2. INTRODUCTION
THYROID GLAND
The thyroid gland and parathyroid glands are a group of endocrine glands.
Thyroid gland secretes 3 hormones :
Triiodothyronine (T3),
Thyroxine (T4), and
Calcitonin.
T3 and T4 are produced by thyroid follicles and have similar biological activity.
3. Calcitonin produced by interfollicular ‘C’ cells is chemically and biologically
entirely different.
Calcitonin considered along with parathormone (PTH), with which it regulate
calcium metabolism.
Thyroxine was the first hormone synthesized in the laboratory.
4. LOCATION OF THYROID GLAND
The thyroid gland and parathyroid glands, located in the base of the
neck.
These glands play a vital role in maintaining the body's homeostasis
by producing hormones that regulate the body's metabolism and free
calcium levels.
Parafollicular cells (also called C cells) are neuroendocrine cells in
the thyroid which primary function is to secrete calcitonin.
They are located adjacent to the thyroid follicles and reside in the
connective tissue.
These cells are large and have a pale stain compared with the
follicular cells or colloid.
5. LOCATION OF THYROID GLAND
The thyroid is a butterfly-shaped gland that sits low on the front of the
neck.
The thyroid gland lies below the Adam’s apple. Along the front of the
windpipe.
The thyroid has two side lobes, connected by a bridge (isthmus) in the middle.
6. CHEMISTRY AND SYNTHESIS
2 amino acid – tyrosine
Condensation
T3 & T4
T3 is 3,5,3’- triiodothyronine.
T4 is 3,5,3’,5’-tetraiodothyronine.
7. Synthesis, Storage and Release:
Thyroid hormones are synthesized and stored in thyroid follicles as a part of
thyroglobulin molecule.
Thyroglobulin molecule is a glycoprotein synthesized by thyroid cells. Its MW
660 KDa and contains 10% sugar.
T3 and T4 synthesis, storage and release involves the following processes.
1. Iodine uptake
2. Oxidation and iodination
3. Coupling
4. Storage and release
5. Peripheral conversion of T4 to T3.
9. TRANSPORT, METABOLISM AND
EXCRETION
Thyroid hormones are bound to plasma proteins only 0.03 – 0.08 % of T4 and
0.2 – 0.5% of T3.
Almost all protein bound iodine (PBI) in plasma is thyroid hormone, of which
90 – 95% is T4 and the rest T3.
Binding occur to 3 plasma proteins in the following decreasing order of
affinity for T4:
i. Thyroxine binding globulin (TBG)
ii. Thyroxine binding prealbumin (trans-thyretin)
iii. Albumin
10. TRANSPORT, METABOLISM AND
EXCRETION
The normal concentration of PBI is 4-10 dl; only 0.1 – 0.2 μg/dl of T3, rest is
T4.
During pregnancy thyroxine binding globulin is increased – PBI levels are
elevated.
Plasma t1/2 of T4 is 6-7 days and of T3 is 1-2 days.
The half- lives are shortened in hyperthyroidism due to faster metabolism
and prolonged in hypothyroidism due t slower metabolism.
11. REGULATION OF SECRETION
Secretion of hormones from the thyroid is controlled by anterior pituitary by
the elaboration of thyrotropin.
While TSH secretion itself is regulated by TRH produced in hypothalamus.
Somatostatin elaborated by hypothalamus inhibits GH, prolactin and TSH
secretion from pituitary.
12. REGULATION OF SECRETION
The negative feedback by the thyroid hormones is
exercised directly on the pituitary as well as through
hypothalamus.
The action of TRH on pituitary and that of TSH on
thyroid cells is mediated by enhanced cAMP synthesis.
High concentration of TSH also acts via IP3/DAG
which increased intracellular Ca+2 pathway in the
thyroid cells.
13. ACTIONS OF THYROID HORMONES
The actions of T3 and T4 are qualitatively similar and are nicely depicted in the
features of hypo and hyperthyroidism.
1. Growth and Development:
T3 and T4 are essential for normal growth and development of tissue
including the nervous system.
Lack of thyroid hormone during development results in short stature and
mental deficits (cretinism).
14. ACTIONS OF THYROID HORMONES
2) CVS:
T3 and T4 cause a hyperdynamic state of circulation which is partly
secondary to increased peripheral demand and partly due to direct cardiac
actions.
T3 and T4 stimulate heart by direct action on contractile elements
(increasing the myosin fraction having greater Ca+2 ATPase activity).
Myocardial O2 consumption can be markedly reduced by induction of
hypothyroidism.
15. ACTIONS OF THYROID HORMONES
3) Nervous System:
T3 and T4 have profound functional effect on CVS.
Mental retardation is the hallmark of cretinism; sluggishness and other
behavioural features are seen in myxoedema.
Hyperthyroid individuals are anxious, nervous, excitable, exhibit tremors and
hyperreflexia.
16. ACTIONS OF THYROID HORMONES
4) Skeletal Muscle:
Muscles are flabby and weak in myxoedema, while thyrotoxicosis produces
increased muscle tone, tremor, and weakness due to myopathy.
