Production technique of true potato seed

ABHISHEK TIWARI
ABHISHEK TIWARIAgriculture Research um Chandra Shekhar Azad University of Agriculture & Technology Kanpur UP
COURSE SEMINAR On
Production technique of TRUE POTATO SEED
Presented by- Abhishek Pati Tiwari
(CA-10431/17)
Advisor- Dr. A. L. Jatav
Introduction
Potato (Solanum tuberosum L.) is major non-cereal
food crop, ranks 4th in area & production in the world
after wheat, maize & rice.
Originated in Andean region i.e. Southern Peru and
Bolivia where it is domesticated 7000-10000 year ago.
 Europeans introduced potato in India during late 16th
century or early 17th century
The Great Irish Famine, due to late blight in the 1845
which resulted in 1 million Irish people died and
another 1 million migrated.
TPS is practically pathogen free & quite easy for
transportation and storage.
Taxonomy & Morphology
 Family Solanaceae
 Important cultivated species
Solanum tuberosum ssp. tuberosum
Solanum tuberosum ssp. andigena
 2n= 48, tetraploid
 Cross pollinated
 About 60 cm high culm
 Green cherry fruits (300 seed in each)
 Potato stolons: lateral stems grow
horizontally
 Potato tubers: modified stem,
initiation of young tubers at the tip
of stolons at 35-40 DAP, process
called tuberization.
BOTANICAL DESCRIPTION
 It is vegetatively propagated by
means of tubers
 The leaves are alternate &
compound
 The root is adventitious,arising from
the base of a sprout
 The root growth is restricted upto
20-25 cm
 The flower of the potato are in
terminal cluster which contain five
stamens,two celled pistil,five sepals
&five petals
Area, Production & Productivity
 World
 Total Production = 368.1 m t
 China = 88.9 m t
 India = 48.53 m t
 Russia = 30.2 m t
 India
 Area = 2.13 m ha
 Production = 48.53 m t
 Productivity = 227.876 q/ha
 Utter Pradesh
 Area = 595200 ha
 Production = 14755000 t
 Productivity = 247.9 q/ha
1/3 of total produced by
China and India.
Major potato producing states:
U.P., W.B., Bihar, Punjab, Haryana
Source FAO 2016-17
www.indiastat.com
0
500000
1000000
1500000
2000000
2500000
3000000
3500000
4000000
4500000
5000000
2011
2012
2013
2014
2015
2016
Area & Production of potato in india
area (ha) prod.(mt)
Source- www.indiastat.com
34%
29%
13%
9%
7%
6%
2%
Some largest potato producing states
UP
WB
Bihar
Gujrat
MP
Punjab
Assam
Source- www.potatopro.com
SEED POTATO
 Potato tuber of definite size
are used for further
multiplication
 Should be free from all viral
diseases
 Should not have been grown
in areas where wart, cyst
nematode and other
quarantine diseases are
endemic
 Should be either free from
soil and tuber borne diseases
or carry them within
permissible limits
 Should be of proper
physiological age
Different Agronomic practices
involved in production of quality
seed1. Seed treatment
2. Seed rate (25-30)q/h
3. Seed size (4-6)cm or >40 g
4. Spacing (60x20)cm
5. Method of planting (3-4 cm depth)
6. Inter-culture (Earthing up etc.)
7. Irrigation
8. Fertilizer managemet (100-125N,80-100P,100-125K)
9. Weed management
10.Disease & pest management
11. Haulm cutting
Production technique of true potato seed
True Potato Seed Technology
What is TPS?
True Potato Seed (TPS) is
the actual botanical seed
produced by the sexual
reproduction, and is
formed inside the
fruits/berries, resembling
tomatoes. TPS is
occasionally formed after
the potato has finished
flowering.
True Potato seed is very effective & cheap source of
planting material. The shortage of good quality seed
tubers, high cost of seed, transportation problem,
virus infiltration in seed tubers are some of the
problems associated with the use of seed tubers as
planting material. The concept of raising potato crop
from true potato seeds (TPS) or botanical seeds was
conceived in India in the early fifties by Dr. S.
Ramanujan, the founder director of CPRI.
Paired-row concept for Cultivation
of True Potato Seed
 Paired-row concept for cultivation of True
Potato Seed (TPS) is the innovation in
which TPS are sown in narrow inter space
of 10 cm ×10 cm with 5 cm for plant to plant
distance. A total of 100 plants per
m2 were maintained in all cases.
