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Proteins 
 are compounds of carbon, hydrogen, oxygen, 
nitrogen and sometimes sulphur, phosphorus 
 amino acids: 
– unit of proteins 
– about 20 different types 
– essential and non-essential types
An amino acid molecules 
H2N 
amino group 
COOH 
carboxyl group 
R 
C 
H
The condensation and hydrolysis of a 
polypeptide 
1 4 
condensation hydrolysis 
1 2 3 4 5
Condensation of two amino acid to form a 
dipeptide 
R1 
H2N C 
H 
C 
O 
OH H 
N 
H 
H 
C 
R2 
COOH 
R1 
H2N C 
H 
O 
C---N COOH 
H 
H 
C 
R2 
H2O
Functions of proteins 
 components of cell membranes and cytoplasm 
 for growth and repair of body cells 
 for making enzymes, hormones, antibodies and 
haemoglobin 
 provide energy during starvation 
 excess protein is under deamination in the liver 
 deficiency disease: Kwashiorkor
Test for proteins / Biuret test 
egg white solution 
1 cm3 NaOH solution 
Put CuSO2 solution drop by 
drop, and shake the mixture 
after addition of each drop 
Positive result: purple colour

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P 10 proteins

  • 1. Proteins  are compounds of carbon, hydrogen, oxygen, nitrogen and sometimes sulphur, phosphorus  amino acids: – unit of proteins – about 20 different types – essential and non-essential types
  • 2. An amino acid molecules H2N amino group COOH carboxyl group R C H
  • 3. The condensation and hydrolysis of a polypeptide 1 4 condensation hydrolysis 1 2 3 4 5
  • 4. Condensation of two amino acid to form a dipeptide R1 H2N C H C O OH H N H H C R2 COOH R1 H2N C H O C---N COOH H H C R2 H2O
  • 5. Functions of proteins  components of cell membranes and cytoplasm  for growth and repair of body cells  for making enzymes, hormones, antibodies and haemoglobin  provide energy during starvation  excess protein is under deamination in the liver  deficiency disease: Kwashiorkor
  • 6. Test for proteins / Biuret test egg white solution 1 cm3 NaOH solution Put CuSO2 solution drop by drop, and shake the mixture after addition of each drop Positive result: purple colour