SlideShare ist ein Scribd-Unternehmen logo
1 von 4
ENZYMES PROKAROTES EUKAYOTES
Initiator
Dna A --
Helicase Dna B MCM
Ligase-Loader Dna C --
SSB SSB Replication Protein A
Primer Dna G DNA Poly ᾀ/Primase
RNAse DNA Pol - I RNAse H/Fan 1(last nucleotide)
Gap filler DNA Pol - I DNA Poly ᾀ
Ligase DNA Ligase DNA Ligase
Methylator Dam Methyltransferase(GATC)/Seq A Dam Methyltransferase(GATC)/Seq A
Topoisomerase DNA Gyrase/Top- I DNA Gyrase/Top- I,II
Terminator Tus(Ter)1234 --
DNAPolymerases
NAME Function NAME Function
DNA Pol –I 5/
-3/
3/
-5/ RNAse, Gap filling,
DNA repair
DNA Pol – ᾀ, No -
Priming &
Gap-filling
DNA Pol –II No 3/
-5/
Translesion DNA Pol – β
No - Base Excision repair
DNA Pol –III No 3/
-5/
Replication
{ɵ(E-coli)/Rolling-
circle(Bacteriopgase)}
DNA Pol – Ɣ
No
3/
-5/
Mitochondrial DNA
Replication
(Displacement/D Replication)
DNA Pol –VI - -
DNA repair &
Translesion DNA Pol –d
No
3/
-5/ Replication
DNA Pol –V - -
DNA repair &
Translesion
DNA Pol – ɛ
No
3/
-5/
--
Lagging strand is 1000-2000 bp Lagging strand is 100-200 bp
No Telomere
‘Telomerase’ is needed to end the Telomere
Replication
Multirepliconic Monorepliconic
OriC = 9mer + 13 mer(Starting site) Hemimethylation - Seq A(Starting site)
ENZYMES PROKARYOTES EUKARYOTES
RNAPolymerases Sub
Units
Function Types Product
Transcription
Factor
Function
α
Assembly of core enzyme,
Recognition of promoter
(up element)
RNAPol-I
5.8s, 18s
& 28s
rRNA
UBF(Upstream
Binding factor)
Binds Upstream Control
Element
β Catalytic centre SLI(Selectivity Factor 1) Stabilizes UBF binding
β/ Catalytic centre
RNAPol-II mRNA
most of
the
sn RNA
LINE
miRNA
TFII A
Inhibits the binding of TFII D
inhibitor
w Assembly of RNA polymerase TFII B
Additional factor helps retain
TFIIA+TFIID
σ
Recognition of
promoter(Core promoter) &
initiation
TFII C Not known properly
TFII D
(TBP + TAF)
TBP(TATA
binding protein)
Recognize TATA box
TAF(TBP
associated factor)
Recognition of Core
promoter & Regulation of
TBP
TFII E Recruite TFII H
TFII F Recruit RNA pol II
TFII H
Helicase that create
“Open complex” &
Phosphorylation of the C-
terminal domain of largest
subunit of RNA pol II
TFII i Additional factor for
initiationTFII J
RNAPol-III
tRNA
5s rRNA
U6 snRNA
H1 RNA, MRP RNA
7S L scRNA,
7S K scRNA
SINEtranscripts
TFIII A Binds with C box
TFIII B Binds with A and B box
TBP BRF B//
TFIII C Give Direction to The
TFIIIB
Rho(P)
Works for termination in ‘Rho
dependent termination’
process
--
-35 box -10 box +1
Starting point
-180 -100 -45 +1 +20
Table 2 Eukaryotic rRNA gene(RNA Pol I)
GC box CAATbox BRE TATA +1 MTE DPE
Intr
Table 3 Eukaryotic mRNA gene(RNA Pol II)
+1 +50 +70 +80 +90
Table 3 Eukaryotic 5s rRNA gene(RNAPol III)
Box
A
Box B
+1 +10 +30 +50 +70
Table 3 Eukaryotic tRNA gene(RNA Pol III)
DSE PSE TATA
+60 +30 +1
Table 3 Eukaryotic U6 snRNA gene(RNA Pol III)
Table 1 Prokaryotic Gene
UCE Core
Element
GC CAAT BRE TATA MTE DPE
Box
A
Box C
PROKARYOTES EUKARYOTES FUNCTION
Initiation
eIF3, eIF4c
eIF6, eIF4B
eIF4F
Bind to ribosome subunit
IF 1 , IF 3 Bind to mRNA
Initiator tRNA delivery
IF 2
eIF2B eIF2
eIF5
Displacement of other factor
Elongation
eEF1a Aminoacyl tRNA delivery
EF-Tu
EF-Ts eEFβƳ Recycling of EF-Tu/eEF1a
EF-g eEF2 Translocation
Termination
eRF Polypeptide Chain release
RF1, RF2,RF3
Ribosome Binding site(RBS) Scaning of 5/
methylated cap
ISER(Poliovirus)
Cap Snitching(Influenza)
Shine-Dalgarno sequence KOzak Sequence
AGGAGGU G/ACCAUGG