 This innovation is less laborious than a
single row system, which need more time.
Very high level of seedling maintenance
is the major utility of this concept.
Selection of parents
Selection of parents is based on quantity of hybrid
TPS, which can be produced from per unit area from
heterosis. Usually, clones of S. tuberosum are used as
female and S. andigena as male that result in heterosis
with added advantage of high pollen load.
Planting of parents
 Cut pieces of tuber obtained from male and female
plants are planted in separate blocks with required
staggering period i.e. 7 days early planting of male
than the female to facilitate pollen availability.
 Recommended planting geometry for male is 60 x 20
cm an 50 x 15 cm for female
 The block with tuber of female parent is kept 1 m wide
with a walking space of 80 cm between blocks.
Light requirement
Potato plant require large photoperiodic condition for
flowering. In plains flowering can be achieved by
providing 5 hr of extra illumination during flowering
i.e. December-January from 4 to 9 pm with sodium
vapour lamp of 150 watt. One sodium bulb is sufficient
to cover an area of 100 sqm.
Pollination and fertilization
 To minimize duration of pollination, bud and flower
bunches are trimmed to remain 6 buds/branch.
 The mature male flower are collected in the evening
and stored over night at room tempreture for higher
recovery of pollens.
 In commercial seed production programme
pollination is done 2-3 times at interval of 8 hr without
emasculation to save the time.
 It allow 1-5% selfing.
Harvesting and processing
 Berries are picked after 45-50 day of pollination and
allow to ripen. A hand operated reverse screw juice
extractor is used to extract the seed.
 The seed and pulp is treated with 10% HCL with
continuous stirring for 20 minutes. The debris is
removed by nylon net of 3.13 mm mesh size.
 The seed is washed 3-4 times with water for complete
removal of HCL.
 Seeds is spread over a thin layer and dried under shade
for 72 hr and exposed to sunlight for 30 minutes so
that the moisture content up to 5-7% is maintained.
Packaging and storage
The seed is packed in double layred polythene bags
and stored at room temperature for short-term storage
and at 6-10 degree calcius for long term storage.
Seed sowing
 Freshly harvested TPS have dormancy of approximate
180 day therefore, storage for atleast a year is
recommended.
 Dormancy can be released by soaking the seed in 1500-
2000 ppm GA3 solution for 48 hr.
 The TPS are sown in nursery beds @ 2 g/sqm and
seedling are transplanted in field within 25 days.
 In heavy soil, TPS can be sown directly.
Production and utilization of TPS
Selection of parents (1st yrs)
Staggered planting of parental line
Flowering (40 DAP)
Pollination & fertilization
Initiation of berries (45-48 DAP)
Maturation of berries (75-80 DAP)
Harvesting of berries, seed extraction &
storage (80 DAP)
Planting of seed in nursery bed ( 2nd yrs)
Seedling tuber production (90 DAP)
Multiplication of seed
TPS Technology
TPS utilization technologies for
potato production
Using TPS, potato crop can be grown by two method
1- direct field sowing
2- Transplanting seedling to the field
3- Planting seedling tuber produced from TPS
Advantages of using True Potato
Seeds as planting material
100 grams is sufficient to cover one hectare instead
of planting 2.5-3.0 tons of Potato seed tuber.
 TPS has capability of giving more production.
 No cold storage facility is required for storing TPS
 Comparatively more resistance to pests and diseases.
 Net profit is more as cost of cultivation is less and also
as per hectare production is much more.
 The seed tubers being utilized could be otherwise
used for consumption.
Some varieties of true potatoseeds:-
Some of the important true potatoes seeds
(TPS) varieties are-
HPS 1/13,HPS II/63, TPS C-3, 92-PT-27 etc
General Seed Certification
Standards for Seed Potato
I. Classification :
1. Hill Seed (HS): 2500 m ASL2. Plains seed (PS): 2500m BSL
II. Land Requirements : not infested with wart , cyst forming
nematodes, brown rot within the previous 3 years or common
scab.
III. Field Inspection: Minimum 4 inspections :
First inspection:
In the hills- 45 DAP
In Plains- 35 DAP
Second inspection: 60-70 DAP
Third inspection: Immediately after haulms cutting/destruction
Fourth inspection: 10 days after haulms cutting/destruction
IV. Isolation: 5 m for FS and CS
V. Isolation for TPS : 200m
Production technique of true potato seed
Potato varieties developed during last 5 years (8)
to NorthAdapted
Indian plains Yield
potential of 40 t/ha
Resistance to early
and late blight, early
bulker, suitable for
early planting.
