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ROLE OF PLANT HORMONES
Outline of the presentations
➢ Introduction
➢ Literature review
➢ Conclusion
introduction
➢They are various organic compounds other than nutrients
produced by plants that control or regulate germination,
growth, metabolism, or other physiological activities.
➢Also called phytohormone and recently called growth
bioregulators.
Plant Hormones & Growth
Intro....
➢Plant hormones, which are active in very low concentrations, are produced in certain parts
of the plants and are usually transported to
other parts where they elicit specific biochemical, physiological, or morphological
responses. And hey are also active in tissues where they are produced.
➢ Each plant hormone evokes many different responses. Also,
the effects of different hormones overlap and may be
stimulatory or inhibitory .
➢The commonly recognized classes of plant hormones are the auxins, gibberellins, cytokinin,
abscisic acid, and ethylene.
Intro....
❖Each hormone performs its specific functions;
❖ however, nearly all of the measurable responses of plants to heredity or
environment are controlled by interaction between two or more hormones .
❖Such interactions may occur at various levels, including
a. The synthesis of hormones,
b. Hormone receptors, and second messengers,
c. Ultimate hormone action.
Intro...
Plant hormones (or plant growth regulators, or PGRs) are internally
secreted chemicals in plants that are used for regulating the plants’
growth.
According to a standard definition, plant
hormones are:
↔Signal molecules produced at specific locations, that occur in very low
concentrations, and cause altered processes in target cells at other
locations.
Characteristics
The concentration of hormones required for the plant response
is very low(10-6 to 10-5M), comparing with the requirement of
mineral and vitamin for plants.
The synthesis of plant hormones is more diffuse and not
always localized.
Classifications of plant hormones
It is accepted that there are two major classes of plant
hormones:
class action examples
PROMOTORS Cause faster
growth
Auxins
Cytokinin(cks)
Gibberellins (gas)
Brassinosteroids
INHIBITORS Reduce growth Ethylene
Abscisic acid (ABA)
What do hormones control in plants?
➢Roots and shoots growth
➢Seed germination
➢ Leaf fall
➢Disease resistance, and water stress
➢Fruit formation and ripening
➢Flowering time
➢ Bud formation
➢Anything related to plant growth!
Plant hormones
plant hormones cont...
Auxins
Aux.....
✓Auxin is a general name for a group of hormones that are
involved with growth responses (i.e., elongate cells, stimulate
cell division in callus).
✓Not surprisingly, the term "auxin" is derived from the Greek
word "to increase or grow"
✓This was the first group of plant hormones discovered.
Aux.....
site of synthesis and transport
• Auxin is made in actively growing tissue which includes young
leaves, fruits, and especially the shoot apex.
• Made in cytosol of cells .
• Basipetal (or Polar) Transport Auxin is transported in a
basipetal (towards the base, base-seeking) direction.
• In other words, auxin moves from the shoot tip towards the
roots and from the root tip towards the shoot.
Auxin Actions
1. Cellular Elongation:
▪ Auxin can induce and amplify proton pumping.
▪ Acidified cell walls have increased elasticity which
lead to cell elongation
Auxin Actions cont....
2. Cell differentiation
➢Auxin promotes differentiation of vascular tissue (i.e.,
xylem & phloem):
➢Auxin and sugar -----> Vascular tissue
➢Auxin and low sugar (1.5 - 2.5%) -----> Xylem
➢Auxin and high sugar (4%) ------->- Phloem
➢Auxin and moderate levels of sugar (2.5 - 3.0%) ----->-
Xylem & Phloem
Auxin Actions cont....
3. Ethylene production
IAA apparently stimulates the production of
ethylene.
4. Inhibition of root growth
[IAA] > 10-6 M inhibit root elongation.
However, very low [IAA] (>10-8 M) favor root
elongation.
5. Stimulate root initiation (lateral roots,
adventitious roots)
Roots always form at the basal end of cutting
Auxin Actions cont....
➢Formation of adventitious roots
Auxin Actions cont....
6. Flowering
Although most plants don’t initiate the production of flowers
after auxin treatment, pineapple and its relatives (Bromeliaceae)
do. Once flowers are initiated, in many species, IAA promotes the
formation of female flowers.
Auxin Actions cont....
7. Parthenocarpic fruit development
 Pollination of the flowers of angiosperms initiates
the formation of seeds.
 As the seeds mature, they release auxin to the
surrounding flower parts, which develop into the
fruit that covers the seeds.
 Some commercial growers deliberately initiate fruit development
by applying auxin to the flowers. Not only does this ensure that all the
flowers will "set“ fruit, but it also maximizes the likelihood that all the
fruits will be ready for harvest at the same time.
Auxin Actions cont....
➢8- Apical Dominance
❖ Lateral branch growth are inhibited near the shoot
apex, but less so farther from the tip.