5) GIT:
Propulsive activity of gut is increased by T3/T4.
Hypothyroid patients are often constipated, while diarrhoea is common in
hyperthyroidism.
17. ACTIONS OF THYROID HORMONES
6) Kidney:
T3 and T4 do not cause diuresis in euthyroid individuals, but the rate of urine
flow is often increased when myxoedematous patients are treated with it.
7) Haemopoiesis:
Hypothyroid patients suffer from some degree of anaemia which is restored
only by T4 treatment.
Thus, T4 appears to be facilitatory to erythropoiesis.
18. ACTIONS OF THYROID HORMONES
8) Reproduction:
Thyroid has an indirect effect on reproduction.
Fertility is impaired in hypothyroidism and women suffer from
oligomenorrhoea.
Normal thyroid function is required for maintenance of pregnancy and
lactation.
19. Mechanism of Action
Both T3 and T4 penetrate cells by active transport and produce majority of
their actions by combining with a nuclear thyroid hormone receptor (TR).
TR – which belong to the steroid and retinoid superfamily of intracellular
receptors.
It bound to the ‘thyroid hormone response element’ (TRE) in the enhancer
region of the target genes along with corepressors.
This keep gene transcription suppressed.
20. Mechanism of Action
When T3 binds to the ligand – binding domain of TR, it
heterodimerizes with retinoid X receptor and undergoes a
conformation change releasing the corepressor and binding
the coactivator.
This induces gene transcription
Production of specific mRNA and a specific pattern of
protein synthesis
Various metabolic and anatomic effects.
21. Relation between T3 and T4
Thyroid secrets more T4 than T3, but in iodine deficient state this
difference reduced.
T4 is the major circulating hormone bcz it is 15 times more tightly bound to
plasma proteins.
T3 is 5 times more potent than T4 and acts faster. Peak effect of T3 comes
in 1-2 days while that of T4 takes 6-8 days.
22. Relation between T3 and T4
T3 is more avidly bound to nuclear receptor than T4 and T4 receptor
complex is unable to activate gene transcription.
About 1/3 of T4 is converted to T3 in the thyroid cells, liver and kidney by
type 1 deiodinase (D1) and released into circulation.
T3 is generated within the target cells (skeletal muscle, heart, brain,
pituitary) by another type (D2) of deiodinase.
Thus, it is concluded that T3 is the active hormone, while T4 is mainly a
transport form; function as a prohormone of T3.
24. Marketed Preparations
Triiodothyronine (Lithyronine) is not freely available in India.
It is occasionally used i.v. along with 1-thyroxine in myxoedema coma.
Clinically, 1-thyroxine is preferred for all indications over liothyronine
because of more sustained and uniform action as well as lower risk of cardiac
arrhythmias.
26. Pharmacokinetics and interactions
Oral bioavailability of 1- thyroxine is approx. 75%, but severe hypothyroidism
can reduce oral absorption,
It should be administered in empty stomach to avoid interference by food.
Sucralfate, iron, calcium and proton pump inhibitors also reduce 1- thyroxine
absorption.
CYP3A4 inducers like rifampin, phenytoin, and carbamazepine accelerate
metabolism of T4; dose of 1-thyroxine may need enhancement.
27. USES
The most important use of thyroid hormone is for replacement therapy in
deficiency states:
1. Cretinism:
It is due to failure of thyroid development or defect in hormone synthesis or
due to extreme iodine deficiency.
Treatment with thyroxine --- 8 – 12 μg/kg daily.
28. USES
2. Adult hypothyroidism (myxoedema):
One of the commonest endocrine disorders which develops as a consequence
of autoimmune thyroiditis or thyroidectomy.
Antiboides against thyroid peroxidase or thyroglobulin are responsible for
majority of cases of adult hypothyroidism.
Important drugs that can cause hypothyroidism 131I, iodides, lithium and
amiodarone.
29. USES
Start with low dose --- 50 μg of 1- thyroxine daily increase every 2-3 weeks
to an optimum of 100 -200 μg/day. (adjusted by clinical response and serum
TSH levels)
Further dose adjustments are made at 4-6 weeks intervals needed for
reaching steady state.
Increase in dose is mostly needed during pregnancy.
30. USES
3. Myxoedema coma:
It is an emergency; characterised by progressive mental deterioration due to
acute hypothyroidism.
Drug choice is 1-thyroxine 200 – 500 μg i.v. followed by 100 μg OD.
Some authorities recommend adding low dose i.v. T3 10 μg --- 8 hourly.
Oral T4 --- 500 μg loading dose followed by 100- 300 μg daily.
31. USES
4. Nontoxic goiter:
two type - endemic and sporadic
Endemic - due to iodine deficiency
dose - - - 150- 200 μg of iodine daily
5. Thyroid nodule:
Certain benign functioning nodules regress when TSH is suppressed by T4
therapy.
Non functional nodule not responsive to TSH.