Dinitro salicylic acid (DNSA) method

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Protocol

Dinitrosalicylicacid(DNSA)method

Particulars B S1 S2 S3 S4 S5 Test
Working standard (in ml) - 0.2 0.4 0.6 0.8 1.0 1.0
Concentration in std (in μg) - 400 800 1200 1600 2000 -
Water (in ml) 2 1.8 1.6 1.4 1.2 1.0 1.0
DNSA reagent (ml) 1 1 1 1 1 1 1
Boiling water bath At 90˚ C for 1.5 min.
Water (ml) 17 17 17 17 17 17 17
OD read at 540 nm

Dinitro salicylic acid (DNSA) method

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Dinitro salicylic acid (DNSA) method

1. Dinitrosalicylicacid(DNSA)method

2.

3.

4.

5. Particulars B S1 S2 S3 S4 S5 Test Working standard (in ml) - 0.2 0.4 0.6 0.8 1.0 1.0 Concentration in std (in μg) - 400 800 1200 1600 2000 - Water (in ml) 2 1.8 1.6 1.4 1.2 1.0 1.0 DNSA reagent (ml) 1 1 1 1 1 1 1 Boiling water bath At 90˚ C for 1.5 min. Water (ml) 17 17 17 17 17 17 17 OD read at 540 nm