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Agricultural Extension Journal 2019; 3(1):35-37
ISSN 2521 – 0408
RESEARCH ARTICLE
Assessment of Two Forage Sorghum-Sudan Grass Hybrids#
Gamar Alanbiaa Hag Musa Elamin1
, Ahmed Abdel Aziz Hamid Abdel Aziz2
1
Department of Crop Science, Faculty of Agriculture and Natural Resources, University of Bakht Al Ruda,
Ed Dueim, Sudan, 2
Department of Agronomy and Horticulture, Faculty of Agricultural Technology and Fish
Sciences, Al Neelain University, Khartoum, Sudan
#
Part of MSc thesis submitted by the first author
Received: 15-10-2018; Revised: 15-11-2018; Accepted: 01-02-2019
ABSTRACT
This trial was conducted for two consecutive Kharif seasons of 2011 and 2012 to compare two interspecific
hybrids of sorghum and Sudan grass, namely Mabrook and Panar 888 with two local checks Abu Sabein
andAbu Sabein Kambal. Cultivars were sown in a randomized complete block design with four replicates.
Data were collected on plant height, number of leaves per plant, leaf area, stem diameter, number of days
to 50 % flowering, plant density, fresh weight, and dry weight. Plant height, leaf area, number of leaves
per plant, plant density, fresh weight, and dry weight showed highly significant differences (P = 0.01)
among the four cultivars while stem diameter showed significant differences (P = 0.05). Number of days
to 50% flowering showed insignificant differences among the four cultivars. Mabrook exceeded all the
other cultivars in plant height, fresh weight, and dry weight. Mabrook and Abu Sabein Kambal exceeded
the others in leaf number, leaf area, and plant density.
Key words: Abu Sabein Kambal, Abu Sabein, Mabrook, Panar 888
INTRODUCTION
The effort on sorghum breeding in the Sudan
has been directed toward the development of
high-yielding types suitable for mechanical
harvesting.[1,2]
Due to the increasing demand for
animal products and the establishment of dairy
farms and animal fattening centers, some efforts
were deviated toward the production of forage
sorghum varieties.
The animal wealth in the Sudan depends mainly
on natural pastures and the animal owners adopt
nomadic system. In the Sudan, the irrigated
forages constitute only 4% of the total available
forage and they were concentrated in north and
central Sudan.[3]
Forage crops of the genus Sorghum were classified
intothreecategories:Theforagesorghum(Sorghum
bicolor L. Moench), the forage of Sudan grass
(Sorghum sudanensis L.), and the sorghum Sudan
grass hybrids. The forage sorghum included dual-
purpose cultivars that can be used for grains and
forage production, for example, Mugud Hemaisy,
Abu Sabein Kambal, and Abu Sabein Aliab.[4]
Sudan grass, the cultivar Garawia, constitutes most
of the grass forage in the Sudan.[5]
It was proved that
Garawia had better nutritive value fast regrowth
after the first cut than Abu Sabein due to larger
number of leaves per plant and slender stem.[6]
I
was also found that Sudan grass more productive in
the second cut, less affected by soil salinity, more
resistant to stem borer and one of the best pollinator
sorghum cultivars for production of F1
hybrids.,[7]
There were many sorghum-Sudan grass hybrids
approved in the Sudan. These included Pioneer
988 and Pioneer 877 which were sorghum-
Sudan grass interspecific hybrids from Pioneer
International Seed Company[8]
than Jumbo
which was released by the Agricultural Research
Corporation, Wad Medani, Sudan.[9]
Panar 888
was a sorghum-Sudan grass hybrid from Pacific
Seed Company.[6]
Sorghum-Sudan grass hybrids
were reported to be more responsive to recurrent
cutting compared to Abu Sabein (El Hamada
Address for correspondence:
Ahmed Abdel Aziz Hamid Abdel Aziz,
E-mail: azhamid1952@gmail.com
Elamin and Aziz: Assessment of Two Forage Sorghum
AEXTJ/Jan-Mar-2019/Vol 3/Issue 1 36
et al., 2003). Furthermore, the hybrids were more
productive during winter months than Abu Sabein
and Gadam El Hamam.[6]
The hybrids were found to be more responsive to
recurrent cutting and can grow more better than
Abu Sabein during the winter months.[10]
The objectives of this study were to compare the
growth and yield components of two introduced
forage interspecific hybrids with two local checks,
Abu Sabein local and Abu Sabein Kambal.