❖ Apical dominance is disrupted in some plants by
removing the shoot tip, causing the plant to become
bushy.
Auxin Actions cont....
• 10- Tropic responses
Such as gravitropism and phototropism
A-Phototropism
 is a growth movement induced by a light
stimulus
Phototropism
Sunlight breaks down auxin
Plant stems indirect sunlight will have the
least amount of auxin
Area of the plant that is more shaded will
have more auxin
More cell growth on shaded side
Plant bends towards light
Auxin Actions cont....
Auxin Actions cont....
• Geotropism or Gravitropism
 The plant stem that was once upright is on its side
 The auxin are settle on the bottom side of the stem
 More auxin accumulate on the stems bottom side
 More cell growth occurs on bottom side
 Plant bends upward
 A growth response to gravity which causes roots to
grow downward and shoots to grow upward
Gibberellins
Gibberellins cont..
Gibberellins are plant hormones that promote
growth, seed germination and leaf expansion.
They occur at low concentrations in vegetative tissues but at
higher concentrations in germinating seeds.
Induce cell elongation and cell division.
Important for plant growth and development through
flowering and/or seed germination.
Gibberellins cont..
• Site :
 Young leaves, roots, and developing seeds
(developing endosperm) and fruits.
Transport :
 Made in the tissue in which it is used
 Transport occurs through xylem, phloem, or cell-tocell.
 Phloem seems to be most important transport route
 Transport is not polar, as it is for auxin.
Gibberellins Actions
1- Promotes stem elongation
When applied to intact plants, GA usually causes an
increase, unlike auxin.
 It overcomes dwarfism in mutants that have a
mutation in the GA synthesis pathway.
dwarf = short;
wild type = tall ;
dwarf + GA = tall.
 Thus, GA application:
(1) stimulates elongation; and
(2) acts on intact plants.
Gibberellins Actions
• 2- Overcomes dormancy in seeds
 Gibberellins also have a fundamental role in breaking seed
dormancy and stimulating germination.
 The endosperm of many seeds contains protein and
carbohydrate reserves upon which a developing embryo
relies for energy and nutrition.
 These reserves must be mobilised and transported to the
embryo.
Gibberellins Actions
3- Involved in parthenocarpic fruit development
Gibberellins Actions
4- GA can induce fruit enlargement
External
application of
gibberellins can
also enlarge fruit
size in grapes
Gibberellins Actions
• 7-Sex expression
In plants with separate male and female
flowers, GA application can determine sex.
 For example, in cucumber and spinach, GA
treatment increases the proportion of male
flowers.
 In maize, GA treatment causes female flower
development
Cytokinins
• Cytokinins are hormones that stimulate cell
division, or cytokinesis
These hormones may also be involved in
controlling leaf senescence and the growth of
lateral branches
The most active, naturally-occurring cytokinin is
zeatin.
Cytokinins occur in most plants including mosses,
ferns, conifers, algae and diatoms
Cytokininns cont......
• Site:
Synthesized primarily in the meristematic region
of the roots.
This is known in part because roots can be
cultured (grown in Artificial medium in a flask)
without added cytokinin, but stem cells cannot.
 Cytokinins are also produced in developing
embryos
• Transport:
 Via xylem (transpiration stream).
 Zeatin ribosides are the main transport form;
converted to the free base or glucosides in the
leaves.
 Some cytokinin also moves in the phloem.
Cytokinins Actions
1- Control morphogenesis
2- Regulates the cell cycle/cell division
3- Bud development
4- Delay senescence
5- Greening
Cytokinins promotes the light-induced formation of chlorophyll
and conversion of etioplasts to chloroplasts (greening process)
Abscisic acid
• Inhibits growth
• Promotes dormancy
• Closes stomata
• Produced in response to stress.
Sites :
Plastids
Most tissues, especially leaves and seeds
Transport :
Xylem and phloem (greater amounts)
ABA Functions
• drought resistance
• Seed Dormancy
• freezing resistance
Ethylene
Ethylene is the only gaseous plant hormone (C2H4)
It is produced naturally by higher plants and is able to
diffuse readily, via intercellular spaces, throughout
the entire plant body
 Ethylene is involved primarily in plant responses to
environmental stresses such as flooding and drought,
and in response to infection, wounding and
mechanical pressure
Ethylene Action
➢ Ethylene— signal transduction
➢ Ethylene— fruit ripening
➢Ethylene— Shoot Growth
➢Ethylene— flowering
summary
• hormones an organic compound produced naturally in higher plants
• Auxin, GAs, cytokinins, ABA, and ethylene are the most common
PGRH.
