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Obj 4: Improve chickpea productivity for
           marginal environments in sub-Saharan Africa
                         and Asia-Kenya

             Progress of Data management -
                         Kenya




Paul Kimurto
Egerton University

                 TL-I Annual Meeting
            9-15 April 2012, Addis, Ethiopia
Partners involved


Institution          Key scientists
Egerton University   Paul Kimurto,Richard
                 Mulwa, Bernard Towett ,
                   Alice Kosgei, Serah Songok,
                   Moses Oyier, Oyoo
ICRISAT-India   Rajeev Varshney, Pooran Gaur,
                Thudi Mahender, Trushar Shar,
                 Prasad
ICRISAT-Nairobi Ganga Rao, Said Silim, Kaloki
                Peter
Status of chickpea Data sets
                Progress -Phase I (2007-10) & Phase II (2011-2012)
• Activity 3: INSECT RESISTANCE- Phenotyping reference sets for
  insect resistance

• Activity 4: DROUGHT TOLERANCE-Phenotyping selected minicore
  samples for drought tolerance



• Activity 5: MODERN BREEDING-Improve locally adapted germplasm
  for drought tolerance and yield traits through modern breeding
Activity 3 & 4: Insect & Drought tolerance

Activity 3; Insect Resistance screening

Screening of International Helicoverpa resistance nursery of 30 lines
in Kenya (2008/2009)

Data submitted to TL1 chickpea data manager

Activity 4; Drought tolerance
1. Phenotyping of 289 genotypes of reference and mapping
population for drought tolerance, HI, seed size and yield in 2
locations Kenya 2007-2009

Data submitted to TL1 chickpea data manager

2. Phenotyping of 80 genotypes for yield, HI, seed size and yield in
2 locations (root reference set) 2008-2009

Data submitted to TL1 chickpea data manager
Activity 3 & 4: Insect & Drought tolerance


3. Phenotyping 140 advanced chickpea breeding lines for
Helicoverpa armigera resistance in 2 sites –under TLII Msc
student-Nancy Njogu-Msc student (2010-2011)

Data completed to be submitted to Data manager in 2012
Activity 5: Improve locally adapted
              germplasm for target traits through
              modern breeding
1. Introgression of QTL root traits using MABC for improving
   drought tolerance

•   ICCV 97105 (recipient parent) x ICC 4958 (donor
    parent)
       -BC3F2 & BC4F1 progenies available to be evaluated
       in 2 locations in 2012

•   ICCV 95423 x ICC 4958 and ICCV 10 x ICC 4958
    • -BC3F4 progenies available for evaluation in April
       2012 in 2 locations

Genotypic and phenotpic data to be submitted to Data
manager 2012
Activity 5: MABC Contd…..


2. Multi-location evaluation BC3F4 lines-24 lines of JG 11 x
ICC 4958 in 2 locations in Kenya (Nov 2011-March 2012)

Data is being processed for submission to data manager
Marker-assisted recurrent selection
                            (MARS)
• 2. Application of MARS
•   Good parental cross
•    JG 11 x ICCV 04112
•    JG 130 x ICCV 05107
•    Phenotyping of 188 progenies of each population of F3:5
     and F3:6 lines in 2 seasons (2010/2012)

• After genotyping and phenotyping -Recombination cycle of
  best 8 progenies will be done to enhance yield traits



• Data is being processed for submission to data manager
Status of Tablet Computer

•Tablet computer was received in 2011 in Hyderabad
during TLI AGM


•Planned to use in April 2012 planting season to prepare
Nursery books and Fieldbooks

•Challenge-Data coding of germplasm list was needed we
could not transfer directly from excel sheet but needed
coding

•Consulted Akinnola and now it will be used in second site
trial-May 2012
Status of Tablet Computer

         Suitability for use in trials
•Overall use in preparation and setting up of trials is very
good

   •Inputing field layout, sites, location, partners etc
   makes work easy and reduces errors

   •No need for printing field books in advance

   •Can be replicated by many users


•Good especially in reducing manual randomization
Status of Tablet Computer

         Suitability for use in trials
•Import and export functions-Loading of traits to be
measured from Trait dictionary makes preparation for
trials very easy and effective


•Data entry is easy especially with touch screen

•Large and Excellent screen for taking photos


•Battery hours is adequate
Status of Tablet Computer

•Limitations

•Under bright sunshine feasibility is low-readings a
problem

•Delicate and can break


•Fields are dirty and tablet requires a lot of
neatness from user
Thanks alot

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TLI 2012: Data management for chickpea breeding - Kenya

