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GROWTH OF MICROALGAE IN
REGARDS TO BIODIESEL
PRODUCTION
Ben Peterson
CHME 486
𝐶𝑂2 Reduction
 In recent years there has been an increase in
global effort to reduce humanity’s carbon
footprint
 Governments are establishing new emission
reduction targets
 European Union’s target of between 10 and 20%
by 2020
 Germany’s suggestion of a 30% cut by 2020 if
matched by other nations
 United States in the range of 26 to 28 percent
below the 2005 levels by 2025
Alternative Technologies
 Transportation sector shown to have a total
greenhouse gas contribution of 78% to the
overall increase of emissions from 1979 to
2010
 Production of crop-derived biodiesel and
bioethanol
 Soybean
 Corn
 Oil palms
Microalgae
 Water based micro-organisms with 40,000
species already identified
 Convert fatty acids into lipids using a process
called esterification
Figure 1: Green algae
(Chlorophyceae) is
one of 40,000 species
of microalgae already
identified.
Optimal Microalgae Growth
Conditions
 Lighting conditions with waves between 450
nm and 650 nm in length while also
incorporating a dark cycle where little to no
light is present
 Temperature range of 25-35°C
 Water pH range between 7 and 9
 𝐶𝑂2 concentration of 350-1000 ppm with
adequate mixing
Current Growth Processes
 Several processes are currently being used to
grow microalgae for biodiesel production
 Utilize specific growth conditions to best
produce microalgae
 Suspended culture set up
 Immobilized cultures
 Open pond system
Open Pond System
 Shallow ponds which are uncovered and open
to the environment where microalgae is grown
 High rate algal pond also known as a raceway
pond
Figure 2: Algal raceways
are shallow ponds with
a paddle wheel used to
distribute nutrients for
microalgae growth.
Optimizing Production
 Possibility of genetically manipulating algae
through stress conditions in the growth
process
 Nitrogen starvation
 Produce microalgae at a maximum concentration
ranging between 2 g/L and 3.6 g/L
 Two of the species (Nannochloropsis sp., and
Newchloris oleabundans) showing around a 50%
increase in oil quantity
Wastewater Treatment Facilities
Figure 3: The Logan Lagoon waste water treatment facility located in Logan, Utah.
Utah State’s biological engineering department conducts algae based research
utilizing the facility.
Harvesting Microalgae
 There is no single best, current method used
to harvest microalgae
 Existing methods
 Flocculation
 Micro screening
 Centrifugation
 Future processing systems must utilize cost
and energy efficient designs to allow for the
feasibility of biodiesel produced from
microalgae
The RABR
Figure 4: The Rotating Algal Biofilm
Reactor (RABR) designed at Utah State
University.
 4000 ft of solid
braided cotton rope
wrapped around 74’’
diameter irrigation
wheels
 Combination of both
algal growth and
water treatment
Conclusions
 Microalgae has tremendous potential for future
production of biodiesel with hopes of reducing
the world’s dependence on fossil fuels
 Several methods of growing and harvesting of
microalgae are available
 Genetic manipulation of algae strains and the
development of efficient growing and
harvesting systems used to make microalgae
more economically feasible
References
 IPCC, 2014: Climate Change 2014: Synthesis Report. Contribution of Working Groups I, II and III to the Fifth
Assessment Report of the Intergovernmental Panel on Climate Change [Core Writing Team, R.K. Pachauri and
L.A. Meyer (eds.)]. IPCC, Geneva, Switzerland, 151 pp.
 Schenk, Peer M., Sky R. Thomas-Hall, Evan Stephens, Ute C. Marx, Jan H. Hussgnug, Clemens Posten, Olaf
Kruse, and Ben Hankamer. "Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel
Production." BioEnergy Research 1.1 (2008): 20-43. Springer Link. Web. 11 Apr. 2015.
 "President Obama's Plan to Fight Climate Change." The White House. The White House, n.d. Web. 25 Apr. 2015.
 "FACT SHEET: Clean Power Plan Overview." FACT SHEET: Clean Power Plan Overview. United States
Environmental Protection Agency, 13 June 2014. Web. 25 Apr. 2015.
 Chisti, Yusuf. "Biodiesel from Microalgae Beats Bioethanol." Trends in Biotechnology26.3 (2008): 126-31. Web. 11
Apr. 2015.
 Hu, Qiang, Milton Sommerfeld, Eric Jarvis, Maria Ghirardi, Matthew Posewitz, Michael Seibert, and Al Darzins.
"Microalgal Triacylglycerols as Feedstocks for Biofuel Production: Perspectives and Advances." The Plant
Journal 54.4 (2008): 621-39. Web. 11 Apr. 2015.
 Li Y, Wan C (2011) Algae for Biofuels.
 Christenson, Logan, and Ronald Sims, Dr. "Production and Harvesting of Microalgae for Wastewater Treatment,
Biofuels, and Bioproducts." Biotechnology Advances (2011): n. pag. Web. 11 Apr. 2015.
 Gouveia, Luisa, and Ana C. Gouveia. "Microalgae as a Raw Material for Biofuels Production." Journal of Industrial
Microbiology & Biotechnology 36.2 (2009): 269-74. Springer Link. Web. 11 Apr. 2015.
 Smith Terance, Sathish Ashik, Thompson Reese, Ronald Sims Dr. "Rotating Algal Biofilm Reactor (RABR) for
Biomass Growth and Nutrient Removal." Algae Biomass Summit, October 2014. PowerPoint presentation.