• The are active in very low(10-6 to 10-5M),
• And it might be promotors or inhibitors
• And each has distinct features
• It is very rarely, if ever, that the plant hormones work alone; rather
plant growth and development usually result from interactions of
plant hormones
Physiology presentation1

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Physiology presentation1

  • 1. ROLE OF PLANT HORMONES
  • 2. Outline of the presentations ➢ Introduction ➢ Literature review ➢ Conclusion
  • 3. introduction ➢They are various organic compounds other than nutrients produced by plants that control or regulate germination, growth, metabolism, or other physiological activities. ➢Also called phytohormone and recently called growth bioregulators.
  • 5. Intro.... ➢Plant hormones, which are active in very low concentrations, are produced in certain parts of the plants and are usually transported to other parts where they elicit specific biochemical, physiological, or morphological responses. And hey are also active in tissues where they are produced. ➢ Each plant hormone evokes many different responses. Also, the effects of different hormones overlap and may be stimulatory or inhibitory . ➢The commonly recognized classes of plant hormones are the auxins, gibberellins, cytokinin, abscisic acid, and ethylene.
  • 6. Intro.... ❖Each hormone performs its specific functions; ❖ however, nearly all of the measurable responses of plants to heredity or environment are controlled by interaction between two or more hormones . ❖Such interactions may occur at various levels, including a. The synthesis of hormones, b. Hormone receptors, and second messengers, c. Ultimate hormone action.
  • 7. Intro... Plant hormones (or plant growth regulators, or PGRs) are internally secreted chemicals in plants that are used for regulating the plants’ growth. According to a standard definition, plant hormones are: ↔Signal molecules produced at specific locations, that occur in very low concentrations, and cause altered processes in target cells at other locations.
  • 8. Characteristics The concentration of hormones required for the plant response is very low(10-6 to 10-5M), comparing with the requirement of mineral and vitamin for plants. The synthesis of plant hormones is more diffuse and not always localized.
  • 9. Classifications of plant hormones It is accepted that there are two major classes of plant hormones: class action examples PROMOTORS Cause faster growth Auxins Cytokinin(cks) Gibberellins (gas) Brassinosteroids INHIBITORS Reduce growth Ethylene Abscisic acid (ABA)
  • 10. What do hormones control in plants? ➢Roots and shoots growth ➢Seed germination ➢ Leaf fall ➢Disease resistance, and water stress ➢Fruit formation and ripening ➢Flowering time ➢ Bud formation ➢Anything related to plant growth!
  • 14. Aux..... ✓Auxin is a general name for a group of hormones that are involved with growth responses (i.e., elongate cells, stimulate cell division in callus). ✓Not surprisingly, the term "auxin" is derived from the Greek word "to increase or grow" ✓This was the first group of plant hormones discovered.
  • 16. site of synthesis and transport • Auxin is made in actively growing tissue which includes young leaves, fruits, and especially the shoot apex. • Made in cytosol of cells . • Basipetal (or Polar) Transport Auxin is transported in a basipetal (towards the base, base-seeking) direction. • In other words, auxin moves from the shoot tip towards the roots and from the root tip towards the shoot.
  • 17. Auxin Actions 1. Cellular Elongation: ▪ Auxin can induce and amplify proton pumping. ▪ Acidified cell walls have increased elasticity which lead to cell elongation
  • 18. Auxin Actions cont.... 2. Cell differentiation ➢Auxin promotes differentiation of vascular tissue (i.e., xylem & phloem): ➢Auxin and sugar -----> Vascular tissue ➢Auxin and low sugar (1.5 - 2.5%) -----> Xylem ➢Auxin and high sugar (4%) ------->- Phloem ➢Auxin and moderate levels of sugar (2.5 - 3.0%) ----->- Xylem & Phloem
  • 19. Auxin Actions cont.... 3. Ethylene production IAA apparently stimulates the production of ethylene. 4. Inhibition of root growth [IAA] > 10-6 M inhibit root elongation. However, very low [IAA] (>10-8 M) favor root elongation. 5. Stimulate root initiation (lateral roots, adventitious roots) Roots always form at the basal end of cutting
  • 20. Auxin Actions cont.... ➢Formation of adventitious roots
  • 21. Auxin Actions cont.... 6. Flowering Although most plants don’t initiate the production of flowers after auxin treatment, pineapple and its relatives (Bromeliaceae) do. Once flowers are initiated, in many species, IAA promotes the formation of female flowers.
  • 22. Auxin Actions cont.... 7. Parthenocarpic fruit development  Pollination of the flowers of angiosperms initiates the formation of seeds.  As the seeds mature, they release auxin to the surrounding flower parts, which develop into the fruit that covers the seeds.  Some commercial growers deliberately initiate fruit development by applying auxin to the flowers. Not only does this ensure that all the flowers will "set“ fruit, but it also maximizes the likelihood that all the fruits will be ready for harvest at the same time.
