Osmosis is the net diffusion of water molecules from a dilute solution into a more concentrated solution through a semi-permeable membrane due to a concentration gradient. Osmotic pressure is the excess pressure that must be applied to a solution to prevent the passage of solvent into the solution, and it is measured in units called osmoles. Osmotic pressure follows two laws: πV = nRT and π = cRT, where π is osmotic pressure, V is volume, n is moles of solute, R is the gas constant, T is temperature, and c is molarity. Applications of osmosis include water desalination through reverse osmosis, measuring

1. OSMOSIS
OSMOTIC PRESSURE
AND

2. FAWAD MUEEN ARBI
ROLL # 159
QUAID-E-AZAM MEDICAL COLLEGE
BAHAWALPUR

3. CONTENT LAYOUT
 Osmosis
• Introduction
• Explanation
 Osmotic Pressure
• Introduction
• Measurement of Osmotic Pressure
• Laws of Osmotic Pressure
 Applications

4. WHAT IS OSMOSIS?
The process of net diffusion of water
molecules from dilute solution or pure
water to more concentrated solution,
when both are separated by semi-
permeable membrane.

5. REASON OF ENTRY OF WATER
It is not because solute exerts pulling
effect on water but actually it is because
of the concentration gradient of solvent.

6. OSMOTIC PRESSURE

7. OSMOTIC PRESSURE
• It is the excess pressure that must be
applied to a solution to prevent the
passage of solvent into the solution
• It is a colligative property

8. MEASURMENT OF OSMOTIC
PRESSURE
Various Methods can be used but most
common are :
It is measured in Osmoles.
1. Pfeffer’s Method
2. Freezing Point Method

9. LAWS OF OSMOTIC PRESSURE
It is represented by Greek letter π :
πV = nRT
π = cRT
Where π is Osmotic Pressure
c is Molarity of Solution
R is General Gas Constant
T is Absolute Temprature

10. APPLICATIONS OF
OSMOSIS

11. ADD A SLIDE TITLE - 1
Water Desalination principle is
based on Reverse Osmosis

12. RBC Fragility Test is based on Osmosis

13. Adding Salt to an injury causes
pain because of Osmosis

14. Pruned Fingers are due to Osmosis

15. ADD A SLIDE TITLE - 3
Vibrio Cholerae’s Action
Mechanism is through Osmosis

16. REFERENCES
• Medical Biochemistry 8th Edition by MN
Chatterjea (Page # 818-819)
• Essentials of Biochemistry Vol. 1 8th
Edition by Mushtaq Ahmed (Page # 46-
49)
• Biochemistry 5th Edition by U.
Satyanarayana (Page # 681-682)

17. ACKNOWLEDGMENT
• Allah SWT
• Hazrat Muhammad ‫ﷺ‬
• Thank-you to Biochemistry Department
for giving me this opportunity to present
my work