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GREEN BIOTECHNOLOGY AND
BIO-NANOTECHNOLOGY
FOR ENVIRONMENT
SUSTAINABILITY,MONITORING
AND FUTURE ASPECTS
PRESENTED BY,
RISHAV RAY
M.Sc. IN ENVIRONMENTAL SCIENCE, SEMESTER-III
DEPARTMENT OF ENVIRONMENTAL STUDIES
VISVA-BHARATI,SANTINIKETAN
NANOSCIENCES
NANOSCIENCES
SCANNING
TUNNELING
MICROSCOPE
BIOTECHNOLOGY NANOTECHNOLOGY
BIO-NANOTECHNOLOGY
INFOTECH
•Pharmaceuticals and their
metabolites are also
recognized as emerging
contaminants
•Not metabolized
completely
•Excreted in the form of
original parent compound
or in form of derivative
•Discharge into
wastewaters may lead to
emergence of antibiotic-
resistant strains of
microorganisms
• More than 200 different pharmaceutical agents, including antibiotics, hormones,
NSAIDs have been detected in several aquatic as well as terrestrial environments
• Not easily removed by sewage treatment plants (STPs) or WWTPs
• They can remain in the aqueous phase for longer times because of their hydrophilic
and stable nature.
• These drugs along with their metabolites are detected in surface waters frequently
posing the environmental threats
DRAWBACKS
•EXPENSIVE
•TIME- CONSUMING
• EXTENDED RESPONSE TIME
•LENGTHY RECOVERY TIME
•LESS SELECTIVE
• Enzymes used for biological recognition
• conversion of antibody-antigen interactions into a detectable signal
by the transduction element
• Directly immobilized on the transducer surface by several
methods(covalent bonding, physical adsorption by van der Waals
forces, encapsulation, covalent cross-linking ,ionic bonding)
• Signal is measured as a result of variations in ionic concentration
caused due to specific immunoreactions.
• Mechanism of detection is based on the very specific interaction of
a particular drug with enzyme target followed by the inhibition of
the enzyme activity.
ENZYME AND IMMUNOSENSOR BASED BIOSENSOR
WHY THEM?
•Technology is time saving
•High sensitivity
•High specificity
•Quick response time
•Excellent thermal and electrical conductivity of metal
&carbon-based nanomaterials such as carbon
nanotubes (CNTs)
These nanomaterials have demonstrated successful removal of hazardous pollutants
from ground/surface water, soil, and sediments.
( NANOTUBES) (NANOFIBRES ) (NANOCOMPOSITES)
•Incorporation of microbial strains in nZVI helps in more
efficient remediation of pollutants.
•A reductive-oxidative strategy consisting of nZVI and
(Sphingomonas species)found to be effective for
degradation of PBDEs in aqueous solution
•Shewanella oneidensis ,immobilized in beads containing
carbon nanotubes, reduces Manganese and chromium
levels in water
SEQUENTIAL
METHODS
COMBINED
contaminant is
subjected to
nanoparticles& Bio
agent is added
Both nanoparticle
and biological agent
are added to the
system simultaneously
SCOPES OF NANO PARTICLES IN FUTURE
•BIOMEDICAL AND HEALTH CARE
•FOOD AND AGRICULTURE
•INDUSTRIAL
•ELECTRONICS
•ENVIRONMENT
•RENEWABLE ENERGY
•TEXTILES
REFERENCES
• Benelmekki M, An introduction to nanoparticles and nanotechnology. In: Designing hybrid
nanoparticles. Morgan & Claypool Publishers, San Rafael(2015)
• Bharagava RN, Saxena G, Chowdhary P,Constructed wetlands: an emerging phytotechnology for
degradation and detoxification of industrial wastewaters. In: Bharagava RN (ed) Environmental
pollutants and their bioremediation approaches, 1st edn. CRC Press,Taylor & Francis Group, Boca
Raton, pg 397–426. https://doi.org/10.1201/9781315173351-15(2017)
• Chandra R, Saxena G, Kumar V ,Phytoremediation of environmental pollutants: an eco-sustainable
green technology to environmental management. In: Chandra R (ed) Advances in biodegradation
and bioremediation of industrial waste, 1st edn. CRC Press, Taylor & Francis Group, Boca Raton, pg
1–30. https://doi.org/10.1201/b18218-2 (2015)
• Advances in protein/enzyme-based biosensors for the detection of pharmaceutical contaminants
in the environment,dhar dhruva, Roy Shounak, Department of Bio-Engineering, Birla Institute of
Technology, Mesra, Ranchi, India,BioX Centre and School of Basic Sciences, Indian Institute of
Technology Mandi, Himachal Pradesh, India(2019)
• Singh R., Behera M., Department of Environmental Science, Central University of Rajasthan, Ajmer,
Rajasthan, India, Kumar S., Centre for Environmental Sciences, Central University of Jharkhand,
Ranchi, Jharkhand, India© Springer Nature Singapore Pt Ltd. 2019 Bhargava R.N., Saxena G. (eds.),
Bioremediation of Industrial Waste for Environmental Safety, https://doi.org/10.1007/978-981-13-
3426-9_7(2019)
• Nanotechnology in India-PT’S IAS ACADEMY-(2017)

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Grand seminar on green bt and bionanotech