Weitere ähnliche Inhalte

Ähnlich wie Enzymes In Transcription, Translation and Replication

Prokaryotic transcription
Prokaryotic transcription Prokaryotic transcription
Prokaryotic transcription Sr.College
 
Transcription of eukaryotic gene
Transcription of eukaryotic geneTranscription of eukaryotic gene
Transcription of eukaryotic geneAngsumanDas2
 
3B.Transcription.pptx
3B.Transcription.pptx3B.Transcription.pptx
3B.Transcription.pptxTeshaleTekle1
 
Transcripion
Transcripion Transcripion
Transcripion aliagr
 
Labtalk 101210 optimising fret assay_den_optimising r_rel 1 production_heat s...
Labtalk 101210 optimising fret assay_den_optimising r_rel 1 production_heat s...Labtalk 101210 optimising fret assay_den_optimising r_rel 1 production_heat s...
Labtalk 101210 optimising fret assay_den_optimising r_rel 1 production_heat s...Laurence Dawkins-Hall
 
Transcription dna2011
Transcription dna2011Transcription dna2011
Transcription dna2011MUBOSScz
 
MOLECULAR GENETICS : PROKARYOTIC TRANSCRIPTION OR RNA SYNTHESIS BY DNA DEPEN...
 MOLECULAR GENETICS : PROKARYOTIC TRANSCRIPTION OR RNA SYNTHESIS BY DNA DEPEN... MOLECULAR GENETICS : PROKARYOTIC TRANSCRIPTION OR RNA SYNTHESIS BY DNA DEPEN...
MOLECULAR GENETICS : PROKARYOTIC TRANSCRIPTION OR RNA SYNTHESIS BY DNA DEPEN...Amritha S R
 
Transcription and translation
Transcription and translationTranscription and translation
Transcription and translationBlaschke's Class
 
Transcription
TranscriptionTranscription
Transcriptionjoyjulie
 
Biochemistry transcription (RNA biosynsthesis)
Biochemistry transcription (RNA biosynsthesis)Biochemistry transcription (RNA biosynsthesis)
Biochemistry transcription (RNA biosynsthesis)Prabesh Raj Jamkatel
 
Transcription (term paper) by Ganesh M
Transcription (term paper) by Ganesh MTranscription (term paper) by Ganesh M
Transcription (term paper) by Ganesh M9494458298
 

Ähnlich wie Enzymes In Transcription, Translation and Replication (20)

Prokaryotic transcription
Prokaryotic transcription Prokaryotic transcription
Prokaryotic transcription
 
Transcription of eukaryotic gene
Transcription of eukaryotic geneTranscription of eukaryotic gene
Transcription of eukaryotic gene
 
Transcription
TranscriptionTranscription
Transcription
 
Honors ~ DNA 1011
Honors ~ DNA 1011Honors ~ DNA 1011
Honors ~ DNA 1011
 
Presentation.pptx
Presentation.pptxPresentation.pptx
Presentation.pptx
 
3B.Transcription.pptx
3B.Transcription.pptx3B.Transcription.pptx
3B.Transcription.pptx
 
Transcription, mechanism
Transcription, mechanismTranscription, mechanism
Transcription, mechanism
 
Transcripion
Transcripion Transcripion
Transcripion
 
Biology ~ DNA 1011
Biology ~ DNA 1011Biology ~ DNA 1011
Biology ~ DNA 1011
 
Rna polymerase
Rna polymeraseRna polymerase
Rna polymerase
 
Labtalk 101210 optimising fret assay_den_optimising r_rel 1 production_heat s...
Labtalk 101210 optimising fret assay_den_optimising r_rel 1 production_heat s...Labtalk 101210 optimising fret assay_den_optimising r_rel 1 production_heat s...
Labtalk 101210 optimising fret assay_den_optimising r_rel 1 production_heat s...
 