MATERIALS AND METHODS
A trial was conducted in the experimental farm of
the Faculty of Agriculture and Neutral Resources,
University of Bakht Al Ruda, Ed Dueim, Sudan,
for two successive Kharif seasons 2011 and 2012.
The planted material consisted of four forage
grasses, namely Abu Sabein and Abu Sabein
Kambal (S. bicolor L. Moench), Panar 888, and
Mabrouk. The last two were sorghum-Sudan grass
(Sorghum sudanense) interspecific hybrids. Seeds
were supplied by Shambat Research Station.
Treatments were arranged in a randomized
complete block design with four replicates.
Land was prepared by deep plowing, harrowing,
leveling, and ridging. The experimental area was
divided into 16 plots, each plot with five ridges
of 5 m long and 0.75 m apart. Seeds were planted
in channels at the side of the ridge at seed rate of
20 kg/Feddan according to Ishag (1989). Weeding
was done manually at 2 weeks after sowing.
Nitrogen fertilizer was applied as urea (urea = 46%
nitrogen) at the rate of 80 kg/Feddan. Irrigation
water was applied at intervals of 10–15 days. The
trial received seven irrigations during the growing
period and the area received few rain showers.
A sample of 10 plants randomly selected from
the central ridges of each plot and tagged for
data collection. Plant height was recorded from
the tagged plants using a metric ruler from
the lowest node above the soil surface to the top
of the plant. The stem diameter was measured
from the sampled or the tagged plants using a
Vernea. The number of days to 50% flowering
was recorded for each plot when about half of
the plants of each plot started to shed pollens.
Number of leaves per plant was counted for each
tagged plant and the average of the 10 plants was
calculated. Leaf area was obtained by multiplying
the length by the maximum width for the fourth
leaf by the factor 0.77.
Analysis of variance for randomized complete
block design was applied according to Gomez and
Gomez[11]
and the least significant difference was
used to compare the means.
RESULTS
Means for the different characters of the two
seasons are presented in Table 1. Analysis of
variance showed that there were significant
differences among the four cultivars in plant
height, number of leaves per plant, leaf area, plant
density green matter yield, and dry matter yield,
at (P = 0.01) level of significance. Stem diameter
showed significant differences among the cultivars
at (P = 0.05) the level of significance. Number
of days to 50% flowering showed no significant
differences among the four cultivars [Table 2].
In plant height, Mabrouk exceeded significantly
all the other three cultivars while Abu Sabein
Kambal exceeded significantly both Abu Sabein
Local and Panar 888.
Panar 888 was exceeded significantly by the other
three cultivars in number of leaves per plant and
stem diameter, while these three cultivars showed
no significant differences among them.
Results also showed that Mabrouk exceeded
significantly Abu Sabein Local and Panar 888 and
Table 1: Mean of the different characters, the average of two seasons 2011 and 2012
Genotype Plant
height
Leaf
number
Stem
diameter
Leaf
area
Days to 50%
flowering
Plant
density
Fresh
weight
Dry
weight
Mabrouk 218.5a
10.6a
0.9a
235.1a
65.4a
170.6a
51.5a
13.1a
Abu Sabein 183.5b
9.9a
0.8a
231.8ab
69.9a
161.8a
41.8b
10.0b
Abu Sabein Kambal 174.3c
9.6a
0.8a
210.9b
64.5a
141.3b
37.8bc
8.1bc
Panar 888 173.5c
7.8b
0.6b
170.8c
63.4a
136.8b
33.0c
8.1bc
Mean 187.3 9.4 0.8 212.2 64.5 152.6 41.0 10.0
C.V. 5.3 16.4 17.6 10.4 4.9 10.2 12.7 18.6
LSD 78.9 1.9 0.02 39.4 8.0 19.2 5.9 2.8
S.E. 9.9 0.5 0.1 22.1 3.2 15.5 5.2 1.9
Means currying the same numbers are insignificantly different
Elamin and Aziz: Assessment of Two Forage Sorghum
AEXTJ/Jan-Mar-2019/Vol 3/Issue 1 37
Abu Sabein Kambal exceeded significantly, also,
Panar 888 in leaf area.
DISCUSSION
The results were obtained wide range of variation
among the four forage cultivars in the characters
studied. The highly significant differences in plant
height among the four cultivars found in this study
agreed with results obtained by Cruz et al. and
Khair.[3,12]
The highly significant variation in number of
leaves per plant observed in this study among the
four cultivars agreed with the results obtained
by Poehlman.[13]
Stem diameter showed highly
significant variation among the four cultivars
confirmed the results found by Bushara,[14]
but the
plants were higher than the plant obtained in this
study.