  • 1. Obj 4: Improve chickpea productivity for marginal environments in sub-Saharan Africa and Asia-Kenya Progress of Data management - Kenya Paul Kimurto Egerton University TL-I Annual Meeting 9-15 April 2012, Addis, Ethiopia
  • 2. Partners involved Institution Key scientists Egerton University Paul Kimurto,Richard Mulwa, Bernard Towett , Alice Kosgei, Serah Songok, Moses Oyier, Oyoo ICRISAT-India Rajeev Varshney, Pooran Gaur, Thudi Mahender, Trushar Shar, Prasad ICRISAT-Nairobi Ganga Rao, Said Silim, Kaloki Peter
  • 3. Status of chickpea Data sets Progress -Phase I (2007-10) & Phase II (2011-2012) • Activity 3: INSECT RESISTANCE- Phenotyping reference sets for insect resistance • Activity 4: DROUGHT TOLERANCE-Phenotyping selected minicore samples for drought tolerance • Activity 5: MODERN BREEDING-Improve locally adapted germplasm for drought tolerance and yield traits through modern breeding
  • 4. Activity 3 & 4: Insect & Drought tolerance Activity 3; Insect Resistance screening Screening of International Helicoverpa resistance nursery of 30 lines in Kenya (2008/2009) Data submitted to TL1 chickpea data manager Activity 4; Drought tolerance 1. Phenotyping of 289 genotypes of reference and mapping population for drought tolerance, HI, seed size and yield in 2 locations Kenya 2007-2009 Data submitted to TL1 chickpea data manager 2. Phenotyping of 80 genotypes for yield, HI, seed size and yield in 2 locations (root reference set) 2008-2009 Data submitted to TL1 chickpea data manager
  • 5. Activity 3 & 4: Insect & Drought tolerance 3. Phenotyping 140 advanced chickpea breeding lines for Helicoverpa armigera resistance in 2 sites –under TLII Msc student-Nancy Njogu-Msc student (2010-2011) Data completed to be submitted to Data manager in 2012
  • 6. Activity 5: Improve locally adapted germplasm for target traits through modern breeding 1. Introgression of QTL root traits using MABC for improving drought tolerance • ICCV 97105 (recipient parent) x ICC 4958 (donor parent) -BC3F2 & BC4F1 progenies available to be evaluated in 2 locations in 2012 • ICCV 95423 x ICC 4958 and ICCV 10 x ICC 4958 • -BC3F4 progenies available for evaluation in April 2012 in 2 locations Genotypic and phenotpic data to be submitted to Data manager 2012
  • 7. Activity 5: MABC Contd….. 2. Multi-location evaluation BC3F4 lines-24 lines of JG 11 x ICC 4958 in 2 locations in Kenya (Nov 2011-March 2012) Data is being processed for submission to data manager
  • 8. Marker-assisted recurrent selection (MARS) • 2. Application of MARS • Good parental cross • JG 11 x ICCV 04112 • JG 130 x ICCV 05107 • Phenotyping of 188 progenies of each population of F3:5 and F3:6 lines in 2 seasons (2010/2012) • After genotyping and phenotyping -Recombination cycle of best 8 progenies will be done to enhance yield traits • Data is being processed for submission to data manager
  • 9. Status of Tablet Computer •Tablet computer was received in 2011 in Hyderabad during TLI AGM •Planned to use in April 2012 planting season to prepare Nursery books and Fieldbooks •Challenge-Data coding of germplasm list was needed we could not transfer directly from excel sheet but needed coding •Consulted Akinnola and now it will be used in second site trial-May 2012
  • 10. Status of Tablet Computer Suitability for use in trials •Overall use in preparation and setting up of trials is very good •Inputing field layout, sites, location, partners etc makes work easy and reduces errors •No need for printing field books in advance •Can be replicated by many users •Good especially in reducing manual randomization
  • 11. Status of Tablet Computer Suitability for use in trials •Import and export functions-Loading of traits to be measured from Trait dictionary makes preparation for trials very easy and effective •Data entry is easy especially with touch screen •Large and Excellent screen for taking photos •Battery hours is adequate
  • 12. Status of Tablet Computer •Limitations •Under bright sunshine feasibility is low-readings a problem •Delicate and can break •Fields are dirty and tablet requires a lot of neatness from user