Transcription dna2011
Transcription dna2011Transcription dna2011
Transcription dna2011
 
MOLECULAR GENETICS : PROKARYOTIC TRANSCRIPTION OR RNA SYNTHESIS BY DNA DEPEN...
 MOLECULAR GENETICS : PROKARYOTIC TRANSCRIPTION OR RNA SYNTHESIS BY DNA DEPEN... MOLECULAR GENETICS : PROKARYOTIC TRANSCRIPTION OR RNA SYNTHESIS BY DNA DEPEN...
MOLECULAR GENETICS : PROKARYOTIC TRANSCRIPTION OR RNA SYNTHESIS BY DNA DEPEN...
 
Transcription and translation
Transcription and translationTranscription and translation
Transcription and translation
 
Transcription
TranscriptionTranscription
Transcription
 
Hoofdstuk 17 2008 deel 2
Hoofdstuk 17 2008 deel 2Hoofdstuk 17 2008 deel 2
Hoofdstuk 17 2008 deel 2
 
Transcription NEW
Transcription NEWTranscription NEW
Transcription NEW
 
Transcription
TranscriptionTranscription
Transcription
 
Biochemistry transcription (RNA biosynsthesis)
Biochemistry transcription (RNA biosynsthesis)Biochemistry transcription (RNA biosynsthesis)
Biochemistry transcription (RNA biosynsthesis)
 
Transcription (term paper) by Ganesh M
Transcription (term paper) by Ganesh MTranscription (term paper) by Ganesh M
Transcription (term paper) by Ganesh M
 

Kürzlich hochgeladen

300003-World Science Day For Peace And Development.pptx
300003-World Science Day For Peace And Development.pptx300003-World Science Day For Peace And Development.pptx
300003-World Science Day For Peace And Development.pptxryanrooker
 
TransientOffsetin14CAftertheCarringtonEventRecordedbyPolarTreeRings
TransientOffsetin14CAftertheCarringtonEventRecordedbyPolarTreeRingsTransientOffsetin14CAftertheCarringtonEventRecordedbyPolarTreeRings
TransientOffsetin14CAftertheCarringtonEventRecordedbyPolarTreeRingsSérgio Sacani
 
Atp synthase , Atp synthase complex 1 to 4.
Atp synthase , Atp synthase complex 1 to 4.Atp synthase , Atp synthase complex 1 to 4.
Atp synthase , Atp synthase complex 1 to 4.Silpa
 
CURRENT SCENARIO OF POULTRY PRODUCTION IN INDIA
CURRENT SCENARIO OF POULTRY PRODUCTION IN INDIACURRENT SCENARIO OF POULTRY PRODUCTION IN INDIA
CURRENT SCENARIO OF POULTRY PRODUCTION IN INDIADr. TATHAGAT KHOBRAGADE
 
LUNULARIA -features, morphology, anatomy ,reproduction etc.
LUNULARIA -features, morphology, anatomy ,reproduction etc.LUNULARIA -features, morphology, anatomy ,reproduction etc.
LUNULARIA -features, morphology, anatomy ,reproduction etc.Silpa
 
Zoology 5th semester notes( Sumit_yadav).pdf
Zoology 5th semester notes( Sumit_yadav).pdfZoology 5th semester notes( Sumit_yadav).pdf
Zoology 5th semester notes( Sumit_yadav).pdfSumit Kumar yadav
 
development of diagnostic enzyme assay to detect leuser virus
development of diagnostic enzyme assay to detect leuser virusdevelopment of diagnostic enzyme assay to detect leuser virus
development of diagnostic enzyme assay to detect leuser virusNazaninKarimi6
 
