  • 31. 31 / 41 Protein and Protein-Au-NP (red)Igg and Igg-Au-NP (red) Lysozyme and Lysozyme-Au-NP (red) No significant band shape or wavenumber shift is observed in the OH absorptions of these compounds either before or after Au-NP complex formation, suggesting that the OH moieties do not play a role in conjugate complex formation. H-bonding does not appear to be a part of the complex formation. FTIR Analysis of Au-NP Complexes Results/Discussion
  • 32. 32 / 41 Phosphatidyl choline & PC-Au-NP (red) Prednisone and Pred-Au-NP (red) The phosphatidyl choline and prednisone spectra do show significant changes in the OH absorption bands before and after Au-NP complex formation, suggesting that the OH functionalities and/or H-bonding associated with the OH functionalities of those molecules may either be disrupted with complex formation or may take an active part in the complex formation itself. Shifts and changes in the C=O absorption band also suggest possible H-bonding changes with complex formation. FTIR Analysis of Au-NP Complexes Results/Discussion
  • 33. 33 / 41 FTIR spectra of all conjugated complexes show a unique absorption band between 1428 and 1314 cm-1 that is not found in the non-conjugated organic spectra. This absorption band is composed of two underlying peaks at 1401 cm-1 and 1362 cm-1. 1362 cm-1 1401 cm-1 FTIR Analysis of Au-NP Complexes Red Au-NP Light green Igg Orange Protein Dark green Prednisone Magenta Phosphatidyl Choline Black Casein Blue Lysozyme Results/Discussion
  • 34. 34 / 41 Was the citrate replaced? The absence of several of the citrate absorption bands in the spectra of the complexes further suggests that the citrate was replaced in the organic complexes. Citrate (red) Results/Discussion
  • 35. 35 / 41 Conclusion Importance of Spectroscopic Techniques in Au-NP Research The slides presented here have clearly shown the power and advantages of using spectroscopic techniques such as UV-Vis and FTIR to monitor and characterize gold nanoparticles and gold nanoparticle conjugated systems. UV-visible spectra of conjugated complexes gave information on nanoparticle size and also offered a quick method to aid in the optimization of particle size and buffer pH in conjugate complex formation.
  • 36. 36 / 41 Importance of Spectroscopic Techniques in Au-NP Research Acquired FTIR spectra readily showed the formation of the conjugate complexes and further provide information about bonding and structural characteristics of such systems. Conclusion
  • 37. 37 / 41 Importance of Spectroscopic Techniques in Au-NP Research Modern UV-Vis spectrophotometers, such as those used in this study, offer wider spectral ranges up to 1400 nm and lower stray light values, allowing for photometric ranges easily extending to 8 Abs. These modern benches provide the nanoparticle researcher opportunities to study conjugated systems without the need for dilution. In addition, modern spectrophotometers reaching into the NIR provide the possibility of monitoring conjugated phenomenon such as a NIR-trigger mechanism for conjugate release. Conclusion
  • 38. 38 / 41 Importance of Spectroscopic Techniques in Au-NP Research Newer FTIR systems easily offer rapid scanning with high sensitivity, resolution, and signal-to-noise ratios providing for the possibilities of monitoring nanoparticle reaction kinetics. Conclusion
  • 39. 39 / 41 Investigations into nanoparticles have already impacted advancements in current manufacturing, electronics, photovoltaics, ophthalmics, and medicine, to name just a few. Further advances are on the near horizon. Modern analytical instrumentation will continue to advance to provide the best tools for the researcher’s needs and be the support structure that helps bring these advances to fruition. Conclusion
  • 40. 40 / 41 Acknowledgements This work was conducted at Shimadzu Scientific Instruments in Columbia, Maryland, as part of an ongoing effort to provide state-of-the-art scientific instrumentation that readily meets the needs of current researchers. This author acknowledges the efforts of the Shimadzu staff in support of this goal. Acknowledgements also go to Dr. Suja Sukuraman, Andrew Shaff, and Dr. Robert Clifford.
  • 41. 41 / 41 References (1) Chen, PC; Gold Nanoparticles; Nanotechnology, Science an Applications 2008, 1, 45. (2)  Cheng, Z.; Zaki, Ajlna; Tsourkas, A.; Multifunctional Nanoparticles: Cost Versus Benefit of Adding Targeting and Imaging Capabilities, Science, 2012, 338, pp. 903-910. (3) De Jong, WH; Form, PJ,; Int. J. Nanomedicine 2008, 3(2), 133. (4) Rayford, CE,; Nanoscape 2005, 2(3), 33. (5) McFarland, CL; Haynes, CA; Mirkin, RP; Van Duyne, Godwin, HA,; J. Chem. Educ. 2004, 81, 544A. (6)  Sukumaran, S; Talbott, CM,; Drug Discover & Development 2011, http://www.dddmag.com/articles/2011/12/spectroscopic-view- nanoparticles-biomedical-applications. (7) Sigma Aldrich, http://www.sigmaaldrich.com/materials-science/ nanomaterials/gold-nanoparticles.html, retrieved November 13, 2012.
  • 42. 42 / 41 Thank you for viewing this presentation. Should you have any questions or require additional information about our research, products or services, please visit our support page: www.ssi.shimadzu.com/support/ @shimadzussiFollow us on Twitter Need More Info?