 Fresh-water Green Algae. Digital image. Introduction to the Chlorophyceae. University of Berkeley, 2006. Web. 25
Apr. 2015.
Questions?

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Biofuels Presentation

  • 1. GROWTH OF MICROALGAE IN REGARDS TO BIODIESEL PRODUCTION Ben Peterson CHME 486
  • 2. 𝐶𝑂2 Reduction  In recent years there has been an increase in global effort to reduce humanity’s carbon footprint  Governments are establishing new emission reduction targets  European Union’s target of between 10 and 20% by 2020  Germany’s suggestion of a 30% cut by 2020 if matched by other nations  United States in the range of 26 to 28 percent below the 2005 levels by 2025
  • 3. Alternative Technologies  Transportation sector shown to have a total greenhouse gas contribution of 78% to the overall increase of emissions from 1979 to 2010  Production of crop-derived biodiesel and bioethanol  Soybean  Corn  Oil palms
  • 4. Microalgae  Water based micro-organisms with 40,000 species already identified  Convert fatty acids into lipids using a process called esterification Figure 1: Green algae (Chlorophyceae) is one of 40,000 species of microalgae already identified.
  • 5. Optimal Microalgae Growth Conditions  Lighting conditions with waves between 450 nm and 650 nm in length while also incorporating a dark cycle where little to no light is present  Temperature range of 25-35°C  Water pH range between 7 and 9  𝐶𝑂2 concentration of 350-1000 ppm with adequate mixing
  • 6. Current Growth Processes  Several processes are currently being used to grow microalgae for biodiesel production  Utilize specific growth conditions to best produce microalgae  Suspended culture set up  Immobilized cultures  Open pond system
  • 7. Open Pond System  Shallow ponds which are uncovered and open to the environment where microalgae is grown  High rate algal pond also known as a raceway pond Figure 2: Algal raceways are shallow ponds with a paddle wheel used to distribute nutrients for microalgae growth.
  • 8. Optimizing Production  Possibility of genetically manipulating algae through stress conditions in the growth process  Nitrogen starvation  Produce microalgae at a maximum concentration ranging between 2 g/L and 3.6 g/L  Two of the species (Nannochloropsis sp., and Newchloris oleabundans) showing around a 50% increase in oil quantity
  • 9. Wastewater Treatment Facilities Figure 3: The Logan Lagoon waste water treatment facility located in Logan, Utah. Utah State’s biological engineering department conducts algae based research utilizing the facility.
  • 10. Harvesting Microalgae  There is no single best, current method used to harvest microalgae  Existing methods  Flocculation  Micro screening  Centrifugation  Future processing systems must utilize cost and energy efficient designs to allow for the feasibility of biodiesel produced from microalgae
  • 11. The RABR Figure 4: The Rotating Algal Biofilm Reactor (RABR) designed at Utah State University.  4000 ft of solid braided cotton rope wrapped around 74’’ diameter irrigation wheels  Combination of both algal growth and water treatment
  • 12. Conclusions  Microalgae has tremendous potential for future production of biodiesel with hopes of reducing the world’s dependence on fossil fuels  Several methods of growing and harvesting of microalgae are available  Genetic manipulation of algae strains and the development of efficient growing and harvesting systems used to make microalgae more economically feasible
  • 13. References  IPCC, 2014: Climate Change 2014: Synthesis Report. Contribution of Working Groups I, II and III to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change [Core Writing Team, R.K. Pachauri and L.A. Meyer (eds.)]. IPCC, Geneva, Switzerland, 151 pp.  Schenk, Peer M., Sky R. Thomas-Hall, Evan Stephens, Ute C. Marx, Jan H. Hussgnug, Clemens Posten, Olaf Kruse, and Ben Hankamer. "Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel Production." BioEnergy Research 1.1 (2008): 20-43. Springer Link. Web. 11 Apr. 2015.  "President Obama's Plan to Fight Climate Change." The White House. The White House, n.d. Web. 25 Apr. 2015.  "FACT SHEET: Clean Power Plan Overview." FACT SHEET: Clean Power Plan Overview. United States Environmental Protection Agency, 13 June 2014. Web. 25 Apr. 2015.  Chisti, Yusuf. "Biodiesel from Microalgae Beats Bioethanol." Trends in Biotechnology26.3 (2008): 126-31. Web. 11 Apr. 2015.  Hu, Qiang, Milton Sommerfeld, Eric Jarvis, Maria Ghirardi, Matthew Posewitz, Michael Seibert, and Al Darzins. "Microalgal Triacylglycerols as Feedstocks for Biofuel Production: Perspectives and Advances." The Plant Journal 54.4 (2008): 621-39. Web. 11 Apr. 2015.  Li Y, Wan C (2011) Algae for Biofuels.  Christenson, Logan, and Ronald Sims, Dr. "Production and Harvesting of Microalgae for Wastewater Treatment, Biofuels, and Bioproducts." Biotechnology Advances (2011): n. pag. Web. 11 Apr. 2015.  Gouveia, Luisa, and Ana C. Gouveia. "Microalgae as a Raw Material for Biofuels Production." Journal of Industrial Microbiology & Biotechnology 36.2 (2009): 269-74. Springer Link. Web. 11 Apr. 2015.  Smith Terance, Sathish Ashik, Thompson Reese, Ronald Sims Dr. "Rotating Algal Biofilm Reactor (RABR) for Biomass Growth and Nutrient Removal." Algae Biomass Summit, October 2014. PowerPoint presentation.  Fresh-water Green Algae. Digital image. Introduction to the Chlorophyceae. University of Berkeley, 2006. Web. 25 Apr. 2015.