According to Khair (1995), drought has adverse
effect on leaf area and accordingly in forage yield.
Mohamed (1910) reported that forage yield was
dependent on plant height, number of leaves per
plant, and leaf area.
The significant variation in number of days to
50% flowering observed in this work confirmed
the results obtained.[15]
REFERENCES
1.	 Tahir WM. Sorghum improvement for mechanization in
the central rain land of the Sudan. Emp J ExpAgric 1964;
32:211-8.
2.	 Walton PD. Plant breeding in the central Rainland of
the Sudan. Emp J Exp Agric 1959;27:79-85.
3.	 Khair MA. Principles of Forage Crops Production.
Training and Publication Administration. Sudan:
Agricultural Research Corporation; 1999.
4.	 Kambal AE. Naming abu sabein sorghums. J Agric Sci
2003;11:449-52.
5.	Mohammed MI. Breeding for Forage Sorghum
(Sorghum bicolor L. Moench) in the Sudan Using Local
Stocks as Males and Introduced Stocks as Females.
Ph.D. Thesis, Faculty of Agriculture, University of K,
Sudan; 2005.
6.	 Khair MA, Babiker EA, Ibrahim BA, Mohammed BA.
Speed Feed and Jumbo, New Sorghum × Sudan Grass
HybridsforIrrigatedAreasintheCentralSudan.A Paper
Submitted to the Variety Release Committee. Wad-
Medani, Sudan: Agricultural Research Corporation;
1995.
7.	 Emundus LK, Zummo N. Sorghum Diseases in United
States and their Control. Agriculture Hand Book 468.
Washington, D.C: U.S. Department of Agriculture;
1975. p. 46.
8.	 Ishag HM. Pioneer 988 Forage Sorghum for Irrigated
Gezira. A Report Submitted to the Variety Release
Committee. ARC, Wad Medani, Sudan; 1989.
9.	 Nour AM, Khair MA, Mohammed BA. Performance
of Forage Sorghum Hybrids under Sudan Conditions.
A Paper Submitted to the Variety Release Committee.
Wad Medani, Sudan:Agricultural Research Corporation;
1998.
10.	 ElAhmadiAB,KhairMA,MohammedMI.Comparative
Performance of Safed Moti, a Grain Sorghum × Sudan
Grass Hybrid from India. A Paper Submitted to the
Variety Release Committee. ARC. Wad Medani. Sudan;
2003.
11.	Gomez KA, Gomez AA. Statistical Procedures for
Agricultural Research. New York: John Wiley and
Sons; 1984. p. 680.
12.	 Cruz R, Garcia M, Ayala JR. Evaluation and selection
of forage sorghum varieties, morphology cal and
productive traits. Cuban J Agric Sci 1995;29:233-8.
13.	 Poehlman JM. Breeding Field Crops. 3rd
 ed. New York:
Van Nostrand Reinhold Co Ltd.; 1987. p. 508-55.
14.	Bushara MA. Tester Analysis for Hetrosis and
Combining Ability in some Genotypes of Grain
Sorghum (Sorghum bicolor L. Moensch) MSc Thesis,
Faculty of Agriculture, University of Khartoum; 1999.
15.	 Quinby JR. Sorghum Improvement and the Genetics of
Growth College Station. College Station, TX: Texas A
and M University Press; 1974. p. 108.