Adapted to UP
and adjoining
areas Yield
potential of 40
t/ha Resistance
to late blight,
early bulker.
Kufri Garima
Adapted to Indo-
gangetic plains and
plateau region
Yield potential of35
t/ha
Field resistant tolate
blight
Kufri Gaurav
Adapted to North
Indian plains Yield
potential of 40 t/ha
Perform well under
sub-optimal doses
of fertilizers )
Kufri SadabaharKufri Khyati TABLEVARIETIES (4)
Kufri Frysona
Adapted to North
Indian plains
Yield potential of40
t/ha
Resistance to late
blight and wart,
suitable formaking
French fries
Adapted to Hills
(Processing variety)
Yield potential of 35
t/ha Resistance to late
blight and wart
Kufri Himsona
Potato varieties developed during last 5 years…contd.
PROCESSING VARIETIES (2)
Kufri Girdhari
Adapted to Indian
Hills Yield potential of
35 t/ha Extreme
resistance to late
blight (Joseph et al.,
2010)
Kufri Neelima
Adapted to
Southern Hills
Yield potential of
30 t/ha Resistance
to early blight, late
blight and cyst
nematode (Annual
Report 2009-10,
pp.107)
DISEASE RESISTANTVARIETIES (2)
Potato Research in India
 Central Potato Research Institute,
Shimla (CPRI) established in 1949
 It has 7 research centres
• CPRIC, Modipuram, Meerut (UP)
• CPRS, Jalandhar (Punjab)
• CPRS, Gwalior (MP)
• CPRS, Patna (Bihar)
• CPRS, Kufri-Fagu, Shimla (HP)
• CPRS, Udagamandalam (Tamilnadu)
•CPRS, Shillong (Meghlaya)
 All India Coordinated Reseaech Project
Potato (AICRP),1970
 has headquarter at CPRI, Shimla has a
nationwide network of 25 centers (7 CPRI
based centers, 17 SAU based centers and 1
Voluntary center)
Production technique of true potato seed
Scope of this technique
In india seed replacement rate of potato crop is ~13%.
So there is a need to increase SR but by potato tuber it
can not be achieve because of their transportation
cost, less availability, requirement of care during
storage etc. For increase of SRR there is a lot of scope
for TPS because it required in less amount, easy to
transport and store.
Conclusion
`It may be concluded that this technique is used for
disease and virus free seed production. The viral
infiltration in the seed tuber is also less. The cost of
tuber treating chemicals is also reduced and requires
very little space for storage. This technique can be used
to checked the diseases to new areas.
Production technique of true potato seed
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Production technique of true potato seed

  • 1. COURSE SEMINAR On Production technique of TRUE POTATO SEED Presented by- Abhishek Pati Tiwari (CA-10431/17) Advisor- Dr. A. L. Jatav
  • 2. Introduction Potato (Solanum tuberosum L.) is major non-cereal food crop, ranks 4th in area & production in the world after wheat, maize & rice. Originated in Andean region i.e. Southern Peru and Bolivia where it is domesticated 7000-10000 year ago.  Europeans introduced potato in India during late 16th century or early 17th century The Great Irish Famine, due to late blight in the 1845 which resulted in 1 million Irish people died and another 1 million migrated. TPS is practically pathogen free & quite easy for transportation and storage.
  • 3. Taxonomy & Morphology  Family Solanaceae  Important cultivated species Solanum tuberosum ssp. tuberosum Solanum tuberosum ssp. andigena  2n= 48, tetraploid  Cross pollinated  About 60 cm high culm  Green cherry fruits (300 seed in each)  Potato stolons: lateral stems grow horizontally  Potato tubers: modified stem, initiation of young tubers at the tip of stolons at 35-40 DAP, process called tuberization.
