Importance of plantibodies

1. Welcome

2. Plantibodies
Presented by:-
KAMBLE A.P.
7th sem
(BED11ABT15B)

3. What are plantibodies ??
 A plantibody is a Antibody
produce by genetically modified
plant.
 Antibody are part of Animal
immune system, and produce in
plant by transforming them with
Antibody genes from Animals.

4. Cont….
 This was first done in 1989,
with a mouse antibody made
by Tobacco plant.
 Although plant do not
naturally make antibody,
Plantibody have been shown
to function in the same way
as normal antibodies.

5. Antibody factories
 Plant are being used as antibody factories,
using their endomembrane and secretory
system to produce large amount of clinically
visible proteins.
 Antibodies can be expressed in plant as either
full –length molecules or as smaller fragment.

7. Methods for plantibody production
 Using transformation
and translent
expression to introduce
new genes into a host
cell.
 Targetting to the
APOPLASM by the
tagging with a small
peptide sequence.

8. Cont….
 Single molecule added to target the
protein to Endoplasmic Reticulum
(E.R); to ensure correct folding of the
protein.
 Higher protein level obtained in the
E.R and the apoplasm as compared to
the crystol.

9. Purification of plantibodies
 Purification can be purified
cheaply in large quantity
 Transgenic seeds assure
excellent storage properties and
due to limited range of
endogenous proteins in seed,
separation of plantibodies is less
complicated
 There is no risk of srading animal
disease to human as the
antibody produced by plants.

10. Technique for purification of
plantibodies
 Filtration
 Immunofluoresence
 Chromatography
 Difiltration
 Polymer fusion.

11. Evaluation technique for the
Plantibodies:-
 RIA (Radioimmunoassay)
 ELISA (Enzyme linked
immuno sorbant assay)
 Immunofluorence
southern blot analysis.

12. Production cost of Plantibodies

13. Advantage:-
 Large-scale production of plantibodies for
immunotherapy
 Benificial to human, plants as well as
economy.
 No risk of transmitting diseases to human
 Eliminate the toxic compound;

14. Application:-
 Antibodies generated by plant are
cheaper, easier to manage, and safer
to use than those obtained from
animals.
 Treatment of illness such as immune
disorder, cancer and inflammatory
disease.
 Plantibodies are close to passing
clinical trail.

15. Tobacco-derived ‘’PLANTIBODIES’’ enter the fight
against Ebola.