The document summarizes the MyGo Mini real-time PCR instrument. It is the world's most compact PCR instrument with no moving parts, enabling silent operation. It provides high precision and analytical sensitivity, able to discriminate samples that differ by as little as 10% gene expression. The solid-state design makes it highly reliable with optional 5-year warranty. It has easy-to-use software for automated analysis and reporting of PCR and high-resolution melt data.

1. REAL-TIME PCR

2. The MyGo Mini is the world’s most compact real-time
PCR instrument, with no moving parts, enabling silent
operation and maximum reliability. It provides users
with great results over a broad range of applications.
Personal precision…

3. The MyGo Mini demonstrates superior intra-run and inter-run
analytical precision in DNA quantification and melting point analysis.
Users can now address biological phenomena with subtle effects on
gene expression, or pathogen levels, for example discriminating 10%
differences in transcript concentrations.
For users performing HRM-based. analysis of genetic variants, the
combination of thermal control, optical data quality and HRM data
analysis of the MyGo Mini system provide compelling functionality.
The system can discriminate all classes of SNP, including Class 4
SNPs via HRM.
PRECISION AND
PERFORMANCE
5 10 15 20 25 30 35 40 45
0
0.25
0.5
0.75
1
1.25
Intensity(RFU)
Cycle
16 replicate PCR
amplifications of a human
gene target. Input template
5ng of total human cDNA
per reaction. Average
Cq 21.4 cycles, standard
deviation 0.015 cycles.

4. Duplicate PCR
amplifications for 6
different concentrations
of human cDNA, from
5ng per reaction to 2.4ng
per reaction, in a 1.1 fold
dilution series.
70 72.5 75 77.5 80 82.5
2500
5000
7500
10000
12500
15000
-dF/dT
Temperature ( )
24 24.5 25 25.5 26 26.5 27 27.5
0.4
0.45
0.5
Cycle
Intensity(RFU)
5.0 4.5 4.1 3.7Key (ng): 3.3 3.0 2.7 2.4
16 replicate reactions,
amplified and melted. Input
template 10,000 copies
of viral DNA per reaction.
Average Tm 81.7ºC,
standard deviation 0.015ºC.

5. EASY
ANALYSIS
The MyGo Mini system comes with easy-to-use software, and robust, high
performance algorithms which provide accurate results from complex data.
Automated analysis of complex HRM data makes this powerful technique more
accessible to non-experts, and minimises operator-dependent variability in
data analysis.
Automated report generation makes life easier for service providers, and users
operating within GLP quality systems.
-1 -0.75 -0.5 -0.25 0 0.25 0.5 0.75 1
-1
-0.5
0
0.5
1
Vector 1
Vector2
16 human DNA samples
classified into 3 different
genotypes by automatic
clustering of a class 4 HRM
data set.

6. SOLID-STATE
RELIABILITY
The MyGo Mini is the world’s first completely
solid-state real-time PCR machine, with no moving parts. The MyGo Mini
has been designed to last, then made and tested to exacting standards.
The result is a silent, highly reliable instrument, with the option of a 5 year
extended warranty.
extended
warranty

7. CONNECTIVITY
The MyGo Mini software does not require a
dedicated computer, unlike some competitor
instruments. So users do not need to find the space
or money for an additional computer to run the
system, and they can run the operating system that
they are familiar with. In addition the MyGo Mini
software enables the user to control hundreds of
instruments whilst simultaneously analysing run
data on one computer. No other qPCR system
offers these benefits to the user.
MyGo Mini Connection Modes
1. Direct network connection
2. Local area network connection
3. USB drive connection

8. SPECIFICATION
Dimensions W 12cm x D 12cm x H 16cm
Weight 2.5 kg
Operating noise Silent
Electrical
Voltage 100 - 240V AC ± 10%
Frequency 47-63Hz
Power 90W
Number of reactions 16
Format 0.1ml tubes
Reaction volume 10 - 100µl (20µl recommended)
Temperature control
Method Peltier
Range 37-99°C
Speed 3°C/s heating, 1.5°C/s cooling
Run time < 60 minutes
Temperature control
Resolution 0.01°C
Uniformity 0.05°C (SD)
Accuracy ± 0.25°C
Fluorescence
Excitation 500nm (blue LED)
Detection 510 to 560nm (CMOS array)
Multiplex level Duplex
Sensitivity Single copy detection
Dynamic range 9-log
Precision 1.1 fold discrimination
Factory calibrated dyes SYBR Green I, ResoLight, FAM,
VIC, HEX, CAL540, CAL560
User selected custom dyes Yes
Supported assay formats Intercalating dyes (e.g. SYBR
Green I), Hydrolysis Probes
Connection options LAN, Direct connection to
computer (RJ45), PC-free
(USB stick)
All trademarks are the property of their respective owners. Design and specifications are subject to change without notice.

9. COMPATIBILITY
The MyGo Mini software can be installed
on Mac OS X, Windows, and Linux
operating systems. Use your choice of
computer and operating system.
The MyGo Mini is also compatible with
standard 0.1mL low-profile tubes, which
are convenient and cost-effective.
www.mygopcr.com