  • 4. BOTANICAL DESCRIPTION  It is vegetatively propagated by means of tubers  The leaves are alternate & compound  The root is adventitious,arising from the base of a sprout  The root growth is restricted upto 20-25 cm  The flower of the potato are in terminal cluster which contain five stamens,two celled pistil,five sepals &five petals
  • 5. Area, Production & Productivity  World  Total Production = 368.1 m t  China = 88.9 m t  India = 48.53 m t  Russia = 30.2 m t  India  Area = 2.13 m ha  Production = 48.53 m t  Productivity = 227.876 q/ha  Utter Pradesh  Area = 595200 ha  Production = 14755000 t  Productivity = 247.9 q/ha 1/3 of total produced by China and India. Major potato producing states: U.P., W.B., Bihar, Punjab, Haryana Source FAO 2016-17 www.indiastat.com
  • 7. 34% 29% 13% 9% 7% 6% 2% Some largest potato producing states UP WB Bihar Gujrat MP Punjab Assam Source- www.potatopro.com
  • 8. SEED POTATO  Potato tuber of definite size are used for further multiplication  Should be free from all viral diseases  Should not have been grown in areas where wart, cyst nematode and other quarantine diseases are endemic  Should be either free from soil and tuber borne diseases or carry them within permissible limits  Should be of proper physiological age
  • 9. Different Agronomic practices involved in production of quality seed1. Seed treatment 2. Seed rate (25-30)q/h 3. Seed size (4-6)cm or >40 g 4. Spacing (60x20)cm 5. Method of planting (3-4 cm depth) 6. Inter-culture (Earthing up etc.) 7. Irrigation 8. Fertilizer managemet (100-125N,80-100P,100-125K) 9. Weed management 10.Disease & pest management 11. Haulm cutting
  • 11. True Potato Seed Technology What is TPS? True Potato Seed (TPS) is the actual botanical seed produced by the sexual reproduction, and is formed inside the fruits/berries, resembling tomatoes. TPS is occasionally formed after the potato has finished flowering.
  • 12. True Potato seed is very effective & cheap source of planting material. The shortage of good quality seed tubers, high cost of seed, transportation problem, virus infiltration in seed tubers are some of the problems associated with the use of seed tubers as planting material. The concept of raising potato crop from true potato seeds (TPS) or botanical seeds was conceived in India in the early fifties by Dr. S. Ramanujan, the founder director of CPRI.
  • 13. Paired-row concept for Cultivation of True Potato Seed  Paired-row concept for cultivation of True Potato Seed (TPS) is the innovation in which TPS are sown in narrow inter space of 10 cm ×10 cm with 5 cm for plant to plant distance. A total of 100 plants per m2 were maintained in all cases.  This innovation is less laborious than a single row system, which need more time. Very high level of seedling maintenance is the major utility of this concept.
  • 14. Selection of parents Selection of parents is based on quantity of hybrid TPS, which can be produced from per unit area from heterosis. Usually, clones of S. tuberosum are used as female and S. andigena as male that result in heterosis with added advantage of high pollen load.
  • 15. Planting of parents  Cut pieces of tuber obtained from male and female plants are planted in separate blocks with required staggering period i.e. 7 days early planting of male than the female to facilitate pollen availability.  Recommended planting geometry for male is 60 x 20 cm an 50 x 15 cm for female  The block with tuber of female parent is kept 1 m wide with a walking space of 80 cm between blocks.
  • 16. Light requirement Potato plant require large photoperiodic condition for flowering. In plains flowering can be achieved by providing 5 hr of extra illumination during flowering i.e. December-January from 4 to 9 pm with sodium vapour lamp of 150 watt. One sodium bulb is sufficient to cover an area of 100 sqm.
  • 17. Pollination and fertilization  To minimize duration of pollination, bud and flower bunches are trimmed to remain 6 buds/branch.  The mature male flower are collected in the evening and stored over night at room tempreture for higher recovery of pollens.  In commercial seed production programme pollination is done 2-3 times at interval of 8 hr without emasculation to save the time.  It allow 1-5% selfing.
  • 18. Harvesting and processing  Berries are picked after 45-50 day of pollination and allow to ripen. A hand operated reverse screw juice extractor is used to extract the seed.  The seed and pulp is treated with 10% HCL with continuous stirring for 20 minutes. The debris is removed by nylon net of 3.13 mm mesh size.  The seed is washed 3-4 times with water for complete removal of HCL.  Seeds is spread over a thin layer and dried under shade for 72 hr and exposed to sunlight for 30 minutes so that the moisture content up to 5-7% is maintained.
  • 19. Packaging and storage The seed is packed in double layred polythene bags and stored at room temperature for short-term storage and at 6-10 degree calcius for long term storage.
  • 20. Seed sowing  Freshly harvested TPS have dormancy of approximate 180 day therefore, storage for atleast a year is recommended.  Dormancy can be released by soaking the seed in 1500- 2000 ppm GA3 solution for 48 hr.  The TPS are sown in nursery beds @ 2 g/sqm and seedling are transplanted in field within 25 days.  In heavy soil, TPS can be sown directly.
