Biotech 2011-08-recombinant-dna

Cloning Overview ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Libraries ,[object Object],[object Object],[object Object],[object Object]

Three general types of libraries ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Genomic Library ,[object Object],[object Object],[object Object],[object Object],[object Object]

cDNA Libraries ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Expression libraries ,[object Object],[object Object],[object Object],[object Object],[object Object]

Why clone? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Cloning reagents ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

DNA ligase ,[object Object],[object Object],[object Object],[object Object],[object Object]

Reverse transcriptase ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Vectors ,[object Object],[object Object],[object Object],[object Object],[object Object]

PBR322 ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

The host ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Colony screens ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

What to do with the DNA? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Examples of medical relevance ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Cloning insulin ,[object Object],[object Object],[object Object],[object Object]

cDNA clones were necessary ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Identifying the insulin cDNA clone ,[object Object],[object Object],[object Object],[object Object]

Expressing the clone ,[object Object],[object Object],[object Object],[object Object]

Problems with expressing eukaryotic genes in a bacterium ,[object Object],[object Object],[object Object]

Another approach ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Reconstitution of insulin from individual chains ,[object Object],[object Object],[object Object],[object Object]

Present day ,[object Object],[object Object],[object Object],[object Object]

Gene therapy for IDDM type I? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Beta cell regulation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Other human diseases potentially amenable to gene therapy ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Biotech 2011-08-recombinant-dna

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Biotech 2011-08-recombinant-dna