Table 2: Means of the different characters for the four cultivars, the average of two seasons 2011 and 2012
Source of
variation
Degrees of
freedom
Plant
height
Leaf
number
Stem
diameter
Leaf
area
Days to 50%
flowering
Plant
density
Fresh
weight
Dry
weight
Block 3 31.76 3.03 2.02 272.04 17.20 439.28 61.20 3.20
Genotype 3 3652.81** 11.72** 0.08** 7006.22** 32.78 2103.12** 494.30** 38.78**
Season 1 4023.05** 13.78* 0.03 30830.17** 457.53** 2476.50 4.50 0.03
Genotype/Season 3 235.23 1.04 0.07 2435.66** 27.45 1018.20 11.50 1.95
Error 21 98.60 2.34 0.02 488.17 9.95 240.02 27.30 3.49
Fcalculated
37.05 5.09 4.0 14.4 1.35 8.8 18.11 11.11
FTable
 (5%) 2.70 2.70 2.70 2.70 2.70 2.70 2.70 2.70
FTable
 (1%) 3.80 3.80 3.80 3.80 3.80 3.80 3.80 3.80
**Highly significant differences, *Significant differences

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Assessment of Two Forage Sorghum-Sudan Grass Hybrids#

  • 1. © 2019, AEXTJ. All Rights Reserved 35 Available Online at www.aextj.com Agricultural Extension Journal 2019; 3(1):35-37 ISSN 2521 – 0408 RESEARCH ARTICLE Assessment of Two Forage Sorghum-Sudan Grass Hybrids# Gamar Alanbiaa Hag Musa Elamin1 , Ahmed Abdel Aziz Hamid Abdel Aziz2 1 Department of Crop Science, Faculty of Agriculture and Natural Resources, University of Bakht Al Ruda, Ed Dueim, Sudan, 2 Department of Agronomy and Horticulture, Faculty of Agricultural Technology and Fish Sciences, Al Neelain University, Khartoum, Sudan # Part of MSc thesis submitted by the first author Received: 15-10-2018; Revised: 15-11-2018; Accepted: 01-02-2019 ABSTRACT This trial was conducted for two consecutive Kharif seasons of 2011 and 2012 to compare two interspecific hybrids of sorghum and Sudan grass, namely Mabrook and Panar 888 with two local checks Abu Sabein andAbu Sabein Kambal. Cultivars were sown in a randomized complete block design with four replicates. Data were collected on plant height, number of leaves per plant, leaf area, stem diameter, number of days to 50 % flowering, plant density, fresh weight, and dry weight. Plant height, leaf area, number of leaves per plant, plant density, fresh weight, and dry weight showed highly significant differences (P = 0.01) among the four cultivars while stem diameter showed significant differences (P = 0.05). Number of days to 50% flowering showed insignificant differences among the four cultivars. Mabrook exceeded all the other cultivars in plant height, fresh weight, and dry weight. Mabrook and Abu Sabein Kambal exceeded the others in leaf number, leaf area, and plant density. Key words: Abu Sabein Kambal, Abu Sabein, Mabrook, Panar 888 INTRODUCTION The effort on sorghum breeding in the Sudan has been directed toward the development of high-yielding types suitable for mechanical harvesting.[1,2] Due to the increasing demand for animal products and the establishment of dairy farms and animal fattening centers, some efforts were deviated toward the production of forage sorghum varieties. The animal wealth in the Sudan depends mainly on natural pastures and the animal owners adopt nomadic system. In the Sudan, the irrigated forages constitute only 4% of the total available forage and they were concentrated in north and central Sudan.[3] Forage crops of the genus Sorghum were classified intothreecategories:Theforagesorghum(Sorghum bicolor L. Moench), the forage of Sudan grass (Sorghum sudanensis L.), and the sorghum Sudan grass hybrids. The forage sorghum included dual- purpose cultivars that can be used for grains and forage production, for example, Mugud Hemaisy, Abu Sabein Kambal, and Abu Sabein Aliab.[4] Sudan grass, the cultivar Garawia, constitutes most of the grass forage in the Sudan.[5] It was proved that Garawia had better nutritive value fast regrowth after the first cut than Abu Sabein due to larger number of leaves per plant and slender stem.[6] I was also found that Sudan grass more productive in the second cut, less affected by soil salinity, more resistant to stem borer and one of the best pollinator sorghum cultivars for production of F1 hybrids.,[7] There were many sorghum-Sudan grass hybrids approved in the Sudan. These included Pioneer 988 and Pioneer 877 which were sorghum- Sudan grass interspecific hybrids from Pioneer International Seed Company[8] than Jumbo which was released by the Agricultural Research Corporation, Wad Medani, Sudan.[9] Panar 888 was a sorghum-Sudan grass hybrid from Pacific Seed Company.[6] Sorghum-Sudan grass hybrids were reported to be more responsive to recurrent cutting compared to Abu Sabein (El Hamada Address for correspondence: Ahmed Abdel Aziz Hamid Abdel Aziz, E-mail: azhamid1952@gmail.com
