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Asexual Propagation
Asexual Propagation 
– controlled perpetuation of plants. 
Its objective is to achieve an 
increase in number to preserve the 
essential characteristic of a plant.
Reasons/Importance 
–Production of true to type plant (genetically 
identical) 
–Propagation of plants that does not 
generally produce seeds (breadfruit) 
–Propagation of plants that produce seed but 
are difficult to germinate (banana, abaca) 
–Shorter maturity period of plants/early 
bearing 
–Production of uniform asexual rootstock
Characteristics of a good rootstock 
• good root system 
• resistant to soil-borne-disease 
• resistant to drought
Methods of asexual propagation 
• Cutting – propagation technique where in a vegetable 
parts is detached/removed from the mother plant and 
is subjected to a condition favorable for the 
regeneration of roots and production of shoots. 
• Air layering/marcotting – adventitious roots are 
caused to form on stem while still attached to the 
mother plant. 
• Garfting – art of connecting or joining two plant parts 
and allowing it to unite and grow as one plant. 
• Budding – a form of grafting where in the scion is 
limited only to a single bud.
Air Layering 
• Principle of air layering/marcotting 
Restricting the flow or interruption of the 
downward movement of organic materials 
(CHO, growth regulators and other growth 
factors) synthesized from the leaves and 
growing shoot tips, accumulating those 
materials above the wound thereby 
stimulating rooting.
Advantage of Marcotting over Cutting 
• Stress avoidance – treated branch is still 
supplied with water and nutrients by the 
mother plant. 
• Disadvantages: 
– Requires more skill 
– Needs more time 
– More complicated 
– Requires other materials
Factors affecting rooting of layered 
plants 
• skill of the propagator – branch selection 
• removal of the cambium layer (composed of actively 
dividing tissues) which when not completely remove 
will regenerate to phloem vessels or re-growth of the 
bark. 
• condition of the mother plant (vegetative stage) 
• rooting medium – characteristics of a good rooting 
medium: a) good aeration, b) good moisture supply, c) 
able to supply nutrients 
• wrapping materials – to prevent moisture loss, must 
be transparent, stable and strong 
• treatment – growth regulators e.g. ANAA, IBA, IAA
Plant cell characteristics that make 
propagation by cutting possible 
Totipotency – a plant cell characteristic by which 
a single living vegetative cell contains all the 
genetic information needed to regenerate an 
entire plant. 
Dedifferentiation – a plant cell characteristics by 
which a mature or differentiated cell could 
return back to meristematic condition.
Materials Needed 
• rooting medium – create an environment similar 
to the soil (dark environment, moisture, nutrient, 
aeration). 
• cellophane – any transparent material for the 
ease in checking emerging shoots 
• a good and healthy mother plant 
• tying material 
• sharp knife or cutter 
• root inducer (for difficult to root and expensive 
planting material).

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Air layering - asexual propagation

  • 2. Asexual Propagation – controlled perpetuation of plants. Its objective is to achieve an increase in number to preserve the essential characteristic of a plant.
  • 3. Reasons/Importance –Production of true to type plant (genetically identical) –Propagation of plants that does not generally produce seeds (breadfruit) –Propagation of plants that produce seed but are difficult to germinate (banana, abaca) –Shorter maturity period of plants/early bearing –Production of uniform asexual rootstock
  • 4. Characteristics of a good rootstock • good root system • resistant to soil-borne-disease • resistant to drought
  • 5. Methods of asexual propagation • Cutting – propagation technique where in a vegetable parts is detached/removed from the mother plant and is subjected to a condition favorable for the regeneration of roots and production of shoots. • Air layering/marcotting – adventitious roots are caused to form on stem while still attached to the mother plant. • Garfting – art of connecting or joining two plant parts and allowing it to unite and grow as one plant. • Budding – a form of grafting where in the scion is limited only to a single bud.
  • 6. Air Layering • Principle of air layering/marcotting Restricting the flow or interruption of the downward movement of organic materials (CHO, growth regulators and other growth factors) synthesized from the leaves and growing shoot tips, accumulating those materials above the wound thereby stimulating rooting.
  • 7. Advantage of Marcotting over Cutting • Stress avoidance – treated branch is still supplied with water and nutrients by the mother plant. • Disadvantages: – Requires more skill – Needs more time – More complicated – Requires other materials
  • 8. Factors affecting rooting of layered plants • skill of the propagator – branch selection • removal of the cambium layer (composed of actively dividing tissues) which when not completely remove will regenerate to phloem vessels or re-growth of the bark. • condition of the mother plant (vegetative stage) • rooting medium – characteristics of a good rooting medium: a) good aeration, b) good moisture supply, c) able to supply nutrients • wrapping materials – to prevent moisture loss, must be transparent, stable and strong • treatment – growth regulators e.g. ANAA, IBA, IAA
  • 9. Plant cell characteristics that make propagation by cutting possible Totipotency – a plant cell characteristic by which a single living vegetative cell contains all the genetic information needed to regenerate an entire plant. Dedifferentiation – a plant cell characteristics by which a mature or differentiated cell could return back to meristematic condition.
  • 10. Materials Needed • rooting medium – create an environment similar to the soil (dark environment, moisture, nutrient, aeration). • cellophane – any transparent material for the ease in checking emerging shoots • a good and healthy mother plant • tying material • sharp knife or cutter • root inducer (for difficult to root and expensive planting material).