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Tannins, properties
and uses
Student: Carla De Souza
Supervisor: Nidhi Bansal
Severino Gonzaga Neto
What are?
โ€ข Tannins are phenolic plant secondary compounds and
are widely distributed through the plant kingdom,
especially legumes and browse which affect animal
performance in many countries.
โ€ข Myriad of supplemental
functions in a plantโ€™s life
cycle
โ€ข Protective tissues
โ€ข Defense strategies
Why?
Anthocyanins
Isoflavonoids
Death of plants - phenolic compounds may persist for
weeks or months and affect decomposer organisms
and decomposition processes in soils.
Not restricted to only
the plant - extend to the
functioning of whole
ecosystems.
earthworm
Uses
โ€ข Dyes
โ€ข Fibers
โ€ข Glues
โ€ข Oils
โ€ข Waxes
โ€ข Flavoring agents
โ€ข Drugs
โ€ข Perfumes
โ€ข Potential sources of new natural drugs, antibiotics,
insecticides and herbicides
Natural drugs, antibiotics, insecticides and
herbicides
โ€ข Unlike the traditional vitamins
โ€ข Not essential for short-term
โ€ข Modest long-term intakes
โ€ข Modulating human metabolism - prevention or
reduction - degenerative diseases such as:
๏ƒ˜ Cardiovascular diseases
๏ƒ˜ Diabetes
๏ƒ˜ Obesity
๏ƒ˜ Cancer
Benefitโ€™s sources
โ€ข Tree bark
โ€ข Grains
โ€ข Legumes
โ€ข Fruits
โ€ข Vegetables
โ€ข Wine
โ€ข Milk
Milk
โ€ข Soy milk
โ€ข Cow milk
Cow milk
โ€ข Feeding the cows
โ€ข Milk fortification
Feeding the cows
โ€ข Sorghum grain
Comparatively containing
โ€ข 90 to 95% of corn NV, in EV
โ€ข Protein content of around 8 to 9%,
corn, although with lesser quality
โ€ข Methionine and lysine โ€“ maize,
tryptophan.
โ€ข Contain tannins
Tannins
โ€ข Tannins are toxic to ruminants ?
โ€ข Performance is impaired by tannins ingestion?
Harmful, harmless or beneficial - type and chemical
structure, species, the amount ingested and the animal
Types of tannins
The binding ability to proteins, especially to protect
the protein from rumen fermentation allow better
utilization of dietary protein in ruminant animals
Types of tannins
โ€ข The tannins divided into 3 subclasses:
โ€ข Condensed tannins or proanthocyanidins,
โ€ข Hydrolysable tannins,
โ€ข Derived tannins
โ€ข Condensed tannins
โ€ข Hydrolysable tannins
Figure- Structure of flavan-3-ol monomers and dimers. (A)-Epicatechin with
R1=OH and R2=H or (+)-catechin with R1=H and R2=OH;, (B) procyanidin (4b-8)-
dimer; (C) procyanidin (4b-6)-dimer.
Condensed tannins
flavan-3-ols
Proanthocyanidins
โ€ข Presence in food affects quality parameters such as
๏ƒ˜ Astringency
๏ƒ˜ Bitterness
๏ƒ˜ Sourness
๏ƒ˜ Sweetness
๏ƒ˜ Salivary viscosity
๏ƒ˜ Aroma
๏ƒ˜ Color formation
๏ƒ˜ The binding ability to proteins
Parametres
Condensed tannins (Acacia mearnsii)
๏ƒ˜ Molecular mass of on average 1250 units
๏ƒ˜ Completely water soluble
๏ƒ˜ Brown free flowing powder (68.3% tannin)
๏ƒ˜ pH 4.8
CONTI, et al., Revista Argentina de Producciรณn Animal (2007).
Production, milk composition and changes in cows body weight at grazing and
suplemented with condensed tannins (Bioquina ยฎ)
KUSHWAHA, et al., Agri. Reviews, 2011.
โ€ข Effects of tannins on milk yield and milk composition Tannins
can be used as feed additive to reduce the degradation of
protein in the rumen.