  • 2. Elamin and Aziz: Assessment of Two Forage Sorghum AEXTJ/Jan-Mar-2019/Vol 3/Issue 1 36 et al., 2003). Furthermore, the hybrids were more productive during winter months than Abu Sabein and Gadam El Hamam.[6] The hybrids were found to be more responsive to recurrent cutting and can grow more better than Abu Sabein during the winter months.[10] The objectives of this study were to compare the growth and yield components of two introduced forage interspecific hybrids with two local checks, Abu Sabein local and Abu Sabein Kambal. MATERIALS AND METHODS A trial was conducted in the experimental farm of the Faculty of Agriculture and Neutral Resources, University of Bakht Al Ruda, Ed Dueim, Sudan, for two successive Kharif seasons 2011 and 2012. The planted material consisted of four forage grasses, namely Abu Sabein and Abu Sabein Kambal (S. bicolor L. Moench), Panar 888, and Mabrouk. The last two were sorghum-Sudan grass (Sorghum sudanense) interspecific hybrids. Seeds were supplied by Shambat Research Station. Treatments were arranged in a randomized complete block design with four replicates. Land was prepared by deep plowing, harrowing, leveling, and ridging. The experimental area was divided into 16 plots, each plot with five ridges of 5 m long and 0.75 m apart. Seeds were planted in channels at the side of the ridge at seed rate of 20 kg/Feddan according to Ishag (1989). Weeding was done manually at 2 weeks after sowing. Nitrogen fertilizer was applied as urea (urea = 46% nitrogen) at the rate of 80 kg/Feddan. Irrigation water was applied at intervals of 10–15 days. The trial received seven irrigations during the growing period and the area received few rain showers. A sample of 10 plants randomly selected from the central ridges of each plot and tagged for data collection. Plant height was recorded from the tagged plants using a metric ruler from the lowest node above the soil surface to the top of the plant. The stem diameter was measured from the sampled or the tagged plants using a Vernea. The number of days to 50% flowering was recorded for each plot when about half of the plants of each plot started to shed pollens. Number of leaves per plant was counted for each tagged plant and the average of the 10 plants was calculated. Leaf area was obtained by multiplying the length by the maximum width for the fourth leaf by the factor 0.77. Analysis of variance for randomized complete block design was applied according to Gomez and Gomez[11] and the least significant difference was used to compare the means. RESULTS Means for the different characters of the two seasons are presented in Table 1. Analysis of variance showed that there were significant differences among the four cultivars in plant height, number of leaves per plant, leaf area, plant density green matter yield, and dry matter yield, at (P = 0.01) level of significance. Stem diameter showed significant differences among the cultivars at (P = 0.05) the level of significance. Number of days to 50% flowering showed no significant differences among the four cultivars [Table 2]. In plant height, Mabrouk exceeded significantly all the other three cultivars while Abu Sabein Kambal exceeded significantly both Abu Sabein Local and Panar 888. Panar 888 was exceeded significantly by the other three cultivars in number of leaves per plant and stem diameter, while these three cultivars showed no significant differences among them. Results also showed that Mabrouk exceeded significantly Abu Sabein Local and Panar 888 and Table 1: Mean of the different characters, the average of two seasons 2011 and 2012 Genotype Plant height Leaf number Stem diameter Leaf area Days to 50% flowering Plant density Fresh weight Dry weight Mabrouk 218.5a 10.6a 0.9a 235.1a 65.4a 170.6a 51.5a 13.1a Abu Sabein 183.5b 9.9a 0.8a 231.8ab 69.9a 161.8a 41.8b 10.0b Abu Sabein Kambal 174.3c 9.6a 0.8a 210.9b 64.5a 141.3b 37.8bc 8.1bc Panar 888 173.5c 7.8b 0.6b 170.8c 63.4a 136.8b 33.0c 8.1bc Mean 187.3 9.4 0.8 212.2 64.5 152.6 41.0 10.0 C.V. 5.3 16.4 17.6 10.4 4.9 10.2 12.7 18.6 LSD 78.9 1.9 0.02 39.4 8.0 19.2 5.9 2.8 S.E. 9.9 0.5 0.1 22.1 3.2 15.5 5.2 1.9 Means currying the same numbers are insignificantly different