  • 1. GREEN BIOTECHNOLOGY AND BIO-NANOTECHNOLOGY FOR ENVIRONMENT SUSTAINABILITY,MONITORING AND FUTURE ASPECTS PRESENTED BY, RISHAV RAY M.Sc. IN ENVIRONMENTAL SCIENCE, SEMESTER-III DEPARTMENT OF ENVIRONMENTAL STUDIES VISVA-BHARATI,SANTINIKETAN
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  • 8. •Pharmaceuticals and their metabolites are also recognized as emerging contaminants •Not metabolized completely •Excreted in the form of original parent compound or in form of derivative •Discharge into wastewaters may lead to emergence of antibiotic- resistant strains of microorganisms
  • 9. • More than 200 different pharmaceutical agents, including antibiotics, hormones, NSAIDs have been detected in several aquatic as well as terrestrial environments • Not easily removed by sewage treatment plants (STPs) or WWTPs • They can remain in the aqueous phase for longer times because of their hydrophilic and stable nature. • These drugs along with their metabolites are detected in surface waters frequently posing the environmental threats
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  • 12. DRAWBACKS •EXPENSIVE •TIME- CONSUMING • EXTENDED RESPONSE TIME •LENGTHY RECOVERY TIME •LESS SELECTIVE
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  • 14. • Enzymes used for biological recognition • conversion of antibody-antigen interactions into a detectable signal by the transduction element • Directly immobilized on the transducer surface by several methods(covalent bonding, physical adsorption by van der Waals forces, encapsulation, covalent cross-linking ,ionic bonding) • Signal is measured as a result of variations in ionic concentration caused due to specific immunoreactions. • Mechanism of detection is based on the very specific interaction of a particular drug with enzyme target followed by the inhibition of the enzyme activity. ENZYME AND IMMUNOSENSOR BASED BIOSENSOR
  • 15. WHY THEM? •Technology is time saving •High sensitivity •High specificity •Quick response time •Excellent thermal and electrical conductivity of metal &carbon-based nanomaterials such as carbon nanotubes (CNTs)
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  • 20. These nanomaterials have demonstrated successful removal of hazardous pollutants from ground/surface water, soil, and sediments. ( NANOTUBES) (NANOFIBRES ) (NANOCOMPOSITES)
  • 21. •Incorporation of microbial strains in nZVI helps in more efficient remediation of pollutants. •A reductive-oxidative strategy consisting of nZVI and (Sphingomonas species)found to be effective for degradation of PBDEs in aqueous solution •Shewanella oneidensis ,immobilized in beads containing carbon nanotubes, reduces Manganese and chromium levels in water
  • 22. SEQUENTIAL METHODS COMBINED contaminant is subjected to nanoparticles& Bio agent is added Both nanoparticle and biological agent are added to the system simultaneously
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  • 25. SCOPES OF NANO PARTICLES IN FUTURE •BIOMEDICAL AND HEALTH CARE •FOOD AND AGRICULTURE •INDUSTRIAL •ELECTRONICS •ENVIRONMENT •RENEWABLE ENERGY •TEXTILES
  • 26. REFERENCES • Benelmekki M, An introduction to nanoparticles and nanotechnology. In: Designing hybrid nanoparticles. Morgan & Claypool Publishers, San Rafael(2015) • Bharagava RN, Saxena G, Chowdhary P,Constructed wetlands: an emerging phytotechnology for degradation and detoxification of industrial wastewaters. In: Bharagava RN (ed) Environmental pollutants and their bioremediation approaches, 1st edn. CRC Press,Taylor & Francis Group, Boca Raton, pg 397–426. https://doi.org/10.1201/9781315173351-15(2017) • Chandra R, Saxena G, Kumar V ,Phytoremediation of environmental pollutants: an eco-sustainable green technology to environmental management. In: Chandra R (ed) Advances in biodegradation and bioremediation of industrial waste, 1st edn. CRC Press, Taylor & Francis Group, Boca Raton, pg 1–30. https://doi.org/10.1201/b18218-2 (2015) • Advances in protein/enzyme-based biosensors for the detection of pharmaceutical contaminants in the environment,dhar dhruva, Roy Shounak, Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi, India,BioX Centre and School of Basic Sciences, Indian Institute of Technology Mandi, Himachal Pradesh, India(2019) • Singh R., Behera M., Department of Environmental Science, Central University of Rajasthan, Ajmer, Rajasthan, India, Kumar S., Centre for Environmental Sciences, Central University of Jharkhand, Ranchi, Jharkhand, India© Springer Nature Singapore Pt Ltd. 2019 Bhargava R.N., Saxena G. (eds.), Bioremediation of Industrial Waste for Environmental Safety, https://doi.org/10.1007/978-981-13- 3426-9_7(2019) • Nanotechnology in India-PT’S IAS ACADEMY-(2017)