Digital Dentistry.Digital Dentistryvv.pptx
Digital Dentistry.Digital Dentistryvv.pptxDigital Dentistry.Digital Dentistryvv.pptx
Digital Dentistry.Digital Dentistryvv.pptxMohamedFarag457087
 
Role of AI in seed science Predictive modelling and Beyond.pptx
Role of AI in seed science  Predictive modelling and  Beyond.pptxRole of AI in seed science  Predictive modelling and  Beyond.pptx
Role of AI in seed science Predictive modelling and Beyond.pptxArvind Kumar
 
Grade 7 - Lesson 1 - Microscope and Its Functions
Grade 7 - Lesson 1 - Microscope and Its FunctionsGrade 7 - Lesson 1 - Microscope and Its Functions
Grade 7 - Lesson 1 - Microscope and Its FunctionsOrtegaSyrineMay
 
Call Girls Ahmedabad +917728919243 call me Independent Escort Service
Call Girls Ahmedabad +917728919243 call me Independent Escort ServiceCall Girls Ahmedabad +917728919243 call me Independent Escort Service
Call Girls Ahmedabad +917728919243 call me Independent Escort Serviceshivanisharma5244
 
GBSN - Microbiology (Unit 3)Defense Mechanism of the body
GBSN - Microbiology (Unit 3)Defense Mechanism of the body GBSN - Microbiology (Unit 3)Defense Mechanism of the body
GBSN - Microbiology (Unit 3)Defense Mechanism of the body Areesha Ahmad
 
PSYCHOSOCIAL NEEDS. in nursing II sem pptx
PSYCHOSOCIAL NEEDS. in nursing II sem pptxPSYCHOSOCIAL NEEDS. in nursing II sem pptx
PSYCHOSOCIAL NEEDS. in nursing II sem pptxSuji236384
 
Proteomics: types, protein profiling steps etc.
Proteomics: types, protein profiling steps etc.Proteomics: types, protein profiling steps etc.
Proteomics: types, protein profiling steps etc.Silpa
 
Dr. E. Muralinath_ Blood indices_clinical aspects
Dr. E. Muralinath_ Blood indices_clinical  aspectsDr. E. Muralinath_ Blood indices_clinical  aspects
Dr. E. Muralinath_ Blood indices_clinical aspectsmuralinath2
 
Thyroid Physiology_Dr.E. Muralinath_ Associate Professor
Thyroid Physiology_Dr.E. Muralinath_ Associate ProfessorThyroid Physiology_Dr.E. Muralinath_ Associate Professor
Thyroid Physiology_Dr.E. Muralinath_ Associate Professormuralinath2
 
module for grade 9 for distance learning
module for grade 9 for distance learningmodule for grade 9 for distance learning
module for grade 9 for distance learninglevieagacer
 

Kürzlich hochgeladen (20)

300003-World Science Day For Peace And Development.pptx
300003-World Science Day For Peace And Development.pptx300003-World Science Day For Peace And Development.pptx
300003-World Science Day For Peace And Development.pptx
 
TransientOffsetin14CAftertheCarringtonEventRecordedbyPolarTreeRings
TransientOffsetin14CAftertheCarringtonEventRecordedbyPolarTreeRingsTransientOffsetin14CAftertheCarringtonEventRecordedbyPolarTreeRings
TransientOffsetin14CAftertheCarringtonEventRecordedbyPolarTreeRings
 
Atp synthase , Atp synthase complex 1 to 4.
Atp synthase , Atp synthase complex 1 to 4.Atp synthase , Atp synthase complex 1 to 4.
Atp synthase , Atp synthase complex 1 to 4.
 
CURRENT SCENARIO OF POULTRY PRODUCTION IN INDIA
CURRENT SCENARIO OF POULTRY PRODUCTION IN INDIACURRENT SCENARIO OF POULTRY PRODUCTION IN INDIA
CURRENT SCENARIO OF POULTRY PRODUCTION IN INDIA
 
Site Acceptance Test .
Site Acceptance Test                    .Site Acceptance Test                    .
Site Acceptance Test .
 
LUNULARIA -features, morphology, anatomy ,reproduction etc.
LUNULARIA -features, morphology, anatomy ,reproduction etc.LUNULARIA -features, morphology, anatomy ,reproduction etc.
LUNULARIA -features, morphology, anatomy ,reproduction etc.
 