โ€ข Tannins form complexes with protein that are less soluble and
accessible to proteolytic enzymes in the rumen. A non-
significant increase in milk production was observed in
lactating cows fed on 10% salseed meal (11% ta) in concentrate
mixture.
โ€ข Inclusion of 10 and 20% salseed meal didโ€™nt affect the health of
the animal and rumen fermentation pattern.
โ€ข No significant difference in milk yield obtained in untreated,
10% tannic acid as well as 1% formaldehyde treated groundnut
cake in lactating goats.
Effect of T. chebula extract supplementation on plasma fatty acid profile (g/100 g of FAME)
of kids.
RANA et al., Meat Science (2012).
โ€ข The effectiveness of polyphenols depends on
preserving the stability, bioactivity and bioavailability
of the active ingredients.
โ€ข The unpleasant taste of most phenolic compounds
also limits their application.
โ€ข The utilization of encapsulated polyphenols, instead
of free compounds, can effectively alleviate these
deficiencies (FANG and BHANDARI).
โ€ข In the food industry, the microencapsulation
process can be applied for a variety of
reasons, one of them is mask an unwanted
flavor or taste of the core material
RANA et al., Effect of tanniniferous Terminalia chebula extract on rumen
biohydrogenation, ฮ”9-desaturase activity, CLA content and fatty acid composition in
longissimus dorsi muscle of kids. Meat Science 90 (2012) 558โ€“563.
Raju Kushwaha, S.N. Rai, A.K. Singh, G. Chandra, M.M. Vaidya,
V.K. Sharma, M.M. Pathan' and S. Kumar. TANNINIFEROUS FEED RESOURCES IN DAIRY
ANIMALS: A REVIEW. Agri. Reviews, 32 (4) : 267 - 275, 2011
Conti, et al., Efecto de los taninos condensados sobre la producciรณn y composiciรณn de
leche de vacas lecheras en pastoreo de verano. Fac.Cs.Vet., UNLitoral. Revista Argentina
de Producciรณn Animal Vol 27 Supl. 1 (2007).
Thanks

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Tannins

  • 1. Tannins, properties and uses Student: Carla De Souza Supervisor: Nidhi Bansal Severino Gonzaga Neto
  • 2. What are? โ€ข Tannins are phenolic plant secondary compounds and are widely distributed through the plant kingdom, especially legumes and browse which affect animal performance in many countries.
  • 3. โ€ข Myriad of supplemental functions in a plantโ€™s life cycle โ€ข Protective tissues โ€ข Defense strategies Why? Anthocyanins Isoflavonoids
  • 4. Death of plants - phenolic compounds may persist for weeks or months and affect decomposer organisms and decomposition processes in soils. Not restricted to only the plant - extend to the functioning of whole ecosystems. earthworm
  • 5. Uses โ€ข Dyes โ€ข Fibers โ€ข Glues โ€ข Oils โ€ข Waxes โ€ข Flavoring agents โ€ข Drugs โ€ข Perfumes โ€ข Potential sources of new natural drugs, antibiotics, insecticides and herbicides
  • 6. Natural drugs, antibiotics, insecticides and herbicides โ€ข Unlike the traditional vitamins โ€ข Not essential for short-term โ€ข Modest long-term intakes โ€ข Modulating human metabolism - prevention or reduction - degenerative diseases such as: ๏ƒ˜ Cardiovascular diseases ๏ƒ˜ Diabetes ๏ƒ˜ Obesity ๏ƒ˜ Cancer
  • 7. Benefitโ€™s sources โ€ข Tree bark โ€ข Grains โ€ข Legumes โ€ข Fruits โ€ข Vegetables โ€ข Wine โ€ข Milk
  • 9. Cow milk โ€ข Feeding the cows โ€ข Milk fortification
  • 10. Feeding the cows โ€ข Sorghum grain Comparatively containing โ€ข 90 to 95% of corn NV, in EV โ€ข Protein content of around 8 to 9%, corn, although with lesser quality โ€ข Methionine and lysine โ€“ maize, tryptophan. โ€ข Contain tannins
