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GES 102 Lab #5 Spring 2015 20 GES 102 - PHOTOSYNTHESIS LAB This week you will investigate the effect of light intensity on the rate of photosynthesis in spinach leaves. Fresh spinach bought in the store is green, and the leaves still have the ability to do photosynthesis. Photosynthesis produces carbohydrates and oxygen and we will use the rate of oxygen production as an indicator of how quickly the spinach leaves actually do photosynthesis. You will use a hole punch to make a number of small disks of spinach. These disks float when they contain oxygen from photosynthesis. By applying vacuum to the small spinach disks, the oxygen is forced out, and they sink. When you then place the disks in a transparent cup with water, and expose them to light, they will produce oxygen and eventually float. The time it takes the small disks to float is a measure of photosynthesis, and you will test the effect of different light intensities on the floatation time. Background: In your first lab this semester you investigated how the UV portion of sunlight was able to cause harm to living cells, which is not so surprising since this shorter wavelength carry enough energy to damage the cell’s DNA. In this week’s lab we will instead use the portion of radiation from the sun that we call daylight (380-780 nm), plenty of which makes it down to the earth’s surface. Our eyes are sensitive to this portion of the spectrum, and it’s also the portion plants use for photosynthesis. The process of photosynthesis can be simplified as: CO2 + H2O sugar + O2. It is really divided up into two sets of reactions, the Light Reaction and the Calvin Cycle. In the Light Reaction the plant cells use chlorophyll to capture some of the energy in sunlight. When sunlight hits a chlorophyll molecule, electrons in the chlorophyll GES 102 Lab #5 Spring 2015 21 molecule are excited, they ‘spin’ faster because of the absorbed energy. These excited electrons are passed on down the line and chlorophyll will take a continuous supply of new electrons from H2O which is broken down, producing O2. The energy in the excited electrons is eventually used to produce two high energy molecules, called ATP and NADPH. In the next step, the Calvin Cycle, the energy contained in ATP and NADPH is used to help take CO2 (carbon dioxide) from the air and manufacture sugar. Spinach plants normally get their CO2 from the air, through small openings on the underside of their leaves, called stomata. In our experiment some sodium bicarbonate (NaHCO3) will be added to the water, and provide the carbon needed for the small spinach disks to do photosynthesis. The O2 produced will be trapped inside the spinach disks and increase their buoyancy until they float. Alongside photosynthesis, the plants also do cellular respiration. They certainly are autotrophs and can produce their own “food”, the sugar. ...
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GES 102 Lab #5 Spring 2015 20 GES 102 - PHOTOSYNTHESIS LAB This week you will investigate the effect of light intensity on the rate of photosynthesis in spinach leaves. Fresh spinach bought in the store is green, and the leaves still have the ability to do photosynthesis. Photosynthesis produces carbohydrates and oxygen and we will use the rate of oxygen production as an indicator of how quickly the spinach leaves actually do photosynthesis. You will use a hole punch to make a number of small disks of spinach. These disks float when they contain oxygen from photosynthesis. By applying vacuum to the small spinach disks, the oxygen is forced out, and they sink. When you then place the disks in a transparent cup with water, and expose them to light, they will produce oxygen and eventually float. The time it takes the small disks to float is a measure of photosynthesis, and you will test the effect of different light intensities on the floatation time. Background: In your first lab this semester you investigated how the UV portion of sunlight was able to cause harm to living cells, which is not so surprising since this shorter wavelength carry enough energy to damage the cell’s DNA. In this week’s lab we will instead use the portion of radiation from the sun that we call daylight (380-780 nm), plenty of which makes it down to the earth’s surface. Our eyes are sensitive to this portion of the spectrum, and it’s also the portion plants use for photosynthesis. The process of photosynthesis can be simplified as: CO2 + H2O sugar + O2. It is really divided up into two sets of reactions, the Light Reaction and the Calvin Cycle. In the Light Reaction the plant cells use chlorophyll to capture some of the energy in sunlight. When sunlight hits a chlorophyll molecule, electrons in the chlorophyll GES 102 Lab #5 Spring 2015 21 molecule are excited, they ‘spin’ faster because of the absorbed energy. These excited electrons are passed on down the line and chlorophyll will take a continuous supply of new electrons from H2O which is broken down, producing O2. The energy in the excited electrons is eventually used to produce two high energy molecules, called ATP and NADPH. In the next step, the Calvin Cycle, the energy contained in ATP and NADPH is used to help take CO2 (carbon dioxide) from the air and manufacture sugar. Spinach plants normally get their CO2 from the air, through small openings on the underside of their leaves, called stomata. In our experiment some sodium bicarbonate (NaHCO3) will be added to the water, and provide the carbon needed for the small spinach disks to do photosynthesis. The O2 produced will be trapped inside the spinach disks and increase their buoyancy until they float. Alongside photosynthesis, the plants also do cellular respiration. They certainly are autotrophs and can produce their own “food”, the sugar. ...
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Harrisburg weather watch2
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Harrisburg Weather Watch
Real Life Weather Phenomena In the classroom
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goes with the rainmaker station.
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(Precipitation) Earth’s Water
Cycle
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