Zoology 5th semester notes( Sumit_yadav).pdf
Zoology 5th semester notes( Sumit_yadav).pdfZoology 5th semester notes( Sumit_yadav).pdf
Zoology 5th semester notes( Sumit_yadav).pdf
 
development of diagnostic enzyme assay to detect leuser virus
development of diagnostic enzyme assay to detect leuser virusdevelopment of diagnostic enzyme assay to detect leuser virus
development of diagnostic enzyme assay to detect leuser virus
 
Digital Dentistry.Digital Dentistryvv.pptx
Digital Dentistry.Digital Dentistryvv.pptxDigital Dentistry.Digital Dentistryvv.pptx
Digital Dentistry.Digital Dentistryvv.pptx
 
Role of AI in seed science Predictive modelling and Beyond.pptx
Role of AI in seed science  Predictive modelling and  Beyond.pptxRole of AI in seed science  Predictive modelling and  Beyond.pptx
Role of AI in seed science Predictive modelling and Beyond.pptx
 
Grade 7 - Lesson 1 - Microscope and Its Functions
Grade 7 - Lesson 1 - Microscope and Its FunctionsGrade 7 - Lesson 1 - Microscope and Its Functions
Grade 7 - Lesson 1 - Microscope and Its Functions
 
Call Girls Ahmedabad +917728919243 call me Independent Escort Service
Call Girls Ahmedabad +917728919243 call me Independent Escort ServiceCall Girls Ahmedabad +917728919243 call me Independent Escort Service
Call Girls Ahmedabad +917728919243 call me Independent Escort Service
 
GBSN - Microbiology (Unit 3)Defense Mechanism of the body
GBSN - Microbiology (Unit 3)Defense Mechanism of the body GBSN - Microbiology (Unit 3)Defense Mechanism of the body
GBSN - Microbiology (Unit 3)Defense Mechanism of the body
 
PSYCHOSOCIAL NEEDS. in nursing II sem pptx
PSYCHOSOCIAL NEEDS. in nursing II sem pptxPSYCHOSOCIAL NEEDS. in nursing II sem pptx
PSYCHOSOCIAL NEEDS. in nursing II sem pptx
 
Proteomics: types, protein profiling steps etc.
Proteomics: types, protein profiling steps etc.Proteomics: types, protein profiling steps etc.
Proteomics: types, protein profiling steps etc.
 
Dr. E. Muralinath_ Blood indices_clinical aspects
Dr. E. Muralinath_ Blood indices_clinical  aspectsDr. E. Muralinath_ Blood indices_clinical  aspects
Dr. E. Muralinath_ Blood indices_clinical aspects
 
PATNA CALL GIRLS 8617370543 LOW PRICE ESCORT SERVICE
PATNA CALL GIRLS 8617370543 LOW PRICE ESCORT SERVICEPATNA CALL GIRLS 8617370543 LOW PRICE ESCORT SERVICE
PATNA CALL GIRLS 8617370543 LOW PRICE ESCORT SERVICE
 
Thyroid Physiology_Dr.E. Muralinath_ Associate Professor
Thyroid Physiology_Dr.E. Muralinath_ Associate ProfessorThyroid Physiology_Dr.E. Muralinath_ Associate Professor
Thyroid Physiology_Dr.E. Muralinath_ Associate Professor
 
module for grade 9 for distance learning
module for grade 9 for distance learningmodule for grade 9 for distance learning
module for grade 9 for distance learning
 
Clean In Place(CIP).pptx .
Clean In Place(CIP).pptx                 .Clean In Place(CIP).pptx                 .
Clean In Place(CIP).pptx .
 