  • 3. Elamin and Aziz: Assessment of Two Forage Sorghum AEXTJ/Jan-Mar-2019/Vol 3/Issue 1 37 Abu Sabein Kambal exceeded significantly, also, Panar 888 in leaf area. DISCUSSION The results were obtained wide range of variation among the four forage cultivars in the characters studied. The highly significant differences in plant height among the four cultivars found in this study agreed with results obtained by Cruz et al. and Khair.[3,12] The highly significant variation in number of leaves per plant observed in this study among the four cultivars agreed with the results obtained by Poehlman.[13] Stem diameter showed highly significant variation among the four cultivars confirmed the results found by Bushara,[14] but the plants were higher than the plant obtained in this study. According to Khair (1995), drought has adverse effect on leaf area and accordingly in forage yield. Mohamed (1910) reported that forage yield was dependent on plant height, number of leaves per plant, and leaf area. The significant variation in number of days to 50% flowering observed in this work confirmed the results obtained.[15] REFERENCES 1. Tahir WM. Sorghum improvement for mechanization in the central rain land of the Sudan. Emp J ExpAgric 1964; 32:211-8. 2. Walton PD. Plant breeding in the central Rainland of the Sudan. Emp J Exp Agric 1959;27:79-85. 3. Khair MA. Principles of Forage Crops Production. Training and Publication Administration. Sudan: Agricultural Research Corporation; 1999. 4. Kambal AE. Naming abu sabein sorghums. J Agric Sci 2003;11:449-52. 5. Mohammed MI. Breeding for Forage Sorghum (Sorghum bicolor L. Moench) in the Sudan Using Local Stocks as Males and Introduced Stocks as Females. Ph.D. Thesis, Faculty of Agriculture, University of K, Sudan; 2005. 6. Khair MA, Babiker EA, Ibrahim BA, Mohammed BA. Speed Feed and Jumbo, New Sorghum × Sudan Grass HybridsforIrrigatedAreasintheCentralSudan.A Paper Submitted to the Variety Release Committee. Wad- Medani, Sudan: Agricultural Research Corporation; 1995. 7. Emundus LK, Zummo N. Sorghum Diseases in United States and their Control. Agriculture Hand Book 468. Washington, D.C: U.S. Department of Agriculture; 1975. p. 46. 8. Ishag HM. Pioneer 988 Forage Sorghum for Irrigated Gezira. A Report Submitted to the Variety Release Committee. ARC, Wad Medani, Sudan; 1989. 9. Nour AM, Khair MA, Mohammed BA. Performance of Forage Sorghum Hybrids under Sudan Conditions. A Paper Submitted to the Variety Release Committee. Wad Medani, Sudan:Agricultural Research Corporation; 1998. 10. ElAhmadiAB,KhairMA,MohammedMI.Comparative Performance of Safed Moti, a Grain Sorghum × Sudan Grass Hybrid from India. A Paper Submitted to the Variety Release Committee. ARC. Wad Medani. Sudan; 2003. 11. Gomez KA, Gomez AA. Statistical Procedures for Agricultural Research. New York: John Wiley and Sons; 1984. p. 680. 12. Cruz R, Garcia M, Ayala JR. Evaluation and selection of forage sorghum varieties, morphology cal and productive traits. Cuban J Agric Sci 1995;29:233-8. 13. Poehlman JM. Breeding Field Crops. 3rd  ed. New York: Van Nostrand Reinhold Co Ltd.; 1987. p. 508-55. 14. Bushara MA. Tester Analysis for Hetrosis and Combining Ability in some Genotypes of Grain Sorghum (Sorghum bicolor L. Moensch) MSc Thesis, Faculty of Agriculture, University of Khartoum; 1999. 15. Quinby JR. Sorghum Improvement and the Genetics of Growth College Station. College Station, TX: Texas A and M University Press; 1974. p. 108. Table 2: Means of the different characters for the four cultivars, the average of two seasons 2011 and 2012 Source of variation Degrees of freedom Plant height Leaf number Stem diameter Leaf area Days to 50% flowering Plant density Fresh weight Dry weight Block 3 31.76 3.03 2.02 272.04 17.20 439.28 61.20 3.20 Genotype 3 3652.81** 11.72** 0.08** 7006.22** 32.78 2103.12** 494.30** 38.78** Season 1 4023.05** 13.78* 0.03 30830.17** 457.53** 2476.50 4.50 0.03 Genotype/Season 3 235.23 1.04 0.07 2435.66** 27.45 1018.20 11.50 1.95 Error 21 98.60 2.34 0.02 488.17 9.95 240.02 27.30 3.49 Fcalculated 37.05 5.09 4.0 14.4 1.35 8.8 18.11 11.11 FTable  (5%) 2.70 2.70 2.70 2.70 2.70 2.70 2.70 2.70 FTable  (1%) 3.80 3.80 3.80 3.80 3.80 3.80 3.80 3.80 **Highly significant differences, *Significant differences