2. Introduction
⢠Brucellosis is a worldwide zoonosis caused by the bacterial genus Brucella.
⢠These organisms localize in the reproductive organs of host animals, causing
abortions and sterility.
⢠They are shed in large numbers in the animal's urine, milk, and placental
fluid.
⢠Exposure to infected animals and animal products causes the disease in
humans.
3. Introduction
⢠Brucellosis was first diagnosed by Wright and Smith in 1897.
⢠Rose Bengal test detects antibodies.
⢠The synonyms of Brucellosis are:
⢠1.Undulant fever
⢠2.Malta fever.
⢠3.Gibraltar fever.
⢠4.Mediterranean fever
4. Rose Bengal Plate Test
⢠It is a rapid plate agglutination test by using a slide.
⢠Used in field for diagnosis of Human brucellosis and Bovine Brucellosis.
⢠It is actually a screening test and positive reaction samples should be
confirmed by STAT or CFT.
⢠Time taken for reaction is 2 minutes.
⢠Cheap and Faster.
⢠Synonyms: Rose Bengal Agglutinin Test, Rose Bengal Dye Test
7. Procedure
⢠A clean and sterilized slide is placed on a white background.
⢠Rose Bengal Dye stained Antigen vial should be gently swirled.
⢠With a micropipette two drops of antigen are taken on both ends of glass slide.
⢠An equal volume of test sera is added to the antigen.
⢠With a non-metallic stick, gently mix the antigen and sera.
⢠Rotate slide is slanted orbital motion.
⢠Rotate the slide with a mechanical rotator at 80-100 rpm for 2 minutes (if possible).
⢠Appearance of agglutinin clumps indicate positive reaction.
⢠Positive samples should be confirmed by STAT or CFT.
11. Result and Interpretation
⢠No agglutination= absence of specific antibodies
⢠Agglutination (even slight) = presence of specific antibodies
⢠If agglutination appear after 15 seconds = (1:640)
⢠If agglutination appear after 30 seconds = (1:320)
⢠If agglutination appear after 1 min. = (1:160)
⢠If agglutination appear after 1.30 min. = (1:80)