Enzymes In Transcription, Translation and Replication

  • 1. ENZYMES PROKAROTES EUKAYOTES Initiator Dna A -- Helicase Dna B MCM Ligase-Loader Dna C -- SSB SSB Replication Protein A Primer Dna G DNA Poly ᾀ/Primase RNAse DNA Pol - I RNAse H/Fan 1(last nucleotide) Gap filler DNA Pol - I DNA Poly ᾀ Ligase DNA Ligase DNA Ligase Methylator Dam Methyltransferase(GATC)/Seq A Dam Methyltransferase(GATC)/Seq A Topoisomerase DNA Gyrase/Top- I DNA Gyrase/Top- I,II Terminator Tus(Ter)1234 -- DNAPolymerases NAME Function NAME Function DNA Pol –I 5/ -3/ 3/ -5/ RNAse, Gap filling, DNA repair DNA Pol – ᾀ, No - Priming & Gap-filling DNA Pol –II No 3/ -5/ Translesion DNA Pol – β No - Base Excision repair DNA Pol –III No 3/ -5/ Replication {ɵ(E-coli)/Rolling- circle(Bacteriopgase)} DNA Pol – Ɣ No 3/ -5/ Mitochondrial DNA Replication (Displacement/D Replication) DNA Pol –VI - - DNA repair & Translesion DNA Pol –d No 3/ -5/ Replication DNA Pol –V - - DNA repair & Translesion DNA Pol – ɛ No 3/ -5/ -- Lagging strand is 1000-2000 bp Lagging strand is 100-200 bp No Telomere ‘Telomerase’ is needed to end the Telomere Replication Multirepliconic Monorepliconic OriC = 9mer + 13 mer(Starting site) Hemimethylation - Seq A(Starting site)
  • 2. ENZYMES PROKARYOTES EUKARYOTES RNAPolymerases Sub Units Function Types Product Transcription Factor Function α Assembly of core enzyme, Recognition of promoter (up element) RNAPol-I 5.8s, 18s & 28s rRNA UBF(Upstream Binding factor) Binds Upstream Control Element β Catalytic centre SLI(Selectivity Factor 1) Stabilizes UBF binding β/ Catalytic centre RNAPol-II mRNA most of the sn RNA LINE miRNA TFII A Inhibits the binding of TFII D inhibitor w Assembly of RNA polymerase TFII B Additional factor helps retain TFIIA+TFIID σ Recognition of promoter(Core promoter) & initiation TFII C Not known properly TFII D (TBP + TAF) TBP(TATA binding protein) Recognize TATA box TAF(TBP associated factor) Recognition of Core promoter & Regulation of TBP TFII E Recruite TFII H TFII F Recruit RNA pol II TFII H Helicase that create “Open complex” & Phosphorylation of the C- terminal domain of largest subunit of RNA pol II TFII i Additional factor for initiationTFII J RNAPol-III tRNA 5s rRNA U6 snRNA H1 RNA, MRP RNA 7S L scRNA, 7S K scRNA SINEtranscripts TFIII A Binds with C box TFIII B Binds with A and B box TBP BRF B// TFIII C Give Direction to The TFIIIB Rho(P) Works for termination in ‘Rho dependent termination’ process --
  • 3. -35 box -10 box +1 Starting point -180 -100 -45 +1 +20 Table 2 Eukaryotic rRNA gene(RNA Pol I) GC box CAATbox BRE TATA +1 MTE DPE Intr Table 3 Eukaryotic mRNA gene(RNA Pol II) +1 +50 +70 +80 +90 Table 3 Eukaryotic 5s rRNA gene(RNAPol III) Box A Box B +1 +10 +30 +50 +70 Table 3 Eukaryotic tRNA gene(RNA Pol III) DSE PSE TATA +60 +30 +1 Table 3 Eukaryotic U6 snRNA gene(RNA Pol III) Table 1 Prokaryotic Gene UCE Core Element GC CAAT BRE TATA MTE DPE Box A Box C
  • 4. PROKARYOTES EUKARYOTES FUNCTION Initiation eIF3, eIF4c eIF6, eIF4B eIF4F Bind to ribosome subunit IF 1 , IF 3 Bind to mRNA Initiator tRNA delivery IF 2 eIF2B eIF2 eIF5 Displacement of other factor Elongation eEF1a Aminoacyl tRNA delivery EF-Tu EF-Ts eEFβƳ Recycling of EF-Tu/eEF1a EF-g eEF2 Translocation Termination eRF Polypeptide Chain release RF1, RF2,RF3 Ribosome Binding site(RBS) Scaning of 5/ methylated cap ISER(Poliovirus) Cap Snitching(Influenza) Shine-Dalgarno sequence KOzak Sequence AGGAGGU G/ACCAUGG