  • 23. Auxin Actions cont.... ➢8- Apical Dominance ❖ Lateral branch growth are inhibited near the shoot apex, but less so farther from the tip. ❖ Apical dominance is disrupted in some plants by removing the shoot tip, causing the plant to become bushy.
  • 24. Auxin Actions cont.... • 10- Tropic responses Such as gravitropism and phototropism A-Phototropism  is a growth movement induced by a light stimulus Phototropism Sunlight breaks down auxin Plant stems indirect sunlight will have the least amount of auxin Area of the plant that is more shaded will have more auxin More cell growth on shaded side Plant bends towards light
  • 26. Auxin Actions cont.... • Geotropism or Gravitropism  The plant stem that was once upright is on its side  The auxin are settle on the bottom side of the stem  More auxin accumulate on the stems bottom side  More cell growth occurs on bottom side  Plant bends upward  A growth response to gravity which causes roots to grow downward and shoots to grow upward
  • 28. Gibberellins cont.. Gibberellins are plant hormones that promote growth, seed germination and leaf expansion. They occur at low concentrations in vegetative tissues but at higher concentrations in germinating seeds. Induce cell elongation and cell division. Important for plant growth and development through flowering and/or seed germination.
  • 29. Gibberellins cont.. • Site :  Young leaves, roots, and developing seeds (developing endosperm) and fruits. Transport :  Made in the tissue in which it is used  Transport occurs through xylem, phloem, or cell-tocell.  Phloem seems to be most important transport route  Transport is not polar, as it is for auxin.
  • 30. Gibberellins Actions 1- Promotes stem elongation When applied to intact plants, GA usually causes an increase, unlike auxin.  It overcomes dwarfism in mutants that have a mutation in the GA synthesis pathway. dwarf = short; wild type = tall ; dwarf + GA = tall.  Thus, GA application: (1) stimulates elongation; and (2) acts on intact plants.
  • 31. Gibberellins Actions • 2- Overcomes dormancy in seeds  Gibberellins also have a fundamental role in breaking seed dormancy and stimulating germination.  The endosperm of many seeds contains protein and carbohydrate reserves upon which a developing embryo relies for energy and nutrition.  These reserves must be mobilised and transported to the embryo.
  • 32. Gibberellins Actions 3- Involved in parthenocarpic fruit development
  • 33. Gibberellins Actions 4- GA can induce fruit enlargement External application of gibberellins can also enlarge fruit size in grapes
  • 34. Gibberellins Actions • 7-Sex expression In plants with separate male and female flowers, GA application can determine sex.  For example, in cucumber and spinach, GA treatment increases the proportion of male flowers.  In maize, GA treatment causes female flower development
  • 35. Cytokinins • Cytokinins are hormones that stimulate cell division, or cytokinesis These hormones may also be involved in controlling leaf senescence and the growth of lateral branches The most active, naturally-occurring cytokinin is zeatin. Cytokinins occur in most plants including mosses, ferns, conifers, algae and diatoms
  • 36. Cytokininns cont...... • Site: Synthesized primarily in the meristematic region of the roots. This is known in part because roots can be cultured (grown in Artificial medium in a flask) without added cytokinin, but stem cells cannot.  Cytokinins are also produced in developing embryos • Transport:  Via xylem (transpiration stream).  Zeatin ribosides are the main transport form; converted to the free base or glucosides in the leaves.  Some cytokinin also moves in the phloem.
  • 37. Cytokinins Actions 1- Control morphogenesis 2- Regulates the cell cycle/cell division 3- Bud development 4- Delay senescence 5- Greening Cytokinins promotes the light-induced formation of chlorophyll and conversion of etioplasts to chloroplasts (greening process)
  • 38. Abscisic acid • Inhibits growth • Promotes dormancy • Closes stomata • Produced in response to stress. Sites : Plastids Most tissues, especially leaves and seeds Transport : Xylem and phloem (greater amounts)
  • 39. ABA Functions • drought resistance • Seed Dormancy • freezing resistance
  • 40. Ethylene Ethylene is the only gaseous plant hormone (C2H4) It is produced naturally by higher plants and is able to diffuse readily, via intercellular spaces, throughout the entire plant body  Ethylene is involved primarily in plant responses to environmental stresses such as flooding and drought, and in response to infection, wounding and mechanical pressure
  • 41. Ethylene Action ➢ Ethylene— signal transduction ➢ Ethylene— fruit ripening ➢Ethylene— Shoot Growth ➢Ethylene— flowering
  • 42. summary • hormones an organic compound produced naturally in higher plants • Auxin, GAs, cytokinins, ABA, and ethylene are the most common PGRH. • The are active in very low(10-6 to 10-5M), • And it might be promotors or inhibitors • And each has distinct features • It is very rarely, if ever, that the plant hormones work alone; rather plant growth and development usually result from interactions of plant hormones