  • 11. Tannins โ€ข Tannins are toxic to ruminants ? โ€ข Performance is impaired by tannins ingestion? Harmful, harmless or beneficial - type and chemical structure, species, the amount ingested and the animal Types of tannins The binding ability to proteins, especially to protect the protein from rumen fermentation allow better utilization of dietary protein in ruminant animals
  • 12. Types of tannins โ€ข The tannins divided into 3 subclasses: โ€ข Condensed tannins or proanthocyanidins, โ€ข Hydrolysable tannins, โ€ข Derived tannins โ€ข Condensed tannins โ€ข Hydrolysable tannins
  • 13. Figure- Structure of flavan-3-ol monomers and dimers. (A)-Epicatechin with R1=OH and R2=H or (+)-catechin with R1=H and R2=OH;, (B) procyanidin (4b-8)- dimer; (C) procyanidin (4b-6)-dimer. Condensed tannins flavan-3-ols Proanthocyanidins
  • 14. โ€ข Presence in food affects quality parameters such as ๏ƒ˜ Astringency ๏ƒ˜ Bitterness ๏ƒ˜ Sourness ๏ƒ˜ Sweetness ๏ƒ˜ Salivary viscosity ๏ƒ˜ Aroma ๏ƒ˜ Color formation ๏ƒ˜ The binding ability to proteins Parametres
  • 15. Condensed tannins (Acacia mearnsii) ๏ƒ˜ Molecular mass of on average 1250 units ๏ƒ˜ Completely water soluble ๏ƒ˜ Brown free flowing powder (68.3% tannin) ๏ƒ˜ pH 4.8
  • 16. CONTI, et al., Revista Argentina de Producciรณn Animal (2007). Production, milk composition and changes in cows body weight at grazing and suplemented with condensed tannins (Bioquina ยฎ)
  • 17. KUSHWAHA, et al., Agri. Reviews, 2011. โ€ข Effects of tannins on milk yield and milk composition Tannins can be used as feed additive to reduce the degradation of protein in the rumen. โ€ข Tannins form complexes with protein that are less soluble and accessible to proteolytic enzymes in the rumen. A non- significant increase in milk production was observed in lactating cows fed on 10% salseed meal (11% ta) in concentrate mixture. โ€ข Inclusion of 10 and 20% salseed meal didโ€™nt affect the health of the animal and rumen fermentation pattern. โ€ข No significant difference in milk yield obtained in untreated, 10% tannic acid as well as 1% formaldehyde treated groundnut cake in lactating goats.
  • 18. Effect of T. chebula extract supplementation on plasma fatty acid profile (g/100 g of FAME) of kids. RANA et al., Meat Science (2012).
  • 19.
  • 20. โ€ข The effectiveness of polyphenols depends on preserving the stability, bioactivity and bioavailability of the active ingredients. โ€ข The unpleasant taste of most phenolic compounds also limits their application. โ€ข The utilization of encapsulated polyphenols, instead of free compounds, can effectively alleviate these deficiencies (FANG and BHANDARI).
  • 21. โ€ข In the food industry, the microencapsulation process can be applied for a variety of reasons, one of them is mask an unwanted flavor or taste of the core material
  • 22. RANA et al., Effect of tanniniferous Terminalia chebula extract on rumen biohydrogenation, ฮ”9-desaturase activity, CLA content and fatty acid composition in longissimus dorsi muscle of kids. Meat Science 90 (2012) 558โ€“563. Raju Kushwaha, S.N. Rai, A.K. Singh, G. Chandra, M.M. Vaidya, V.K. Sharma, M.M. Pathan' and S. Kumar. TANNINIFEROUS FEED RESOURCES IN DAIRY ANIMALS: A REVIEW. Agri. Reviews, 32 (4) : 267 - 275, 2011 Conti, et al., Efecto de los taninos condensados sobre la producciรณn y composiciรณn de leche de vacas lecheras en pastoreo de verano. Fac.Cs.Vet., UNLitoral. Revista Argentina de Producciรณn Animal Vol 27 Supl. 1 (2007). Thanks