  • 21. Production and utilization of TPS Selection of parents (1st yrs) Staggered planting of parental line Flowering (40 DAP) Pollination & fertilization Initiation of berries (45-48 DAP)
  • 22. Maturation of berries (75-80 DAP) Harvesting of berries, seed extraction & storage (80 DAP) Planting of seed in nursery bed ( 2nd yrs) Seedling tuber production (90 DAP) Multiplication of seed
  • 24. TPS utilization technologies for potato production Using TPS, potato crop can be grown by two method 1- direct field sowing 2- Transplanting seedling to the field 3- Planting seedling tuber produced from TPS
  • 25. Advantages of using True Potato Seeds as planting material 100 grams is sufficient to cover one hectare instead of planting 2.5-3.0 tons of Potato seed tuber.  TPS has capability of giving more production.  No cold storage facility is required for storing TPS  Comparatively more resistance to pests and diseases.  Net profit is more as cost of cultivation is less and also as per hectare production is much more.  The seed tubers being utilized could be otherwise used for consumption.
  • 26. Some varieties of true potatoseeds:- Some of the important true potatoes seeds (TPS) varieties are- HPS 1/13,HPS II/63, TPS C-3, 92-PT-27 etc
  • 27. General Seed Certification Standards for Seed Potato I. Classification : 1. Hill Seed (HS): 2500 m ASL2. Plains seed (PS): 2500m BSL II. Land Requirements : not infested with wart , cyst forming nematodes, brown rot within the previous 3 years or common scab. III. Field Inspection: Minimum 4 inspections : First inspection: In the hills- 45 DAP In Plains- 35 DAP Second inspection: 60-70 DAP Third inspection: Immediately after haulms cutting/destruction Fourth inspection: 10 days after haulms cutting/destruction IV. Isolation: 5 m for FS and CS V. Isolation for TPS : 200m
  • 29. Potato varieties developed during last 5 years (8) to NorthAdapted Indian plains Yield potential of 40 t/ha Resistance to early and late blight, early bulker, suitable for early planting. Adapted to UP and adjoining areas Yield potential of 40 t/ha Resistance to late blight, early bulker. Kufri Garima Adapted to Indo- gangetic plains and plateau region Yield potential of35 t/ha Field resistant tolate blight Kufri Gaurav Adapted to North Indian plains Yield potential of 40 t/ha Perform well under sub-optimal doses of fertilizers ) Kufri SadabaharKufri Khyati TABLEVARIETIES (4)
  • 30. Kufri Frysona Adapted to North Indian plains Yield potential of40 t/ha Resistance to late blight and wart, suitable formaking French fries Adapted to Hills (Processing variety) Yield potential of 35 t/ha Resistance to late blight and wart Kufri Himsona Potato varieties developed during last 5 years…contd. PROCESSING VARIETIES (2) Kufri Girdhari Adapted to Indian Hills Yield potential of 35 t/ha Extreme resistance to late blight (Joseph et al., 2010) Kufri Neelima Adapted to Southern Hills Yield potential of 30 t/ha Resistance to early blight, late blight and cyst nematode (Annual Report 2009-10, pp.107) DISEASE RESISTANTVARIETIES (2)
  • 31. Potato Research in India  Central Potato Research Institute, Shimla (CPRI) established in 1949  It has 7 research centres • CPRIC, Modipuram, Meerut (UP) • CPRS, Jalandhar (Punjab) • CPRS, Gwalior (MP) • CPRS, Patna (Bihar) • CPRS, Kufri-Fagu, Shimla (HP) • CPRS, Udagamandalam (Tamilnadu) •CPRS, Shillong (Meghlaya)  All India Coordinated Reseaech Project Potato (AICRP),1970  has headquarter at CPRI, Shimla has a nationwide network of 25 centers (7 CPRI based centers, 17 SAU based centers and 1 Voluntary center)
  • 33. Scope of this technique In india seed replacement rate of potato crop is ~13%. So there is a need to increase SR but by potato tuber it can not be achieve because of their transportation cost, less availability, requirement of care during storage etc. For increase of SRR there is a lot of scope for TPS because it required in less amount, easy to transport and store.
  • 34. Conclusion `It may be concluded that this technique is used for disease and virus free seed production. The viral infiltration in the seed tuber is also less. The cost of tuber treating chemicals is also reduced and requires very little space for storage. This technique can be used to checked the diseases to new areas.